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  • 1
    Electronic Resource
    Electronic Resource
    Genetic localisation of transformation competence in diploid potato (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 557-562 
    Details
    ISSN: 1432-2242
    Keywords: Solanum tuberosum ; Phytophthora infestans ; Transposable element ; Transformation competence ; Linkage analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In the course of improving diploid potato genotypes for transformation ability, selection for specific components affecting regeneration and transformation was carried out. From a segregating population between two good regenerating clones a selection was made to yield an optimal well-transforming and fertile genotype J92-6400-A16. This plant yielded predominantly diploid transformants and was heterozygous for the gene R1, conferring resistance to Phytophthora infestans. The speed of, and competence for, regeneration and transformation on both sides of the stem explant were improved. A competence factor for tranformation was found to be linked with the R1 locus and a molecular marker on chromosome 5. The male fertility of transformants was frequently decreased to a great extent, whereas female fertility was not so markedly affected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223279
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  • 2
    Electronic Resource
    Electronic Resource
    Immunocytolocalization of glutamine synthetase in mesophyll and phloem of leaves ofSolanum tuberosum L. (1992)
    Springer
    Protoplasma 167 (1992), S. 66-73 
    Details
    ISSN: 1615-6102
    Keywords: Glutamine synthetase isoenzymes ; Ammonia assimilation ; Solanum tuberosum ; Gold immunolocalization ; Western tissue print ; Phloem transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Localization of glutamine synthetase inSolanum tuberosum leaves was investigated by techniques of Western tissue printing and immunogold electron microscopy. Anti-GS antibodies used in immunolocalization recognize two peptides (45 kDa and 42 kDa) on Western blots. Antibody stained tissue prints on nitrocellulose membranes allowed low resolution localization of GS. Immunostaining was most evident in the adaxial phloem of the leaf midribs and petiole veins. High-resolution localization of glutamine synthetase by immunogold electron microscopy revealed that this enzyme occurs in both the chloroplasts and the cytosol ofS. tuberosum leaf cells. However, GS was specifically associated with the chloroplasts of mesophyll cells and with the cytoplasm of phloem companion cells. The evidence for cell-specific localization of chloroplast and cytosolic GS presented here agrees with the recently reported cell-specific pattern of expression of GUS reporter gene, directed by promoters for chloroplast and cytosolic GS form in tobacco transgenic plants. These data provide additional clues to the interpretation of the functional role of these different isoenzymes and its relationship with their specific localization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01353582
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  • 3
    Electronic Resource
    Electronic Resource
    Segregation analysis and RFLP mapping of the R1 and R3 alleles conferring race-specific resistance to Phytophthora infestans in progeny of dihaploid potato parents (1994)
    Springer
    Molecular genetics and genomics 242 (1994), S. 749-754 
    Details
    ISSN: 1617-4623
    Keywords: Potato ; Solanum tuberosum ; Phytophthora infestans ; RFLP ; R genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phytophthora infestans (Mont.) de Bary is the most important fungal pathogen of the potato (Solanum tuberosum). The introduction of major genes for resistance from the wild species S. demissum into potato cultivars is the earliest example of breeding for resistance using wild germplasm in this crop. Eleven resistance alleles (R genes) are known, differing in the recognition of corresponding avirulence alleles of the fungus. The number of R loci, their positions on the genetic map and the allelic relationships between different R variants are not known, except that the R1 locus has been mapped to potato chromosome V The objective of this work was the further genetic analysis of different R alleles in potato. Tetraploid potato cultivars carrying R alleles were reduced to the diploid level by inducing haploid parthenogenetic development of 2n female gametes. Of the 157 isolated primary dihaploids, 7 set seeds and carried the resistance alleles R1, R3 and R10 either individually or in combinations. Independent segregation of the dominant R1 and R3 alleles was demonstrated in two F1 populations of crosses among a dihaploid clone carrying R1 plus R3 and susceptible pollinators. Distorted segregation in favour of susceptibility was found for the R3 allele in 15 of 18 F1 populations analysed, whereas the RI allele segregated with a 1:1 ratio as expected in five F1 populations. The mode of inheritance of the R10 allele could not be deduced as only very few F1 hybrids bearing R10 were obtained. Linkage analysis in two F1 populations between R1, R3 and RFLP markers of known position on the potato RFLP maps confirmed the position of the R1 locus on chromosome V and localized the second locus, R3, to a distal position on chromdsome XI.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00283432
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  • 4
    Electronic Resource
    Electronic Resource
    Race specific resistance against Phytophthora infestans in potato is controlled by more genetic factors than only R-genes (1996)
    Springer
    Euphytica 90 (1996), S. 331-336 
    Details
    ISSN: 1573-5060
    Keywords: potato ; Solanum tuberosum ; Phytophthora infestans ; R-genes ; suppressor ; late blight
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary For RFLP mapping of R-genes, determining resistance to specific races of Phytophthora infestans in tetraploid potato, it is necessary to develop well segregating populations at the 2x level. During mapping studies, evidence was obtained that more genetic factor(s) are involved in the expression of R-genes than conventionally believed. Two experiments are described in which such an additional genetic factor was suppressing or enhancing the expression of unknown R nand R ifactors. R nand R iappeared to be present in the investigated plant material, containing R4 and R10, or in one of the susceptible crossing parents. In a third experiment, the expression and the segregation of the well known R1 gene was influenced by an additional genetic factor. In that case there were indications for a dominant suppressor. This was established by the selection of susceptible plants carrying a RFLP allele of probe GP21 closely linked to R1. In three of the four F1 populations, resulting from crosses between such susceptible plants and susceptible tester plants, resistnat progenies were found. The resistance appeared to be R1-specific. This clearly indicates that in three of the four investigated susceptible plants, the R1 gene was still present but not expressed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00027484
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  • 5
    Electronic Resource
    Electronic Resource
    Gibberellic acid and the inhibition of aerial tuberisation in Solanum tuberosum L. (1984)
    Springer
    Plant growth regulation 2 (1984), S. 41-47 
    Details
    ISSN: 1573-5087
    Keywords: Solanum tuberosum ; tuberisation ; gibberellic acid ; starch accumulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The sensitivity of aerial and subterranean tuberisation to photoperiod was studied in potato (Solanum tuberosum cv. Aracy). Although photoperiodic sensitivity varied with the position along the stem, all buds could be induced to develop tubers under SD. Gibberellic acid (GA3) applied to induced (30 short days) cuttings inhibited the photoperiodic effect. No tubers were formed and orthotropic shoots developed instead. The GA3 caused a reduction in starch content in induced buds, lowering it to the same level as found in long-day treated plants. However, α-amylase activity of buds of induced plants was not affected by GA3, suggesting that GA3 does not inhibit tuberisation by promotion of starch hydrolysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00024094
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  • 6
    Electronic Resource
    Electronic Resource
    Genetic advance for chip colour in potatoes (1995)
    Springer
    Euphytica 84 (1995), S. 133-138 
    Details
    ISSN: 1573-5060
    Keywords: breeding ; chip colour ; potato ; processing ; selection response ; Solanum tuberosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The objective of this study was to investigate the response to selection for chip colour after harvest (CH), storage at 12.8°C (CR) and at 3°C (CC) in three hybrid populations. Population 1 was derived from crossing ND860-2 (cold chipper) with F58089 (regular chipper), Population 2 was obtained from crossing ND860-2 with Russette (nonchipper), and Population 3 was derived from crossing Russette with F58089. Eighty-five to ninety-six random clones for each population plus ten check cultivars were planted in 1991 at two locations in East Canada. For CH, Population 3 had the highest predicted gain. No genetic variation for this trait was detected in Population 1. The potential genetic advance by selection within Population 1, as measured by the predicted mean of the selected clones, however, was similar to the others because Population 1 has a higher mean. For CR, Populations 2 and 3 had similar expected response estimates. Population 1 had no genetic variation for CR but showed similar potential advance to Population 2 and higher than Population 3. For CC, Population 2 had the highest predicted gain. The predicted means of selected clones of Populations 1 and 2 were higher than that of Population 3. Consequently, Populations 1 and 2 had greater potential for improvement for CC than Population 3.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01677951
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