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  • 1
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    Functional annotation of a full-length Arabidopsis cDNA collection (2002)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2002-03-23
    Description: Full-length complementary DNAs (cDNAs) are essential for the correct annotation of genomic sequences and for the functional analysis of genes and their products. We isolated 155,144 RIKEN Arabidopsis full-length (RAFL) cDNA clones. The 3'-end expressed sequence tags (ESTs) of 155,144 RAFL cDNAs were clustered into 14,668 nonredundant cDNA groups, about 60% of predicted genes. We also obtained 5' ESTs from 14,034 nonredundant cDNA groups and constructed a promoter database. The sequence database of the RAFL cDNAs is useful for promoter analysis and correct annotation of predicted transcription units and gene products. Furthermore, the full-length cDNAs are useful resources for analyses of the expression profiles, functions, and structures of plant proteins.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Seki, Motoaki -- Narusaka, Mari -- Kamiya, Asako -- Ishida, Junko -- Satou, Masakazu -- Sakurai, Tetsuya -- Nakajima, Maiko -- Enju, Akiko -- Akiyama, Kenji -- Oono, Youko -- Muramatsu, Masami -- Hayashizaki, Yoshihide -- Kawai, Jun -- Carninci, Piero -- Itoh, Masayoshi -- Ishii, Yoshiyuki -- Arakawa, Takahiro -- Shibata, Kazuhiro -- Shinagawa, Akira -- Shinozaki, Kazuo -- New York, N.Y. -- Science. 2002 Apr 5;296(5565):141-5. Epub 2002 Mar 21.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center (GSC), 3-1-1 Koyadai, Tsukuba 305-0074, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11910074" target="_blank"〉PubMed〈/a〉
    Keywords: Arabidopsis/*genetics/metabolism ; Arabidopsis Proteins/genetics/metabolism ; Chromosome Mapping ; Cloning, Molecular ; Computational Biology ; *DNA, Complementary/isolation & purification ; DNA, Plant/genetics ; Databases, Nucleic Acid ; *Expressed Sequence Tags ; Gene Expression ; Gene Library ; *Genes, Plant ; Genome, Plant ; Promoter Regions, Genetic ; RNA, Messenger/genetics ; Sequence Analysis, DNA
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 2
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    Redox regulation of germline and vulval development in Caenorhabditis elegans (2003)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2003-12-06
    Description: In vitro studies have indicated that reactive oxygen species (ROS) and the oxidation of signaling molecules are important mediators of signal transduction. We have identified two pathways by which the altered redox chemistry of the clk-1 mutants of Caenorhabditis elegans acts in vivo on germline development. One pathway depends on the oxidation of an analog of vertebrate low density lipoprotein (LDL) and acts on the germline through the Ack-related tyrosine kinase (ARK-1) kinase and inositol trisphosphate (IP3) signaling. The other pathway is the oncogenic ras signaling pathway, whose action on germline as well as vulval development appears to be modulated by cytoplasmic ROS.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Shibata, Yukimasa -- Branicky, Robyn -- Landaverde, Irene Oviedo -- Hekimi, Siegfried -- New York, N.Y. -- Science. 2003 Dec 5;302(5651):1779-82.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biology, McGill University, 1205 Avenue Docteur Penfield, Montreal, Quebec, Canada, H3A 1B1.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/14657502" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Sequence ; Animals ; Apolipoproteins B/genetics/metabolism ; Base Sequence ; Caenorhabditis elegans/genetics/*growth & development/*metabolism ; Caenorhabditis elegans Proteins/chemistry/genetics/metabolism ; Cholesterol/metabolism ; Cloning, Molecular ; Disorders of Sex Development ; Female ; Inositol Phosphates/metabolism ; Lipoproteins, LDL/*metabolism ; Molecular Sequence Data ; Mutation ; Oxidation-Reduction ; Phenotype ; Protein-Tyrosine Kinases/metabolism ; RNA Interference ; Reactive Oxygen Species/*metabolism ; Transcription Factors/genetics/metabolism ; Vulva/growth & development ; ras Proteins/genetics/metabolism
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
    Location Call Number Expected Availability
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