ISSN:
1573-5028
Keywords:
gene regulation
;
nodule
;
nodulin
;
Phaseolus vulgaris
;
transgenic plants
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract In Phaseolus vulgaris there is a nodulin family, Npv30, of ca. 30 kDa, as detected in an in vitro translation assay [2]. We isolated a gene (npv30-1) for one of the members of this family. The nucleotide sequence of the promoter of npv30-1 contains nodule-specific motifs common to other late nodulin genes. The promoter was fused to the GUS reporter gene; this chimeric fusion was introduced into Lotus corniculatus via Agrobacterium rhizogenes transformation. GUS activity was only detected in the infected cells of the nodules of transgenic plants. By contrast, the expression of a 35S-GUS construct was restricted to the uninfected cells and the vascular tissue.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00019510
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