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  • Springer  (7)
  • Blackwell Publishing Ltd  (1)
  • Oxford University Press
  • Wiley-Blackwell
  • 2015-2019
  • 2005-2009
  • 1990-1994  (8)
  • 1940-1944
  • 1993  (8)
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  • 2015-2019
  • 2005-2009
  • 1990-1994  (8)
  • 1940-1944
Year
  • 1
    Electronic Resource
    Electronic Resource
    ATP-dependent carbon transport in perfused Chara cells (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 16 (1993), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Carbon transport across the plasma membrane, and carbon fixation were measured in perfused Chara internodal cells. These parameters were measured in external media of pH 5·5 and pH 8·5, where CO2 and HCO3- are, respectively, the predominant carbon species in both light and dark conditions. Cells perfused with medium containing ATP could utilize both CO2 and HCO3- from the external medium in the light. Photosynthetic carbon fixation activity was always higher at pH 5·5 than at pH 8·5. When cells were perfused either with medium containing hexokinase and 2-deoxyglucose to deplete ATP from the cytosol (HK medium) or with medium containing vanadate, a specific inhibitor of the plasma membrane H+-ATPase (V medium), photosynthetic carbon fixation was strongly inhibited at both pH 5·5 and 8·5. Perfusion of cells with medium containing pyruvate kinase and phosphoenolpyruvate (PEP) to maximally activate the H+-ATPase (PK medium), stimulated the photosynthetic carbon fixation activities. Oxygen evolution of isolated chloroplasts and the carbon fixation of cells supplied 14C intracellularly were not inhibited by perfusion media containing either hexokinase and 2-deoxyglucose or vanadate. The results indicate that Chara cells possess CO2 and HCO3- transport systems energized by ATP and sensitive to vanadate in the light. In the dark, intact cells also fix carbon. By contrast, in cells perfused with medium containing ATP, no carbon fixation was detected in 1 mol m -3 total dissolved inorganic carbon (TDIC) at pH 8·5. By increasing TDIC to 10 mol m-3, dark fixation became detectable, although it was still lower than that of intact cells at 1mol m-3 TDIC. Addition of PEP or PEP and PEP carboxylase to the perfusion media significantly increased the dark-carbon fixation. Perfusion with vanadate had no effect on the dark-carbon fixation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3040.1993.tb00483.x
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  • 2
    Electronic Resource
    Electronic Resource
    New fluorescent labels for time-resolved detection of biomolecules (1993)
    Springer
    Journal of fluorescence 3 (1993), S. 131-139 
    Details
    ISSN: 1573-4994
    Keywords: Fluorescence ; multiplex dyes ; photochemistry ; time-resolved spectroscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract New dyes with characteristic fluorescence lifetimes have been developed for bioanalytical applications. Based upon the concept of “multiplex dyes,” we have designed rhodamine dyes with nearly identical absorption and emission spectral characteristics but different fluorescence lifetimes. Extending this principle to applications with laser diodes, new rhodamines with functional groups for covalent coupling of analytes have been developed. The new labels exhibit absortion and fluorescence beyond 600 nm and have a high quantum efficiency, even in aqueous buffer systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00862730
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  • 3
    Electronic Resource
    Electronic Resource
    Genome analysis of intergeneric hybrids of apomictic and sexual Australian Elymus species with wheat, barley and rye: implication for the transfer of apomixis to cereals (1993)
    Springer
    Theoretical and applied genetics 86 (1993), S. 288-294 
    Details
    ISSN: 1432-2242
    Keywords: Apomixis ; Wheat ; Barley ; Rye ; Wide hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Four hybrids were obtained between three Australian Elymus taxa and three cereal grains: wheat, rye, and barley. Mean meiotic metaphase-I configurations were 41.14 I, 0.42 rod II, 0.003 ring II, and 0.01 III for E. scabrus var ‘plurinervis’ x Triticum aestivum (1 hybrid plant), 22.27 I, 2.63 rod II, 0.06 ring II, and 0.12 III for E. scabrus var ‘scabrus’ x Secale cereale (4 hybrid plants), and 26.65 I, 0.66 rod II, 0.00 ring II, and 0.01 III for E. scabrus var ‘plurinervis’ x Hordeum vulgare (13 hybrid plants). The I genome of barley also paired very little in a BIII hybrid of apomictic E. rectisetus x H. vulgare (2 hybrid plants). Megasporogenesis in this BIII hybrid was at least facultatively apomeiotic, with the same sort of nuclear elongation, apomeiotic division, and dyad formation seen previously in E. rectisetus itself. All four hybrid combinations were sterile. While spike morphology in the E. scabrus x T. aestivum and E. scabrus x H. vulgare hybrids were intermediate to their parents, E. scabrus x S. cereale and E. rectisetus x H. vulgare looked like their maternal parents.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222090
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  • 4
    Electronic Resource
    Electronic Resource
    Acrylic acid copolymer nanoparticles for drug delivery. Part II: Characterization of nanoparticles surface-modified by adsorption of ethoxylated surfactants (1993)
    Springer
    Colloid & polymer science 271 (1993), S. 100-105 
    Details
    ISSN: 1435-1536
    Keywords: Nanoparticles ; acrylic acid copolymer ; ethoxylated surfactants ; hydrophobic interaction chromatography (HIC) ; poloxamer ; poloxamine ; antarox
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Copolymer nanoparticles of acrylic acid, acrylic amide, acrylic butylester, and methacrylic methylester with increasing content of acrylic acid were produced and surface-modified by adsorption of nonionic (Poloxamer 407, Poloxamine 908, Antarox CO 990) and ionic (Gafac RE 960) surfactants. The coated particles were characterized with regard to parameters relevant for the in vivo organ distribution: coating layer thickness, charge-reducing effect of the coating layer and surface hydrophobicity. Gafac was found to form highly charged surface layers leading to recognition by the reticuloendothelial system (RES). The hydrophobicity of the coating layers decreased with increasing thickness. The thickest coating layers were found on the most hydrophobic particles possessing least content of acrylic acid (1.9%). These particles coated with the nonionics were regarded as sufficiently hydrophilic to potentially reduce the uptake by the RES in vivo. The properties of coating layers can therefore be optimized by variation of the monomer ratios in copolymers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00652310
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  • 5
    Electronic Resource
    Electronic Resource
    Excitation and decay processes in helium clusters studied by fluorescence spectroscopy (1993)
    Springer
    The European physical journal 26 (1993), S. 175-177 
    Details
    ISSN: 1434-6079
    Keywords: 36.40 ; 67.90+Z ; 71.90+Q
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Excitation and decay processes of helium clusters are investigated with fluorescence methods. The results differ remarkably from that obtained for the heavier rare gas clusters. They are discussed in view of the unusual structural and electronic properties of helium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01429135
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  • 6
    Electronic Resource
    Electronic Resource
    Excitation and decay processes in helium clusters studied by fluorescence spectroscopy (1993)
    Springer
    The European physical journal 26 (1993), S. 382-384 
    Details
    ISSN: 1434-6079
    Keywords: 36.40 ; 67.90+Z ; 71.90+Q
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Excitation and decay processes of helium clusters are investigated with fluorescence methods. The results differ remarkably from that obtained for the heavier rare gas clusters. They are discussed in view of the unusual structural and electronic properties of helium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01429201
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  • 7
    Electronic Resource
    Electronic Resource
    Excitation and radiative decay of neutral and ionic ArN clusters studied by fluorescence spectroscopy (1993)
    Springer
    The European physical journal 26 (1993), S. 370-372 
    Details
    ISSN: 1434-6079
    Keywords: 36.40 ; 78.55-M
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Excitation and decay processes of neutral and ionized ArN clusters are analysed using fluorescence spectroscopy with synchrotron radiation. The fluorescence yield of ionized ArN clusters is resonantly enhanced after excitation from states related to the atomic 3s levels.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01429198
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  • 8
    Electronic Resource
    Electronic Resource
    Pyrolysis-mass spectrometry of whole soils, soil particle-size fractions, litter materials and humic substances: statistical evaluation of sample weight, residue, volatilized matter and total ion intensity (1993)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 346 (1993), S. 697-703 
    Details
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A set of 115 samples with known organic carbon (Corg) concentrations, divided in the sample groups whole soils (n=39), soil particle-size fractions (n=33) and litter materials and humic substances (n=43), was investigated repeatedly (3–6 replicates) by pyrolysis-mass spectrometry using field ionization as a soft ionization mode (Py-FIMS). Statistical evaluation of the results supplied basic information on the reproducibility of Py-FIMS measurements of these complex organic materials, as well as on the influence of Corg concentration on volatilization and total ion intensity (TII). The relative error in measurement for sample weight amounted to 10%, for percentage of residue to 2%, for percentage of volatilized matter to 5% and both for TII/mg sample and TII/mg Corg to 17%. The relative errors in measurement of sample weight, TII/mg sample and TII/mg Corg of the total sample set corresponded with those of each group, however differences were observed for the percentages of residue and volatilized matter. Linear correlation and regression analyses show that larger Corg concentrations result in a significant increase in percentage of volatilized matter and TII/mg sample, whereas percentages of residue were significantly decreased. On the average of all samples, 75.2% of the variation of TII/mg sample was due to Corg concentration and the percentage of volatilized matter. For the total sample set covering a wide range of plant and humus materials, the relative error in measurement of TII/mg sample was independent of Corg concentration, sample weight, percentage of residue and percentage of volatilized matter.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00321275
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