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  • Aspergillosis  (2)
  • Genetic diversity  (2)
  • Life Sciences (General)
  • Polymer and Materials Science
  • Springer  (4)
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Keywords
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  • 1
    Electronic Resource
    Electronic Resource
    Sequential pathological studies in the udder of goats intramammarily infected withAspergillus fumigatus (1994)
    Springer
    Mycopathologia 126 (1994), S. 9-14 
    Details
    ISSN: 1573-0832
    Keywords: Aspergillosis ; Goats ; Pathology ; Udder
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Intramammary inoculation of goats withAspergillus fumigatus spores resulted in the development of mastitis with characteristic gross and microscopic lesions. The mastitis and the lesions were restricted to the infected udder halves only and there was no dissemination of infection to other tissues of the body. The experiment was continued for 45 days. Gross changes in the infected udder were observed up to the 45th day post-infection. The lesions, in general, included variable sized abscesses in the first 15 days followed by development of varying sized greyish-white nodules in the infected udders. Microscopic changes consisted of granulomatous reaction with well developed granulomas in the infected udders. Hyphae and spores ofAspergillus fumigatus could be demonstrated in sections of the infected udders up to 45 days after infection. Reisolation of the fungus consistently was achieved up to 45 days. It is concluded that intramammary inoculation ofAspergillus fumigatus spores in goats leads to chronic granulomatous mastitis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01371167
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  • 2
    Electronic Resource
    Electronic Resource
    Sequential pathological studies in goats infected intratracheally withAspergillus fumigatus (1993)
    Springer
    Mycopathologia 121 (1993), S. 77-81 
    Details
    ISSN: 1573-0832
    Keywords: Aspergillosis ; Goats ; Pathology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Intratracheal inoculation of goats withAspergillus fumigatus spores resulted in the development of characteristic gross and microscopic lesions. The lesions were restricted to lungs and there was no dissemination of infection to other tissues of the body except liver in one goat 16 days after infection. The experiment was continued for 37 days. Gross changes in lungs were observed up to the 24th day post-infection. The lesions, in general, included congestion and oedema in the first 6 days followed by the development of varying greyish-white nodules in the lungs. Microscopic changes consisted of granulomatous reaction with well developed granulomas in lungs. Hyphae and conidiophores with fruiting bodies ofAspergillus fumigatus could be demonstrated in sections up to 24 days of infection. Reisolation of the fungus consistently was achieved up to 24 days. It is concluded that intratracheal inoculation ofAspergillus fumigatus spores in goats leads to pulmonary aspergillosis up to 24 days.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01103574
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  • 3
    Electronic Resource
    Electronic Resource
    The use of microsatellites for detecting DNA polymorphism, genotype identification and genetic diversity in wheat (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 584-592 
    Details
    ISSN: 1432-2242
    Keywords: Key words Microsatellite markers ; Wheat ; Genetic diversity ; Genotype identification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A set of 20 wheat microsatellite markers was used with 55 elite wheat genotypes to examine their utility (1) in detecting DNA polymorphism, (2)in the identifying genotypes and (3) in estimating genetic diversity among wheat genotypes. The 55 elite genotypes of wheat used in this study originated in 29 countries representing six continents. A total of 155 alleles were detected at 21 loci using the above microsatellite primer pairs (only 1 primer amplified 2 loci; all other primers amplified 1 locus each). Of the 20 primers amplifying 21 loci, 17 primers and their corresponding 18 loci were assigned to 13 different chromosomes (6 chromosomes of the A genome, 5 chromosomes of the B genome and 2 chromosomes of the D genome). The number of alleles per locus ranged from 1 to 13, with an average of 7.4 alleles per locus. The values of average polymorphic information content (PIC) and the marker index (MI) for these markers were estimated to be 0.71 and 0.70, respectively. The (GT)n microsatellites were found to be the most polymorphic. The genetic similarity (GS) coefficient for all possible 1485 pairs of genotypes ranged from 0.05 to 0.88 with an average of 0.23. The dendrogram, prepared on the basis of similarity matrix using the UPGMA algorithm, delineated the above genotypes into two major clusters (I and II), each with two subclusters (Ia, Ib and IIa, IIb). One of these subclusters (Ib) consisted of a solitary genotype (E3111) from Portugal, so that it was unique and diverse with respect to all other genotypes belonging to cluster I and placed in subcluster Ia. Using a set of only 12 primer pairs, we were able to distinguish a maximum of 48 of the above 55 wheat genotypes. The results demonstrate the utility of microsatellite markers for detecting polymorphism leading to genotype identification and for estimating genetic diversity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050077
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  • 4
    Electronic Resource
    Electronic Resource
    Selection of diverse genotypes for heterosis in yield and response in toria (Brassica campestris L.) (1985)
    Springer
    Theoretical and applied genetics 69 (1985), S. 515-517 
    Details
    ISSN: 1432-2242
    Keywords: Toria ; Genetic diversity ; D2-correlation coefficient ; Heterosis ; Basic yield potential ; Brassica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Thirty-one genotypes of toria (Brassica campestris L.) were grown in twelve environments and subsequently analysed in order to select potential parents which expressed diversity for both 12 different characters (estimated by Mahalanobis' D2 technique) and response to the environments (estimated on the basis of negative correlation between deviations in seed yield of a pair of genotypes from their respective environmental means). Coefficients of determination (r2) were also used to measure the reliability of correlation, which is the basis of diversity of response. Stability parameters (b and S2d) and mean seed yield were also considered in selecting potential parents. On the basis of these criteria, three pairs of genotypes (ITSA and TCSU-1, TCSU-7 and TH-8 and Ludhiana Composite-1 and TH-4) are recommended to be used as parents for hybridization programmes so that heterosis both in seed yield and response may be exploited.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00251095
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