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  • 1
    Publikationsdatum: 2002-07-20
    Beschreibung: Mammalian organogenesis requires the expansion of pluripotent precursor cells before the subsequent determination of specific cell types, but the tissue-specific molecular mechanisms that regulate the initial expansion of primordial cells remain poorly defined. We have genetically established that Six6 homeodomain factor, acting as a strong tissue-specific repressor, regulates early progenitor cell proliferation during mammalian retinogenesis and pituitary development. Six6, in association with Dach corepressors, regulates proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter. These data reveal a molecular mechanism by which a tissue-specific transcriptional repressor-corepressor complex can provide an organ-specific strategy for physiological expansion of precursor populations.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Li, Xue -- Perissi, Valentina -- Liu, Forrest -- Rose, David W -- Rosenfeld, Michael G -- 484/B/Telethon/Italy -- 5F32DK09814/DK/NIDDK NIH HHS/ -- New York, N.Y. -- Science. 2002 Aug 16;297(5584):1180-3. Epub 2002 Jul 18.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Howard Hughes Medical Institute, Department of Molecular Medicine, University of California, San Diego, School of Medicine, 9500 Gilman Drive, Room 345, La Jolla, CA 92093-0648, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12130660" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Apoptosis ; Cell Cycle ; Cell Cycle Proteins/genetics/metabolism ; *Cell Division ; Cell Line ; Cyclin-Dependent Kinase Inhibitor p27 ; Cyclin-Dependent Kinases/antagonists & inhibitors ; Embryo, Mammalian/cytology ; Eye Proteins/metabolism ; Homeodomain Proteins/*genetics/*metabolism ; Mice ; Nuclear Proteins/metabolism ; Organ Specificity ; Pituitary Gland/*cytology/embryology ; Promoter Regions, Genetic ; Proto-Oncogene Proteins/genetics/metabolism ; Recombinant Fusion Proteins/metabolism ; Repressor Proteins/metabolism ; Retina/*cytology/embryology ; Retinal Ganglion Cells/cytology/physiology ; Stem Cells/*physiology ; Trans-Activators/*genetics/*metabolism ; Transcription Factors ; Transcription, Genetic ; Transfection ; Tumor Suppressor Proteins/genetics/metabolism ; Up-Regulation
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Publikationsdatum: 2002-04-06
    Beschreibung: We have produced a draft sequence of the rice genome for the most widely cultivated subspecies in China, Oryza sativa L. ssp. indica, by whole-genome shotgun sequencing. The genome was 466 megabases in size, with an estimated 46,022 to 55,615 genes. Functional coverage in the assembled sequences was 92.0%. About 42.2% of the genome was in exact 20-nucleotide oligomer repeats, and most of the transposons were in the intergenic regions between genes. Although 80.6% of predicted Arabidopsis thaliana genes had a homolog in rice, only 49.4% of predicted rice genes had a homolog in A. thaliana. The large proportion of rice genes with no recognizable homologs is due to a gradient in the GC content of rice coding sequences.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Yu, Jun -- Hu, Songnian -- Wang, Jun -- Wong, Gane Ka-Shu -- Li, Songgang -- Liu, Bin -- Deng, Yajun -- Dai, Li -- Zhou, Yan -- Zhang, Xiuqing -- Cao, Mengliang -- Liu, Jing -- Sun, Jiandong -- Tang, Jiabin -- Chen, Yanjiong -- Huang, Xiaobing -- Lin, Wei -- Ye, Chen -- Tong, Wei -- Cong, Lijuan -- Geng, Jianing -- Han, Yujun -- Li, Lin -- Li, Wei -- Hu, Guangqiang -- Huang, Xiangang -- Li, Wenjie -- Li, Jian -- Liu, Zhanwei -- Li, Long -- Liu, Jianping -- Qi, Qiuhui -- Liu, Jinsong -- Li, Li -- Li, Tao -- Wang, Xuegang -- Lu, Hong -- Wu, Tingting -- Zhu, Miao -- Ni, Peixiang -- Han, Hua -- Dong, Wei -- Ren, Xiaoyu -- Feng, Xiaoli -- Cui, Peng -- Li, Xianran -- Wang, Hao -- Xu, Xin -- Zhai, Wenxue -- Xu, Zhao -- Zhang, Jinsong -- He, Sijie -- Zhang, Jianguo -- Xu, Jichen -- Zhang, Kunlin -- Zheng, Xianwu -- Dong, Jianhai -- Zeng, Wanyong -- Tao, Lin -- Ye, Jia -- Tan, Jun -- Ren, Xide -- Chen, Xuewei -- He, Jun -- Liu, Daofeng -- Tian, Wei -- Tian, Chaoguang -- Xia, Hongai -- Bao, Qiyu -- Li, Gang -- Gao, Hui -- Cao, Ting -- Wang, Juan -- Zhao, Wenming -- Li, Ping -- Chen, Wei -- Wang, Xudong -- Zhang, Yong -- Hu, Jianfei -- Wang, Jing -- Liu, Song -- Yang, Jian -- Zhang, Guangyu -- Xiong, Yuqing -- Li, Zhijie -- Mao, Long -- Zhou, Chengshu -- Zhu, Zhen -- Chen, Runsheng -- Hao, Bailin -- Zheng, Weimou -- Chen, Shouyi -- Guo, Wei -- Li, Guojie -- Liu, Siqi -- Tao, Ming -- Wang, Jian -- Zhu, Lihuang -- Yuan, Longping -- Yang, Huanming -- 1 RO1 ES09909/ES/NIEHS NIH HHS/ -- New York, N.Y. -- Science. 2002 Apr 5;296(5565):79-92.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Beijing Genomics Institute/Center of Genomics and Bioinformatics, Chinese Academy of Sciences, Beijing 101300, China.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11935017" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Arabidopsis/genetics ; Base Composition ; Computational Biology ; Contig Mapping ; DNA Transposable Elements ; DNA, Intergenic ; DNA, Plant/chemistry/genetics ; Databases, Nucleic Acid ; Exons ; Gene Duplication ; Genes, Plant ; *Genome, Plant ; Genomics ; Introns ; Molecular Sequence Data ; Oryza/*genetics ; Plant Proteins/chemistry/genetics ; Polymorphism, Genetic ; Repetitive Sequences, Nucleic Acid ; *Sequence Analysis, DNA ; Sequence Homology, Nucleic Acid ; Software ; Species Specificity ; Synteny
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
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    American Association for the Advancement of Science (AAAS)
    Publikationsdatum: 2002-05-23
    Beschreibung: RNA silencing is a sequence-specific RNA degradation mechanism that is operational in plants and animals. Here, we show that flock house virus (FHV) is both an initiator and a target of RNA silencing in Drosophila host cells and that FHV infection requires suppression of RNA silencing by an FHV-encoded protein, B2. These findings establish RNA silencing as an adaptive antiviral defense in animal cells. B2 also inhibits RNA silencing in transgenic plants, providing evidence for a conserved RNA silencing pathway in the plant and animal kingdoms.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Li, Hongwei -- Li, Wan Xiang -- Ding, Shou Wei -- New York, N.Y. -- Science. 2002 May 17;296(5571):1319-21.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Plant Pathology and Center for Plant Cell Biology, University of California, Riverside, CA 92521, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12016316" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Agrobacterium tumefaciens/genetics ; Animals ; Cell Line ; Drosophila/genetics/*virology ; *Gene Silencing ; Genes, Viral ; Green Fluorescent Proteins ; Luminescent Proteins/genetics ; Nodaviridae/*genetics/*physiology ; Plant Leaves/genetics/metabolism ; Plants, Genetically Modified ; RNA, Double-Stranded/genetics/metabolism ; RNA, Small Interfering ; RNA, Untranslated/*metabolism ; RNA, Viral/genetics/metabolism ; Tobacco/*genetics/metabolism/microbiology ; Transfection ; Viral Proteins/genetics/*physiology
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Publikationsdatum: 2002-04-01
    Print ISSN: 0178-2762
    Digitale ISSN: 1432-0789
    Thema: Biologie , Geologie und Paläontologie , Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Publiziert von Springer
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 91 (2002), S. 3067-3073 
    ISSN: 1089-7550
    Quelle: AIP Digital Archive
    Thema: Physik
    Notizen: We report on improved InAs/InAs1−xSbx heterostructure single quantum well emitters for the mid-infrared wavelength region. An InAlAs barrier layer has been incorporated into the active region of the structures for improved electron confinement. With room temperature pulsed power outputs of 140 μW at a wavelength of 4.3 μm, an improvement of more than a factor of 6 has been achieved compared to similar structures without the barrier layer. Magneto-electroluminescence measurements at 4 K reveal the presence of multiple subband transitions in the spectra. Based on a k⋅p model assuming a type-IIa band offset, these transitions are identified as (e1,hh1) and (e1,lh1). The observation of these multiple transitions is shown to be strong evidence for population inversion in the structures. © 2002 American Institute of Physics.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    Digitale Medien
    Digitale Medien
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 92 (2002), S. 1406-1410 
    ISSN: 1089-7550
    Quelle: AIP Digital Archive
    Thema: Physik
    Notizen: La0.67Ca0.33Mn1−xCuxO3 (x=0 and 0.15) epitaxial thin films were grown on the (100) LaAlO3 substrates, and the temperature dependence of their resistivity was measured in magnetic fields up to 12 T by a four-probe technique. We found that the competition between the ferromagnetic metallic (FM) and paramagnetic insulating (PI) phases plays an important role in the observed colossal magnetoresistance (CMR) effect. Based on a scenario that the doped manganites approximately consist of phase-separated FM and PI regions, a simple phenomenological model was proposed to describe the CMR effect. Using this model, we calculated the resistivity as functions of temperature and magnetic field. The model not only qualitatively accounts for some main features related to the CMR effect, but also quantitatively agrees with the experimental observations. © 2002 American Institute of Physics.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    Digitale Medien
    Digitale Medien
    Oxford, UK : Blackwell Publishing Ltd
    Journal of rapid methods and automation in microbiology 10 (2002), S. 0 
    ISSN: 1745-4581
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: The objective of this study was to construct an insertion mutation in the lysA gene of Escherichia coli and investigate the effect of the insertion mutation on the growth rate response. The lysA gene encodes the last enzyme in the lysine biosynthetic pathway in most bacteria. A suicide plasmid pXL1 carrying a segment of the lysA sequence was transformed into E. coli SM10 that is lysogenic for λ pir. The plasmid was extracted from the transformant and confirmed to contain the segment of lysA by restriction enzyme digestion. After this confirmation, pXL1 was transferred by conjugation to an E. coli strain that cannot support replication of the plasmid. Maintenance of the selectable drug marker on the plasmid requires that the plasmid integrate into the chromosome at the lysA locus. The constructed mutant could grow in the absence of lysine supplementation although its growth rate was significantly (P 〈 0.05) depressed when compared to the parent strain.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    Digitale Medien
    Digitale Medien
    Oxford, UK : Blackwell Publishing Ltd
    Journal of rapid methods and automation in microbiology 10 (2002), S. 0 
    ISSN: 1745-4581
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: Optimizing economic utilization of feed protein sources for poultry nutritional requiremens is difficult to achieve given the varied protein quality of the respective sources. Although there are several limiting amino acids in feeds that would benefit from development of rapid and more reliable bioavailability assays, lysine is of key importance since this amino acid is usually the first or second limiting amino acid in poultry feeds and is susceptible to processing treatments. However, to incorporate incoming sources in the most cost-effective manner, accurate and timely prediction of lysine bioavailability prior to use is desired to achieve a consistent nutritional value. Animal bioassays involving chicks are one of the standard accepted practices for evaluating protein quality and amino acid bioavailability, but such assays have several limitations. Alternative in vitro tests that accurately predict lysine bioavailability for feed proteins would solve many of the problems associated with currently used animal bioassays. The expected focus should be on the development of more rapid in vitro lysine bioavailability assays that could be easily used for evaluation of poultry feed protein sources.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 9
    ISSN: 1089-7623
    Quelle: AIP Digital Archive
    Thema: Physik , Elektrotechnik, Elektronik, Nachrichtentechnik
    Notizen: A new electron cyclotron resonance (ECR) ion source (LECR3—Lanzhou Electron Cyclotron Resonance Ion Source No. 3) has been constructed this year. The main purpose of this source is to provide highly charged ion beams for atomic physics and surface physics research. The design of this ion source is based on the IMP 14.5 GHz ECR ion source (LECR2—Lanzhou Electron Cyclotron Resonance Ion Source No. 2) with double rf heating by inserting waveguide directly and aluminum chamber. Furthermore, the volume of the plasma chamber is larger than that of LECR2 so as to increase the rf power and improve beam intensity for highly charged ions. But the hexapole field on the chamber wall is kept the same value in order to compare with the performance of LECR2. After only four days conditioning the first test results were obtained. The final result of this ion source is expected to be better than LECR2's. © 2002 American Institute of Physics.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 10
    Digitale Medien
    Digitale Medien
    [S.l.] : American Institute of Physics (AIP)
    Physics of Fluids 14 (2002), S. 61-74 
    ISSN: 1089-7666
    Quelle: AIP Digital Archive
    Thema: Physik
    Notizen: The film flow of a two-dimensional suspension of neutrally buoyant liquid drops down an inclined plane wall is investigated by numerical simulation in the limit of vanishing Reynolds numbers. The results show that well-separated and solitary drops migrate toward an equilibrium position located between the free surface and the wall, for a broad range of the fluid physical properties and flow conditions. The precise location of the equilibrium position is determined by the ratio of the drop viscosity and the viscosity of the film fluid, and by the deformability of the drop interface and free surface expressed by the drop and free-surface capillary numbers. The motion of a periodic file of drops is found to be unstable to periodic perturbations whose amplitude exceeds a critical threshold. The instability leads to periodic accumulation of drops within bands developing in the streamwise direction. Large scale numerical simulations of dilute and concentrated random systems show that the drops spontaneously migrate toward the film interior, thereby causing the development of particle-free zones along the inclined wall and free surface. In contrast to the case of pressure-driven channel flow where peaks in the drop number density distribution are observed near both walls, in the case film flow a peak develops near the inclined plane but not near the free surface. © 2002 American Institute of Physics.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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