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Carbohydrates of influenza virus. Structure of the oligosaccharides linked to asparagines 406 and 478 in the hemagglutinin of fowl plague virus, strain dutch

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Abstract

Fowl plague virus, strain Dutch, was metabolically labeled withd-[2-3H]mannose, or withd-[6-3H]glucosamine, and the small subunit (HA2; 0.8 mg in total) of the viral hemagglutinin was isolated by preparative sodium dodecylsulfate-polyacrylamide gel electrophoresis. After proteolytic digestion, the radioactive oligosaccharides were sequentially liberated from the glycopeptides by treatment with different endo-β-N-acetylglucosaminidases and with peptide:N-glycosidase or, finally, by hydrazinolysis. In this manner, four groups of glycans could be obtained by consecutive gel filtrations and were subfractionated by HPLC. The structures of the individual oligosaccharides were analyzed by micromethylation, by acetolysis or by digestion with exoglycosidases. The major species amongst the high mannose glycans at Ans-406 of the viral glycopolypeptide were found to be Manα1-2Manα1-3(Manα1-2Manα1-6)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNacβ1-4GlcNAc and Manα1-3(Manα1-2Manα1-6)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc, while the complex glycans at Asn-478 are predominantly GlcNAcβ1-2Manα1-3(GlcNAcβ1-2Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAc (lacking, in part, one of the outerN-acetylglucosamine residues) and GlcNAcβ1-2Manα1-3(Galβ1-4GlcNAcβ1-2Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAc.

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Abbreviations

BSA:

bovine serum albumin

endo D (F,H):

endo-β-N-acetyl-d-glucosaminidase D (F,H)

HA:

hemagglutinin (HA1, large subunit of HA

HA2 :

small subunit

FPV:

fowl plague virus

PNGase F:

peptide:N-glycosidase F

SDS:

sodium dodecylsulfate

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Authors and Affiliations

  1. Biochemisches Institut am Klinikum der Universität, Friedrichstrasse 24, D-6300, Giessen, Federal Republic of Germany

    Rudolf Geyer, Silvia Diabaté, Hildegard Geyer & Stephan Stirm

  2. Institut für Medizinische Virologie am Klinikum der Universität, Frankfurter Strasse 107, D-6300, Giessen, Federal Republic of Germany

    Hans-Dieter Klenk & Heiner Niemann

Authors
  1. Rudolf Geyer
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  2. Silvia Diabaté
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  3. Hildegard Geyer
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  4. Hans-Dieter Klenk
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  5. Heiner Niemann
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  6. Stephan Stirm
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Geyer, R., Diabaté, S., Geyer, H. et al. Carbohydrates of influenza virus. Structure of the oligosaccharides linked to asparagines 406 and 478 in the hemagglutinin of fowl plague virus, strain dutch. Glycoconjugate J 4, 17–32 (1987). https://doi.org/10.1007/BF01048441

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  • DOI: https://doi.org/10.1007/BF01048441

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