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  • Nicotiana  (8)
  • Springer  (8)
  • Blackwell Publishing Ltd
  • 1980-1984  (8)
  • 1975-1979
  • 1984  (4)
  • 1982  (4)
Collection
Keywords
Publisher
  • Springer  (8)
  • Blackwell Publishing Ltd
Years
  • 1980-1984  (8)
  • 1975-1979
Year
  • 1
    Electronic Resource
    Electronic Resource
    Nicotiana chloroplast genome (1984)
    Springer
    Theoretical and applied genetics 67 (1984), S. 185-193 
    Details
    ISSN: 1432-2242
    Keywords: Nicotiana ; Chloroplast DNA ; Deletion ; Restriction ; Hot spot ; Inverted repeats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A physical map containing six restriction sites of the Nicotiana tabacum chloroplast genome, together with the BamHI maps of N. tabacum, N. otophora and N. knightiana, and the SmaI maps of N. acuminata, N. plumbaginifolia, N. langsdorffii, N. otophora, N. tabacum, N. tomentosiformis and N. knightiana was constructed. In Nicotiana chloroplast genomes, the most frequently observed variations are point mutations. Deletions are also detected. Most of the observed changes are confined to one area of the large single copy region, which is designated as the “hot spot”. Based on the evidence obtained from Nicotiana chloroplast genomes, an origin of the inverted repeats in this genus is proposed. We suggest that the inverted repeats represent a vestige of what were once two identical, complete chloroplast genomes joined together in a head-to-head and tail-to-tail fashion, and that deletions generated the current chloroplast genome organization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00317030
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  • 2
    Electronic Resource
    Electronic Resource
    Nicotiana chloroplast genome (1984)
    Springer
    Theoretical and applied genetics 67 (1984), S. 333-336 
    Details
    ISSN: 1432-2242
    Keywords: Nicotiana ; Chlamydomonas ; Chloroplast DNA ; RuBPCase ; E. coli ; B. subtilis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary RuBPCase, the enzyme responsible for carboxylation and oxidation of RuBP in a wide variety of photosynthetic organisms, is the major protein found in the chloroplast. Here we present the first evidence for direct expression in E. coli and B. subtilis of tobacco and Chlamydomonas ct-DNA sequences coding for the LS of RuBPCase as demonstrated by a simple in situ immunoassay.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00272870
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  • 3
    Electronic Resource
    Electronic Resource
    Metabolism of p-fluorophenylalanine in p-fluorophenylalanine sensitive and resistant tobacco cell cultures (1982)
    Springer
    Planta 155 (1982), S. 244-250 
    Details
    ISSN: 1432-2048
    Keywords: Cell suspension culture ; p-Fluorophenylalanine (metabolism of) ; Nicotiana ; Phenylalanine ammonia-lyase ; Resistant cell
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The metabolism of D- and L-p-fluorophenylalanine (PFP) in DL-PFP resistant and sensitive tobacco cell cultures (Nicotiana tabacum), cell lines TX4 and TX1, respectively, has been compared. The amino acid analogue was taken up at a lower rate by the resistant cell line TX4. Incorporation of PFP into protein was also considerably reduced in TX4 cells, compared to TX1 cells. This, however, resulted mainly from a diminished availability of PFP due to a more rapid conversion of PFP by TX4 cells. TX1 cells and TX4 cells converted PFP qualitatively in the same way. The only detectable metabolite of D-PFP was N-malonyl-D-PFP, while all metabolites of L-PFP were identified as sequent products of the initial deamination of L-PFP by the enzyme phenylalanine ammonia-lyase (PAL). As TX4 cells were endowed with higher PAL-activity than TX1 cells, the resistant cells were able to metabolize L-PFP more rapidly to give, e.g., p-fluorocinnamoyl glucose ester and p-fluorocinnamoyl putrescine. In the presence of the specific PAL-inhibitor α-aminooxy-β-phenylpropionic acid TX4 cells were slightly more sensitive to PFP. This suggests that the better “detoxification” contributes to the acquired resistance. The use of PFP as specific indicator for cell lines with increased PAL-activity, and hence increased levels of phenolic compounds, is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00392723
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  • 4
    Electronic Resource
    Electronic Resource
    Nicotiana chloroplast genome III. Chloroplast DNA evolution (1982)
    Springer
    Theoretical and applied genetics 61 (1982), S. 73-79 
    Details
    ISSN: 1432-2242
    Keywords: Nicotiana ; Chloroplast DNA ; Restriction fragments ; Deletion ; Evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Nicotiana chloroplast genomes exhibit a high degree of diversity and a general similarity as revealed by restriction enzyme analysis. This property can be measured accurately by restriction enzymes which generate over 20 fragments. However, the restriction enzymes which generate a small number (about 10) of fragments are extremely useful not only in constructing the restriction maps but also in establishing the sequence of ct-DNA evolution. By using a single enzyme, Sma I, a elimination and sequential gain of its recognition sites during the course of ct-DNA evolution is clearly demonstrated. Thus, a sequence of ct-DNA evolution for many Nicotiana species is formulated. The observed changes are all clustered in one region to form a “hot spot” in the circular molecule of ct-DNA. The mechanisms involved for such alterations are mostly point mutations but inversion and deficiency are also indicated. Since there is a close correlation between the ct-DNA evolution and speciation in Nicotiana a high degree of cooperation and coordination betwen organellar and nuclear genomes is evident.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00261515
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  • 5
    Electronic Resource
    Electronic Resource
    Comparison of Nicotiana tabacum and Nicotiana nesophila hybrids produced by ovule culture and protoplast fusion (1982)
    Springer
    Theoretical and applied genetics 62 (1982), S. 193-198 
    Details
    ISSN: 1432-2242
    Keywords: Nicotiana ; Somatic hybrids ; Protoplast fusion ; Ovule culture ; Genetic variability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Somatic hybrids were produced between Nicotiana tabacum and N. nesophila, two species incapable of conventional sexual hybridization. Sexual hybrids, though, could be produced between these two species by using ovule culture only when N. nesophila was female. Clones of somatic hybrids were compared with sexual hybrids. Statistically significant variation was observed between clones, but not between sexual hybrids, for pollen viability, flower morphology, leaf morphology, and trichome density. As all clones of somatic hybrids have 96 chromosomes, the variability could not be explained by interclonal variation in chromosome number. Variation between somatic hybrids could be the result of cytoplasmic segregation or recombination, mitotic recombination or small chromosomal rearrangements prior to plant regeneration. Variation between clones could be exploited as these interspecies hybrids are now being used to incorporate disease resistance into cultivated tobacco.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276236
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  • 6
    Electronic Resource
    Electronic Resource
    Valine resistant plants derived from mutated haploid and diploid protoplasts of Nicotiana sylvestris and N. tabacum (1982)
    Springer
    Theoretical and applied genetics 64 (1982), S. 51-58 
    Details
    ISSN: 1432-2242
    Keywords: Haploid protoplast ; Diploid protoplasts ; Metabolic mutants ; U.V. sensitivity ; Nicotiana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Protoplasts were derived from haploid and diploid Nicotiana sylvestris and N. tabacum. Exposure of the protoplasts to mutagenic doses of ultraviolet (U.V.) radiation prior to two selection rounds in the presence of 4 mM (or 5 mM) and 8 mM of valine, respectively, was required to obtain cell lines with persistent valine resistance. Such lines were obtained from haploid and diploid N. sylvestris protoplasts as well as from haploid protoplasts of N. tabacum but not from (1.8 × 107) diploid N. tabacum protoplasts. The ratio between number of verified valine-resistant cell lines and the initial number of U.V. exposed protoplasts enabled the estimation of the following order of mutation frequency: haploid N. sylvestris 〉 haploid N. tabacum 〉 diploid N. sylvestris. Plants which retained the valine resistance and transmitted it to their sexual progeny were derived from the resistant cell lines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00303650
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  • 7
    Electronic Resource
    Electronic Resource
    Generation of heteroplastidic Nicotiana cybrids by protoplast fusion: analysis for plastid recombinant types (1984)
    Springer
    Theoretical and applied genetics 67 (1984), S. 491-497 
    Details
    ISSN: 1432-2242
    Keywords: Tobacco ; Chloroplast DNA ; Cybrids ; Male sterility ; Tentoxin resistance ; Plastid recombinants ; Nicotiana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Protoplasts of a mutant line of Nicotiana tabacum having a maternally-transmitted chlorophyll deficiency were fused with protoplasts of two alloplasmic-male-sterile Nicotiana lines by the “donor-recipient” technique. In both fusion experiments variegated plantlets were regenerated which were shown to contain cytoplasms of mixed chloroplast nature. This confirms that with the “donor-recipient” method one can obtain mixed cytoplasms of genetically different chloroplasts. We present a convenient system to assay for genetic recombination between chloroplasts by combining use of several cytoplasmic markers: vis. chlorophyll pigmentation, chloroplast DNA restriction patterns, tentoxin resistance and male sterility. Within the limits of the experiment no recombinant types were recovered.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00264891
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  • 8
    Electronic Resource
    Electronic Resource
    Analysis of chromosomes and ribosomal RNA genes in parasexual hybrids ofNicotiana (1984)
    Springer
    Protoplasma 121 (1984), S. 228-231 
    Details
    ISSN: 1615-6102
    Keywords: Nicotiana ; Somatic hybrids ; Chromosome ; Ribosomal ; RNA genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The frequency of hybrid formation among calli developed from protoplasts ofNicotiana glauca andN. langsdorffii which had been treated with polyethylene glycole — Ca++ was determined. Approximately 5% of calli were proven to be somatic hybrids regardless selection pressure. Observation of chromosomes of selected hybrids showed variation in number ranging from 57–128, whereas parents contained 34–48. Analysis of ribosomal RNA genes (rDNA) confirmed every hybrid clone to contain rDNA from both parents.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01282316
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