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  • Phaseolus  (6)
  • RNA  (4)
  • Wheat
  • Springer  (13)
  • American Association of Petroleum Geologists (AAPG)
  • 1980-1984  (13)
  • 1980  (13)
Collection
Keywords
Publisher
  • Springer  (13)
  • American Association of Petroleum Geologists (AAPG)
Years
  • 1980-1984  (13)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Changing cell-wall compositions in hypocotyls of dark-grown bean seedlings (1980)
    Springer
    Planta 149 (1980), S. 209-212 
    Details
    ISSN: 1432-2048
    Keywords: Cell elongation ; Cell wall ; Glucan ; Phaseolus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Hypocotyls of dark-grown 6-day-old seedlings of Phaseolus vulgaris L. proved to be sufficiently homogeneous to permit studies relating the rate of cell elongation to the composition of the primary cell walls. Whereas the levels of cellulose and uronic acids remained practically constant during and after cell extension, all other components showed major or minor changes. Cell-wall protein, as such, decreased by more than 50%, but indications are that hydroxyproline-rich glycoprotein increased with a decreasing rate of cell elongation, concomitant with a rise in the degree of arabinosylation of wall-bound hydroxyproline. As cell elongation slowed down, non-cellulosic glucose accumulated, presumably in the form of a β-(1–4)glucan closely associated with cellulose. These findings confirm the notion that the primary cell wall is a highly dynamic structure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384555
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  • 2
    Electronic Resource
    Electronic Resource
    Cell length, light and14C-labelled indol-3yl-acetic acid transport inPisum satisum L. andPhaseolus vulgaris L (1980)
    Springer
    Planta 149 (1980), S. 327-331 
    Details
    ISSN: 1432-2048
    Keywords: Auxin transport ; Cell length ; Light and auxin transport ; Phaseolus ; Pisum ; Transport (auxin)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The putative auxin-transporting cells of the intact herbaceous dicotyledon are the young, differentiating vascular elements. The length of these cells was found to be considerably greater in dwarf (Meteor) than in tall (Alderman) varieties ofPisum sativum L., and to be greater in etiolated than in light-grown plants ofP. sativum cv Meteor andPhaseolus vulgaris L. cv Mexican Black. Under given light conditions during transport these large differences in cell length did not influence the shapes of the transport profiles or the velocity of transport of14C-labelled indol-3yl-acetic acid (IAA) applied to the apical bud. However, in both etiolated and light-grown bean and dwarf pea plants the velocity of transport in darkness was ca. 25% lower than that in light. Under the same conditions of transport velocities in bean were about twice those observed in the dwarf pea. Exposure to light during transport increased the rate of export of14C from the labelled shoot apex in green dwarf pea plants but not in etiolated plants. The light conditions to which the plants were exposed during growth and transport had little effect on the rates of uptake of IAA from the applied solutions. The results indicate that the velocity of auxin transport is independent of the frequency of cell-to-cell interfaces along the transport pathway and it is suggested that in intact plants auxin transport is entirely symplastic.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00571165
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  • 3
    Electronic Resource
    Electronic Resource
    Characterisation of the storage protein subunits synthesised in vitro by polyribosomes and RNA from developing pea (Pisum sativum L.) (1980)
    Springer
    Planta 148 (1980), S. 49-56 
    Details
    ISSN: 1432-2048
    Keywords: Legumin ; Pisum ; Protien synthesis ; RNA ; Storage protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Evidence is presented to show that legumin, the major storage protein in Pisum, is synthesised in vitro by the wheat germ and reticulocyte lysate systems, from polyribosomes and mRNA isolated from developing pea seeds. While legumin isolated from mature pea seeds consists of 40,000 and 20,000 MW subunits, the in vitro legumin is synthesised as a 60,000 MW precursor consisting of covalently linked 40,000 and 20,000 MW subunits. The implications of these findings are discussed in relationship to studies with other systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385441
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  • 4
    Electronic Resource
    Electronic Resource
    Studies on ethylene binding by cell-free preparations from cotyledons of Phaseolus vulgaris L. (1980)
    Springer
    Planta 148 (1980), S. 397-406 
    Details
    ISSN: 1432-2048
    Keywords: Ethylene binding ; Hormone binding ; Phaseolus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The preparation is described of a cell-free system from developing cotyledons of Phaseolus vulgaris cv. Canadian Wonder which is capable of binding ethylene. The binding is saturable and the apparent dissociation constant for ethylene is 6.4·10-10 M in solution. The binding site is associated with subcellular particles and treatment with Triton X-100 results in substantial solubilisation of the activity. The kinetics of association and dissociation of the ligand and the binding site are described. The system is heat labile and binding activity is diminished by treatment with some proteolytic enzymes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388129
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  • 5
    Electronic Resource
    Electronic Resource
    Studies on ethylene binding by cell free preparations from cotyledons of Phaseolus vulgaris L. (1980)
    Springer
    Planta 148 (1980), S. 407-411 
    Details
    ISSN: 1432-2048
    Keywords: Ethylene binding ; Hormone binding ; Phaseolus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Various protein reactive agents such as dithioerythritol, dithiothreitol, mercaptoethanol and p-chloromercuribenzoate inhibit binding of ethylene to cell free preparations of Phaseolus vulgaris L. The effect of the thiols is partially reversed by treatment with diamide; occupation of the binding site by ligand diminishes the inhibition caused by p-chloromercuribenzoate but not that caused by thiols. Growth regulators other than ethylene do not affect binding. Physiologically active structural analogues of ethylene competitively inhibit binding of the growth regulator and their relative effectiveness in the cell free system closely resembles that in developmental processes controlled ethylene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388130
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  • 6
    Electronic Resource
    Electronic Resource
    RNase-sensitive DNA polymerase activity in cell fractions and mutants of Neurospora crassa (1980)
    Springer
    Biochemical genetics 18 (1980), S. 743-753 
    Details
    ISSN: 1573-4927
    Keywords: RNase-senitive DNA polymerase activity ; RNA ; DNA ; Neurospora crassa ; differential DNA polymerase activity ; cell fractions and mutants of N. crassa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract RNase-sensitive DNA polymerase activity (RSDP) was tested in different cell fractions of Neurospora crassa cell types and its morphological mutants. This RSDP was found localized in the microsomal pellet fraction and absent in the purified nuclear pellets isolated from different N. crassa cell types: conidia, germinated conidia, and mycelia. This enzyme is capable of synthesizing a DNA product only in the presence of all four deoxyribonucleoside-5′-triphosphates and Mg2+. Removal of RNA from the pellet fraction by RNase strongly inhibited the DNA synthesis. The endogenous synthesis of DNA in the microsomal pellet fraction was associated with the formation of an RNA:DNA hybrid as analyzed by Cs2SO4 equilibrium density gradient centrifugation. The DNA product after alkali hydrolysis hybridizes with the RNA isolated from the same pellet fraction, as analyzed by elution from hydroxylapatite column at 60 C. This DNA product did not hybridize with poly(A). A few mutants tested showed this RNase-sensitive DNA polymerase activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00484590
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  • 7
    Electronic Resource
    Electronic Resource
    Meiosis and fertility of interspecific hybrids between Phaseolus vulgaris L. and P. acutifolius A. Gray (1980)
    Springer
    Theoretical and applied genetics 57 (1980), S. 59-64 
    Details
    ISSN: 1432-2242
    Keywords: Phaseolus ; Interspecific hybridization ; Meiosis ; Fertility ; Beans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Meiosis and fertility of interspecific hybrids obtained from reciprocal crosses between Phaseolus vulgaris and P. acutifolius were examined. Bivalents as well as univalents were found at Metaphase I. The majority of the microsporocytes had four or more univalents and the average was 6.3 univalents per cell. The average number of lagging chromosomes at Anaphase I was 2.3 per cell and the most frequent chromosome distribution at late Anaphase I was 10–12. The lower than expected number of lagging chromosomes as compared with the number of univalents at Metaphase I suggests the possible occurrence of precocious separation of bivalents. The male fertility as measured by pollen stainability was 17%, however, the frequency of pollen germination in selfing was 3.5%. Upon selfing of the interspecific hybrids, no dividing embryos were found even though 7 and 26% of the ovules were fertilized at 12 hours and four days after pollination. In backcrosses to P. vulgaris (male), 6 and 20% of the ovules were fertilized and 0 and 4% of the ovules contained dividing embryos at the same sampling times. When P. acutifolius was the male parent, respective values were 8 and 31% for fertilization and 0 and 13% for ovules with dividing embryos. The frequencies of backcross embryos recovered at 14–26 days were in agreement with the frequencies of dividing embryos at four days. The ability to obtain backcross plantlets demonstrates the feasibility to further utilize interspecific hybrids for the improvement of P. vulgaris
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276402
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  • 8
    Electronic Resource
    Electronic Resource
    Meiosis and fertility of interspecific hybrids betweenPhaseolus vulgaris L. andP. acutifolius A. Gray (1980)
    Springer
    Theoretical and applied genetics 57 (1980), S. 59-64 
    Details
    ISSN: 1432-2242
    Keywords: Phaseolus ; Interspecific hybridization ; Meiosis ; Fertility ; Beans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Meiosis and fertility of interspecific hybrids obtained from reciprocal crosses betweenPhaseolus vulgaris andP. acutifolius were examined. Bivalents as well as univalents were found at Metaphase I. The majority of the microsporocytes had four or more univalents and the average was 6.3 univalents per cell. The average number of lagging chromosomes at Anaphase I was 2.3 per cell and the most frequent chromosome distribution at late Anaphase I was 10–12. The lower than expected number of lagging chromosomes as compared with the number of univalents at Metaphase I suggests the possible occurrence of precocious separation of bivalents. The male fertility as measured by pollen stainability was 17%, however, the frequency of pollen germination in selfing was 3.5%. Upon selfing of the interspecific hybrids, no dividing embryos were found even though 7 and 26% of the ovules were fertilized at 12 hours and four days after pollination. In backcrosses toP. vulgaris (male), 6 and 20% of the ovules were fertilized and 0 and 4% of the ovules contained dividing embryos at the same sampling times. WhenP. acutifolius was the male parent, respective values were 8 and 31% for fertilization and 0 and 13% for ovules with dividing embryos. The frequencies of backcross embryos recovered at 14–26 days were in agreement with the frequencies of dividing embryos at four days. The ability to obtain backcross plantlets demonstrates the feasibility to further utilize interspecific hybrids for the improvement ofP. vulgaris
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00745030
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  • 9
    Electronic Resource
    Electronic Resource
    Purification of lumicolchicine for use in studies of plant development (1980)
    Springer
    Protoplasma 103 (1980), S. 105-114 
    Details
    ISSN: 1615-6102
    Keywords: Colchicine ; Lumicolchicine ; Microtubules ; Mitosis ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Lumicolchicine was purified by preparative thin-layer chromatography. Tests for purity were ultraviolet absorption spectrophotometry, analytical thin-layer chromatography, and a bioassay using wheat roots. Wheat roots treated for 3 days with 10−3 M lumicolchicine showed no c-mitosis, but had reduced growth compared with controls.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01276669
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  • 10
    Electronic Resource
    Electronic Resource
    Nuclear and cytoplasmic RNA in visual cortical neurons of adult rats during visual deprivation and photic stimulation (1980)
    Springer
    Bulletin of experimental biology and medicine 90 (1980), S. 1455-1457 
    Details
    ISSN: 1573-8221
    Keywords: neuron ; glia ; RNA ; visual and motor cortex ; visual deprivation ; photic stimulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00838834
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