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  • Inorganic Chemistry  (4)
  • β-glucuronidase  (3)
  • Life and Medical Sciences  (2)
  • Structured Biological Modelling  (2)
  • 72.55.+S  (1)
  • ATPase  (1)
  • Cell & Developmental Biology  (1)
  • Class II patatin gene  (1)
Collection
Keywords
Publisher
Years
  • 1
    Electronic Resource
    Electronic Resource
    Structured biological modelling: a method for the analysis and simulation of biological systems applied to oscillatory intracellular calcium waves
    Amsterdam : Elsevier
    Biosystems 27 (1992), S. 145-169 
    Details
    ISSN: 0303-2647
    Keywords: Oscillatory calcium waves ; Signal transduction ; Stochastic simulation ; Structured Biological Modelling ; Systems analysis
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0303-2647(92)90070-F
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  • 2
    Electronic Resource
    Electronic Resource
    Systems analysis in cell biology: From the phenomenological description towards a computer model of the intracellular signal transduction network (1993)
    Springer
    Cellular and molecular life sciences 49 (1993), S. 245-257 
    Details
    ISSN: 1420-9071
    Keywords: Structured analysis ; mathematical modelling ; stochastic simulation ; cell cycle control ; tumor biology ; calcium oscillations ; signal transduction ; Structured Biological Modelling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract In this paper we introduce a systematic approach for the modelling of complex biological systems which is especially useful for the analysis of signal transduction mechanisms in cell biology. It is shown that systems analysis in form of top-down levelled dataflow diagrams provides a powerful tool for the mathematical modelling of the system in terms of a stochastic formulation. Due to the exact formulation, the consistency of the model with the experimental results can be tested by means of a computer simulation. The method termed Structured Biological Modelling (SBM) is illustrated by modelling some aspects of the second messenger network which regulates cell proliferation. As an example for the strainghtforward development of a mathematical description a stochastic computer model for intracellular Ca2+ oscillations is presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01923534
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  • 3
    Electronic Resource
    Electronic Resource
    Presence of a transposon-like element in the promoter region of an inactive patatin gene in Solanum tuberosum L. (1990)
    Springer
    Plant molecular biology 14 (1990), S. 239-247 
    Details
    ISSN: 1573-5028
    Keywords: patatin ; potato ; transposon ; gene inactivation ; β-glucuronidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The promoter of the PGT3 patatin gene belonging to the class II subfamily is highly homologous to other class II patatin genes except for a 736 bp insertion in front of the putative transcription start site. The insertion is characterized by structural features resembling a transposable element such as an 11 bp inverted repeat at the termini and an 8 bp duplication flanking the insertion site. Despite the high homology to active patatin genes, fusion of its promoter to the β-glucuronidase reporter gene does not lead to detectable β-glucuronidase (GUS) activity in transgenic potato or tobacco plants, suggesting that the inactivation of this gene might be caused by the insertion of the transposon like element.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00018564
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  • 4
    Electronic Resource
    Electronic Resource
    A detailed study of the regulation and evolution of the two classes of patatin genes in Solanum tuberosum L. (1991)
    Springer
    Plant molecular biology 17 (1991), S. 1139-1154 
    Details
    ISSN: 1573-5028
    Keywords: patatin ; promoter ; β-glucuronidase ; potato
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The class-specific expression of patatin genes was investigated by analysing four new patatin genes. A class I patatin gene from cv. Berolina as well as a class I and two class II patatin genes from the monohaploid cultivar AM 80/5793 were isolated and partially sequenced. Sequence comparison indicates rearrangements as the major source for the generation of diversity between the different members of the classes. The expression of single genes was studied in potato plants transformed with chimaeric genes where the putative patatin promoters were fused to the GUS reporter gene. A detailed histochemical analysis reveals that both class I genes are expressed as the previously described class I patatin gene B33 from cv. Berolina [1], i.e. in the starch-containing cells of potato tubers and in sucrose-induced leaves. The class II gene pgT12 shows the same pattern as the previously described class II gene pgT2 [2], i.e. expression in root tips and in the vascular tissue of tubers, whereas no activity was detectable for pgT4. Thus the expression pattern of both classes of genes seems to be stable at least within or even between different cultivars.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00028731
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  • 5
    Electronic Resource
    Electronic Resource
    Isolation and characterization of P-type H+-ATPase genes from potato (1994)
    Springer
    Plant molecular biology 26 (1994), S. 979-988 
    Details
    ISSN: 1573-5028
    Keywords: ATPase ; phloem loading ; plasma membrane ; Solanum tuberosum L. ; multigene family ; sucrose transporter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract H+-ATPase cDNAs were identified in a potato leaf library using an Arabidopsis gene as a probe. Based on their sequences, the clones could be grouped into at least two classes. A similar classification was obtained from the analysis of sequence data from four tobacco genes. Both potato genes are expressed in all tissues analysed, higher levels of expression were found in leaves and stem than in roots and tubers. For both genes, no significant differences in level of expression could be detected under a variety of conditions such as cold treatment, anaerobiosis, sucrose induction or treatment with a synthetic cytokinin. Only 2,4-D and prolonged periods of darkness lead to a slight reduction in mRNA levels. The reduction in darkness was compensated after transfer of the plants back into the light. Expression of the ATPase genes remained constant in transgenic plants which are inhibited in phloem loading due to antisense inhibition of the sucrose transporter. On the other hand, expression of the sucrose transporter is inducible by auxin and cytokinin but not by sucrose. Taken together, these data suggest that at least the two plasma membrane H+-ATPase genes analysed are rather constant in their expression and that either other genes respond to external stimuli or that most of the regulation occurs at the post-transcriptional level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00028864
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  • 6
    Electronic Resource
    Electronic Resource
    A class II patatin promoter is under developmental control in both transgenic potato and tobacco plants (1989)
    Springer
    Molecular genetics and genomics 219 (1989), S. 390-396 
    Details
    ISSN: 1617-4623
    Keywords: Class II patatin gene ; β-glucuronidase ; Transgenic potato ; Transgenic tobacco ; Root tip
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A new member of the patatin gene family belonging to the class II subfamily was isolated and characterized by DNA sequencing. In order to study the expression profile of this gene, the promoter was fused to the β-glucuronidase gene and transferred to potato and tobacco. Histochemical analysis revealed high expression in a few defined cells in potato tubers and in a specific layer of both potato and tobacco root tips. In contrast to the developmentally and metabolically regulated class I patatin gene B33 this gene was not inducible by elevated levels of sucrose. Expression of this chimaeric gene was also found in callus and suspension cultures of potato.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00259611
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  • 7
    Electronic Resource
    Electronic Resource
    Comment on the observation of magnetoacoustic quantum oscillations in UBe13 (1993)
    Springer
    The European physical journal 91 (1993), S. 135-137 
    Details
    ISSN: 1434-6036
    Keywords: 72.55.+S
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The origin of previously reported magnetic quantum oscillations in UBe13 is possibly due to Al inclusions. New measurements of the a.c. susceptibility show Al inclusions which result from the method of crystal growth using aluminium as flux. We discuss the distribution and form of the inclusions and compare our dHvA measurements with those of the γ-orbits of Al.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01315226
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  • 8
    Electronic Resource
    Electronic Resource
    Fragmentierungsreaktionen an Carbonylverbindungen mit β-ständigen elektronegativen substituenten, XXXII: Reaktion von 2,2-Dialkyl-1-cyclohexyl-3-tosyloxy-1-propanon mit Nucleophilen (1976)
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 109 (1976), S. 2785-2792 
    Details
    ISSN: 0009-2940
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Fragmentation Reactions of Carbonyl Compounds with Electronegative β-Substituents, XXXII: Reaction of 2,2-Dialkyl-1-cyclohexyl-3-tosyloxy-1-propanone with NucleophilesTosylates 6 and 12 are synthesized starting from ketones 4 and 10,6 and 12 react with CH3Li to yield the oxetanes 17 and 20. LiBH4 reduction of 6 and 12 leads to the oxetanes 16 and 19, as well as to the tosyloxy alcohols 21 and 22. The neopentyl substitution products 7 and 13 are obtained from 6 and 12 with KCN in DMSO, whereas from 6 the cyano oxetane 18 was isolated too. With potassium tert-butoxide 6 reacts to give the enol ether 23, whereas 12 leads to the cyclo-butanone 25. The triflate 14 react with NaOCH3 to give the substitution product 15.
    Notes: Ausgehend von den Ketonen 4 und 10 werden die Tosylate 6 und 12 dargestellt, die mit CH3Li zu den Oxetanen 17 bzw. 20 reagieren. LiBH4-Reduktion von 6 bzw. 12 führt zu den Oxetanen 16 bzw. 19. daneben werden die Tosyloxy-alkohole 21 bzw. 22 isoliert. setzt man 6 bzw. 12 mit KCN in DMSO um, so werden die Neopentylsubstitutionsprodukte 7 bzw. 13, daneben aus 6 auch das Cyan-oxetan 18 erhalten. Mit Kalium-tert-butylalkoholat erhält man aus 6 den Enol-äther 23, dagegen aus 12 das Cyclobutanon 25. Das Triflat 14 reagiert mit NaOCH3 unter Substitution zu 15.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cber.19761090813
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  • 9
    Electronic Resource
    Electronic Resource
    Immunological approach to the characterization of the outer acrosomal membrane of boar spermatozoa (1985)
    New York, NY : Wiley-Blackwell
    Gamete Research 11 (1985), S. 143-155 
    Details
    ISSN: 0148-7280
    Keywords: spermatozoa ; acrosomal membrane ; membrane antigens ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Antiserum to purified boar spermatozoan outer acrosomal membrane (OAM) was raised in rabbits and adsorbed with boar liver and serum glutaraldehyde cross-linked immunoadsorbents. The IgG fraction of the antiserum was purified by (NH4)2SO4 precipitation followed by ion-exchange chromatography. Indirect immunofluorescence showed bright fluorescent staining of the acrosomal cap of boar spermatozoa and to a lesser extent of the acrosomes of bull and goat spermatozoa after incubation with anti-OAM-IgG. Immuno-electron microscopy further confirmed the specificity of the antibody for the OAM. Preincubation of the anti-OAM-IgG with isolated OAM, completely abolished its reactivity. When tested by ELISA, anti-OAM-IgG reacted with boar, bull, goat, and human spermatozoa; however, its binding activity to boar spermatozoa was significantly greater as compared to spermatozoa from the other species tested.In an effort to identify OAM antigens recognized by this antiserum, the isolated boar OAM was labeled either with 3H or with 125I and solubilized by mild detergent treatment. The extracted components were immunoprecipitated with anti-OAM-IgG and protein A-bearing S. aureus and the thus isolated antigens were analysed on SDS-PAGE. The results suggest that anti-OAM-IgG recognized one high molecular 3H-labeled glycoprotein (270 kd), and four 125I-labeled polypeptides of lower molecular weight of the boar OAM.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120110205
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  • 10
    Electronic Resource
    Electronic Resource
    Meßverfahren im Chemie- und Biologieunterricht. Von Wolfgang Jungbauer. Praxis Schriftenreihe Chemie, Hrsg. von Wolfgang Glöckner. Band 34. 107 S. Aulis Verlag Deubner & Co. Köln, 1980. DM 16,80 (1981)
    Weinheim : Wiley-Blackwell
    Biologie in unserer Zeit 11 (1981), S. 128-128 
    Details
    ISSN: 0045-205X
    Keywords: Life and Medical Sciences
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/biuz.19810110412
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