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  • Articles  (86)
  • Springer  (40)
  • Cell Press  (26)
  • Copernicus  (20)
  • Biology  (63)
  • Geosciences  (23)
  • Nature of Science, Research, Systems of Higher Education, Museum Science  (1)
Collection
  • Articles  (86)
Journal
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Journal of ornithology 120 (1979), S. 1-11 
    ISSN: 1439-0361
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Summary The population of the White Stork in Baden-Württemberg (South-west Germany) was approximately constant in the post-war years until 1959. Beginning with 1960 it started to decline with an average rate of 13% per year. After 1960 the average annual mortality rate increased both in adults and in first year birds. In addition the average age of first breeding and the average percentage of pairs without breeding success increased in the second period. The latter means a decrease of breeding success. Some possible causes for the changes of these parameters are mentioned.
    Notes: Zusammenfassung Der Bestand des Weißstorchs in Baden-Württemberg blieb in den Nachkriegsjahren bis 1959 annähernd gleich; erst seit 1960 nehmen die Bestandszahlen jährlich um durchschnittlich etwa 13% ab. Sowohl bei den Altvögeln als auch bei den einjährigen Störchen hat die durchschnittliche jährliche Verlustrate nach 1960 zugenommen. Ebenso erhöhte sich in der zweiten Periode das durchschnittliche Erstbrutalter. Nach 1960 nahm der durchschnittliche Anteil erfolglos brütender Paare zu, der durchschnittliche Bruterfolg verringerte sich. Einige der möglichen Ursachen für die Veränderungen der einzelnen Parameter werden angeführt.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A new approach is presented which allows the in vivo visualization of individual chromosome territories in the nuclei of living human cells. The fluorescent thymidine analog Cy3-AP3-dUTP was microinjected into the nuclei of cultured human cells, such as human diploid fibroblasts, HeLa cells and neuroblastoma cells. The fluorescent analog was incorporated during S-phase into the replicating genomic DNA. Labelled cells were further cultivated for several cell cycles in normal medium. This well-known scheme yielded sister chromatid labelling. Random segregation of labelled and unlabelled chromatids into daughter nuclei resulted in nuclei exhibiting individual in vivo detectable chromatid territories. The territories were composed of subcompartments with diameters ranging between approximately 400 and 800 nm which we refer to as subchromosomal foci. Time-resolved in vivo studies demonstrated changes of positioning and shape of territories and subchromosomal foci. The hypothesis that subchromosomal foci persist as functionally distinct entities was supported by double labelling of chromatin with CldU and IdU, respectively, at early and late S-phase and subsequent cultivation of corresponding cells for 5–10 cell cycles before fixation and immunocytochemical detection. This scheme yielded segregated chromatid territories with distinctly separated subchromosomal foci composed of either early- or late-replicating chromatin. The size range of subchromosomal foci was similar after shorter (2 h) and longer (16 h) labelling periods and was observed in nuclei of both living and fixed cells, suggesting their structural identity. A possible functional relevance of chromosome territory compartmentalization into subchromosomal foci is discussed in the context of present models of interphase chromosome and nuclear architecture.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Immunogenetics 6 (1978), S. 269-276 
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Immunofluorescence tests indicate that alloantibodies specific for mouse histocompatibility antigens H-1a, H-3a, and H-13a have been produced, using four different immunizations. Furthermore, an immunization employing donors and recipients which were H-2k at the MHC produced stronger anti-H-3a and anti-H-13a than did immunizations where donors and recipients were H-2b at the MHC.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Immunogenetics 4 (1977), S. 257-266 
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Alloantibodies specific for non-H-2 histocompatibility antigens of the mouse have been produced. Immunization (BALB/cJ×DBA/2J)F1 anti-B10.D2/n was conducted, followed by hemagglutination, immunofluorescence, and mixed hemabsorption tests on absorbed and unabsorbed sera. The results indicate that antibodies specific for H-3a and H-8a antigens are present. In addition, H-8a antigenic determinants were detected on erythrocyte membrane surfaces, as well as on cells of other body tissues.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-0770
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Computer Science , Physics
    Notes: Abstract An experiment is described which compares the performance of a neural network to human performance on a visual task which consists of detecting a target in a background image of correlated noise. A three-layer, feed-forward, multi-layer perceptron is trained to indicate the presence or absence of a target in images also presented to human observers. The basis for the comparison between the network and the human observers is the receiver operating characteristic (ROC) curve. Network performance is comparable to human performance for this particular task.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Biological cybernetics 59 (1988), S. 257-263 
    ISSN: 1432-0770
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Computer Science , Physics
    Notes: Abstract The utility of the back-propagation method in establishing suitable weights in a distributed adaptive network has been demonstrated repeatedly. Unfortunately, in many applications, the number of iterations required before convergence can be large. Modifications to the back-propagation algorithm described by Rumelhart et al. (1986) can greatly accelerate convergence. The modifications consist of three changes:1) instead of updating the network weights after each pattern is presented to the network, the network is updated only after the entire repertoire of patterns to be learned has been presented to the network, at which time the algebraic sums of all the weight changes are applied:2) instead of keeping η, the “learning rate” (i.e., the multiplier on the step size) constant, it is varied dynamically so that the algorithm utilizes a near-optimum η, as determined by the local optimization topography; and3) the momentum factor α is set to zero when, as signified by a failure of a step to reduce the total error, the information inherent in prior steps is more likely to be misleading than beneficial. Only after the network takes a useful step, i.e., one that reduces the total error, does α again assume a non-zero value. Considering the selection of weights in neural nets as a problem in classical nonlinear optimization theory, the rationale for algorithms seeking only those weights that produce the globally minimum error is reviewed and rejected.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Naturwissenschaften 68 (1981), S. 507-512 
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Notes: Abstract Triboluminescence is the light emitted when mechanical energy is applied to a crystal. Many common substances such as sugar and copper sulfate are triboluminescent. The origin of the light and the mechanical, chemical, and crystallographic requirements of the phenomenon are described in this paper.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Plant systematics and evolution 191 (1994), S. 131-146 
    ISSN: 1615-6110
    Keywords: Leguminosae ; Phaseolus ; Vigna ; Lectin genes ; phylogeny ; diversity ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Restriction fragment length polymorphisms at the phytohemagglutinin (PHA) locus were determined among 21 genotypes ofPhaseolus vulgaris, P. coccineus, P. acutifolius, P. lunatus, and threeVigna species, using five restriction enzymes and one double digestion, in order to provide molecular evidence for their genetic relatedness. The dissimilarity between genotypes was estimated from binary RFLP data. The dissimilarity was high among species (from 0.75 to 0.95), and of variable extent among genotypes of the same species (0.33–0.89). InP. vulgaris, two different DNA hybridization patterns were found, giving further evidence for two major gene pools in that species. The restriction patterns ofP. vulgaris var.aborigineus, the putative ancestral form ofP. vulgaris, exhibit clear homology toP. vulgaris genotypes. An undefined landrace from Taiwan could be identified as aP. vulgaris genotype. RFLP-based trees for the phytohemagglutinin genes of the species studied were computed with several distance matrix and parsimony methods.
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  • 9
    ISSN: 1573-6849
    Keywords: Cicer arietinum ; fluorescence in situ hybridization ; microsatellites ; telomeres
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We used fluorescence in situ hybridization to probe the physical organization of five simple sequence repeat motifs and the Arabidopsis-type telomeric repeat in metaphase chromosomes and interphase nuclei of chickpea (Cicer arietinum L.). Hybridization signals were observed with the whole set of probes and on all chromosomes, but the distribution and intensity of signals varied depending on the motif. On root-tip metaphase chromosomes, CA and GATA repeats were mainly restricted to centromeric areas, with additional GATA signals along some chromosomes. TA, A and AAC repeats were organized in a more dispersed manner, with centromeric regions being largely excluded. In interphase nuclei of the inner integument, CA and GATA signals predominantly occurred in the heterochromatic endochromocentres, whereas the other motifs were found both in eu- and heterochromatin. The distribution of the Arabidopsis-type telomeric repeat (TTTAGGG)n on metaphase chromosomes was found to be quite exceptional. One major cluster of repeats was spread along the short arm of chromosome B, whereas a second, weaker signal occurred interstitially on chromosome A. Only faint and inconsistent hybridization signals were visualized with the same probe at the chromosomal termini.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell, tissue and organ culture 1 (1981), S. 265-273 
    ISSN: 1573-5044
    Keywords: Ipomoea ; morning glory ; cell culture ; acid phosphatases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two acid phosphatases isolated from culturedIpomoea (moring glory) cells were separated by column chromatography on DEAE-cellulose. The two acid phosphatases have different pH optima (pH 4.8–5.0 and 6.0) and do not require the presence of divalent ions. The enzymes possess high activity toward pyrophosphate,p-nitrophenylphosphate, nucleoside di- and triphosphates, and much less activity toward nucleoside monophosphates and sugar esters. The two phosphatases differ from each other in Michaelis constants, in the degree of inhibition by arsenate, fluoride and phosphate and have quantitative differences of substrate specificity. In addition, they also differ in their response to various ions.
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