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Analysis of chromosomes and ribosomal RNA genes in parasexual hybrids ofNicotiana

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Summary

The frequency of hybrid formation among calli developed from protoplasts ofNicotiana glauca andN. langsdorffii which had been treated with polyethylene glycole — Ca++ was determined. Approximately 5% of calli were proven to be somatic hybrids regardless selection pressure.

Observation of chromosomes of selected hybrids showed variation in number ranging from 57–128, whereas parents contained 34–48. Analysis of ribosomal RNA genes (rDNA) confirmed every hybrid clone to contain rDNA from both parents.

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Author notes

  1. D. S. Brar

    Present address: College of Agriculture, Punjab Agricultural University, 141004, Ludhiana, India

Authors and Affiliations

  1. Akitsu Branch, Fruit Tree Research Station, Akitsu, 729-24, Hiroshima, Japan

    S. Kobayashi

  2. Institute of Biological Sciences, University of Tsukuba, Sakura-mura, 305, Ibaraki, Japan

    D. S. Brar, M. Ono, H. Uchimiya & H. Harada

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  1. D. S. Brar
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  2. M. Ono
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  3. S. Kobayashi
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  4. H. Uchimiya
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  5. H. Harada
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Brar, D.S., Ono, M., Kobayashi, S. et al. Analysis of chromosomes and ribosomal RNA genes in parasexual hybrids ofNicotiana . Protoplasma 121, 228–231 (1984). https://doi.org/10.1007/BF01282316

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  • DOI: https://doi.org/10.1007/BF01282316

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