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  • Species  (4)
  • Accreditation  (2)
  • 1
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    Unknown
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Publication Date: 2021-05-19
    Description: Chromosome number and karyotype of four important Barbus species of Iran were analysed. Thirty individual of B.grypus, 30 from B.sharpeyi, 28 of B.esocinus and 44 of B.pectoralis weret ested. To provide the chromosomal spreads, the kidney and gill arches of fish were sampled according to Bloom & Kligerman (1977). Barbus sharpeyi diploid model of chromosomes was 2n=98 containing 3 pairs of metacentric, 23 pairs of submetacentric,2 pairs of subtelocentric and 21 pairs of acro & telo- centric. Barbus grypus diploid model of chromosomes was also 2n=98. It contains 1 pair of metacentric, 22 pairs of submetacentric, 1 pair of subtelocentric and 25 pairs of acro & telocentric chromosomes. Barbus esocinus diploid model of chromosomes was 2n=98. It contains 7 pair of metacentric, 13 pairs of submetacentric, and 29 pairs of subtelo,acro & telocentric chromosomes. Barbus pectoralis diploid model of chromosomes was 2n=98. It contains 6 pair of metacentric, 16 pairs of submetacentric, and 27 pairs of subtelo,acro & telocentric chromosomes.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Cytogenetic ; Species ; Gene ; Barbus ; B.grypus ; B.sharpeyi ; B.esocinus ; B.pectoralis ; Barbus pectoralis
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 61pp.
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  • 2
    Publication Date: 2021-05-19
    Description: In order to primery research on population genetic and determination of probale polymorphisme in Giant Freshwater prawn of IRAN samples of muscle texture of this prawn in order to determination of the whole protein separated and transfered to biotechnology laboratory of khuzestan research center(2002-2003) this laboratory by the method of electrophoresis samples(Laemmli -SDS/Polyacrylamide Gel Electrophoresis) went under analysis stage.It is necessery to said that in these expreiments utilized from acrylamid gel by 12% and of the average samples were 10% of population of each center . As electrophoresis comparison shows population this prawn in IRAN is uniform and needs to imposing a similar genetic management through the country. Acording to calculated covariance table and also path way method of determining inbreeding coefficient(88.8%), inbreeding level (in the 10th generation) in which is as strange reason in alleviation of efficiency of this prawn in IRAN and danger sign for this society of prawns and with do attention reproduction of 10 generation research center in iran and adaptation with climatic and ecological circumstances in the country, should said Iranian Giant Freshwater Prawn.We have to call this society as Giant Freshwater prawn and by imposing exhabitate genetic reactional measures at is possible after for controlled generation and evaluation of results and also by calculation and controlling amount of inbreeding in the level of 3% discover the most hybrid of this naturalized species with foreign improved species and by culturing relationship of superior generation prevent from causing inbreeding problems during next year.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Macrobrachium rosenbergii ; Breeding ; Species ; Freshwater ; Prawn ; Giant Freshwater prawn ; Inbreeding
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 35pp.
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  • 3
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    Unknown
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25779 | 18721 | 2018-10-13 08:34:12 | 25779 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Genetic studies and gene banks preparation can identify guidelines for improving diversity and population structure and estimation, poaching and also the amount of cross breeding and provide genetic classification. In this study, sampling was performed from the important studied species habitats areas for the species such as: Dasyatis bennetti، Netuma thalassina, Netuma bilineata Carcharhinus leucas، Choerodon robustus، Pseudorhombus pentophthalmus ،Pseudorhombus arsius Thunnus tonggol ،Euthynnus affinis and Trichiurus lepturus. Total DNA extraction was performed using phenol - chloroform method which is the most common method for DNA extraction in order to achieve high quality of DNA was performed in the preparation of gene bank in this study. After relevant studies on this gene primers were designed and in use. After editing the sequences, nucleotide BLAST (Basic Local Alignment Search Tool) was performed using NCBI blast main page. The sequences obtained from each sample were aligned and corrected from any ambiguities and assembled using Bio edit program .Trees were generated using maximum parsimony (MP), a character-based algorithm and neighbor joining (NJ) a distance-based algorithm for phenetic analysis. The distance matrix option of MEGA4 was used to calculate genetic distance according to the Kimura 2-parameter model of sequence evolution. Based on the results obtained, the optical density of 260 to 280 nm in the samples was recorded between 1/8 - 2, indicating good quality DNA samples. Optimized PCR reaction to 16SrRNA gene amplification using the gradient between 48 - 60° C showed that the most suitable criteria for binding primers, 54 to 58 Celsius degrees respectively. The project objectives including the identification of the genetic structure of the species, and draw the phylogenetic trees using two genes 16SrRNA, making identification and registration of specified computer storage and regulate the structure and management of mentioned species by focus on genetic resources 10 species of majour commercial and noncommercial fishes in the Persia Gulf and Oman Sea through the creation of an integrated network of aquatic genetic resources in the region to try to identify genetic resources and aquatic gene bank. This type of analysis could be considered as an important tool to be used in broodstock selection in breeding programs. Also identify the species of fish named as Pseudorhombus pentophthalmus with a new haplotype and genetic differences 7% in compare to the samples recorded in the World gene Bank can considered as a major achievements of this research.
    Keywords: Biology ; Iran ; Persian Gulf ; Gene Bank ; mtDNA ; Fish ; Marine ; 16S rRNA ; Dasyatis bennetti ; Netuma thalassina ; Netuma bilineata ; Carcharhinus leucas ; Choerodon robustus ; Pseudorhombus pentophthalmus ; Pseudorhombus arsius ; Thunnus tonggol ; Euthynnus affinis ; Trichiurus lepturus ; Species
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 66
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  • 4
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    Unknown
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25369 | 18721 | 2018-09-14 07:50:12 | 25369 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The project was carried out between June of 2011 and November of 2012,8 laboratories of research center in Anzali (Plankton, Algae, Hydrochemistry, Physiology, Ichthyology, Bentose, Parazitology, Virology) and 7 laboratories of research center in Ahvaz (Clinical pathology, Plankton, Hydrochemistry, Physiology, Ichthyology, Bentose, Parazitology, Virology) were selected for accreditation. The main stages for establishment of the system consisted of: 1-Conducting a gap analysis to compare the present state of the laboratories with ISO/IEC 17025 2-Training General requirements for the competence of testing and calibration laboratories Validation of methods Estimation of uncertainty Internal audits 3- Performing of technical and management requirements 4-Submit of quality manual to ASCB center in England in order to accredit In August of 2012 The main results were including: 1-Increase the accuracy of measurement in laboratories 2-Improvement of the Repeatability and Reproducibility of the test methods 3-Traceability and standardization of test methods 4- Calibration of measurement instruments 6- Updating of test methods 7-Standardization of physical condition of the laboratories 8- Getting the certification from ASCB center in
    Keywords: Information Management ; Iran ; Standardization ; ISO/IEC 17025 ; Laboratory ; Accreditation ; Calibration ; National Inland water Aquaculture Institute ; South Iran Aquaculture research Institute
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 84
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  • 5
    Publication Date: 2021-05-19
    Description: The project was carried out between June of 2011 and November of 2012,8 laboratories of research center in Anzali (Plankton , Algae, Hydrochemistry , Physiology, Ichthyology, Bentose, Parazitology, Virology) and 7 laboratories of research center in Ahvaz (Clinical pathology, Plankton , Hydrochemistry , Physiology, Ichthyology, Bentose, Parazitology, Virology) were selected for accreditation. The main stages for establishment of the system consisted of: 1-Conducting a gap analysis to compare the present state of the laboratories with ISO/IEC 17025 2-Training General requirements for the competence of testing and calibration laboratories Validation of methods Estimation of uncertainty Internal audits 3- Performing of technical and management requirements 4-Submit of quality manual to ASCB center in England in order to accredit In August of 2012 The main results were including: 1-Increase the accuracy of measurement in laboratories 2-Improvement of the Repeatability and Reproducibility of the test methods 3-Traceability and standardization of test methods 4- Calibration of measurement instruments 6- Updating of test methods 7-Standardization of physical condition of the laboratories 8- Getting the certification from ASCB center in
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Standardization ; ISO/IEC 17025 ; Laboratory ; Accreditation ; Calibration ; National Inland water Aquaculture Institute ; South Iran Aquaculture research Institute
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 84pp.
    Location Call Number Expected Availability
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  • 6
    Publication Date: 2021-05-19
    Description: Genetic studies and gene banks preparation can identify guidelines for improving diversity and population structure and estimation, poaching and also the amount of cross breeding and provide genetic classification. In this study, sampling was performed from the important studied species habitats areas for the species such as: Dasyatis bennetti ، Netuma thalassina, Netuma bilineata Carcharhinus leucas ،Choerodon robustus ، Pseudorhombus pentophthalmus ،Pseudorhombus arsius Thunnus tonggol ،Euthynnus affinis and Trichiurus lepturus. Total DNA extraction was performed using phenol - chloroform method which is the most common method for DNA extraction in order to achieve high quality of DNA was performed in the preparation of gene bank in this study. After relevant studies on this gene primers were designed and in use. After editing the sequences, nucleotide BLAST (Basic Local Alignment Search Tool) was performed using NCBI blast main page.The sequences obtained from each sample were aligned and corrected from any ambiguities and assembled using Bio edit program .Trees were generated using maximum parsimony (MP), a character-based algorithm and neighbor joining (NJ) a distance-based algorithm for phenetic analysis. The distance matrix option of MEGA4 was used to calculate genetic distance according to the Kimura 2-parameter model of sequence evolution. Based on the results obtained, the optical density of 260 to 280 nm in the samples was recorded between 1/8 - 2, indicating good quality DNA samples. Optimized PCR reaction to 16SrRNA gene amplification using the gradient between 48 - 60° C showed that the most suitable criteria for binding primers, 54 to 58 Celsius degrees respectively.The project objectives including the identification of the genetic structure of the species, and draw the phylogenetic trees using two genes 16SrRNA, making identification and registration of specified computer storage and regulate the structure and management of mentioned species by focus on genetic resources 10 species of majour commercial and noncommercial fishes in the Persia Gulf and Oman Sea through the creation of an integrated network of aquatic genetic resources in the region to try to identify genetic resources and aquatic gene bank.This type of analysis could be considered as an important tool to be used in broodstock selection in breeding programs. Also identify the species of fish named as Pseudorhombus pentophthalmus with a new haplotype and genetic differences 7% in compare to the samples recorded in the World gene Bank can considered as a major achievements of this research.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Gene Bank ; mtDNA ; Fish ; Marine ; 16S rRNA ; Dasyatis bennetti ; Netuma thalassina ; Netuma bilineata ; Carcharhinus leucas ; Choerodon robustus ; Pseudorhombus pentophthalmus ; Pseudorhombus arsius ; Thunnus tonggol ; Euthynnus affinis ; Trichiurus lepturus ; Species
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 66pp.
    Location Call Number Expected Availability
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