ALBERT

Description: The Lena River is one of the biggest Russian rivers draining into the Laptev Sea. Due to predicted increasing temperatures, the permafrost areas surrounding the Lena Delta will melt at increasing rates. With this melting, high amounts of methane will reach the waters of the Lena and the adjacent Laptev Sea. Methane oxidation by methanotrophic bacteria is the only biological way to reduce methane concentrations within the system. However, the polar estuary of the Lena River is a challenging environment for bacteria, with strong fluctuations in salinity and temperature. We determined the activity (tracer method) and the abundance (qPCR) of aerobic methanotrophic bacteria. We described the methanotrophic population with MISA; as well as the methane distribution (head space) and other abiotic parameters in the Lena Delta in September 2013. In riverine water (S 〈 5) we found a median methane concentration of 22 nM, in mixed water (5 〈 S 〈 20) the median methane concentration was 19 nM and in polar water (S 〉 20) a median 28 nM was observed. The Lena River was not the methane source for surface water, and bottom water methane concentrations were mainly influenced by the concentration in surface sediments. However, the methane oxidation rate in riverine and polar water was very similar (0.419 and 0.400 nM/d), but with a higher relative abundance of methanotrophs and a higher estimated diversity with respect to MISA OTUs in the rivine water as compared to polar water. The turnover times of methane ranged from 167 d in mixed water, 91 d in riverine water and only 36 d in polarwater. Also the environmental parameters influencing the methane oxidation rate and the methanotrophic population differed between the water masses. Thus we postulate a riverine methanotrophic population limited by sub-optimal temperatures and substrate concentrations and a polar methanotrophic population being well adapted to the cold and methane poor environment, but limited by the nitrogen content. The diffusive methane flux into the atmosphere ranged from 4–163 µmol m2 d−1 (median 24). For the total methane inventory of the investigated area, the diffusive methane flux was responsible for 8 % loss, compared to only 1 % of the methane consumed by the methanotrophic bacteria within the system.

Description: The Lena River is one of the largest Russian rivers draining into the Laptev Sea. The predicted increases in global temperatures are expected to cause the permafrost areas surrounding the Lena Delta to melt at increasing rates. This melting will result in high amounts of methane reaching the waters of the Lena and the adjacent Laptev Sea. The only biological sink that can lower methane concentrations within this system is methane oxidation by methanotrophic bacteria. However, the polar estuary of the Lena River, due to its strong fluctuations in salinity and temperature, is a challenging environment for bacteria. We determined the activity and abundance of aerobic methanotrophic bacteria by a tracer method and by the quantitative polymerase chain reaction. We described the methanotrophic population with a molecular fingerprinting method (monooxygenase intergenic spacer analysis), as well as the methane distribution (via a headspace method) and other abiotic parameters, in the Lena Delta in September 2013. The median methane concentrations were 22 nmol L−1 for riverine water (salinity (S)  

Description: Large quantities of methane are stored in hydrates and permafrost within shallow marine sediments in the Arctic Ocean. These reservoirs are highly sensitive to climate warming, but the fate of methane released from sediments is uncertain. Here, we review the principal physical and biogeochemical processes that regulate methane fluxes across the seabed, the fate of this methane in the water column, and potential for its release to the atmosphere. We find that, at present, fluxes of dissolved methane are significantly moderated by anaerobic and aerobic oxidation of methane. If methane fluxes increase then a greater proportion of methane will be transported by advection or in the gas phase, which reduces the efficiency of the methanotrophic sink. Higher freshwater discharge to Arctic shelf seas may increase stratification and inhibit transfer of methane gas to surface waters, although there is some evidence that increased stratification may lead to warming of sub-pycnocline waters, increasing the potential for hydrate dissociation. Loss of sea-ice is likely to increase wind speeds and seaair exchange of methane will consequently increase. Studies of the distribution and cycling of methane beneath and within sea ice are limited, but it seems likely that the sea-air methane flux is higher during melting in seasonally ice-covered regions. Our review reveals that increased observations around especially the anaerobic and aerobic oxidation of methane, bubble transport, and the effects of ice cover, are required to fully understand the linkages and feedback pathways between climate warming and release of methane from marine sediments.

Description: River estuaries are responsible for high rates of methane emissions to the atmosphere. The complexity and diversity of estuaries require detailed investigation of methane sources and sinks, as well as of their spatial and seasonal variations. The Elbe river estuary and the adjacent North Sea were chosen as the study site for this survey, which was conducted from October 2010 to June 2012. Using gas chromatography and radiotracer techniques, we measured methane concentrations and methane oxidation (MOX) rates along a 60 km long transect from Cuxhaven to Helgoland. Methane distribution was influenced by input from the methane-rich mouth of the Elbe and gradual dilution by methane-depleted sea water. Methane concentrations near the coast were on average 30 ± 13 nmol L−1, while in the open sea, they were 14 ± 6 nmol L−1. Interestingly, the highest methane concentrations were repeatedly detected near Cuxhaven, not in the Elbe River freshwater end-member as previously reported. Though, we did not find clear seasonality we observed temporal methane variations, which depended on temperature and presumably on water discharge from the Elbe River. The highest MOX rates generally coincided with the highest methane concentrations, and varied from 2.6 ± 2.7 near the coast to 0.417 ± 0.529 nmol L−1 d−1 in the open sea. Turnover times varied from 3 to 〉1000 days. MOX rates were strongly affected by methane concentration, temperature and salinity. We ruled out the supposition that MOX is not an important methane sink in most of the Elbe estuary and adjacent German Bight.

Description: We conducted multiple small (2011–2012) and one large sampling campaign (2013) at selected profiles along the Elbe River. With the data we were able to outline spatial and temporal variability of methane concentration, oxidation and emissions in one of the major rivers of Central Europe. The highest methane concentrations were found in human-altered riverine habitats, i.e., in a harbor (1,888 nmol L−1), in a lock and weirs (1409 ± 1545 nmol L−1), and in general in the whole “impounded” river segment (383 ± 215 nmol L−1). On the other hand, the lowest methane concentrations were found in the “lowland” river segment (86 ± 56 nmol L−1). The methane oxidation rate was more efficient in the “natural” segment (71 ± 113 nmol L−1day−1, which means a turnover time of 49 ± 83 day−1) than in the “lowland” segment (4 ± 3 nmol L−1day−1, which means a turnover time of 39 ± 45 day−1). Methane emissions from the surface water into the atmosphere ranged from 0.4 to 11.9 mg m−2 day−1 (mean 2.1 ± 0.6 mg m−2 day−1) with the highest CH4 emissions at the Meissen harbor (94 kg CH4 year−1). Such human-altered riverine habitats (i.e., harbors and similar) have not been taken into consideration in the CH4 budget before, despite them being part of the river ecosystems, they may be significant CH4 hot-spots. The total CH4 diffusive flux from the whole Elbe was estimated to be approximately 97 t CH4 year−1.

Description: Large amounts of the greenhouse gas methane are released from the seabed but liberation of methane to the atmosphere is mitigated by aerobic methanotrophs in the water column. The size and activity of methanotrophic communities are thought to be mainly determined by nutrient and redox dynamics, but little is known about the effects of water mass transport. Here, we show that cold bottom waters at methane seeps west off Svalbard, which contained a large number of aerobic methanotrophs, were rapidly displaced by warmer waters with a considerably smaller methanotrophic community. This water mass exchange, caused by short-term variations of the West Spitsbergen Current strongly reduced methanotrophic activity. Currents are common at many methane seeps and could thus be a globally important control on methane oxidation in the water column.

Description: Rivers represent a transition zone between terrestric and aquatic environments, and between methane rich and methane poor environments. The Elbe River is one of the most important rivers draining into the North Sea and, along with the Elbe, a potential importer of high amounts of methane into the water column of the North Sea. Twelve sampling cruises from October 2010 until June 2013 were conducted from Hamburg towards the mouth of the Elbe at Cuxhaven. The dynamic of methane concentration in the water column and its consumption via methane oxidation was measured. In addition, physico-chemical parameters were used to estimate their influence on the methanotrophic activity. We observed high methane concentrations at the stations in the area of Hamburg harbor (“inner estuary”) and about 10 times lower concentrations in the outer estuary (median of 416 versus 40 nmol/L, respectively). The methane oxidation (MOX) rate mirrored the methane distribution with high values in the inner estuary and low values in the outer estuary (median of 161 versus 10 nmol/L/d, respectively). Methane concentrations were significantly influenced by the river hydrology (falling water level) and the trophic state of the water (biological oxygen demand). In contrast to other studies no clear relation to the amount of suspended particulate matter (SPM) was found. Methane oxidation rates were significantly influenced by methane concentration and to a lesser extent by temperature. Methane oxidation accounted for 41 ± 12% of the total loss of methane in the summer/fall, but for only 5 ± 3% of the total loss in the winter/spring. We applied a modified box model taking into account the residence times of each water parcel depending on discharge and tidal impact. We observed almost stable methane concentrations in the outer estuary, despite a strong loss of methane through diffusion and oxidation. Thus, we postulate that the water column undergoes a balancing out in the outer Elbe estuary due to a strong additional input of methane, which could be provided by the extensive salt marshes near the river mouth.