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  • 1
    Publication Date: 1983-02-01
    Print ISSN: 0032-0935
    Electronic ISSN: 1432-2048
    Topics: Biology
    Published by Springer
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  • 2
    ISSN: 1432-2048
    Keywords: DNA (minicircular) ; Mitochondrion ; Vicia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Minicircular DNAs found in mitochondria of 6-d-old etiolated seedlings of Vicia faba L. were also present in mitochondria isolated from cell suspension cultures and from green leaves of this plant. These results support the suggestion that the plasmid-like molecules found in mitochondria of V. faba are an essential component of the mitochondrial genome. The minicircular DNAs were, apparently, peculiar for the species V. faba since they were found in all three cultivars of this species which were studied. The distribution pattern of plasmid-like DNAs in Vicia villosa L. was completely different and mitochondria from Medicago sativa L. also contained specific minicircular DNAs. Thus, minicircular DNAs are typical for the mitochondrial genomes of several legumes and different plant species have their specific mitochondrial plasmid-like DNAs
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2242
    Keywords: Vicia faba ; Cytochrome oxidase subunit II ; Nucleotide sequence ; Chimeric gene ; Transcription
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Southern hybridization analysis of mitochondrial genomes from different lines and cultivars of Vicia faba, with respect to variability of the coxII gene sequence, revealed two predominant types of mitochondrial genomes. The type I mitochondrial genome contained the coxII gene sequence in a 6.5-kb BamHI fragment. Type II had two copies of the coxII sequence: the first in a 6.5-kb and the second in a 2.6-kb BamHI fragment. The second copy was represented by a coxII-orf192 chimeric gene. We found several pure lines with type I and type II mitochondrial genomes. Each type of genome was stably inherited. No chimeric gene was found in mitochondria of the male-sterile line cms447. Nucleotide sequences of Vicia faba mitochondrial DNA (mtDNA) containing normal and chimeric coxII genes are presented. The sequence of the normal coxII gene was compared to the coxII gene from mitochondria of Pisum sativum. The similarity of nucleotide sequences and of predicted amino acid sequences between these two genes was more than 98%. A very high similarity between transcription initiation and termination signals was also observed. The sequence of the chimeric gene was characterized at the 5′ end by the almost complete sequence of the normal coxII gene, up to the fifth nucleotide before the termination codon. The 3′ end of the chimeric gene was represented by the 3′ part of an orf previously called orf128+. The full size of this orf was 576 nucleotides, and the full size of the predicted polypeptide was 192 amino acid residues. Therefore, this orf can be finally called orf192. Northern hybridization analysis showed that orf192 was actively transcribed into a 1.4-kb transcript. The chimeric gene was also transcribed into a minor transcript of about 3 kb. Comparative analysis of the normal coxII gene and orf192 supported the suggestion that the chimeric gene resulted from nonhomologous recombination.
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  • 4
    ISSN: 1432-2242
    Keywords: Mitochondrial DNA of plants ; Electron microscopy ; Suspension culture ; Vicia faba
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A comparative analysis of the Vicia faba mitochondrial genome in whole plants and in longterm suspension culture has been conducted. Restriction fragment patterns of the mtDNA isolated from these two sources were notably different. Electronmicroscopic analysis also revealed significant differences. Large circular mtDNA patterns shifted from a 37–80 kb subpopulation, which was predominant in whole plants, to 18–34 kb subpopulations although in both classes notable quantities of circular molecules of 80 to 120 kb and more were also found. Both in whole plant and suspension culture cells very large circular DNAs were observed. Some of them had lengths nearly 290 kb and could be considered as evidence of the existence of master chromosomes. The minicircular DNA population was also altered. In the suspension culture we observed a notable increase of percentage of minicircles with sizes near 1 kb. Simultaneously, the percentage of minicircles with sizes near 3.5–10 kb significantly increased in suspension culture cells. In addition, a new peak (10–12 kb) of minicircles appeared. Copy number alterations for some sequences homologous to CCC1A, CCC1B and CCC2 (Negruk et al. 1982, 1985) were shown. Southern hybridization revealed the existence of a family of minicircles having sizes 1.4–2 kb with predominance of CCC1A, CCC1B and CCC2. The copy numbers of CCC1B and some minor minicircles was changed in the suspension culture when compared with the whole plants.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 76 (1988), S. 587-592 
    ISSN: 1432-2242
    Keywords: Mitochondrial DNA of plants ; Minicircular ; DNA copy number ; Molecular rearrangements ; Vicia faba
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A comparative analysis of the presence of minicircular DNA CCCIB in 16 different lines and cultivars of fertile Vicia faba L. plants was conducted. It was found that copy number of CCCIB ranged from several copies per mitochondrial genome to — probably — zero, depending on cultivar or line. Fertility of plants in these cases was not altered. We chose 10 cultivars and lines among 16 analysed. Mitochondria of five cultivars and lines contained about two CCCIB molecules per one CCCIA. The sixth cultivar contained CCCIB at copy number several times lower. In the last four cultivars CCCIB could not be identified. Copy number analysis of CCC2 in ten chosen cultivars and lines revealed that in eight cases the quantitiy of CCC2 was equal to CCCIA. However, two other cultivars contained about two times lower quantity of CCC2. Parallel to that we observed an increase in quantity of one sequence homologous to CCC2, which in the first eight cultivars and lines could be found only in minor quantities. Comparative restriction analysis revealed notable rearrangement events in mitochondrial DNAs of ten cultivars and lines being investigated. We did not find any correlations between patterns of restriction fragments and copy number of CCCIB. In some cases, rearrangements in Vicia faba mitochondrial genomes caused a duplication of sequences homologous to the Zea mays coxII gene.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 198 (1985), S. 486-490 
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Three types of minicircular DNA isolated from Vicia faba mitochondria were cloned in pBR322 plasmids. The correspondence of cloned sequences to the original molecules was verified by coelectrophoresis and by DNA-DNA hybridization with total mitochondrial DNA preparations. It was found that the cloned sequences hybridized not only with the minicirclar DNAs and their derivates, but also with some discrete classes of higher molecular weight DNA and with the major DNA. The data obtained from restriction enzyme analysis of complementary sequences suggested that the majority of them were represented by the minicircular DNAs and by the oligomers. The physical maps of cloned sequences were also prepared. These maps differed significantly. However, reciprocal DNA-DNA hybridization showed the existence of sequence homology between minicircular DNAs.
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