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  • Artikel  (16)
  • Male  (16)
  • Cell & Developmental Biology
  • American Association for the Advancement of Science (AAAS)  (16)
  • 2005-2009
  • 1990-1994  (16)
  • 1992  (16)
  • Chemie und Pharmazie  (16)
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  • Artikel  (16)
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  • American Association for the Advancement of Science (AAAS)  (16)
  • Wiley-Blackwell  (27)
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  • 2005-2009
  • 1990-1994  (16)
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  • 1
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    Structure and functional expression of an omega-conotoxin-sensitive human N-type calcium channel (1992)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1992-07-17
    Beschreibung: N-type calcium channels are omega-conotoxin (omega-CgTx)-sensitive, voltage-dependent ion channels involved in the control of neurotransmitter release from neurons. Multiple subtypes of voltage-dependent calcium channel complexes exist, and it is the alpha 1 subunit of the complex that forms the pore through which calcium enters the cell. The primary structures of human neuronal calcium channel alpha 1B subunits were deduced by the characterization of overlapping complementary DNAs. Two forms (alpha 1B-1 and alpha 1B-2) were identified in human neuroblastoma (IMR32) cells and in the central nervous system, but not in skeletal muscle or aorta tissues. The alpha 1B-1 subunit directs the recombinant expression of N-type calcium channel activity when it is transiently co-expressed with human neuronal beta 2 and alpha 2b subunits in mammalian HEK293 cells. The recombinant channel was irreversibly blocked by omega-CgTx but was insensitive to dihydropyridines. The alpha 1B-1 alpha 2b beta 2-transfected cells displayed a single class of saturable, high-affinity (dissociation constant = 55 pM) omega-CgTx binding sites. Co-expression of the beta 2 subunit was necessary for N-type channel activity, whereas the alpha 2b subunit appeared to modulate the expression of the channel. The heterogeneity of alpha 1B subunits, along with the heterogeneity of alpha 2 and beta subunits, is consistent with multiple, biophysically distinct N-type calcium channels.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Williams, M E -- Brust, P F -- Feldman, D H -- Patthi, S -- Simerson, S -- Maroufi, A -- McCue, A F -- Velicelebi, G -- Ellis, S B -- Harpold, M M -- New York, N.Y. -- Science. 1992 Jul 17;257(5068):389-95.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉SIBIA, Inc., La Jolla, CA 92037.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1321501" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Amino Acid Sequence ; Calcium/metabolism ; Calcium Channels/*drug effects/*genetics/*metabolism ; Cell Line ; Female ; Humans ; Male ; Membrane Potentials ; Molecular Sequence Data ; Neuroblastoma/metabolism ; Peptides, Cyclic/*pharmacology ; Sequence Alignment ; Sequence Homology, Nucleic Acid ; Transfection ; omega-Conotoxin GVIA
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
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    Nitric oxide: a physiologic mediator of penile erection (1992)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1992-07-17
    Beschreibung: Nitric oxide (NO) is a cytotoxic agent of macrophages, a messenger molecule of neurons, and a vasodilator produced by endothelial cells. NO synthase, the synthetic enzyme for NO, was localized to rat penile neurons innervating the corpora cavernosa and to neuronal plexuses in the adventitial layer of penile arteries. Small doses of NO synthase inhibitors abolished electrophysiologically induced penile erections. These results establish NO as a physiologic mediator of erectile function.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Burnett, A L -- Lowenstein, C J -- Bredt, D S -- Chang, T S -- Snyder, S H -- DA-00266/DA/NIDA NIH HHS/ -- DK-19300/DK/NIDDK NIH HHS/ -- MH-18501/MH/NIMH NIH HHS/ -- etc. -- New York, N.Y. -- Science. 1992 Jul 17;257(5068):401-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Urology, Johns Hopkins University School of Medicine, Baltimore, MD 21205.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1378650" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Amino Acid Oxidoreductases/antagonists & inhibitors/biosynthesis ; Animals ; Arginine/analogs & derivatives/pharmacology ; Dose-Response Relationship, Drug ; Male ; Nerve Fibers/metabolism ; *Nitric Oxide ; Nitric Oxide Synthase ; Nitroarginine ; Penile Erection/drug effects/*physiology ; Penis/metabolism ; Rats ; Urethra/metabolism
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 3
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    Block of Ca2+ wave and Ca2+ oscillation by antibody to the inositol 1,4,5-trisphosphate receptor in fertilized hamster eggs (1992)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1992-07-10
    Beschreibung: The concentration of cytoplasmic free calcium (Ca2+) increases in various stimulated cells in a wave (Ca2+ wave) and in periodic transients (Ca2+ oscillations). These phenomena are explained by inositol 1,4,5-trisphosphate (IP3)-induced Ca2+ release (IICR) and Ca(2+)-induced Ca2+ release (CICR) from separate intracellular stores, but decisive evidence is lacking. A monoclonal antibody to the IP3 receptor inhibited both IICR and CICR upon injection of IP3 and Ca2+ into hamster eggs, respectively. The antibody completely blocked sperm-induced Ca2+ waves and Ca2+ oscillations. The results indicate that Ca2+ release in fertilized hamster eggs is mediated solely by the IP3 receptor, and Ca(2+)-sensitized IICR, but not CICR, generates Ca2+ waves and Ca2+ oscillations.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Miyazaki, S -- Yuzaki, M -- Nakada, K -- Shirakawa, H -- Nakanishi, S -- Nakade, S -- Mikoshiba, K -- New York, N.Y. -- Science. 1992 Jul 10;257(5067):251-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Physiology, Tokyo Women's Medical College, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1321497" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Antibodies, Monoclonal ; Caffeine/pharmacology ; Calcium/*metabolism ; *Calcium Channels ; Cricetinae ; Dose-Response Relationship, Drug ; Fertilization/*physiology ; Immunoblotting ; Inositol 1,4,5-Trisphosphate Receptors ; Male ; Ovum/*metabolism ; Receptors, Cell Surface/drug effects/*physiology ; *Receptors, Cytoplasmic and Nuclear ; Ryanodine/pharmacology ; Spermatozoa/physiology ; Time Factors
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 4
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    Lymphoid development in mice congenitally lacking T cell receptor alpha beta-expressing cells (1992)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1992-06-05
    Beschreibung: Vertebrate T cells express either an alpha beta or gamma delta T cell receptor (TCR). The developmental relatedness of the two cell types is unresolved. alpha beta + T cells respond to specific pathogens by collaborating with immunoglobulin-producing B cells in distinct lymphoid organs such as the spleen and Peyer's patches. The precise influence of alpha beta + T cells on B cell development is poorly understood. To investigate the developmental effects of alpha beta + T cells on B cells and gamma delta + T cells, mice homozygous for a disrupted TCR alpha gene were generated. The homozygotes showed elimination of alpha beta + T cells and the loss of thymic medullae. Despite this, gamma delta + T cells developed in normal numbers, and there was an increase in splenic B cells.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Philpott, K L -- Viney, J L -- Kay, G -- Rastan, S -- Gardiner, E M -- Chae, S -- Hayday, A C -- Owen, M J -- GM37759/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1992 Jun 5;256(5062):1448-52.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Imperial Cancer Research Fund, London, United Kingdom.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1604321" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; B-Lymphocytes/*immunology ; Blastocyst ; Blotting, Southern ; Chimera ; Clone Cells ; DNA/genetics/isolation & purification ; Female ; Lymphoid Tissue/growth & development/*immunology ; Macromolecular Substances ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Mutant Strains ; Peyer's Patches/immunology ; Polymerase Chain Reaction ; Receptors, Antigen, T-Cell/*genetics ; Spleen/immunology ; T-Lymphocytes/*immunology ; Thymus Gland/immunology
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 5
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    The human Y chromosome: a 43-interval map based on naturally occurring deletions (1992)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1992-10-02
    Beschreibung: A deletion map of the human Y chromosome was constructed by testing 96 individuals with partial Y chromosomes for the presence or absence of many DNA loci. The individuals studied included XX males, XY females, and persons in whom chromosome banding had revealed translocated, deleted, isodicentric, or ring Y chromosomes. Most of the 132 Y chromosomal loci mapped were sequence-tagged sites, detected by means of the polymerase chain reaction. These studies resolved the euchromatic region (short arm, centromere, and proximal long arm) of the Y chromosome into 43 ordered intervals, all defined by naturally occurring chromosomal breakpoints and averaging less than 800 kilobases in length. This deletion map should be useful in identifying Y chromosomal genes, in exploring the origin of chromosomal disorders, and in tracing the evolution of the Y chromosome.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Vollrath, D -- Foote, S -- Hilton, A -- Brown, L G -- Beer-Romero, P -- Bogan, J S -- Page, D C -- New York, N.Y. -- Science. 1992 Oct 2;258(5079):52-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Howard Hughes Research Laboratories, Whitehead Institute, Cambridge, MA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1439769" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Base Sequence ; *Chromosome Mapping ; Electrophoresis, Polyacrylamide Gel ; Female ; *Gene Deletion ; *Genome, Human ; Humans ; Male ; Molecular Sequence Data ; Polymerase Chain Reaction ; Sequence Tagged Sites ; *Y Chromosome
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 6
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    Identification of ras oncogene mutations in the stool of patients with curable colorectal tumors (1992)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1992-04-03
    Beschreibung: Colorectal (CR) tumors are usually curable if detected before metastasis. Because genetic alterations are associated with the development of these tumors, mutant genes may be found in the stool of individuals with CR neoplasms. The stools of nine patients whose tumors contained mutations of K-ras were analyzed. In eight of the nine cases, the ras mutations were detectable in DNA purified from the stool. These patients included those with benign and malignant neoplasms from proximal and distal colonic epithelium. Thus, colorectal tumors can be detected by a noninvasive method based on the molecular pathogenesis of the disease.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sidransky, D -- Tokino, T -- Hamilton, S R -- Kinzler, K W -- Levin, B -- Frost, P -- Vogelstein, B -- CA06973/CA/NCI NIH HHS/ -- CA35494/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1992 Apr 3;256(5053):102-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Oncology, Johns Hopkins University, Baltimore, MD 21231.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1566048" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Adult ; Aged ; Amino Acid Sequence ; Base Sequence ; Blotting, Southern ; Carcinoma/diagnosis/*genetics/pathology ; Colonic Neoplasms/diagnosis/*genetics/pathology ; DNA, Neoplasm/genetics/*isolation & purification ; Feces/chemistry ; Female ; *Genes, ras ; Humans ; Male ; Middle Aged ; Molecular Sequence Data ; *Mutation ; Oligodeoxyribonucleotides ; Polymerase Chain Reaction ; Prognosis ; Rectal Neoplasms/diagnosis/*genetics/pathology
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 7
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    Myoblast therapy (1992)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1992-08-07
    Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Epstein, H F -- Fischman, D A -- Bader, D -- Changeux, J P -- Buckhold, K -- Ordahl, C P -- Hoffman, E -- Kedes, L H -- Konieczny, S -- Leinwand, L A -- New York, N.Y. -- Science. 1992 Aug 7;257(5071):738.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1496388" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Adult ; Child ; Humans ; Male ; Muscles/*transplantation ; Muscular Dystrophies/*surgery ; Transplantation/adverse effects
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 8
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    Residual vision in a scotoma: implications for blindsight (1992)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1992-11-27
    Beschreibung: Blindsight, the ability of some blind patients to describe attributes of stimuli they have no conscious awareness of seeing, has been attributed to a secondary (retinotectal) visual pathway. However, it has also been proposed that blindsight could be due to residual function within the primary (geniculostriate) visual pathway. Data have now been obtained that support the second alternative. With an image stabilizer ensuring the accurate retinal placement of stimuli, dense visual field mapping was carried out with a hemianopic patient. This perimetry revealed, embedded in the patient's scotoma, an isolated 1-degree island of residual vision that was not disclosed by conventional perimetric methods. Stimuli presented to this island could be detected and discriminated, although the subject reported he did not see them. The existence of this island of vision implies a corresponding island of functioning cortex within the patient's lesion. Other instances of blindsight may be mediated by similar islands of functioning cortex.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Fendrich, R -- Wessinger, C M -- Gazzaniga, M S -- P01 NS1778-10/NS/NINDS NIH HHS/ -- New York, N.Y. -- Science. 1992 Nov 27;258(5087):1489-91.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Center for Neuroscience, University of California, Davis 95616.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1439839" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Eye Movements/physiology ; Hemianopsia/*physiopathology ; Humans ; Male ; Middle Aged ; Scotoma/*physiopathology ; Sensitivity and Specificity ; Visual Cortex/physiopathology ; Visual Field Tests/methods ; Visual Fields/*physiology ; Visual Pathways/physiopathology
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 9
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    Programmed death of T cells in HIV-1 infection (1992)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1992-07-10
    Beschreibung: In human immunodeficiency virus (HIV) infection, functional defects and deletion of antigen-reactive T cells are more frequent than can be explained by direct viral infection. On culturing, both CD4+ and CD8+ T cells from asymptomatic HIV-infected individuals died as a result of programmed cell death (apoptosis). Apoptosis was enhanced by activation with CD3 antibodies. Programmed cell death, associated with impaired T cell reactivity, may thus be responsible for the deletion of reactive T cells that contributes to HIV-induced immunodeficiency.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Meyaard, L -- Otto, S A -- Jonker, R R -- Mijnster, M J -- Keet, R P -- Miedema, F -- New York, N.Y. -- Science. 1992 Jul 10;257(5067):217-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Clinical Viro-Immunology, Central Laboratory of The Netherlands Red Cross Blood Transfusion Service, Amsterdam.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1352911" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Acquired Immunodeficiency Syndrome/*pathology ; Antigens, CD/physiology ; Antigens, CD8/immunology ; CD4-Positive T-Lymphocytes/pathology ; Cell Death/physiology ; Cell Division/immunology ; Cells, Cultured ; HIV Envelope Protein gp120/physiology ; *Hiv-1 ; Humans ; Male ; Microscopy, Electron ; T-Lymphocytes/*pathology ; Zinc/pharmacology
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 10
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    X-ray laser microscopy of rat sperm nuclei (1992)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1992-10-19
    Beschreibung: The development of high brightness and short pulse width (〈 200 picoseconds) x-ray lasers now offers biologists the possibility of high-resolution imaging of specimens in an aqueous environment without the blurring effects associated with natural motions and chemical erosion. As a step toward developing the capabilities of this type of x-ray microscopy, a tantalum x-ray laser at 44.83 angstrom wavelength was used together with an x-ray zone plate lens to image both unlabeled and selectively gold-labeled dried rat sperm nuclei. The observed images show approximately 500 angstrom features, illustrate the importance of x-ray microscopy in determining chemical composition, and provide information about the uniformity of sperm chromatin organization and the extent of sperm chromatin hydration.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Da Silva, L B -- Trebes, J E -- Balhorn, R -- Mrowka, S -- Anderson, E -- Attwood, D T -- Barbee, T W Jr -- Brase, J -- Corzett, M -- Gray, J -- New York, N.Y. -- Science. 1992 Oct 9;258(5080):269-71.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Physics, University of California, Berkeley 94720.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1411525" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Cell Fractionation ; Cell Nucleus/*ultrastructure ; Chromatin/ultrastructure ; DNA/ultrastructure ; Epididymis/cytology ; Immunohistochemistry ; *Lasers ; Male ; Microscopy/*methods ; Rats ; Spermatozoa/*ultrastructure ; X-Rays
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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