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  • thermal diffusivity  (6)
  • Key words Oryza sativa L.  (3)
  • phylogeny  (3)
  • Springer  (12)
  • Blackwell Publishing Ltd
  • 2000-2004  (12)
Collection
Keywords
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  • Springer  (12)
  • Blackwell Publishing Ltd
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Year
  • 1
    ISSN: 1432-2242
    Keywords: Key words Oryza sativa L. ; Ratoon ; Molecular marker ; QTL
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Although F2s are the most informative populations for genetic analysis, it has been difficult to use F2 populations directly for QTL analysis because it is usually difficult to assess the reliability of the data, due to an inability to estimate the experimental errors. In this study, we performed a QTL analysis for yield and yield-component traits of an F2 population based on data from replicated field trials over 2 years using vegetative shoots of ratooned plants, making use of the ratooning habit of rice. The objective of this study was to explore the possibility of conducting QTL analyses directly based on an F2 population by means of ratooning plants. The experimental population was from a cross between ’Zhenshan 97’ and ’Minghui 63’, the parents of ’Shanyou 63’, an elite rice hybrid widely grown in China. A genetic linkage map containing 151 molecular markers was constructed for QTL mapping. A total of 20 distinct QTLs were detected; eight of these were detected in both years and remaining 12 in only 1 year. Compared with the results of our previous analysis of the F2:3 families from the same cross, it was shown that most of the QTLs detected in the ratooned F2 population were also detected in the F2:3 population. However, the estimates of both additive and dominant types of genetic effects for many of the QTLs based on F2 ratoons were substantially larger than those based on F2:3 families. The results indicate that vegetatively ratooned F2 populations may have considerable utility in the mapping of QTLs, especially if dominant types of gene actions are of concern, although there were certain technical limitations in making use of such populations in the experiments.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1572-9567
    Keywords: flash method ; thermal contact resistance ; thermal control coatings ; thermal diffusivity ; two-layer composite sample
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The thermal diffusivity of brittle coatings cannot be measured by the flash method directly because of the difficulty of preparing free-standing samples. Adopting the flash method using a two-layer composite sample, it is possible to measure thermal diffusivity if the radiant pulse is well defined and good thermal contact on the interface of the composite sample can be ensured. Using an equilateral trapezoidal pulse of an Nd-glass laser measuring the dimensionless temperature history of the rear face of the sample, we determined the thermal diffusivity of thermal control coatings in the temperature range of 80 to 200°C. The results for different thicknesses of substrate showed that the thermal contact resistance of the interface can be neglected.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    International journal of thermophysics 21 (2000), S. 479-485 
    ISSN: 1572-9567
    Keywords: diamond ; film ; flash method ; thermal diffusivity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract This paper discusses the short-pulse-flash method developed for thermal diffusivity measurements on thin films. Two kinds of CVD diamond film have been prepared, and their thermal diffusivity in the perpendicular direction has been measured with this method. The measurement errors caused by the surface coating are discussed.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell reports 19 (2000), S. 321-326 
    ISSN: 1432-203X
    Keywords: Key words Oryza sativa L. ; Egg cell ; Zygote ; Central cell ; Isolation ; Microculture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  All component cells of the embryo-sac before and after fertilization in rice were isolated by manual microdissection under conditions either free of enzymes or combined with a short pulse of enzymatic treatment.In general, the frequency of isolated unfertilized or fertilized egg cells or central cells reached 15–40%. Various component cells of the embryo-sac after isolation were distinguished by their own morphological characteristics. The isolated cells were cultured in a microchamber and fed with dividing rice suspension cells. Both unfertilized and fertilized egg cells and central cells were induced to divide. Among them only the fertilized egg cells (the zygotes) developed into proembryo-like multicellular structures. The frequency of the first zygotic division and the frequency of multicellular structures were higher using the non-enzymatic method than using the enzymatic one.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 100 (2000), S. 1295-1303 
    ISSN: 1432-2242
    Keywords: Key words Oryza sativa L. ; AFLP markers ; RFLP markers ; Aluminum tolerance ; QTLs ; Epistasis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  To investigate the genetic background for aluminum (Al) tolerance in rice, a recombinant inbred (RI) population, derived from a cross between an Al-sensitive lowland indica rice variety IR1552 and an Al-tolerant upland japonica rice variety Azucena, was used in culture solution. A molecular linkage map, together with 104 amplified fragment length polymorphism (AFLP) markers and 103 restriction fragment length polymorphism (RFLP) markers, was constructed to map quantitative trait loci (QTLs) and epistatic loci for Al tolerance based on the segregation for relative root length (RRL) in the population. RRL was measured after stress for 2 and 4 weeks at a concentration of 1mM of Al3+ and a control with a pH 4.0, respectively. Two QTLs were detected at both the 2nd and the 4th weeks on chromosomes 1 and 12 from unconditional mapping, while the QTL on chromosome 1 was only detected at the 2nd stress week from conditional mapping. The effect of the QTL on chromosome 12 was increased with an increase of the stress period from 2 to 4 weeks. The QTL on chromosome 1 was expressed only at the earlier stress, but its contribution to tolerance was prolonged during growth. At least one different QTL was detected at the different stress periods. Mean comparisons between marker genotypic classes indicated that the positive alleles at the QTLs were from the Al-tolerant upland rice Azucena. An important heterozygous non-allelic interaction on Al tolerance was found. The results indicated that tolerance in the younger seedlings was predominantly controlled by an additive effect, while an epistatic effect was more important to the tolerance in older seedlings; additionally the detected QTLs may be multiple allelic loci for Al tolerance and phosphorus-uptake efficiency, or for Al and Fe2+ tolerance.
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  • 6
    ISSN: 1615-6110
    Keywords: Ranunculaceae ; Aconitum ; ITS ; polymorphism ; pseudogenes ; phylogeny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA (nrDNA) were used to examine the phylogeny of East Asian aconites. Individual aconites were discovered to contain as many as eight different ITS sequences after cloning and PCR-SSCP (single-stranded conformational polymorphisms) analysis. We identified eight putative ITS pseudogenes from four taxa with low predicted secondary structure stability and high substitution rates. Maximum likelihood (ML) and neighbor-joining (NJ) methods were used for phylogenetic reconstruction. The ITS trees agree with the previous chloroplast DNA (cpDNA) tree for the vast majority of the taxa. We found two East Asian clades in the ITS trees: 1) a clade with the Chinese diploid,Aconitum volubile and East Asian tetraploids, and 2) a clade of East Asian diploids and Siberian tetraploids. In the former clade, most tetraploid taxa appear to be polyphyletic; sequences from individual plants did not correspond to recognized taxonomic units. This indicates a recent divergence of the East Asian tetraploids.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    International journal of primatology 21 (2000), S. 905-913 
    ISSN: 1573-8604
    Keywords: lemurs ; FISH ; Indridae ; phylogeny ; chromosomal evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We used human-specific probes for chromosomes 3, 11, 14, 15, 17, 19, 21 to paint chromosomes of four species of Indridae (Avahi laniger, Indri indri, Propithecus verreauxi, and P. diadema). All human chromosome probes hybridized specifically to chromosome segments or to whole arms of indrid chromosomes. In general, the results obtained by chromosome painting confirm those obtained by R-banding except for some reciprocal translocations undetected by R-banding. Our findings confirm that in the evolution of the Indridae, Avahi laniger first emerges, then Indri and Propithecus share a common trunk. After populational evolution, this sister clade diverged.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Plant systematics and evolution 224 (2000), S. 127-137 
    ISSN: 1615-6110
    Keywords: Hylocomiaceae ; internal transcribed region 2 ; molecular evolution ; phylogeny ; relative rate test
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nucleotide variation of the internal transcribed spacer 2 region of nuclear ribosomal DNA was used to estimate the phylogeny of the moss family Hylocomiaceae. Both parsimony and neighbour-joining analyses support the monophyly of this family, as well as all seven of the genera within the family.Rhytidium appears as the basal taxon and a North American clade comprisingRhytidiopsis andThelia is indicated. The analyses suggest thatHylocomium s. l., a polyphyletic genus, should be divided into three separate genera. The rate of nucleotide substitutions of the ITS-2 region in the Hylocomiaceae is consistent with the hypothesis of a molecular clock based on a relative rate tests. A nearly constant rate of 6.42 × 10−10 substitutions per site and per year is postulated for the family, a rate close to that of the Winteraceae and slower than that ofLopezia.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    International journal of thermophysics 21 (2000), S. 207-215 
    ISSN: 1572-9567
    Keywords: calorific intensity ; hot probe ; organ or tissue of a living body ; specific heat ; thermal conductivity ; thermal diffusivity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A new method was developed to determine simultaneously the thermal conductivity, thermal diffusivity, specific heat, and calorific intensity of the organ or tissue of a living body either in vivo or in vitro with a thin hot probe. By using the method, the thermophysical properties and calorific intensities of a human palm and in vivo liver and a kidney, heart, brain, and foreleg and hindleg muscles of an anesthetized canine were measured. It is concluded that there are no significant differences in the thermophysical properties of organ or tissue of a living body either in vivo or in vitro. The measured thermophysical properties are in good agreement with those reported in the literature.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    International journal of thermophysics 21 (2000), S. 513-524 
    ISSN: 1572-9567
    Keywords: metal ; microscopic structure ; phase lag ; photoacoustic effect ; thermal diffusivity ; thin layer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The thermal diffusivities of four kinds of metallic foils from 20 to 200μμm in thickness were measured by a photoacoustic method on the basis of the Rosencwaig and Gersho theory. The measured data for continuous foils of uniform microscopic structure almost agreed with the literature values. Measurements were also carried out on two kinds of metallic thin films with of 10μμm thickness produced by sputtering. The difference in thermal diffusivity between the foils and the sputtered films depended on the uniformity of the microscopic structure.
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