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  • Articles  (54)
  • Springer  (52)
  • Canadian Science Publishing  (2)
  • 1980-1984  (54)
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  • Articles  (54)
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  • 11
    Electronic Resource
    Electronic Resource
    Toxicity of certain insecticides to protozoa (1982)
    Springer
    Bulletin of environmental contamination and toxicology 28 (1982), S. 628-631 
    Details
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01605596
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  • 12
    Electronic Resource
    Electronic Resource
    A simple method for obtaining cultures enriched for Type II pneumonocytes from fetal rabbit (1982)
    Springer
    Methods in cell science 7 (1982), S. 63-68 
    Details
    ISSN: 1573-0603
    Keywords: fetal lung ; Type II cells ; culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A differential plating method permitted preparation of cultures significantly enriched for Type II pneumocytes. These cells were maintained in a differentiated state for at least 12 d, identifiable morphologically (by presence of osmiophilic lamellar inclusion bodies) and bio-chemically (by demonstration of synthesis of phosphatidyl choline and production of disaturated lecithin).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01665911
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  • 13
    Electronic Resource
    Electronic Resource
    A simplified method for in situ embedding of monolayer cultures for electron microscopy (1983)
    Springer
    Methods in cell science 8 (1983), S. 41-44 
    Details
    ISSN: 1573-0603
    Keywords: cell culture ; in situ embedding ; electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A simple method for the in situ embedding of cell cultures for electron microscopy has been developed. The procedure is carried out in its entirety within the culture flask, using only standard fixing and embedding reagents. Precoating of the supporting surface of the flask with alternative substrates is not required. This method permits precise orientation of samples with respect to the phase-contrast image of living cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01834634
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  • 14
    Electronic Resource
    Electronic Resource
    Calmodulin-like activity in a mineralising tissue: The rat molar tooth germ (1981)
    Springer
    Calcified tissue international 33 (1981), S. 545-548 
    Details
    ISSN: 1432-0827
    Keywords: calmodulin ; calcium ; mineralisation ; tooth germ
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Calmodulin, a calcium binding protein, has been implicated in the regulation of many calcium-dependent biological processes. Since calcium has an important role in hard tissue genesis, both at intra- and extracellular levels, we anticipate that calcium binding proteins may modulate this process. The present study investigated a mineralising tissue, the rat molar tooth germ, to determine the presence of calmodulin-like activity. A heat-treated cell-free extract of tooth germs provided enhancement of Ca2+-dependent Mg2+-ATPase and 3′:5′-nucleotide phosphodiesterase activity. No enhancement occurred in the absence of calcium or in the presence of trifluoperazine. SDS-polyacrylamide gel electrophoresis of this extract revealed a protein band of approximately 18,000 mol. wt. These findings indicate the presence of calmodulin-like activity in rat molar tooth germs and support the proposal that calcium and calcium binding proteins, in particular calmodulin, have a major regulatory role in the biology of mineralising tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02409487
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  • 15
    Electronic Resource
    Electronic Resource
    Human β-galactosidase and α-neuraminidase deficient mucolipidosis: Genetic complementation analysis of the neuraminidase deficiency (1982)
    Springer
    Human genetics 〈Berlin〉 60 (1982), S. 158-162 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Human β-galactosidase and α-neuraminidase deficient mucolipidosis [ML(gal-neur-)] is an inherited lysosomal enzymopathy which recently was designated as a sialidosis. We analyzed the neuraminidase deficiency of this disorder with genetic complementation analyses using a heterokaryon enrichment procedure. The genetic defects of two apparent variants of this disorder complemented the defects of the neuraminidase deficiency diseases, sialidosis I and mucolipidosis I, resulting in the restoration of neuraminidase activity in heterokaryons. The neuraminidase deficiency, therefore, may not be the primary defect in ML(gal-neur-) and is not an appropriate test for determining carrier status. The clinical and biochemical characteristics of this disorder suggest that a post-translational or processing event for these enzymes may be defective. The defect, however, is different from I-cell disease and pseudo-Hurler polydystrophy, two disorders of post-translational lysosomal enzyme biosynthesis, since complementation studies demonstrated recovery of intracellular β-galactosidase and α-neuraminidase levels in heterokaryons. The lack of human β-galactosidase expression in man-mouse somatic cell hybrids formed from fibroblasts of the infantile onset type disorder suggests that the defect is not corrected by the mouse genome. The ML(gal-neur-) disorder therefore appears to be a distinct subtype of the inherited neuraminidase deficiencies in which the defect may occur in a post-translational or regulatory step which coordinately affects the expression of lysosomal β-galactosidase and α-neuraminidase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00569704
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  • 16
    Electronic Resource
    Electronic Resource
    Cytogerontology since 1881: A reappraisal of August Weismann and a review of modern progress (1982)
    Springer
    Human genetics 〈Berlin〉 60 (1982), S. 101-121 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Cytogerontology, the science of cellular ageing, originated in 1881 with the prediction by August Weismann that the somatic cells of higher animals have limited division potential. Weismann's prediction was derived by considering the role of natural selection in regulating the duration of an organism's life. For various reasons, Weismann's ideas on ageing fell into neglect following his death in 1914, and cytogerontology has only reappeared as a major research area following the demonstration by Hayflick and Moorhead in the early 1960s that diploid human fibroblasts are restricted to a finite number of divisions in vitro. In this review we give a detailed account of Weismann's theory, and we reveal that his ideas were both more extensive in their scope and more pertinent to current research than is generally recognised. We also appraise the progress which has been made over the past hundred years in investigating the causes of ageing, with particular emphasis being given to (i) the evolution of ageing, and (ii) ageing at the cellular level. We critically assess the current state of knowledge in these areas and recommend a series of points as primary targets for future research.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00569695
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  • 17
    Electronic Resource
    Electronic Resource
    Assignment of the glyoxalase II gene (HAGH) to human chromosome 16 (1981)
    Springer
    Human genetics 〈Berlin〉 58 (1981), S. 358-361 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The human and rodent forms of glyoxalase II (hydroxyacylglutathione hydrolase, HAGH) can readily be separated by starch gel electrophoretic procedures. Fifty-one human-rodent somatic cell hybrid clones were examined for their human HAGH and for human enzyme markers whose genes are encoced on each autosome and the X chromosome. Sixteen clones were also examined for their human karyotypes. Human glyoxalase II segregated only with chromosome 16, demonstrating that the gene is located on this chromosome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00282815
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  • 18
    Electronic Resource
    Electronic Resource
    An overview of sedimentation on the amazon continental shelf (1984)
    Springer
    Geo-marine letters 4 (1984), S. 207-210 
    Details
    ISSN: 1432-1157
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract Sedimentological, geochemical, and physical-oceanographic studies of the Brazilian continental shelf near the Amazon River help provide a broad understanding of this major sediment dispersal system. Amazon River sediment accumulates as a subaqueous delta, with the most rapid accumulation (∼10 cm/yr) occurring near the seaward edge of the topset beds and in the foreset beds. Amazon River sediment is dispersed northwestward along the shelf and is transported beyond the Brazilian border. Radiographic studies of sediment cores delineate three sedimentary environments: interbedded mud and sand, faintly laminated mud, and bioturbated mud. The distribution of these environments is a function of proximity to the river mouth and of sediment accumulation rate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02281707
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  • 19
    Electronic Resource
    Electronic Resource
    Discrimination between the red- and far-red-absorbing forms of phytochrome from Avena sativa L. by monoclonal antibodies (1984)
    Springer
    Planta 160 (1984), S. 382-384 
    Details
    ISSN: 1432-2048
    Keywords: Avena (phytochrome) ; Immunological discrimination ; Monoclonal antibody ; Phytochrome (red-, far-red absorbing forms)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A set of rat monoclonal antibodies (ARC MAC 48 to 52 and 54 to 56), raised to phytochrome from dark-grown seedlings of Avena sativa L. was tested for the ability to discriminate between the red-absorbing (Pr) and far-red-absorbing (Pfr) forms of phytochrome by indirect enzyme-linked immunosorbent assay. MAC 50 bound more strongly to Pfr and MAC 49 and 52 showed preferential binding to Pr from extracts of dark-grown Avena seedlings; MAC 50 also bound more strongly to Pfr from brushite-purified phytochrome. The remainder of the monoclonal antibodies and a rabbit polyclonal antiphytochrome preparation did not discriminate between Pr and Pfr. The results provide evidence for conformational changes in defined regions of the phytochrome apoprotein upon photoconversion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00393420
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  • 20
    Electronic Resource
    Electronic Resource
    Regulation of swelling of etiolated-wheat-leaf protoplasts by phytochrome and gibberellic acid (1983)
    Springer
    Planta 158 (1983), S. 416-421 
    Details
    ISSN: 1432-2048
    Keywords: Gibberellin and protoplast swelling ; Phytochrome ; Protoplast (swelling) ; Triticum (protoplast swelling)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of light on the size of intact protoplasts isolated from the primary leaves of etiolated Triticum aestivum was studied. A 2-min red-light irradiation in the presence of 1 mM KCl was sufficient to cause a swelling of protoplasts compared with those maintained in darkness. The effect was photoreversible by far-red light over two light cycles, indicating the involvement of phytochrome. At 4°C, escape from reversibility occurred between 2 and 5 min after the exposure to red light. In exposure-response experiments, 20 s red light at 27 μmol m-2s-1 was sufficient to saturate the response. Exogenous gibberellic acid added in darkness in the presence of KCl also induced protoplast swelling. Gibberellins may act as an intermediate in the phytochrome-induced swelling of protoplasts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00397734
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