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  • (3H) Tetracycline purification  (1)
  • AMO  (1)
  • Escherichia coli  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 141 (1985), S. 260-265 
    ISSN: 1432-072X
    Keywords: (3H) Tetracycline purification ; Initial uptake kinetics ; Monophasic tetracycline uptake ; Tetracycline binding ; Temperature effect on tetracycline uptake ; Escherichia coli
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Experiments measuring the initial uptake of commercial (3H) tetracycline exhibit two distinct kinetic phases: a rapid phase followed by a slow phase. (3H) tetracycline purified by chromatography on a Dowex 50WX2 column exhibited only monophasic rapid uptake when tested with susceptible Escherichia coli cells. Cyanide inhibited the uptake of purified (3H) tetracycline only partially while transport of proline and maltose was entirely abolished. Energy independent accumulation of tetracycline may be accounted for by binding to cellular constituents. Uptake of tetracycline-as measured by inhibition of β-galactosidase synthesis-was strongly affected by a shift in temperature from 37°C to 21°C while carrier-mediated transport systems revealed only minor reductions. Taken together with the non-saturability of tetracycline uptake and the evidence for diffusion of tetracycline through phospholipid bilayers [Argast and Beck (1984) Antimicrob Agents Chemother 26:263–265] these data support the hypothesis that tetracycline enters the cytoplasm by diffusion.
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 2021-07-03
    Description: The Southern Annular Mode (SAM) is the predominant atmospheric variability mode in the Southern Hemisphere. In this paper, we present the spatial variability results of the SAM pattern for the period 1979–2018. The SAM‐intrinsic pattern variability analysis is based on the principal component analysis (PCA), which is carried out for the ERA‐Interim 500 hPa geopotential height (GPH) data set. A spatiotemporally resolved data set of SAM pattern maps (PCA loadings) is derived by projecting monthly shifted sub‐sequences of SAM index values (PCA scores) on the corresponding GPH anomalies. The dominant SAM structure within single pattern fields is mapped automatically and can be interpreted as the Southern Hemisphere polar front. This data set allows an analysis of the geographical positions of the characteristic circumpolar SAM structure over four decades and shows considerable variability over space and time. Five different states of SAM patterns, which are associated with characteristic circulation anomalies during different phases of the study period, are identified. Station‐based Antarctic temperature anomalies can be synoptically explained by these circulation anomalies. The overall latitudinal trend of the SAM pattern indicates an intensification of the meridional structure, especially over the East Antarctic Southern Ocean. Furthermore, we show that the SAM pattern variability is significantly correlated with the Pacific Decadal Oscillation and the Atlantic Multidecadal Oscillation. Composites of 500 hPa GPH anomalies during the positive and negative phases of the respective indices indicate teleconnections with Pacific Decadal Oscillation and Atlantic Multidecadal Oscillation, and this can explain latitudinal trends of the SAM pattern.
    Description: Key Points: We present a new approach to examine the spatiotemporal Southern Annular Mode pattern variability. Station‐based Antarctic temperature anomalies are related to different structures of the Southern Annular Mode. The trend pattern shows an increasing meridional structure and correlations with Pacific and Atlantic multidecadal oscillations.
    Description: DLR Management Board: Young Investigator Group Leader Program
    Keywords: 551.5 ; Southern Annular Mode ; SAM pattern variability ; Antarctic circulation variability ; Antarctic station temperature ; PDO ; AMO
    Type: article
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