Summary
For RFLP mapping of R-genes, determining resistance to specific races of Phytophthora infestans in tetraploid potato, it is necessary to develop well segregating populations at the 2x level. During mapping studies, evidence was obtained that more genetic factor(s) are involved in the expression of R-genes than conventionally believed. Two experiments are described in which such an additional genetic factor was suppressing or enhancing the expression of unknown R nand R ifactors. R nand R iappeared to be present in the investigated plant material, containing R4 and R10, or in one of the susceptible crossing parents. In a third experiment, the expression and the segregation of the well known R1 gene was influenced by an additional genetic factor. In that case there were indications for a dominant suppressor. This was established by the selection of susceptible plants carrying a RFLP allele of probe GP21 closely linked to R1. In three of the four F1 populations, resulting from crosses between such susceptible plants and susceptible tester plants, resistnat progenies were found. The resistance appeared to be R1-specific. This clearly indicates that in three of the four investigated susceptible plants, the R1 gene was still present but not expressed.
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Anonymus, 1991. 66eBeschrijvende Rassenlijst voor Landbouwgewassen 1991. Publisher Leiter-Nypels b.v. Maastricht, The Netherlands. pp. 336.
Bartos P., 1993. Chromosome 1R of rye in wheat breeding. Plant Breeding Abst. 63: 1203–1211.
Black W., 1945. Inheritance of resistance to blight (Phytophthora infestans) in potato: unbalanced segragations. Proc Royal Soc Edinburgh, Section B. 62: 171–181.
Black W., C. Mastenbroek, W.R. Mills & L.C. Peterson, 1953. A proposal for an international nomenclature of races of Phytophthora infestans and of genes controlling immunity in Solanum demissum derivates. Euphytica 2: 173–240.
Colon, L.T., R.C. Jansen & D.J. Budding, 1995. Partial resistance to late blight (Phytophthora infestans) in hybrid progenies of four South American Solanum species crossed with diploid S. tuberosum. Theor Appl Genet (in press).
Dellaporta S.L., J. Woods & J.B. Hichs, 1983. A plant DNA minipreparation version II. Plant Mol Biol Rep 1: 19–21.
De Maine M.J., 1978. Field resistance to late blight and potato root eelworm in group tuberosum dihaploids. Euphytica 27: 305–315.
El-Kharbotly A., C. Leonards-Schippers, D.J. Huigen, E. Jacobsen, A. Pereira, W.J. Stiekema, F. Salamini & C. Gebhardt, 1994. Segregation analysis and RFLP mapping of the R1 and R3 alleles conferring race-specific resistance to Phytophthora infestans in progeny of dihaploid potato parents. Mol Gen Genet 242: 749–754.
El-Kharbotly A., E. Jacobsen, W.J. Stiekema & A. Pereira, 1995. Genetic localisation of transformation competence in diploid potato. Theor Appl Genet 91: 557–562.
Flipse, E., C.J.A.M. Keetels, E. Jacobsen & R.G.F. Visser, 1995. The GBSS-gene has a dosage effect on the starch properties in potato: the influence of amylose on the physico-chemical behaviour of starch. Theor Appl Genet (in press).
Frandsen N.O., 1967. Chimären verschiedener Ploidiestufen aus haploiden Kartoffelklonen. Z Pflanzenzüchtg 57: 123–145.
Freialdenhoven A., B. Scherag, K. Hollricher, D.B. Collinge, H. Thordal-Christensen & P. Schulze-Lefert, 1994. Nar-1 and Nar-2, two loci required for Mla 12-specified race-specific resistance to powdery mildew in barley. Plant Cell 6: 983–994.
Hammond-Kosack K.E., D.A. Jones & J.D.G. Jones, 1994. Identification of two genes required in tomato for full Cf-9-dependent resistance to Cladosporium fulvum. Plant Cell 6: 361–374.
Hermsen J.G.Th. & J. Verdenius, 1973. Selection from Solanum tuberosum group phureja of genotypes combing high frequency haploid induction with homozygosity for embryo spot. Euphytica 22: 244–259.
Hutten R.C.B., E.J.M.M. Scholberg, D.J. Huigen, J.G.T. Hermsen & E. Jacobsen, 1994. Analysis of dihaploid induction and production ability and seed parent x pollinator interaction in potato. Euphytica 72: 61–64.
Jacobsen E., J.H.M. Hovenkamp-Hermelink, H.T. Krijgshield H. Nijdam, L.P. Pijnakker, B. Wilthoult & W.J. Feenstra, 1989. Phenotypic and genotypic characterization of an amylose-free starch mutant of potato. Euphytica 44: 43–48.
Kerber E.R., 1991. Stem-rust resistance in ‘Canthatch’ hexaploid wheat induced by a nonsuppressor mutation on chromosome 7DL. Genome 34: 935–939.
Leonards-Schippers C., W. Gieffers, F. Salamini & C. Gebhardt, 1992. The R1 gene conferring race-specific resistance to Phytophthora infestans in potato is located on potato chromosome V. Mol Gen Genet 233: 278–283.
Malcolmson J.F. & W. Black, 1966. New R genes in Solanum demissum Lindl. and their complementary races of Phytophthora infestans (Mont.) de Bary. Euphytica 15: 199–203.
Mastenbroek C., 1953. Experiments on the inheritance of blight immunity in potatoes derived from Solanum demissum Lindl. Euphytica 2: 197–206.
McKee R.K., 1961. Identification of R-genes in Solanum stoloniferum. Euphytica 11: 42–46.
Ross, H., 1986. Potato breeding-problem and perspectives. J Plant Breeding Suppl 13.
Spielman L.J., J.A. Sweigerd, R.C. Shattock & W.E. Fry, 1990. The genetics of Phytophthora infestans: segregation of allozyme markers in F2 and backcross progeny and the inheritance of virulance against potato resistance genes R2 and R4 in F1 progeny. Experimental Mycology 14: 57–69.
Torp J. & J.H. Jorgensen, 1986. Modification of barley powdery mildew resistance gene Mla 12 by inducing mutation. Can J Genet Cytol 28: 725–731.
Vallejo R.L., W.W. Collins & J.B. Young, 1995. Inheritance of resistance to potato virus Y and potato virus X in hybrid Solanum phureja x S. stenotomum diploid potatoes. J Heredity 86: 89–93.
Van Eck H.J., J.M.E. Jacobs, W.J. Stiekema & E. Jacobsen, 1994. The inheritance of anthocyanin pigmentation in potato (Solanum tuberosum L.) and mapping of tuber skin colour loci using RFLPs. Heredity 73: 410–421.
Wastie R.L., 1991. Breeding for resistance. In: D.S. Ingram & P.H. Williams (Eds.), Advances in Plant Pathology, Vol 7: Phytophthora infestans, the Cause of Late Blight of Potato, pp. 193–223. Academic Press, London, San Diego, New York, Sydney, Tokyo, Toronto.
Wilkinson M.J., S.T. Bennett, S.A. Clulow, J. Allainguillaume, K. Karding & M.D. Bennett, 1995. Evidence for somatic translocation during potato dihaploid induction. Heredity 74: 146–151.
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El-Kharbotly, A., Pereira, A., Stiekema, W.J. et al. Race specific resistance against Phytophthora infestans in potato is controlled by more genetic factors than only R-genes. Euphytica 90, 331–336 (1996). https://doi.org/10.1007/BF00027484
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DOI: https://doi.org/10.1007/BF00027484