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  • 1
    Publication Date: 2021-05-19
    Description: In order to domesticate Litopenaeus vannamei as a solution to single species shrimp aquaculture of Penaeus indicus and to reduce risk of shrimp culture in emergency cases, this study was carried out. Initially the postlarvae reared from imported L. vannamei were cultured. After culture season the adult shrimps were transported to greenhouse ponds for wintering. It is then relocated to hatchery saloon. After eye stalk ablation and maturation the female brood stocks were introduced to matured male for copulation at ponds. During the study period, male and female shrimps attained full maturation at end – April by temperature over 25ºC, the copulation process was started significantly. Finally of the 12 instances of copulation, one instance resulted nauplii production. The best maturation was observed at 28 ֯C ±1 and salinity30 to 33PPT and pH of 8 to 8.3 Fresh sea worms and squid showed the highest effect on fecundity in brood stocks. Besides, the spring season was obtained to be the best period for brood stock production in Bushehr province.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Litopenaeus vannamei ; Brood stocks ; Larvae ; Species ; Shrimp ; Aquaculture ; Postlarvae ; L.vannamei ; Hatchery ; Maturation ; Nauplii ; pH ; Sea worm ; Squid
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 25pp.
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  • 2
    Publication Date: 2021-05-19
    Description: For the successful breeding of western white shrimps (Litopenaeus vannamei) brooders (females and males), we used cuttle fish (Sepia pharaonis) and sand worm (Perinereis nuntica) of Persian Gulf as principle feeds, 3 times a day, and from cow livier and edible bivalve meat (Solen brevis) of Persian Gulf, as secondary feeds, 1 time a day. Nutritional value of Sepia pharaonis, Perinereis nuntica and Solen brevis, with determination of the amount of fatty acids such as arachidonic acid (20:4n-6), stearic acid (18:00), oleic acid (18:1n-9), palmitic acid, palmitoleic acid (16:1n-7), eicosapentaenoic acid (20:5n-3) and docosahexaenoic acid, essential amino acids inclusive arginine, lysine, leucine, isoleusine, threonine, valine, histidine, phenylalanine, methionine, tryptophane and crude protein, crude fat, crude fiber, ash and moisture were done in laboratory. The results showed that, the amount of essential amino acides and nonessential amino acides (in the more causes) in Sepia pharaonis were more than Solen brevis and in Solen brevis more than Perinereis nuntica. Only nonessential amino acides, glysine and alanine in Solen brevis were more than Sepia pharaonis. Also in chemical analysis of wet feeds determined that, crude protein percent in cattle fish was 2.9 times and 2.2 times more than Solen brevis and Perinereis nuntica, respectively. The crude fat in cattle fish was 2.1 times and 1.6 times more than Solen brevis and Perinereis nuntica respectively. Tottaly, the amount of essential fatty acides in Sepia pharaonis and Perinereis nuntica were more than Solen brevis, and in Sepia pharaonis and Perinereis nuntica were almost, equal. Total lipids of Sepia pharaonis, Perinereis nuntica and Solen brevis determined 7.92, 5.90 and 3.63 percent respectively. Totally the percent of fatty acids in cattle fish and Perinereis nuntica is almost equal and was more than Solen brevis meat. We can noted that, the Sepia pharaonis and Perinereis nuntica are suitable as principle feed and Solen brevis and cow livier, as secondary feed for L.vannamei broodstock maturation.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Broodstock ; Western white shrimp ; Wet and Fresh Feed ; Maturation ; Litopenaeus vannamei ; Females ; Males ; Cuttle fish ; Sepia pharaonis ; Sand worm ; Perinereis nuntica ; Solen brevis ; Perinereis nuntica ; Fatty acids ; Liver
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 36pp.
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  • 3
    Publication Date: 2021-05-19
    Description: In this project,four types of structures,of 40 number was investigated.In a six month period,the structures were inspected 13 times.The total weight and number of eggs hatched on the structures were estimated to be 214.1kg and 94944 respectively.The weight and number of eggs settled on trap types were 141.9 and 60982 respectively,whereas,The eggs on cubical structures were estimated 56.7kg and 27088 ,on pyramid structures 7.9 and 3941 and then also were 6.9kg and 3280 eggs on cylindrical structures respectively.It is also estimated that 358 eggs were settled on The connecting line weighting 0.5kg.In this report four types of structures was compared.As a result,the trap, cubical, pyramid and cylindrical structures had contributed to the settelment of 66,27,4 and 3 percent of eggs in weight. While 64,29,4 and 3 percent of eggs in number was settled on these structures respectively.Thus it is concluded that the trap structures fallowed by cubical ones had acted more effectively relative to other two.If based on the results of other studies,we assume 80% survival rate of eggs and average weight of 1.2kg of survival growth cuttlefish , then it can be calculated that 160 structures can contribute to 91 tons increase in cuttlefish stock in one area of 4 to 5 acre.It is obviaus that by planning an annual increase of the structures,it is passible to attract more spawners to spawn and produce more larvae.Moreover,The old structures will be covered by corals and barnacles and become amore stalie and attractive spawning ground for cuttlefish.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Spawning ; Cuttlefish ; Structures ; Trap ; Growth ; Produce ; Types ; Weight ; Eggs ; Survival rate
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 66pp.
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  • 4
    Publication Date: 2021-05-19
    Description: The effects of aquaculture industry on the environment were evaluated by studying the water quality of Bushehr costal water during culture season 2005 and 2006. The variations of selected parameters such as total phosphorus, ammonia, chlorophyll a, pH, salinity and... Were monitored in effluent canal, influent canal and open sea in Helleh and Mond regions as monthly. Following occurrence of White Spot Disease (W.S.D) in the shrimp ponds of Bushehr province, shrimp culture suspended and entrance of effluent waters completely were blocked since August 2005, despite the absence of aquaculture sewage evaluation of environment and sampling were continued. During releasing of sewage of shrimp ponds to the coastal waters of Bushehr in Jun 2005, the average amount of ammonia in Helleh and Mond were obtained to be 0.161 mg/l and 0.194 mg/l, however the average amount of total phosphorus in Helleh and Mond regions were 0.149 mg/l and 0.043 mg/l, respectively. Although effluent water suspension, amount of ammonia and total phosphorus were increased in both region especially in June 2006. Comparing present data in Jun 2005 and recorded data from culture period 1997-2003 with permitable range of municipal and aquaculture waste show that; aquaculture industry on both regions have not had any obvious negative effect on the coastal waters of Bushehr but it seems if other sources of pollutions in the region is not controlled, then the aquaculture industry can contribute to occurrence of environmental problems.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Environmental ; Survey ; Shrimp ; Aquaculture ; Phosphorus ; Ammonia ; Chlorophyll a ; pH ; Salinity ; White spot disease ; Sampling ; Pollution ; Coastal waters
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 73pp.
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  • 5
    Publication Date: 2021-05-19
    Description: We determined the LC50 of copper (as CUSO4) and it's Maximum Allowable Concentration (MAC) for Litopenaeus vannamei. The study was performed in 2003 for which bioassays were used for acute toxicity tests in a period of 96 hours during which water parameters such as water temperature, pH, dissolved oxygen, hardness, alkalinity were also measured. Ten treatments and three replicates for each treatment were used. A LC50 of 86.71, 27.28, 7.98 and 3.90mgi1 and also Maximum Allowable Concentration of 8.671, 2.728, 0.798 and 0.390mgi1 were determined at 24, 48, 72 and 96 hours post exposure. Results showed that L. vannamei is relatively resistant to copper. Hence, short period application of copper to white shrimp farms for controlling algal bloom is judged harmless to the fish.
    Description: Published
    Keywords: LC50 ; Litopenaeus vannamei ; Copper Sulphate ; Fish
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.103-110
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  • 6
    Publication Date: 2021-05-19
    Description: At present, the aquaculture industry to provide proper instructions in the field of health management, including production of Specific Pathogen Free shrimp (SPF), require sensitive and reliable methods for the detection and identification of pathogenic microorganisms. Molecular methods which used in the detection of microorganisms have a high discriminatory power in the taxonomy and in relation to libraries in the world. On the other hand, the accurate identification of microorganisms, providing the genetic data bank of shrimp pathogens and maintenance of these strains is the step to promote further research on the mechanisms of pathogenesis of pathogens, diagnosis, treatment, prevention of disease, identify indigenous production kits, diagnosis re emerging and emerging diseases and their origin. Therefore, in this project, by using ribotyping technique, native isolated pathogenic bacteria and fungi were identified and recorded in the gene bank database center. During sampling of shrimp and water of Specific Pathogen Free shrimp center, 40 bacterial strains were isolated, which 8 of them had the most frequency and identification based on 16S rDNA sequencing was performed. Bacteria identified are: Vibrio nigripulchritudo strain IS013(GenBank:KP843725), Vibrio brasiliensis strain IS014 (GenBank:KR186076), Vibrio rotiferianus strain IS015 (GenBank:KR186077), Vibrio azureus strain IS012 (GenBank:KJ018724.1), Vibrio owensii strain IS016 (GenBank:KR186078), Agarivorans gilvus strain IS017 (GenBank:KR186079), Vibrio brasiliensis IS018 (GenBank:KR186080) and Vibrio alginolyticus strain IS019 (GenBank:1817854), which were recorded in The World Bank genes. In this study fungal isolates were not detected.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Bacteria ; Fungi ; Ribotyping ; 16S rDNA ; Shrimp ; Specific pathogen free ; Vibrio rotiferianus ; Vibrio owensii ; Vibrio brasiliensis ; Vibrio azureus ; Agarivorans gilvus ; Vibrio alginolyticus ; GenBank
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 64pp.
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  • 7
    Publication Date: 2021-05-19
    Description: The influence of different dietary levels of plant protein (30, 50 and 70%) on growth indices of White Leg Shrimp (Litopenaeus vannamei) fed with an original 38 percent protein was assessed and compared with that of the commercial shrimp diet. The average weight gain at the end of the culture period in treatment 1 (30% plant protein and 70% animal protein), was 5.89 plus or minus 0.06 grams, for treatment 2 (50% plant protein and 50% animal protein), was 6.22 plus or minus 0.25 grams, for treatment 3 (70% plant protein and 30% animal protein), was 6.19 plus or minus 0.24 grams and for the control treatment (20% plant protein and 80% animal protein), was 6.42 plus or minus 0.40 grams. Our study of the influence of experimental and control diets on growth indices including growth rate (GR), food conversion ratio (FCR), protein efficiency ratio (PER), specific growth rate (SGR), and average daily gain (ADG), demonstrated that the indices were better in the control compared to the treatments. No significant difference was found among the treatments and between the treatments and the control for the indices (P〉0.05). The percent of net protein utilization (NPU) in treatment 1 was significantly more than other treatments (P0.05). We also found that the application of diets containing 50% plant protein (with 20% soybean meal), and 70% plant protein (with 41.72% soybean meal), can decrease diet costs. Cost of one kilogram of pellet, in treatments 1, 2, 3 were, 83.3%, 68.3% and 53.3% lower than the control diet respectively. Decrease of cost in treatment 2 and 3, in comparison to control in terms of lowering shrimp production cost is very important.
    Description: Published
    Keywords: Shrimp culture ; Litopenaeus vannamei ; Growth ; Weight ; Protein deficiency ; Dietary deficiencies ; Feeding experiments ; Feed composition ; Marine
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.79-88
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  • 8
    Publication Date: 2021-05-19
    Description: Beacaus of increasing genetic variation and avoid of future hazard, monoculture of Iran native shrimp species , penaeus semisulcatus (green tigershrimp) is considered. One of the most important problems in monoculture is detection relationshipbetween biological and nonbiological factors, and thier effects on growth and survival of species.This study was caried out in four ponds (four ponds 0.4 ha). According to results, this species of shrimp needs to quantiative foods , otherwise it caues canibalism, abnormal distribution, in weight distribution diagram of shrimp and reduction of production in squere unit. Amongindependent factors such us water temprature, salinity, PH, transparency,water deep and food consumption rate, four first factors have the mostcorrelation by shrimp weight (r2=0.922).Shrimps weight variation in 3 Range,25-28, 28-31and more them 31 degree centigrade were studied. There wassignificance difference in shrimp rate variation in 3 ranges which werementioned (P〈0.05). Shrimp had best growth in 25-28 degree centigrade.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Penaeus semisulcatus ; Factor physico-chemical ; Green tiger shrimp ; Species ; Temperature ; Weight ; Bionormative ; Culture
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 36pp.
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  • 9
    Publication Date: 2021-05-19
    Description: In order to investigate some ecological condition of the waters of Bushehr province in the Persian gulf between 50~'-01 E to 52~'-47 E and 26~'-39 N to 29~'-03 N , 18 major and 16 minor stations have been studied from Nayband bay to Kharg Island, using R.V. Ferdows 1. There have been a preliminary survey in winter 2001, 4 seasonal surveys in 2001-2 and a complementary one in summer 2002. During this period the seasonal environmental conditions, physico-chemical parameters, zoo and phytoplankton concentrations in water layers, macrobenthoses and sediments were studied. Sea bed swelling in the Motaf region has resulted in division of the area in two parts and in a such away that the climatic and chemico-physical conditions in the southeast area are different from the northwest area. In the southeast area the termocline, halocline, picnocline and oxycline are generated in spring, increased in summer, moved down to the deeper layers in fall and disappeared in the winter. In the Northwest area the clines are only formed in spring and summer but disappear in fall and winter due to almost perfect water column mixing resulted from climatological conditions. During survey period the water temperature ranged from 18 34.1~'C, salinity 37.9 41.3 ppt, conductivity 51.3 70.4 ms/cm, sigma〈sub〉T〈/sub〉 23.1 30.2 g/cm〈super〉3〈/super〉, dissolved oxygen 0.2 8 ppm, and chlorophyll a 0.1 3.6 mg/m〈super〉3〈/super〉. It is observed that salinity, conductivity and sigma〈sub〉T〈/sub〉 have increased from surface to depeer layer, while oxygen and chlorophyll a had a pick in mean layers but temperature have decreased from surface to the depth. Temperature and conductivity decreased from southeast to the northwest and from costal to offshore. Salinity and sigma〈sub〉T〈/sub〉 increased from southeast to the northwest. Dissolved oxygen and chlorophyll-a did not show any trend. The concentration of nutrients including ortho silicate (Si-SiO〈sub〉4〈/sub〉〈super〉- 4〈/super〉), ortho phosphosphate (P PO〈sub〉4〈/sub〉〈super〉-4〈/super〉), nitrate (N NO〈sub〉3〈/sub〉〈super〉-〈/super〉 ) and nitrite (N NO〈sub〉2〈/sub〉〈super〉-〈/super〉 ) varied between 0.4 13.3, 0.05 1.6, 0.1 12.3 and 0.1 1.4 ~kmol/l, respectively. The overall nutrient concentrations increased from surface to depth. 61 genuses of 5 phytoplankton groups and 8 branches, 12 classes of zooplankton were identified. The identified phytoplanktons included 42 Diatom, 16 Dinophyceae, 3 Cyanophyceae, one Chrysophyceae and one Euglenaphyceae geneses. The highest intensity of phytoplanktons was recorded in summer, while in the case of zooplankton, was recorded in spring. Diatoms had the highest variety in all seasons and the maximum abundance in fall and winter. Cyanophyceaes were predominant group in the spring and summer. On the contrary to zooplanktons, the intensity of phytoplanktons decreased from coastal to the offshore waters. The highest intensity of all planktons (zoo and phyto) was in 10-20 m layer. The intensity of phyto plankton increased from southeast towards northwest. The zooplankton concentration didn t follow any particular trend. The identified macrobenthoses were 69 families including 34 polychaets, 22 gastropods, 10 of bivalvs, 1 crustacean, 1 scaphopoda, 1 foraminifera, 5 classes and 6 orders of crustacea, 1 order of echinodermata, 1 fish larva and 6 branches of other benthoses. Intensity and biomass of all groups were determined. The polychaets and malacostraca had the most abundance in contrast to the other groups. Macrobenthosis intensity decreased from coast to the offshore in southeast of Motaf region, while the middle stations of northwest of Motaf had a better intensity. The mean abundance and biomass of total area decreased from coast to the offshore, and the slope of decrease in biomass was more than abundance. Seasonal minimum and maximum abundance ranged from 1457 to 1903 per square meter in spring and winter, and the biomass ranged between 7.8-10.8 g/m〈super〉2〈/super〉 in summer and spring, respectively. In spite of high abundance of macrobenthose in the transect no: 13, the estimated biomass was lower than other transects. This might be due to prolonged exposition to more pollutant. The grain size of sediments was classified as gravel, sand, silt and clay. The organic mater of sediments ranged from 1.12 3.3% with annual average of 1.6%. The grain size of sediments in southeastern point was coarser than other areas. The middle stations of northwestern area had fine grain and more organic mater in contrast to the offshore and coastal stations.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Hydrobiological ; Ecological ; Environmental ; Physico-chemical ; Hydrology ; Sturgeon ; Survey ; Phytoplanktons ; Macrobenthose ; Sediments
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 216pp.
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  • 10
    Publication Date: 2021-05-19
    Description: This study has been carried out in waters of Bushehr province in the Persian Gulf covering Nayband bay to Bahregansar between 26° 45 N to 30° 00 N and 49° 45 E to 52° 45 E, 15 main and 5 sub- stations, by R/V Ferdows 1 in winter 2004, summer 2004 and winter 2005. The physico - chemical parameters, nutrients (silicate, phosphate, nitrate and nitrite), phyto, zooplanktons, sediments and macrobenthoses were collected from water layers and sea bottom, and then analysed. The parameters were compared and evaluated with the results of the previous work, conducted in the same area. Termocline, halocline, oxycline and picnocline layers were formed in summer. These clins disappeared due to water column mixing in winter. The values of temperature, conductivity and pH in summer were higher than those of in winter, while the salinity and density values of surface and subsurface layers in winter were higher than those of in summer. The temperature and conductivity have been decreased from surface to depth, from coastal rigions to offshore and from southern waters to northwern waters, while salinity, density and turbidity have been increased. The dissolved oxygen is decreased by increasing the values of temperature, salinity and depth and it is observed that D.O. has increased in correlation with chlorophyl a in mean and subsurface layers. The pH was rather decreased from surface to depth while turbidity was increased. Orthosilicate (Si_SiO44), nitrate (N_NO3 ), and nitrite (N_NO2 ) concentration have increased from surface to depth while orthophostate (P_PO43) decreased. Among the phytoplanktons, Dinophycea had most abundance in winter 2004 and Diatoms had most abundance, variety and distribution in summer 2004 and winter 2005. Among the zooplanktons, Copeopda had most abundance, variety and distribution. The intensity of phytoplanktons in summer was more than those in winter; it decreased from surface to depth and from coastal rigions to offshore, at first increased and then gradually decreases. Intensity of zooplanktons in summer was more than those in winter and decreased from surface layers to depth and from coastal waters to offshore. The abundance of benthoses in summer was more than that in winter while their biomass in winter was more than that in summer. The abundance decreased from southern to northwern waters, but the rate of decrease in biomass was more than that of abundance. The mainly texture of sediments was loamy or muddy and the grain size in southeastern and northwestern areas were coarser than those in middle area. Compared with the previous study, the trend of physico chemical, silicate, nitrate and nitrite were the same but in spite of the past, the values of pH in summer were more than those in winter and also phosphate concentration had been decreased frome surface layer to depth. The overall trends of phyto and zooplanktons densities are almost similar to previous observation, but cyanophyceae significantly decreased, in comparison with spring and summer abundance. It is also observed that the zooplanktons average density has been in reverse order compared to previous observations. Average concentration of phosphate in the entire region was seven times more than that of the former study and its maximum value has rised more than 26 times in the petroleum and gas industries areas. On the other hand, the abundance and diversity of phyto and zooplanktons and also the ratio of biomass to abundance of benthoses were decreased.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Chemical ; Physicochemical ; Hydrology ; Hydrobiology ; Nutrients ; Phytoplankton ; Zooplankton ; Sediments ; Macrobenthose ; Temperature ; Dissolved oxygen ; Chlorophyll a ; pH ; Diatoms ; Copeopda ; Depth ; Monitoring
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 177pp.
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