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  • Electron microscopy  (61)
  • Scolytidae
  • Springer  (115)
  • 2005-2009
  • 1990-1994  (27)
  • 1985-1989  (21)
  • 1980-1984  (25)
  • 1975-1979  (42)
  • 1965-1969
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  • 1
    Electronic Resource
    Electronic Resource
    Ecdysteroids during pupal development of female Xyleborus ferrugineus (1981)
    Springer
    Entomologia experimentalis et applicata 30 (1981), S. 151-156 
    Details
    ISSN: 1570-7458
    Keywords: Xyleborus ferrugineus ; pupae ; ecdysteroids ; pharate adult ; radioimmunoassay ; Coleoptera ; Scolytidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Der Ecdysteroidtiter weiblicher Puppen von Xyleborus ferrugineus (Fabr.) wurde geschätzt, indem ganze Tiere homogenisiert und radioimmunologisch untersucht wurden. Ein ausgeprägtes Maximum an Ecdysteroiden wurde bei 36 Stunden Puppenent-wicklung beobachtet (743 pg/mg Körpergewicht). Der Titer nahm ab auf 299 pg/mg im Pharatstadium und auf 193 pg/mg unmittelbar vor Schlüpfen der Adulten. Qualitative Studien mit HPLC ergaben in frischen Puppen ein Verhältnis von 3:1 Ecdyson zu 20-Hydrooxyecdyson. Pharatstadien enthielten vor allem 20-Hydrooxyecdyson. Das beobachtete einzige Maximum im Titer stimmt überein mit den Resultaten bei andern untersuchten Coleopteren.
    Notes: Abstract Ecdysteroid titers were estimated on the whole body homogenates of Xyleborus ferrugineus (Fabr.) female pupae during development by radioimmunoassay. A distinct peak of ecdysteroids was observed at 36-hr pupal development (743 pg/mg body wt). Titer declined to 299 pg/mg by the pharate adult stage and to 193 pg/mg body wt just before adult emergence. Qualitative studies by HPLC revealed a ratio of 3:1 ecdysone to 20-hydroxyecdysone in the initial pupal stage. Pharate adults had mainly 20-hydroxyecdysone. The observed single peak in ecdysteroid titer agrees with findings in other studied coleopteran species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00300880
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  • 2
    Electronic Resource
    Electronic Resource
    Diversity of Vicia faba circular mtDNA in whole plants and suspension cultures (1986)
    Springer
    Theoretical and applied genetics 72 (1986), S. 541-547 
    Details
    ISSN: 1432-2242
    Keywords: Mitochondrial DNA of plants ; Electron microscopy ; Suspension culture ; Vicia faba
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A comparative analysis of the Vicia faba mitochondrial genome in whole plants and in longterm suspension culture has been conducted. Restriction fragment patterns of the mtDNA isolated from these two sources were notably different. Electronmicroscopic analysis also revealed significant differences. Large circular mtDNA patterns shifted from a 37–80 kb subpopulation, which was predominant in whole plants, to 18–34 kb subpopulations although in both classes notable quantities of circular molecules of 80 to 120 kb and more were also found. Both in whole plant and suspension culture cells very large circular DNAs were observed. Some of them had lengths nearly 290 kb and could be considered as evidence of the existence of master chromosomes. The minicircular DNA population was also altered. In the suspension culture we observed a notable increase of percentage of minicircles with sizes near 1 kb. Simultaneously, the percentage of minicircles with sizes near 3.5–10 kb significantly increased in suspension culture cells. In addition, a new peak (10–12 kb) of minicircles appeared. Copy number alterations for some sequences homologous to CCC1A, CCC1B and CCC2 (Negruk et al. 1982, 1985) were shown. Southern hybridization revealed the existence of a family of minicircles having sizes 1.4–2 kb with predominance of CCC1A, CCC1B and CCC2. The copy numbers of CCC1B and some minor minicircles was changed in the suspension culture when compared with the whole plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00289538
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  • 3
    Electronic Resource
    Electronic Resource
    The effect of tetrabenazine on the accumulation of noradrenaline in constricted postganglionic sympathetic nerves in vitro (1975)
    Springer
    Cell & tissue research 160 (1975), S. 495-504 
    Details
    ISSN: 1432-0878
    Keywords: Tetrabenazine ; Noradrenaline ; Constricted nerves ; Noradrenaline depletion in vitro ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The accumulation of noradrenaline proximal to a constriction applied to cat hypogastric nerves in vitro has been studied in preparations treated with tetrabenazine. The accumulation of amine was almost completely abolished by the drug. Evidence is presented which suggests that tetrabenazine exerts a direct noradrenaline-depleting effect on the intraneuronal storage vesicles. Recovery of noradrenaline levels after the removal of the drug was rapid and was effected by the biosynthesis of new noradrenaline within the axon.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225766
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  • 4
    Electronic Resource
    Electronic Resource
    Ultrastructure of the theca interna of ovarian follicles in sheep (1978)
    Springer
    Cell & tissue research 187 (1978), S. 457-472 
    Details
    ISSN: 1432-0878
    Keywords: Ovarian follicles (sheep) ; Theca interna ; Oestrogen ; Androgen ; Capillaries ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The theca interna of non-atretic ovarian follicles from 2.0 mm in diameter up to the stage shortly following ovulation was studied by light and electron microscopy. In follicles 〈3.0mm in diameter, the theca interna consisted of about 8–12 layers of flattened cells, together with many capillaries and small bundles of collagen. Two main forms of cellular differentiation were seen. These were towards either fibroblast-like cells or presumed steroidogenic cells whose cytoplasm contained large amounts of predominantly smooth tubular endoplasmic reticulum, to which some ribosomes were attached. The majority of cells were of relatively undifferentiated or intermediate structure. In larger follicles up to the early stages of oestrus the theca interna cells became larger and less flattened, and cells rich in tubular endoplasmic reticulum became proportionately more numerous. By 18 h after the onset of oestrus the theca interna was oedematous, and many cells possessed pseudopodia. Many cells also contained numerous lipid droplets, but there were no signs of thecal cell degeneration or death. Shortly after ovulation the basal lamina of the membrana granulosa was incomplete, and it became more difficult to distinguish between theca and granulosa layers. Structural heterogeneity, with two major cell types and cells of intermediate structure, was present at all stages. It was concluded that: (1) the theca interna of 2.0–2.9 mm follicles contained many cells whose structure was compatible with a steroidogenic capacity; (2) changes in the differentiated thecal cells up to the early stages of oestrus were quantitative rather than qualitative, and suggestive of an increased steroidogenic capacity; (3) the accumulation of lipid in many cells of the theca interna by 18 h after the onset of oestrus probably reflected a reduction in steroidogenic activity; and (4) there was no evidence of any structural specialization to facilitate the transport of steroids from the theca interna to the membrana granulosa.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00229610
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  • 5
    Electronic Resource
    Electronic Resource
    Morphological studies by light and electron microscopy of pancreatic acinar cells under the effect of Tityus serrulatus venom (1994)
    Springer
    Cell & tissue research 278 (1994), S. 255-264 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Scorpion venom ; Exocrine pancreas ; Secretagogue ; Electron microscopy ; Pancreatitis ; cis-Golgi aggregates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. We studied in vivo and in vitro morphological aspects of pancreatic acinar cells after treatment with Tityus serrulatus venom (TSV). After three hours in an in vitro system, positive secretagogue effects of the venom were identifiable both at the light-microscopic (LM) and the electron-microscopic (EM) levels. At 1 μg/ml TSV, maximal secretion (as measured in a concomitant radiolabeling dose-response experiment) of exocrine proteins at 58% was manifest as a discharge of most zymogen granules (ZG) and consequent appearance of secretory material in acinar lumina. At the supramaximal dose of 10 μg/ml TSV, exocytotic images were often observed also with secretory contents previously discharged. The lowest dose of venom at 0.01 μg/ml caused no stimulation of zymogen discharge above resting secretion levels; however, morphological changes were observed. At high doses of TSV, both in vivo and in vitro, large aggregates associated with the cis-Golgi develop between this region and the endoplasmic reticulum (ER). Since Tityus venoms have been associated with causation of pancreatitis, we were interested in comparisons of our experimental tissue with parameters attributed to development of the disease. Our studies have demonstrated considerable evidence that large intracellular vacuoles, discharged ZG, effaced acinar lumina with disappearance of microvilli and other manifestations of possible early events in pancreatitis are indeed frequently observed both in pancreatic lobules in vitro and in whole pancreas in vivo when exposed to TSV.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00414168
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  • 6
    Electronic Resource
    Electronic Resource
    Morphological studies by light and electron microscopy of pancreatic acinar cells under the effect of Tityus serrulatus venom (1994)
    Springer
    Cell & tissue research 278 (1994), S. 255-264 
    Details
    ISSN: 1432-0878
    Keywords: Scorpion venom ; Exocrine pancreas ; Secretagogue ; Electron microscopy ; Pancreatitis ; cis-Golgi aggregates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We studied in vivo and in vitro morphological aspects of pancreatic acinar cells after treatment with Tityus serrulatus venom (TSV). After three hours in an in vitro system, positive secretagogue effects of the venom were identifiable both at the light-microscopic (LM) and the electron-microscopic (EM) levels. At 1 μg/ml TSV, maximal secretion (as measured in a concomitant radiolabeling dose-response experiment) of exocrine proteins at 58% was manifest as a discharge of most zymogen granules (ZG) and consequent appearance of secretory material in acinar lumina. At the supramaximal dose of 10 μg/ml TSV, exocytotic images were often observed also with secretory contents previously discharged. The lowest dose of venom at 0.01 μg/ml caused no stimulation of zymogen discharge above resting secretion levels; however, morphological changes were observed. At high doses of TSV, both in vivo and in vitro, large aggregates associated with the cis-Golgi develop between this region and the endoplasmic reticulum (ER). Since Tityus venoms have been associated with causation of pancreatitis, we were interested in comparisons of our experimental tissue with parameters attributed to development of the disease. Our studies have demonstrated considerable evidence that large intracellular vacuoles, discharged ZG, effaced acinar lumina with disappearance of microvilli and other manifestations of possible early events in pancreatitis are indeed frequently observed both in pancreatic lobules in vitro and in whole pancreas in vivo when exposed to TSV.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00414168
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  • 7
    Electronic Resource
    Electronic Resource
    Aggregation pheromones inDryocoetes affaber (Mann.) (Coleoptera: Scolytidae): stereoisomerism and species specificity (1994)
    Springer
    Journal of chemical ecology 20 (1994), S. 111-124 
    Details
    ISSN: 1573-1561
    Keywords: Semiochemicals pheromones ; Dryocoetes affaber ; Dryocoetes confusus ; Coleoptera ; Scolytidae ; enantiomers ; diastereoisomers ; exo-brevicomin ; endo-brevicomin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Chemical analysis of whole body extracts and volatiles produced by feeding malesDryocoetes affaber (Mann.) disclosed (+)-exo-brevicomin and (+)-endo-brevicomin [(+)EXOB and (+)ENDOB], as the major insect-produced potential pheromones. Laboratory bioassays and field-trapping experiments demonstrated that (+)ENDOB is the main pheromone component, and (-)ENDOB has an inhibiting effect. EXOB either as (+) or (±) appears to be a multifunctional pheromone. It has a synergistic effect in blends of EXOB and ENDOB in ratios up to 1:1, and it is inhibitory at higher ratios. (-)EXOB was inactive. The most attractive blend forD. affaber was a 1:2 blend of (+)EXOB and (+)ENDOB. When this blend was compared with a 9:1 blend, the best known blend forDryocoetes confusus Swaine, the responses by beetles of each of the two species were highly specific, providing evidence for pheromonal exclusion between the two congenerics. We conclude that the combined effect of chirality and the ratio of geometrical isomers of brevicomin determines both the level of response and the species-specificity of the chemical signal inD. affaber.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02065994
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  • 8
    Electronic Resource
    Electronic Resource
    Geometrical and optical isomerism of pheromones in two sympatricDryocoetes species (Coleoptera: Scolytidae), mediates species specificity and response level (1993)
    Springer
    Journal of chemical ecology 19 (1993), S. 2169-2182 
    Details
    ISSN: 1573-1561
    Keywords: Semiochemicals ; pheromones ; Dryocoetes confusus ; Dryocoetes affaber ; Coleoptera ; Scolytidae ; enantiomers ; diastereoisomers ; exo-brevicomin ; endo-brevicomin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In a field-trapping experiment, western balsam bark beetles,Dryocoetes confusus Swaine, were highly attracted to a 5∶1 mixture of (±)-exo-and (±)-endo-brevicomin. Beetles in the sympatric speciesD. affaber (Mann.), were best attracted to a 1∶1 blend of these semiochemicals [either (±)∶(±) or (±)∶(±)], suggesting that both geometrical isomers are pheromone components in these species. In laboratory bioassays and further field experiments, attraction ofD. confusus was greatest when the (+) enantiomers of both geometrical isomers of brevicomin were presented in a 9∶1 ratio. Responses by maleD. confusus to attractive mixtures were reduced in the presence of (−)-exo-brevicomin. Exploitation of the complete range of variability in pheromone structure (both geometrical and optical isomerism) would allow for optimization and regulation of response levels within a species and also could maintain reproductive isolation among sympatric congeneric species primarily through production and response to species-specific blends.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00979655
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  • 9
    Electronic Resource
    Electronic Resource
    Trapping the western pine beetle at and near a source of synthetic attractive pheromone Effects of trap size and position (1979)
    Springer
    Journal of chemical ecology 5 (1979), S. 519-531 
    Details
    ISSN: 1573-1561
    Keywords: Pinus ponderosa ; Dendroctonus brevicomis ; western pine beetle ; attractant ; pheromone ; behavior ; traps ; Coleoptera ; Scolytidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Western pine beetles were caught on unbaited sticky traps placed near a source ofexo-brevicomin, frontalin, and myrcene. Size of trap, distance and direction from the source of attractant, and height from the ground were varied. Significant differences in trap catch were observed in relation to each of the variables. Traps close to the source of attractant caught more beetles than traps farther from the source. Traps downwind of the source of attractant caught more beetles than did upwind traps. More males than females were trapped close to the source of attractant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00987837
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  • 10
    Electronic Resource
    Electronic Resource
    Pheromonal attraction and allomonal interruption ofIps pini in California by the two enantiomers of ipsdienol (1980)
    Springer
    Journal of chemical ecology 6 (1980), S. 703-717 
    Details
    ISSN: 1573-1561
    Keywords: Scolytidae ; bark beetle ; Ips pini ; pheromone ; ipsdienol ; enantiomer ; interruption ; allomone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Air containing volatile compounds from around maleIps pini boring in ponderosa pine logs from California was condensed, fractionated by GC, and assayed in the laboratory and field. The only fraction that showed consistent activity in laboratory assays contained a single compound identified as ipsdienol (2-methyl-6-methylene-2,7-octadien-4-ol). Synthetic racemic ipsdienol showed no activity in either the laboratory or field. However, (−)-ipsdienol, the naturally occurring enantiomer, was attractive toI. pini in the laboratory and field, whereas (+)-ipsdienol interrupted the response ofI. pini to a natural source of attraction in field tests. (−)-Ipsdienol is a major component of the attractant pheromone of this species, since its level of activity in laboratory assays was quantitatively comparable to that of the condensed volatiles, and it was as attractive as maleI. pini boring in ponderosa pine in the field. (+)-Ipsdienol is a component of the pheromone of the competing species,I. paraconfusus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00987680
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