Publikationsdatum:
2009-10-10
Beschreibung:
We describe Hi-C, a method that probes the three-dimensional architecture of whole genomes by coupling proximity-based ligation with massively parallel sequencing. We constructed spatial proximity maps of the human genome with Hi-C at a resolution of 1 megabase. These maps confirm the presence of chromosome territories and the spatial proximity of small, gene-rich chromosomes. We identified an additional level of genome organization that is characterized by the spatial segregation of open and closed chromatin to form two genome-wide compartments. At the megabase scale, the chromatin conformation is consistent with a fractal globule, a knot-free, polymer conformation that enables maximally dense packing while preserving the ability to easily fold and unfold any genomic locus. The fractal globule is distinct from the more commonly used globular equilibrium model. Our results demonstrate the power of Hi-C to map the dynamic conformations of whole genomes.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2858594/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2858594/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lieberman-Aiden, Erez -- van Berkum, Nynke L -- Williams, Louise -- Imakaev, Maxim -- Ragoczy, Tobias -- Telling, Agnes -- Amit, Ido -- Lajoie, Bryan R -- Sabo, Peter J -- Dorschner, Michael O -- Sandstrom, Richard -- Bernstein, Bradley -- Bender, M A -- Groudine, Mark -- Gnirke, Andreas -- Stamatoyannopoulos, John -- Mirny, Leonid A -- Lander, Eric S -- Dekker, Job -- HG003143/HG/NHGRI NIH HHS/ -- R01 HG003143/HG/NHGRI NIH HHS/ -- R01 HG003143-06/HG/NHGRI NIH HHS/ -- R01HL06544/HL/NHLBI NIH HHS/ -- R37DK44746/DK/NIDDK NIH HHS/ -- T32 HG002295/HG/NHGRI NIH HHS/ -- U54HG004592/HG/NHGRI NIH HHS/ -- New York, N.Y. -- Science. 2009 Oct 9;326(5950):289-93. doi: 10.1126/science.1181369.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Broad Institute of Harvard and Massachusetts Institute of Technology (MIT), MA 02139, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19815776" target="_blank"〉PubMed〈/a〉
Schlagwort(e):
Biotin
;
Cell Line, Transformed
;
Cell Nucleus/*ultrastructure
;
Chromatin/*chemistry
;
Chromatin Immunoprecipitation
;
*Chromosomes, Human/chemistry/ultrastructure
;
Computational Biology
;
DNA/*chemistry
;
Gene Library
;
*Genome, Human
;
Humans
;
In Situ Hybridization, Fluorescence
;
Models, Molecular
;
Monte Carlo Method
;
Nucleic Acid Conformation
;
Principal Component Analysis
;
Protein Conformation
;
Sequence Analysis, DNA
Print ISSN:
0036-8075
Digitale ISSN:
1095-9203
Thema:
Biologie
,
Chemie und Pharmazie
,
Informatik
,
Medizin
,
Allgemeine Naturwissenschaft
,
Physik
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