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  • SOLAR PHYSICS  (614)
  • FLUID MECHANICS AND HEAT TRANSFER  (464)
  • Cell & Developmental Biology
  • 1980-1984  (834)
  • 1975-1979  (620)
Collection
Keywords
Publisher
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Perturbation of the switch-on of transcriptase activity in intermediate subviral particles from reovirus (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 112 (1982), S. 10-18 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Intermediate subviral particles (ISVP) derived from reovirus represent a simple model system for the switch-on of transcriptase function. In such particles the endogenous transcriptase is present in a switched-off form, one step removed from the switched-on state. Switch-on of transcriptase function is an active process in this system and can be triggered by K+ ions. A variety of agents which affect gene expression in cells were tested for an effect on switch-on in ISVP. Marked effects on switch-on in ISVP were observed with a diverse group of test agents, including DMSO and other solvents, BUdR, TdR, caffeine, theophylline, and temperature. The correlation in response between ISVP and cells suggests that the ISVP system may be useful as a model for studying the biochemical mechanisms underlying the perturbative effects of such agents on gene expression in cells.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041120104
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Morphological and ultrastructural characterization of mammalian spermatozoa processed for flow cytometric DNA analyses (1984)
    New York, NY : Wiley-Blackwell
    Gamete Research 10 (1984), S. 339-351 
    Details
    ISSN: 0148-7280
    Keywords: spermatozoa ; flow cytometry ; DNA staining ; nuclear morphology ; ultrastructure ; mammals ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The morphological and ultrastructural changes that occur during preparation of porcine, bovine, and murine spermatozoa for flow cytometric quantification of the relative DNA content of the X- and Y-chromosome-bearing sperm populations were examined. Ejaculated spermatozoa from the boar and bull were washed using a series of dimethyl sulfoxide (DMSO) solutions prior to fixation, whereas the epididymal mouse spermatozoa were washed only in phosphate-buffered saline (PBS). Spermatozoa from all three species were then fixed in ethanol and processed for fluorochrome staining by a treatment regimen consisting of sulfhydryl reduction and proteolysis. The processed sperm nuclei were stained for DNA with the fluorochrome, 4′-6-diamidino-2-phenylindole (DAPI) before quantification by flow cytometry. Scanning and transmission electron micrographs of sperm heads taken at various steps of the preparation and staining procedures show 1) that the rigorous washing procedure disrupted the plasma and outer acrosomal membranes, 2) that ethanol fixation resulted in removal of the outer membranes and disintegration of the nuclear envelope, and 3) that thiol and proteolysis treatment removed the remaining cellular organelles including the tail and rapidly induced partial decondensation of the tightly packed chromatin. Sequential micrographs showed that the nuclear matrix of all three species increased in thickness about twofold during the preparation and staining. Consequently, the harsh procedures currently used for quantitative staining of DNA for high-resolution flow cytometric analyses destroy most cellular organelles and thereby prevent simultaneous characterization of DNA content and other sperm cell constituents.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120100402
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Inhibition of the metabolism of ram spermatozoa by derivatives of α-chlorohydrin (1981)
    New York, NY : Wiley-Blackwell
    Gamete Research 4 (1981), S. 203-217 
    Details
    ISSN: 0148-7280
    Keywords: α-chlorohydrin ; antifertility agent ; ram ; sperm metabolism ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The effects of the male antifertility agent, α-chlorohydrin, six of its derivatives, and glycidol were studied on the metabolism of washed ram spermatozoa in vitro with fructose as substrate. The α-chlorohydrin derivatives were the amino, the phosphorylated, and four glycol-bridge (ketal) compounds. All compounds except glycidol, in a concentration between 0.1 and 100 mM, reduced the aerobic glycolsis and/or oxidation of fructose. However, there was not a high correlation between the ability of these compounds to inhibit the metabolism of ram spermatozoa in vitro and their antifertility activity when administered to male rats. Other factors are clearly involved in their antifertility activity, eg, the concentration of the compounds in the epididymis and their conversion of either more or less spermicidal compounds in the body.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120040305
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  • 4
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    Hypersonic heat-transfer and transition correlations for a roughened shuttle orbiter (1976)
    In:  CASI
    Details
    Publication Date: 2016-06-07
    Description: The effect of roughness on the heat transfer distributions and the transition criteria for the windward pitch plane of the shuttle orbiter at an angle of attack of 30 deg was studied using data obtained in hypersonic wind tunnels. The heat transfer distributions and the transition locations for the roughened models were compared with the corresponding data for smooth models. The data were correlated using theoretical solutions for a nonsimilar, laminar boundary layer subject to two different flow field models for the orbiter.
    Keywords: FLUID MECHANICS AND HEAT TRANSFER
    Type: an. In Eng. Sci., Vol. 4; p 1615-1627
    Format: application/pdf
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    NASA TECHNICAL REPORTS
  • 5
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    Observations of a probable change in the solar radius between 1715 and 1979 (1980)
    In:  Other Sources
    Details
    Publication Date: 2011-08-18
    Description: A decrease in the solar radius is determined using the technique of Dunham and Dunham (1973), in which timed observations are made just inside the path edges. When the method is applied to the solar eclipses of 1715, 1976, and 1979, the solar radius for 1715 is 0.34 + or - 0.2 arc second larger than the recent values, with no significant change between 1976 and 1979. The duration of totality is examined as a function of distance from the edges of the path. Corrections to the radius of the sun derived from observations of the 1976 and 1979 eclipses by the International Occultation Timing Association are also presented.
    Keywords: SOLAR PHYSICS
    Type: Science; 210; Dec. 12
    Format: text
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  • 6
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    Diode laser heterodyne observations of silicon monoxide in sunspots - A test of three sunspot models (1983)
    In:  Other Sources
    Details
    Publication Date: 2019-06-28
    Description: Absorption features from the 8 micron SiO fundamental (upsilon = 1-0) and hot bands (upsilon = 2-1) have been observed in sunspots at sub-Doppler resolution using a ground-based tunable diode laser heterodyne spectrometer. The observed line widths suggest an upper limit of 0.5 km/s for the microturbulent velocity in sunspot umbrae. Since the silicon monoxide abundance is very sensitive to sunspot temperature, the measured equivalent widths permit an unambiguous determination of the temperature-pressure relation in the upper layers of the umbral atmosphere. In the region of SiO line formation (log P sub g = 3.0-4.5), the results support the sunspot model suggested by Stellmacher and Wiehr (1970).
    Keywords: SOLAR PHYSICS
    Type: Astrophysical Journal, Part 1 (ISSN 0004-637X); 269; June 1
    Format: text
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  • 7
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    Mean flowfields in axisymmetric combustor geometries with swirl (1983)
    In:  Other Sources
    Details
    Publication Date: 2019-06-28
    Description: (Previously cited in issue 06, p. 860, Accession no. A82-17824)
    Keywords: FLUID MECHANICS AND HEAT TRANSFER
    Format: text
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  • 8
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    Coronal changes associated with a disappearing filament (1975)
    In:  Other Sources
    Details
    Publication Date: 2019-06-27
    Description: Skylab/ATM observations of a disappearing filament near the center of the solar disk are described using XUV and H-alpha spectroheliograms, X-ray filterheliograms, and photospheric magnetograms. The temperature of the coronal plasma as the filament disappeared is estimated to have been in excess of 6 million K, and it is noted that the time history of the soft X-ray and microwave fluxes displayed the gradual-rise-and-fall (GRF) signature, suggesting that the present event may have properties that are characteristic of a wide class of long-duration X-ray and radio events. A comparison with other spatially resolved long-duration X-ray events indicates that all such long-lived bursts involve transients in the outer corona and that some two-thirds of them involve either the eruption or the major activation of a prominence. It is also found that long-lived events are characterized by the appearance of new emission loops in the lower corona during the declining phase of the X-ray emission and that these loops sometimes disappear after the X-ray events and sometimes remain indefinitely.
    Keywords: SOLAR PHYSICS
    Type: Solar Physics; 45; Dec. 197
    Format: text
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  • 9
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    Mean flowfields in axisymmetric combustor geometries with swirl (1982)
    In:  Other Sources
    Details
    Publication Date: 2019-07-13
    Description: Six flowfield configurations are investigated with sidewall angles of 90 and 45 deg, and swirl vane angles of 0, 45, and 70 deg. It is found that central recirculation zones occur for the swirling flow cases investigated, which extend from the inlet to x/D = 1.7, where x is the axial polar coordinate, and D is the test section diameter. Five-hole pitot probe pressure measurements are used to determine time-mean velocities, and corresponding flow situations are predicted and compared to results of experimental data. Excellent agreement is found for the nonswirling flow, although poor agreement is found for swirling flow cases, especially near the inlet. The discrepancy is attributed to the lack of realism in the turbulence model, and/or to inaccurate specification of time-mean velocity and turbulence energy distributions at the inlet.
    Keywords: FLUID MECHANICS AND HEAT TRANSFER
    Type: AIAA PAPER 82-0177 , Aerospace Sciences Meeting; Jan 11, 1982 - Jan 14, 1982; Orlando, FL
    Format: text
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  • 10
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    Heat transfer characteristics for inline and staggered arrays of circular jets with crossflow of spent air (1978)
    In:  Other Sources
    Details
    Publication Date: 2019-07-13
    Description: Heat transfer characteristics were measured for two dimensional arrays of jets impinging on a surface parallel to the jet orifice plate. The impinging flow was constrained to exit in a single direction along the channel formed by the jet plate and the heat transfer surface. Both mean Nusselt numbers and streamwise Nusselt number profiles are presented as a function of Reynolds number and geometric parameters. These are the streamwise and transverse hole spacings ranging from 5 to 10 and 4 to 8 jet orifice diameters, respectively; the channel height ranging from 1 to 6 diameters; and the hole pattern which includes both inline and staggered arrays. The results show that significant periodic variations occur in the streamwise Nusselt number profiles, persisting downstream for at least ten rows of jet holes. Channel height can have a significant effect on the chordwise profiles, smoothed across the periodic variations. For the smaller channel heights, Nusselt numbers first decrease and then increase downstream. Where significant differences exist, inline hole patterns provide better heat transfer than staggered ones. These and other effects of the geometric parameters are presented and discussed.
    Keywords: FLUID MECHANICS AND HEAT TRANSFER
    Type: Gas turbine heat transfer 1978; Dec 10, 1978 - Dec 15, 1978; San Francisco, CA
    Format: text
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