ALBERT

Notes: Abstract Fowl plague virus, strain Dutch, was metabolically labeled withd-[2-3H]mannose, or withd-[6-3H]glucosamine, and the small subunit (HA2; 0.8 mg in total) of the viral hemagglutinin was isolated by preparative sodium dodecylsulfate-polyacrylamide gel electrophoresis. After proteolytic digestion, the radioactive oligosaccharides were sequentially liberated from the glycopeptides by treatment with different endo-β-N-acetylglucosaminidases and with peptide:N-glycosidase or, finally, by hydrazinolysis. In this manner, four groups of glycans could be obtained by consecutive gel filtrations and were subfractionated by HPLC. The structures of the individual oligosaccharides were analyzed by micromethylation, by acetolysis or by digestion with exoglycosidases. The major species amongst the high mannose glycans at Ans-406 of the viral glycopolypeptide were found to be Manα1-2Manα1-3(Manα1-2Manα1-6)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNacβ1-4GlcNAc and Manα1-3(Manα1-2Manα1-6)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc, while the complex glycans at Asn-478 are predominantly GlcNAcβ1-2Manα1-3(GlcNAcβ1-2Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAc (lacking, in part, one of the outerN-acetylglucosamine residues) and GlcNAcβ1-2Manα1-3(Galβ1-4GlcNAcβ1-2Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAc.

Notes: Abstract U, B, V-observations, supplemented by spectrophotometric measurements of the symbiotic variable CH Cyg in the years 1982 to 1984 show that the star has reached its ‘blue maximum phase’ of its recent outburst in the first half of 1984. From end of July, 1984 on the object underwent a considerable drop in brightness and an increase of its colour indices, and which indicates that it was on the way to its cool phase. Also the spectroscopic observations show that the ‘blue’ continuum still observed at the beginning of July, 1984 has disappeared in October–November, 1984 and the strong absorption features, typical for a M-type giant were re-appearing. A remarkable intensity increase of the emission features, especially those of the Balmer lines, took place within this time interval. The rapid flickering variations of CH Cyg had also reached the culmination amplitude in July, 1984. There seem to be three periodicities present in these flickering variations with cycle lengths of 7.7 min, 21 min, and 60 min. Though these observational findings would be consistent with the M-type blue subdwarf component binary model with mass flow from the M6-III giant to the companion, the genuine binary nature of CH Cyg could not be established beyond doubt.

Notes: Abstract The discovery of magnetic field strengths in the range of 500 million Gauss in the objects Grw+70°8247 and PG1031+224 has given enormous impetus to investigations of magnetic white dwarf stars. We have determined intensities of Balmer transitions for B〉108 Gauss as a function of field and find strong oscillations of the transitions strengths, which are of importance to the quantitative analysis of the observed spectra and the interpretation of polarimetric measurements in these objects.

Notes: Abstract Beside some historical notes about the large Fraunhofer's heliometer used by Bessel and Argelander, some modern applications of the heliometer principle for the geometric and photometric autocalibration of detectors and the determination of absolute radial velocities with slitless field spectrographs are presented.

Notes: Abstract The vibrational-rotational spectra of the fundamentals of nitrosyl chloride have been recorded with a Fourier transform interferometer at an apodized resolution of 0.004 cm−1. The data have been analyzed to determine excited state molecular constants forv 1 v 2, andv 3.

Notes: Zusammenfassung Beryllium gibt mit Chromazurol S auf Filtrierpapier einen charakteristischen hellblauen Fleck. Der störende Einfluß anderer Kationen kann durch Zugabe von Oxalat und ÄDTA behoben werden. Es lassen sich noch 4,5μg Beryllium in 0,05 ml Lösung nachweisen. Bei zehnfachem Überschuß einiger Kationen wird die Empfindlichkeit der Reaktion verringert.

Notes: Abstract The application of a special version of the nonlocal light-cone expansion to hadron processes with large momentum transfer is reviewed. The light-cone operators introduced in this approach may be considered as the relevant hadron operators: Their matrix elements between the vacuum and a one-hadron state coincide with the quark distribution amplitude, and their anomalous dimensions are unique extensions of the well known Brodsky-Lepage evolution kernels having also a simple relation to the Altarelli-Parisi kernels.

Notes: Summary The extensive genetical analysis of the T-locus in mice, carried out by Dunn and his co-workers, has revealed that the breeding pattern of t-alleles is very peculiar. The complexity of the T-locus indicates that some structural rearrangements are involved here. The purpose of the present work was to gain some informations on the structure of chromosomes in which t-alleles are present. 2. The course of spermatogenesis in the lines of mice of the following genotypes was investigated: +/+; T/+; T/t n — namely T/t 0, T/t12, T/tw1, T/tw6, T/tw18; tn/tx — namely t 0/t12, t0/tw18, t12/tw18, tw1/t12, tw1/t0, tw1/tw6, tw1/tw18, and in heterozygotes for translocations T138 and T190, both involving the fragment of linkage group IX in which some t-alleles are present and used as markers. 3. The alleles T and t w1 are not connected with structural rearrangements, as far as detectable by the methods used. The alleles t 0, tw6 and t w18 are interstitial deficiencies involving different chromosomal regions. The most complete data were obtained for t 0: in T/t 0 heterozygotes a loop corresponding to a big interstitial deficiency was present, in all t 0/tn compounds the aberrant structure of one of the bivalents indicated that an interstitial deficiency is here involved. By analysis of translocation T190 the deficiency loop is localized in the chromosome representing linkage group IX. The identification of t w6 as a small interstitial deficiency is based on the analysis of T/t w6 heterozygotes and its localization in linkage group IX on the presence of this deficiency in translocation T138. The description of tw18 as a small interstitial deficiency stems from the analysis of T/tw18 and t 0/tw18, the localization in linkage group IX is shown by its presence in t 0/tw18 in the same bivalent as t 0. The evidence obtained for t 12 indicates that it is either a big terminal deficiency or a small interstitial one inducing constant asynapsis in adjacent terminal segments of the bivalent. 4. The reasons why loops encountered in genotypes involving t 0, tw6 and t w18 are considered as deficiencies and not as duplications are based on available embryological and genetical data. The possible connections between the mode of action of the investigated t-alleles as embryonic lethals and the cytological results on their structure and localization are discussed. 5. Most of the lethal t-alleles suppress crossing-over in the region T-tf. Factors reducing the frequency of recombination in other organisms are discussed in comparison. It is concluded that the pattern of aberation in meiotic behavior of the bivalent carrying t-alleles is for almost each of the studied alleles rather specific and does not permit for suggesting any general mechanism which could account for the action of investigated lethal t-alleles as crossing-over suppressors. 6. A diagram of the presumable localisation of genetical factors in linkage group IX is presented. It is concluded that the so-called T-locus occupies the main part of the long arm of the chromosome corresponding to linkage group IX and that the investigated t-alleles are not unilocal neither with the gene T nor each with other, but scattered along the chromosome. 7. The possible modes of the origin of new t-alleles are discussed, and the hypothesis is advanced that this event might be connected with mistakes of replication of the strand on which a new t-allele arises.