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  • 1
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 1997-03-07
    Description: An x-ray spectroscopic study of scleractinian coral skeletons indicated that, although some strontium substitutes for calcium in the aragonite structure, at concentrations of about 7500 parts per million, as much as 40 percent of the strontium resides in strontianite (SrCO3). A doublet peak in the Fourier transform of the extended x-ray absorption fine structure of the coral corresponded to six metal and 13 oxygen neighbors surrounding strontium at about 4.05 angstroms in strontium-substituted aragonite and at about 4.21 angstroms in strontianite. Thus, the mechanism of the temperature-sensitive partitioning of strontium between seawater and coral skeleton used for paleothermometry is unexpectedly complex.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Greegor, R B -- Pingitore, N E Jr -- Lytle, F W -- New York, N.Y. -- Science. 1997 Mar 7;275(5305):1452-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Boeing Company, Seattle, WA 98124, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9072808" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Calcium/analysis ; Calcium Carbonate/*analysis ; Carbonates/*analysis ; Cnidaria/*chemistry ; Fourier Analysis ; Spectrum Analysis ; Strontium/*analysis ; Temperature
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 2
    Publication Date: 1997-05-09
    Description: Large dynamic nuclear polarization signal enhancements (up to a factor of 100) were obtained in the solid-state magic-angle spinning nuclear magnetic resonance (NMR) spectra of arginine and the protein T4 lysozyme in frozen glycerol-water solutions with the use of dynamic nuclear polarization. Polarization was transferred from the unpaired electrons of nitroxide free radicals to nuclear spins through microwave irradiation near the electron paramagnetic resonance frequency. This approach may be a generally applicable signal enhancement scheme for the high-resolution solid-state NMR spectroscopy of biomolecules.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hall, D A -- Maus, D C -- Gerfen, G J -- Inati, S J -- Becerra, L R -- Dahlquist, F W -- Griffin, R G -- GM-35382/GM/NIGMS NIH HHS/ -- RR-00995/RR/NCRR NIH HHS/ -- RR-05539/RR/NCRR NIH HHS/ -- etc. -- New York, N.Y. -- Science. 1997 May 9;276(5314):930-2.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Francis Bitter Magnet Laboratory and Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9139651" target="_blank"〉PubMed〈/a〉
    Keywords: Arginine/*chemistry ; Bacteriophage T4/enzymology ; Cyclic N-Oxides ; Electrons ; Free Radicals ; Freezing ; Magnetic Resonance Spectroscopy/*methods ; Microwaves ; Muramidase/*chemistry ; Solutions ; Spin Labels
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 3
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 1997-10-27
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lipfert, F W -- New York, N.Y. -- Science. 1997 Oct 3;278(5335):19-20.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9340746" target="_blank"〉PubMed〈/a〉
    Keywords: Air Pollution/*adverse effects ; Cohort Studies ; Confounding Factors (Epidemiology) ; Humans ; *Mortality ; United States
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 4
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 1997-09-12
    Description: In the developing nervous system, glial cells guide axons to their target areas, but it is unknown whether they help neurons to establish functional synaptic connections. The role of glial cells in synapse formation and function was studied in cultures of purified neurons from the rat central nervous system. In glia-free cultures, retinal ganglion cells formed synapses with normal ultrastructure but displayed little spontaneous synaptic activity and high failure rates in evoked synaptic transmission. In cocultures with neuroglia, the frequency and amplitude of spontaneous postsynaptic currents were potentiated by 70-fold and 5-fold, respectively, and fewer transmission failures occurred. Glial cells increased the action potential-independent quantal release by 12-fold without affecting neuronal survival. Thus, developing neurons in culture form inefficient synapses that require glial signals to become fully functional.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Pfrieger, F W -- Barres, B A -- New York, N.Y. -- Science. 1997 Sep 12;277(5332):1684-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Neurobiology, Stanford University, School of Medicine, Sherman Fairchild Science Building, 299 Campus Drive, Stanford, CA 94305-5125, USA. fpfrieg@mdc-berlin.de〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9287225" target="_blank"〉PubMed〈/a〉
    Keywords: Action Potentials ; Animals ; Astrocytes/physiology ; Cell Survival ; Cells, Cultured ; Coculture Techniques ; Microglia/physiology ; Neuroglia/*physiology ; Oligodendroglia/physiology ; Rats ; Rats, Sprague-Dawley ; Retinal Ganglion Cells/cytology/*physiology/ultrastructure ; Synapses/*physiology/ultrastructure ; *Synaptic Transmission
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 5
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 1997-10-27
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉DeGrado, W F -- New York, N.Y. -- Science. 1997 Oct 3;278(5335):80-1.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6059, USA. wdegrado@mail.med.upenn.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9340760" target="_blank"〉PubMed〈/a〉
    Keywords: *Algorithms ; Amino Acid Sequence ; Computer Simulation ; DNA-Binding Proteins/chemical synthesis/*chemistry ; Models, Molecular ; *Protein Conformation ; *Protein Engineering ; Protein Folding ; Protein Structure, Tertiary ; Software ; Thermodynamics ; Transcription Factors/chemical synthesis/*chemistry ; Zinc Fingers
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 6
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 1997-02-28
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Dempster, W F -- New York, N.Y. -- Science. 1997 Feb 28;275(5304):1247-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9064774" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Arizona ; Atmosphere ; *Ecological Systems, Closed ; *Ecosystem ; Humans
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 7
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 1997-06-27
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Benaron, D A -- Cheong, W F -- Stevenson, D K -- New York, N.Y. -- Science. 1997 Jun 27;276(5321):2002-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Biomedical Optics Group at the Medical Free Electron Laser Program, Hansen Experimental Physics Laboratory (HEPL), Stanford University, Stanford, CA 94034, USA. benaron@forsythe.stanford.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9221510" target="_blank"〉PubMed〈/a〉
    Keywords: Catheterization/instrumentation ; Esophagus/anatomy & histology ; Fiber Optic Technology ; Humans ; Interferometry/instrumentation ; Light ; Photons ; Scattering, Radiation ; Tomography/instrumentation/*methods
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 8
    Publication Date: 1997-07-25
    Description: Chromosome movements and spindle dynamics were visualized in living cells of the budding yeast Saccharomyces cerevisiae. Individual chromosomal loci were detected by expression of a protein fusion between green fluorescent protein (GFP) and the Lac repressor, which bound to an array of Lac operator binding sites integrated into the chromosome. Spindle microtubules were detected by expression of a protein fusion between GFP and Tub1, the major alpha tubulin. Spindle elongation and chromosome separation exhibited biphasic kinetics, and centromeres separated before telomeres. Budding yeast did not exhibit a conventional metaphase chromosome alignment but did show anaphase A, movement of the chromosomes to the poles.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Straight, A F -- Marshall, W F -- Sedat, J W -- Murray, A W -- New York, N.Y. -- Science. 1997 Jul 25;277(5325):574-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Physiology, Box 0444, School of Medicine, University of California at San Francisco, San Francisco, CA 94143-0444, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9228009" target="_blank"〉PubMed〈/a〉
    Keywords: *Anaphase ; Bacterial Proteins/metabolism ; Centromere/chemistry/physiology ; Chromatids/physiology ; Chromosomes, Fungal/chemistry/*physiology ; *Escherichia coli Proteins ; Green Fluorescent Proteins ; Lac Repressors ; Luminescent Proteins ; *Metaphase ; Microscopy, Fluorescence ; Microtubules/ultrastructure ; *Mitosis ; Movement ; Operator Regions, Genetic ; Recombinant Fusion Proteins ; Repressor Proteins/metabolism ; Saccharomyces cerevisiae/*cytology ; Spindle Apparatus/physiology/ultrastructure ; Telomere/physiology ; Tubulin/analysis
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 9
    Publication Date: 1997-07-25
    Description: C-peptide, a cleavage product from the processing of proinsulin to insulin, has been considered to possess little if any biological activity other than its participation in insulin synthesis. Injection of human C-peptide prevented or attenuated vascular and neural (electrophysiological) dysfunction and impaired Na+- and K+-dependent adenosine triphosphate activity in tissues of diabetic rats. Nonpolar amino acids in the midportion of the peptide were required for these biological effects. Synthetic reverse sequence (retro) and all-D-amino acid (enantio) C-peptides were equipotent to native C-peptide, which indicates that the effects of C-peptide on diabetic vascular and neural dysfunction were mediated by nonchiral interactions instead of stereospecific receptors or binding sites.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ido, Y -- Vindigni, A -- Chang, K -- Stramm, L -- Chance, R -- Heath, W F -- DiMarchi, R D -- Di Cera, E -- Williamson, J R -- EY 06600/EY/NEI NIH HHS/ -- HL 39934/HL/NHLBI NIH HHS/ -- HL 58141/HL/NHLBI NIH HHS/ -- etc. -- New York, N.Y. -- Science. 1997 Jul 25;277(5325):563-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pathology, Washington University School of Medicine, 660 South Euclid Avenue, St. Louis, MO 63110, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9228006" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Sequence ; Animals ; Blood Circulation/drug effects ; Blood Glucose/metabolism ; C-Peptide/*chemistry/pharmacology/*therapeutic use ; Capillary Permeability/drug effects ; Circular Dichroism ; Diabetes Mellitus, Experimental/drug therapy/physiopathology ; Diabetic Angiopathies/*prevention & control ; Diabetic Neuropathies/*prevention & control ; Humans ; Male ; Molecular Sequence Data ; Neural Conduction/drug effects ; Peptide Fragments/pharmacology ; Protein Structure, Secondary ; Rats ; Rats, Sprague-Dawley ; Sodium-Potassium-Exchanging ATPase/metabolism ; Stereoisomerism
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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