Publication Date:
2021-05-19
Description:
In order to investigate the effect of ethanol extracts of garlic (Allium sativum) and Zataria multiflora on
Aeromonas hydrophila bacteria Persian sturgeon (Acipenser persicus)took the kids to the isolation and
identification of bacteria , the minimal inhibitory concentration (MIC) and minimal bactericidal concentration
(MBC) of bacteria Aeromonas hydrophila by garlic (Allium sativum) and Zataria multiflora hydroalchoholic
extracts to determine the lethal concentrations of hydroalchoholic extracts of garlic (Allium sativum) and Zataria
multiflora hydroalchoholic extracts on Persian sturgeon (Acipenser persicus)children , and also to evaluate the
efficacy and determine the effective doses of the bacterium Aeromonas hydrophila extracts of in vitro and in
vivo were measured.Due to the absence of the Aeromonas hydrophila identification by molecular country in the
study of bacteria isolated from sturgeon disease is suspected after detection by screening DNA extraction and
molecular By toward action and results by NSBI Aeromonas hydrophila bacteria and Authentication Code NSBI
was recorded in Gen Bank JX987090 . Based on studies done in vitro (in vitro) in this study, the minimum
inhibitory concentrations (MIC) Aeromonas hydrophila bacteria by extracts of garlic and thyme and arrange 1
mg/ml, 0.25mg/ml and the minimum bactericidal concentration (MBC) of bacteria Aeromonas hydrophila by the
extracts, respectively , and 2mg/ml, 0.5mg/ml.
Study on lethal concentration (LC50) of Zataria multiflora hydroalchoholic extract on fingerlings of Persian
sturgeon showed that during 96h and 1h, the LC50 was 766.65 and 9933.44 mg/L, respectively. Also, LC50 of
garlic extract during 96h and 1h was 1279.97 and 12624.08 mg/L, respectively. Investigation on white blood
cells (WBC) showed significant difference in lymphocyte and neutrophil numbers in different treatments
(P〈0.05). But, there was no significant difference in monocyte and eosinophil numbers in different treatments
(P〉0.05).
In this study, concentrations ranging from 400 to 1,000 mg/ml of hydroalchoholic extracts of Zataria multfor
treating Persian sturgeon (Acipenser persicus) infected with the Aeromonas hydrophila by intraperitoneal
injection were determined. Based on the results of the concentration of the extract to 800 mg/ml during shower
hour was determined . Assay to determine the concentration of garlic extract on the bacteria in the Persian
sturgeon (Acipenser persicus)concentration range of 600 to 1200 mg/ml determine the effective concentration of
extract equivalent to 1,000 mg/ml during shower hour was calculated.
Study on the pictures taken out from sections of gill, liver and kidny of Persian sturgeon fingerlings (Acipenser
persicus) showed that in different doses of garlic (Allium sativum) and Zataria multiflora hydroalchoholic
extracts the teretment grups were examined and some microscopic damages observed. They are hyperemia,
adhesion in the gill filaments, cell necrosis, melanin pigments in gill primary filaments, cloudy swelling of
hepatocytes, liver necrosis, hyperemia and increase in melanin pigments and melano macrophage centers in
liver, glomerular changes such as congestion and blocked the dilation of Bowman's space , bleeding, cell
necrosis , cloudy swelling of the in kidny.
Description:
Iranian Fisheries Science Research Institute
Description:
Published
Keywords:
Allium sativum
;
Zataria multiflora
;
Aeromonas hydrophila
;
Acipenser persicus
;
Aeromonas hydrophila
;
Kidny
;
Fish
;
Garlic
;
Fingerling
Repository Name:
AquaDocs
Type:
Report
,
Refereed
Format:
82pp.
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