Publication Date:
2002-03-23
Description:
Full-length complementary DNAs (cDNAs) are essential for the correct annotation of genomic sequences and for the functional analysis of genes and their products. We isolated 155,144 RIKEN Arabidopsis full-length (RAFL) cDNA clones. The 3'-end expressed sequence tags (ESTs) of 155,144 RAFL cDNAs were clustered into 14,668 nonredundant cDNA groups, about 60% of predicted genes. We also obtained 5' ESTs from 14,034 nonredundant cDNA groups and constructed a promoter database. The sequence database of the RAFL cDNAs is useful for promoter analysis and correct annotation of predicted transcription units and gene products. Furthermore, the full-length cDNAs are useful resources for analyses of the expression profiles, functions, and structures of plant proteins.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Seki, Motoaki -- Narusaka, Mari -- Kamiya, Asako -- Ishida, Junko -- Satou, Masakazu -- Sakurai, Tetsuya -- Nakajima, Maiko -- Enju, Akiko -- Akiyama, Kenji -- Oono, Youko -- Muramatsu, Masami -- Hayashizaki, Yoshihide -- Kawai, Jun -- Carninci, Piero -- Itoh, Masayoshi -- Ishii, Yoshiyuki -- Arakawa, Takahiro -- Shibata, Kazuhiro -- Shinagawa, Akira -- Shinozaki, Kazuo -- New York, N.Y. -- Science. 2002 Apr 5;296(5565):141-5. Epub 2002 Mar 21.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center (GSC), 3-1-1 Koyadai, Tsukuba 305-0074, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11910074" target="_blank"〉PubMed〈/a〉
Keywords:
Arabidopsis/*genetics/metabolism
;
Arabidopsis Proteins/genetics/metabolism
;
Chromosome Mapping
;
Cloning, Molecular
;
Computational Biology
;
*DNA, Complementary/isolation & purification
;
DNA, Plant/genetics
;
Databases, Nucleic Acid
;
*Expressed Sequence Tags
;
Gene Expression
;
Gene Library
;
*Genes, Plant
;
Genome, Plant
;
Promoter Regions, Genetic
;
RNA, Messenger/genetics
;
Sequence Analysis, DNA
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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