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  • Isoprenoid induction  (1)
  • Isotope labeling  (1)
  • Karplus parametrization  (1)
  • Multiplet simulation  (1)
  • Springer  (4)
  • 1995-1999  (4)
  • 1985-1989
Collection
Keywords
Publisher
  • Springer  (4)
Years
  • 1995-1999  (4)
  • 1985-1989
Year
  • 1
    Electronic Resource
    Electronic Resource
    Application of H(N)CA,CO-E.COSY experiments for calibrating the φ angular dependences of vicinal couplings J(C′i−1,Hi α), J(C′i−1,Ci β) and J(C′i−1,C′i) in proteins (1997)
    Springer
    Journal of biomolecular NMR 10 (1997), S. 107-118 
    Details
    ISSN: 1573-5001
    Keywords: Vicinal coupling constants ; φ Torsion angle ; Karplus parametrization ; Desulfovibrio vulgaris flavodoxin ; Isotopic labelling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A triple-resonance NMR technique suitable for the determination ofcarbonyl-related couplings in polypeptide systems is introduced. Theapplication of three novel pulse sequences to uniformly13C/15N-enriched proteins yields E.COSY-likemultiplet patterns exhibiting either one of the3J(C′i−1,Hi α), 3J(C′i−1,Ci β) and3J(C′i−1,C′i)coupling constants in the indirectly detected 13C′dimension, depending on the passive spin selected. The experiments aredemonstrated with oxidized flavodoxin from Desulfovibrio vulgaris. On thebasis of the J-values measured and the backbone φ-angles derived from ahigh-resolution X-ray structure of the protein, the three associated Karplusequations were reparametrized. The root-mean-square differences between theexperimental coupling constants and those predicted by the optimized Karpluscurves are 0.41, 0.33 and 0.32 Hz for3J(C′i−1,Hi α),3J(C′i−1,Ci β) and3J(C′i−1,C′i),respectively. The results are compared with the Karplus parameters previouslypublished for the same couplings.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018355327792
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  • 2
    Electronic Resource
    Electronic Resource
    Determination of heteronuclear three-bond J-coupling constants in peptides by a simple heteronuclear relayed E.COSY experiment (1995)
    Springer
    Journal of biomolecular NMR 6 (1995), S. 95-105 
    Details
    ISSN: 1573-5001
    Keywords: 2D heteronuclear NMR ; Isotope labeling ; Coupling constants ; Antamanide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary A simple heteronuclear relayed E.COSY pulse sequence with a minimum number of pulses is proposed for the quantitative determination of heteronuclear three-bond J-coupling constants in uniformly 13C-enriched polypeptide samples. Numerous heteronuclear three-bond coupling constants, including $${}^3{\text{J}}_{{\text{H}}^{\text{N}} {\text{C}}'} $$ , $${}^3{\text{J}}_{{\text{H}}^{\text{N}} {\text{C}}^\beta } $$ , $${}^3{\text{J}}_{{\text{H}}^\beta {\text{C}}'} $$ , and $${}^3{\text{J}}_{{\text{H}}^\alpha {\text{C}}^\gamma } $$ , can be determined for each residue from a single heteronuclear relayed E.COSY spectrum. Couplings relevant for stereospecific assignments as well as for the determination of dihedral angles in the amino acid backbone and in side chains are obtained. The method is demonstrated on the uniformly 13C-enriched decapeptide antamanide (-Val1-Pro2-Pro3-Ala4-Phe5-Phe6-Pro7-Pro8-Phe9-Phe10-).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00417495
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  • 3
    Electronic Resource
    Electronic Resource
    Heteronuclear relayed E.COSY applied to the determination of accurate 3J(HN,C′) and 3J(Hβ,C′) coupling constants in Desulfovibrio vulgaris flavodoxin (1996)
    Springer
    Journal of biomolecular NMR 7 (1996), S. 142-152 
    Details
    ISSN: 1573-5001
    Keywords: Multiplet simulation ; Least-squares optimization ; Spectrum fitting ; Long-range couplings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary A simple constant-time 3D heteronuclear NMR pulse sequence has been developed to quantitatively determine the heteronuclear three-bond couplings 3J(HN,C′) and 3J(Hβ,C′) in uniformly 13C-enriched proteins. The protocols for measuring accurate coupling constants are based on 1H,13C-heteronuclear relayed E.COSY [Schmidt, J.M., Ernst, R.R., Aimoto, S. and Kainosho, M. (1995) J. Biomol. NMR, 6, 95–105] in combination with numerical least-squares spectrum evaluation. Accurate coupling constants are extracted from 2D spectrum projections using 2D multiplet simulation. Confidence intervals for the obtained three-bond coupling constants are calculated from F-statistics. The three-bond couplings are relevant to the determination of ϕ and X 1 dihedral-angle conformations in the amino acid backbone and side chain. The methods are demonstrated on the recombinant 13C, 15N-doubly enriched 147-amino acid protein Desulfovibrio vulgaris flavodoxin with bound flavin mononucleotide in its oxidized form. In total, 109 3J(HN,C′) and 100 3J(Hβ,C′) coupling constants are obtained from a single spectrum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00203824
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  • 4
    Electronic Resource
    Electronic Resource
    The arbuscular mycorrhizal fungus, Glomus intraradices, induces the accumulation of cyclohexenone derivatives in tobacco roots (1999)
    Springer
    Planta 207 (1999), S. 620-623 
    Details
    ISSN: 1432-2048
    Keywords: Key words: Arbuscular mycorrhiza ; Blumenol derivative ; Cyclohexenone derivative ; Glomus ; Nicotiana ; Isoprenoid induction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Tobacco (Nicotiana tabacum L.) plants were grown with and without the arbuscular mycorrhizal fungus, Glomus intraradices Schenk & Smith. High-performance liquid chromatographic analyses of methanolic extracts from mycorrhizal and non-mycorrhizal tobacco roots revealed marked fungus-induced changes in the patterns of UV-detectable products. The UV spectra of these products, obtained from an HPLC photodiode array detector, indicated the presence of several blumenol derivatives. The most predominant compound among these derivatives was spectroscopically identified as 13-hydroxyblumenol C 9-O-gentiobioside (“nicoblumin”), i.e. the 9-O-(6′-O-β-glucopyranosyl)-β-glucopyranoside of 13-hydroxy-6-(3-hydroxybutyl)-1,1,5-trimethyl-4-cyclohexen-3-one, a new natural product. This is the first report on the identification of blumenol derivatives in mycorrhizal roots of a non-gramineous plant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004250050526
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