ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Springer  (33)
  • Blackwell Science Ltd.
  • Geological Society of America (GSA)
  • 2015-2019  (4)
  • 2000-2004
  • 1995-1999  (31)
  • 1950-1954
  • 2016  (4)
  • 1998  (17)
  • 1997  (14)
  • 1
    Electronic Resource
    Electronic Resource
    Microfluorometric analyses of glycogen in freshly dissected, single skeletal muscle fibres of the cane toad using a mechanically skinned fibre preparation (1998)
    Springer
    Journal of muscle research and cell motility 19 (1998), S. 631-638 
    Details
    ISSN: 1573-2657
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The main objective of this study was to analyse glycogen in single muscle fibres, using a recently developed microfluorometric method which detects subpicomol amounts of NADPH, glucose and glycogen (as glucosyl units) (detection limit 0.16–0.17pmol in a 25nl sample) without fluorochrome amplification. The fibres were freshly dissected from the twitch region of the iliofibularis muscle of the cane toad (Bufo marinus), and were mechanically skinned under paraffin oil to gain access to the intracellular compartments. The results show that: (1) glycogen concentrations in toad skeletal muscle fibres range between 25.8 and 369mmol glucosyl units/litre fibre volume; (2) there is a large variation in glycogen content between individual fibres from the iliofibularis muscle of one animal; (3) there are seasonal differences in the glycogen content of toad single muscle fibres; (4) the total amount of glycogen in single muscle fibres of the toad does not decrease significantly when storing the tissue, under paraffin oil, at 20–25°C for up to 6h or at 4°C for up to 24h; and (5) 15–26% of fibre glycogen can be washed in an aqueous solution at pH 5–7, within 5min, while 74–85% of fibre glycogen remains associated with the washed skinned fibre, even after 40min exposure of the skinned fibre preparation to the aqueous environment. The retention of most glycogen in the fibre preparation after mechanical removal of the plasma membrane and extensive washing indicates that in toad skeletal muscle fibres the largest proportion of glycogen is tightly bound to intracellular structures. The results also show that the skinned muscle fibre preparation is well suited for microfluorometric glycogen determination, since low molecular weight non-glycogen contributors to the fluorescence signal can be removed from the myoplasmic space prior to the glycogen hydrolysis step.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005377030193
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Metamorphic evolution of calcsilicate granulites near Battye Glacier, northern Prince Charles Mountains, East Antarctica (1997)
    Oxford, UK : Blackwell Science Ltd.
    Journal of metamorphic geology 15 (1997), S. 0 
    Details
    ISSN: 1525-1314
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences
    Notes: Calcsilicate granulites of probable Middle Proterozoic age (c.1000–1100 Ma) in the vicinity of Battye Glacier, northern Prince Charles Mountains, East Antarctica, contain prograde metamorphic assemblages comprising various combinations of wollastonite, scapolite, clinopyroxene, An-rich plagioclase, calcite, quartz, titanite and, rarely, orthoclase, ilmenite, phlogopite and graphite. Comparison of the prograde assemblages with calculated and experimentally determined phase relations in the simple CaO–Al2O3–SiO2–CO2–H2O system suggests peak metamorphism at ≥835 °C in the presence (in wollastonite-bearing assemblages at least) of a CO2-bearing fluid (XCO≥0.3) at a probable pressure of 6–7 kbar.Well-preserved retrograde reaction textures represent: (1) breakdown of scapolite to anorthite+calcite±quartz; (2) formation of grossular–andradite garnet and, locally, (3) epidote, both principally by reactions involving scapolite breakdown products and clinopyroxene; (4) local coupled replacement of clinopyroxene and ilmenite by hornblende and titanite, respectively; and finally (5) local sericitization of prograde and retrograde plagioclase. These retrograde reactions are interpreted to be the result of cooling and variable infiltration by H2O-rich fluids, possibly derived from crystallizing pegmatitic intrusions and segregations that may be partial melts, which are common throughout the area.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1525-1314.1997.00024.x
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    In-situ scanning tunnelling microscopy of hydrogenated amorphous silicon and microcrystalline silicon (1998)
    Springer
    Applied physics 66 (1998), S. 1101-1105 
    Details
    ISSN: 1432-0630
    Keywords: PACS: 68.55; 72.20; 81.10
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003390051305
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    A comparison of global tropospheric teleconnections using observed satellite and general circulation model total ozone column data for 1979–91 (1998)
    Springer
    Climate dynamics 14 (1998), S. 133-150 
    Details
    ISSN: 1432-0894
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract  The total ozone column is well correlated with tropospheric fields such as the heights of the upper tropospheric geopotential surfaces and thus it can provide useful information on temporal variability in the troposphere. The global availability of long period satellite measurements of the total ozone column, taken by the TOMS instruments since 1978, provides a valuable and independent data set for use in studies of seasonal and interannual climate variability. In this study, the global low-frequency seasonal teleconnections in the observed TOMS data from 1979–91 have been investigated using seasonal teleconnectivity maps and empirical orthogonal function analysis. They have also been compared with the results from a simulation made with the atmospheric GCM at Météo-France, having prescribed observed sea surface temperatures for the same period. In the observed total ozone, strong ENSO-related wave number one longitudinal dipole patterns are seen in both the tropics and in the Southern Hemisphere extratropics. The model shows much weaker variability in total ozone yet appears to be able to capture similar teleconnection patterns in the tropics related to ENSO. In the SH extratropics, the model total ozone shows a strong wave number 3 response rather than the wave number one dipole seen in the observations. A wave number 3 response is also evident in the 200 hPa geopotential height simulated by the model and in the NCEP analysis, and is consistent with the response in a linearised barotropic model forced in the Indonesian region. The different responses in the modelled and observed total ozone, suggest that tropopause effect is not the major factor in the SH extratropics, and it is likely that horizontal ozone transport also plays a role in this region. Despite a generally poor simulation of the zonal mean total ozone, the model was able to capture the anomalous strengthening of the SH stationary waves during austral spring of 1988, related to an intense stratosphere sudden warming.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003820050214
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Effects of pollination intensity on the vigor of the sporophytic and gametophytic generation of Cucurbita texana (1997)
    Springer
    Sexual plant reproduction 10 (1997), S. 236-240 
    Details
    ISSN: 1432-2145
    Keywords: Key words Cucurbita texana ; Pollen ; Pollen performance ; Pollen competition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We examined the effects of pollen competition (pollen load size) on sporophytic vigor and gametophytic performance in Cucurbita texana, a wild gourd, while controlling for alternative interpretations of the data. Under field conditions we compared the vigor of progeny produced from large and small pollen loads and examined the in vitro performance of the pollen produced by the progeny. We found that the progeny from large pollen loads germinated faster and had a greater reproductive output (male flowers and fruits) than progeny produced from small pollen loads. In addition, we found that the pollen produced on plants derived from large pollen loads grew faster in vitro than the pollen produced on plants derived from small pollen loads. These findings indicate that pollen competition affects the performance of the resulting sporophytic generation and the microgametophytes they produce.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050092
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Variation in sporophytic and gametophytic vigor in wild and cultivated varieties of Cucurbita pepo and their F1 and F2 generations (1998)
    Springer
    Sexual plant reproduction 11 (1998), S. 265-271 
    Details
    ISSN: 1432-2145
    Keywords: Key words Cucurbita ; Pollen tube growth ; Pollen performance ; Hybridization ; Variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  This study examines sporophytic and gametophytic vigor in wild and cultivated varieties of Cucurbita pepo L. and their hybrids in order to determine whether hybrid vigor extends to the microgametophyte generation. It also examines the variation in sporophytic and gametophytic vigor to discern the non-genetic influences of pollen provisioning by the sporophyte on pollen performance from the genetic influences of the microgametophyte’s own genotype on pollen performance. A cultivated and a wild C. pepo and their F1 and the F2 generations were grown under field conditions and flower and fruit production were monitored over one summer. We found that the four types of plants differed significantly in the number of male and female flowers and the number of fruits they produced. The F1 plants produced significantly more male flowers and marginally more female flowers and fruits than the parental lines. To estimate gametophytic vigor pollen was germinated in vitro and pollen tube length measured after 30 min. We found that pollen tubes from the F1 plants had significantly greater growth than tubes from the parental lines or the F2 generation, indicating that hybrid vigor extends to the microgametophytic generation. By partitioning the variance of pollen tube growth into ’within’ and ’among’ plant components of variation, we were able to show that the genotype of the microgametophyte influences pollen performance in vitro, but that expression of hybrid vigor in the microgametophyte is likely to be due to an environmental effect related to provisioning of the pollen grains during development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050152
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Cloning and characterisation of glutamine synthetase from Colletotrichum gloeosporioides and demonstration of elevated expression during pathogenesis on Stylosanthes guianensis (1997)
    Springer
    Current genetics 31 (1997), S. 447-454 
    Details
    ISSN: 1432-0983
    Keywords: Key words Expression quantification ; Glutamine synthetase ; Pathogenesis ; Nitrogen metabolism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Experiments were designed to clone and identify genes of the fungal phytopathogen Colletotrichum gloeosporioides expressed at high levels during growth on the compatible host Stylosanthes guianensis when compared with expression in axenic culture. A cDNA clone (pCgGS) that hybridised preferentially to a cDNA probe prepared from infected leaves was isolated by the differential screening of a cDNA library from a nitrogen-starved axenic culture of C. gloeosporioides. The DNA sequence of pCgGS is highly homologous to genes for glutamine synthetase (GS) in other organisms. pCgGS contained all of the conserved regions assigned as catalytic domains in GS enzymes. Comparison with genomic sequences indicated that in C. gloeosporioides the GS gene is present as a single copy with three introns. To our knowledge this is the first report of the cloning of a GS from a filamentous fungus. A second clone (pCgRL1) was also isolated and represented a partial cDNA of the 25s rRNA of C. gloeosporioides. Because pCgRL1 did not hybridise to plant rRNA under high-stringency hybridisation conditions, it was used as a reference to quantify the expression of fungal GS mRNA during pathogenesis in S. guianensis compared to fungal growth in axenic culture. The results indicated that elevated expression of GS occurred during pathogenesis of C. gloeosporioides on S. guianensis, particularly at early stages of infection where expression was about six-times higher than during growth in rich culture media. This work also demonstrates that fungal-specific 25s rRNA fragments, such as pCgRL1, have considerable utility as a reference for quantifying pathogen gene expression in infected plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050228
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Hydrogen plasma transport and deposition of films from a solid boron source (1997)
    Springer
    Journal of materials science 32 (1997), S. 3277-3282 
    Details
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Thin boron films were produced on Si substrates from a solid boron source and a hydrogen plasma. The plasma was generated using a 13.56 MHz generator and films were deposited with a forward radio frequency (RF) power of 2.0 kW. At pressures from 0.931–2.26×102 Pa under high hydrogen concentrations a capacitively coupled plasma (CCP) was observed whereas at low hydrogen concentrations an inductively coupled plasma (ICP) was observed. The films were predominantly deposited with an ICP but in one case a film was deposited using a CCP discharge. The deposited films consisted primarily of boron, but they also contained oxygen and silicon. The films were amorphous at 225 and 350°C, but revealed X-ray diffractions at 475°C. It was concluded that the hydrogen concentration, RF plasma power and surface temperature as well as the plasma-boron source interactions strongly influenced the film thickness and composition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018631608153
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Isolation and characterisation of microsatellites from hexaploid bread wheat (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 557-563 
    Details
    ISSN: 1432-2242
    Keywords: Key words Wheat ; PCR ; Microsatellite ; Simple sequence repeats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The development of large panels of simple-to-analyse genetic markers for tagging agronomically important genes and diversity studies in hexaploid bread wheat is an important goal in applied cereal genetic research. We have isolated and sequenced over 200 clones containing microsatellites from the wheat genome and have tested 153 primer pairs for genetic polymorphism using a panel of ten wheat varieties, including the parents of our main mapping cross. A subset comprising 49 primer pairs detects 76 loci, of which 74 can be unequivocably allocated to one of the wheat chromosomes. A relatively low frequency of the loci detected are from the D genome, and these loci show less polymorphism than those from the A and B genomes. Generally, the microsatellites show high levels of genetic polymorphism and an average of 3.5 alleles per locus with an average polymorphism information content (PIC), value of 0.51. The observed levels of polymorphism are positively correlated with the length of the microsatellite repeats. A high proportion, approximately two-thirds, of primer pairs designed to detect simple sequence repeat (SSR) variation in wheat do not generate the expected amplification products and, more significantly, often generate unresolvable PCR products. In general, our results agree closely with those obtained from other recent studies using microsatellites in plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050451
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Fifty new microsatellite loci for the wheat genetic map (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 946-949 
    Details
    ISSN: 1432-2242
    Keywords: Key words Wheat ; Microsatellite markers ; Genetic map
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Hexaploid bread wheat (Triticum aestivum) has low levels of RFLP. Simple sequence repeats, however, show high levels of polymorphism and are therefore especially useful in intervarietal breeding applications. We present 53 newly mapped microsatellite loci for the wheat genetic map, 41 primary loci and 12 additional loci from these same primer pairs. Markers have been accredited with a quality score on a scale of 1–5 which describes the complexity of the amplification product profile from each primer pair.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050975
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...