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  • Cell & Developmental Biology  (104)
  • FLUID MECHANICS AND HEAT TRANSFER  (104)
  • 1995-1999
  • 1980-1984  (208)
  • 1940-1944
  • 1982  (139)
  • 1980  (69)
Collection
Keywords
Publisher
Years
  • 1995-1999
  • 1980-1984  (208)
  • 1940-1944
Year
  • 1
    Electronic Resource
    Electronic Resource
    Perturbation of the switch-on of transcriptase activity in intermediate subviral particles from reovirus (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 112 (1982), S. 10-18 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Intermediate subviral particles (ISVP) derived from reovirus represent a simple model system for the switch-on of transcriptase function. In such particles the endogenous transcriptase is present in a switched-off form, one step removed from the switched-on state. Switch-on of transcriptase function is an active process in this system and can be triggered by K+ ions. A variety of agents which affect gene expression in cells were tested for an effect on switch-on in ISVP. Marked effects on switch-on in ISVP were observed with a diverse group of test agents, including DMSO and other solvents, BUdR, TdR, caffeine, theophylline, and temperature. The correlation in response between ISVP and cells suggests that the ISVP system may be useful as a model for studying the biochemical mechanisms underlying the perturbative effects of such agents on gene expression in cells.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041120104
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    Paper (German National Licenses)
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  • 2
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    Mean flowfields in axisymmetric combustor geometries with swirl (1982)
    In:  Other Sources
    Details
    Publication Date: 2019-07-13
    Description: Six flowfield configurations are investigated with sidewall angles of 90 and 45 deg, and swirl vane angles of 0, 45, and 70 deg. It is found that central recirculation zones occur for the swirling flow cases investigated, which extend from the inlet to x/D = 1.7, where x is the axial polar coordinate, and D is the test section diameter. Five-hole pitot probe pressure measurements are used to determine time-mean velocities, and corresponding flow situations are predicted and compared to results of experimental data. Excellent agreement is found for the nonswirling flow, although poor agreement is found for swirling flow cases, especially near the inlet. The discrepancy is attributed to the lack of realism in the turbulence model, and/or to inaccurate specification of time-mean velocity and turbulence energy distributions at the inlet.
    Keywords: FLUID MECHANICS AND HEAT TRANSFER
    Type: AIAA PAPER 82-0177 , Aerospace Sciences Meeting; Jan 11, 1982 - Jan 14, 1982; Orlando, FL
    Format: text
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    NASA TECHNICAL REPORTS
  • 3
    Electronic Resource
    Electronic Resource
    Setiferous structures of male coleoptera (1982)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 173 (1982), S. 43-72 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A scanning electron microscopy study was made of the male setiferous sex patches and analogous structures in 11 families of Coleoptera (Anthribidae, Bruchidae, Ciidae, Cleridae, Coccinellidae, Dermestidae, Leiodidae, Ptinidae, Staphylinidae, Tenebrionidae, and Ostomatidae). These secondary sexual characters appear to have several features in common including relatively long, often ridged, setae, cuticular ducts (frequently cribriform pore plates), and the production of a secretion. It is suggested that these structures may all be concerned with the production, release, and dissemination of pheromones.
    Additional Material: 21 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051730106
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  • 4
    Electronic Resource
    Electronic Resource
    Sorbic acid-induced differences in the ultrastructural development of oocytes in the microbially ectosymbiotic female of Xyleborus ferrugineus (fabr.) (coleoptera, scolytidae) (1982)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 173 (1982), S. 313-324 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The ovaries of the beetle Xyleborus ferrugineus reared on standard sawdust diet with an without 0.08% sorbic acid added were examined for differences in ultrastructural development of the oocytes. Indications of vigorous yolk deposition are an extensive rough-surfaced endoplasmic reticulum (RER), numerous electron-dense secretory vesicles and a prominent nucleus in associated follice cells, and extremely electron-opaque material in the interfollicular cell spaces and the perioocytic area. After 6 days of feeding without added sorbic acid, a mature terminal oocyte is present in one of the two ovaries. This terminal oocyte at this mature stage contains yolk spheres and lipid bodies. However, the most mature oocyte in beetles reared on the standard sawdust diet to which 0.08% sorbic acid was added remained at a previtellogenic stage after 6 days of feeding. Titers of ecdysone in 6-day-old adult females reared on standard sawdust without and with 0.08% sorbic acid added were 534.64 ± 20.93 S.D. pg/mg and 39.94 ± 14.71 S.D. pg/mg body weight, respectively.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051730308
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  • 5
    Electronic Resource
    Electronic Resource
    The bursa cloacae (Fabricii) of struthioniforms in comparison with the bursa of other birds (1982)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 172 (1982), S. 123-138 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The late embryonic and postembryonic genesis of the bursa cloacae (Fabricii) of struthioniforms and other birds is described and discussed. The bursa of ostrich and emu is a wall organ of the caudal cloacal chamber. The bursa of rhea is, like the bursa of Gallus, a cranial appendix of the proctodeum. Lobuli bursales of struthioniforms are composed of a peripheral pars lymphoepithelialis (PLE) and a central pars lymphoreticularis (PLR). By contrast, lobuli bursales of Gallus are composed of a peripheral PLR and a central PLE. The fine structure of the bursa of struthioniforms is described. Other than in Gallus, the apical cell association of the PLE of struthioniforms shows secretory granules. This study thus far does not answer in detail the question of how the imprinting mechanism of the B-lymphocytes operates. It is assumed that they are imprinted in the PLE. Postcapillary venules in the PLR are responsible for the transport of B-lymphocytes. Hormonal bursectomies have been made to get information about the involution of the bursa of struthioniforms. In these species, involution means a gradual metaplasia while in Gallus it means a complete degeneration of the bursa.
    Additional Material: 16 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051720111
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  • 6
    Electronic Resource
    Electronic Resource
    Physicochemical properties of 21S dynein ATPase binding to A and B subfiber microtubules (1982)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 2 (1982), S. 113-119 
    Details
    ISSN: 0886-1544
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970020722
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  • 7
    Electronic Resource
    Electronic Resource
    Meeting report (1980)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 1 (1980), S. 159-162 
    Details
    ISSN: 0886-1544
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970010112
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  • 8
    Electronic Resource
    Electronic Resource
    Physarum myosin light chain binds calcium (1980)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 1 (1980), S. 63-71 
    Details
    ISSN: 0886-1544
    Keywords: Physarum polycephalum ; myosin light chains ; polyacrylamide gel electrophoresis ; calcium ; cytoplasmic streaming ; actomyosin ATPase regulation ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Myosin from the slime mold Physarum polycephalum contains three sizes of polypeptides: a heavy chain and two light chains, LC-1 and LC-2. Using a simple qualitative test for calcium binding by comparing electrophoretic migration of the polypeptides in sodium dodecy1 sulfate (SDS) acrylamide gels in the presence and absence of calcium, we have found that Physarum myosin light chain LC-2 migrates with an apparent molecular weight of 16,900 daltons in the presence of the metal ion chelator ethylene glycol bis (B-aminoethyl ether) N,N′-tetraacetic acid (EGTA). However, if calcium chloride is added to the sample prior to electrophoresis, the apparent molecular weight decreases to 16,100. Lanthanide and cadmium ions, but not magnesium, can substitute for calcium. Because the ionic radii of Ca2+, La3+, and Cd2+ are almost identical, we conclude that Physarum myosin LC-2 possesses a very size-specific binding site for calcium. Physarum myosin LC-1 and the heavy chain give no evidence for binding calcium by this test. Since cytoplasmic streaming in the plasmodium of Physarum requires calcium, our evidence indicates that the calcium-binding property of Physarum myosin LC-2 may be important in regulating the production of force by actomyosin in the ectoplasm. Unexpectedly, the myosin light chain in Physarum capable of binding calcium, LC-2, is the essential light chain, while LC-1 is a member of the regulatory class of myosin light chains [V. T. Nachmias, personal communication]. Until now, essential myosin light chains have not been shown to have high affinity divalent cation binding sites. This means a new version of the myosin-based model for actomyosin regulation by calcium may be required to explain cytoplasmic movement in Physarum, and perhaps in other motile systems involving cytoplasmic myosins as well.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970010106
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  • 9
    Electronic Resource
    Electronic Resource
    Comparative studies on human placental insulin and basic somatomedin receptors (1982)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 20 (1982), S. 283-292 
    Details
    ISSN: 0730-2312
    Keywords: insulin receptor ; basic somatomedin receptor ; human placenta ; peptide maps ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: The disuccinimidy! suberate, affinity-labeling procedure, and proteolytic mapping techniques have been employed to characterize further the human placental receptors for insulin and basic somatomedin. Electrophoretic analysis of the basic somatomedin receptor, selectively crosslinked to 125I basic somatomedin in the presence of excess native insulin revealed, under reducing conditions, major labeled constituents of 270-280 and 125-140 kd, substantiating our previous work employing a photoaffinity labeling reagent. Affinity labeling also demonstrated the presence of less intensely labeled components with apparent molecular weights of 40 and 45 kd but failed to reveal a distinct 90- to 100-kd species observed in parallel experiments with insulin. In the absence of β-mercaptoethanol, all components specifically labeled with 125I basic somatomedin migrated in the 300- to 400-kd range. In comparison, selective affinity labeling of the insulin receptor in the presence of excess native basic somatomedin revealed components, upon electrophoresis under reducing conditions, with apparent molecular weights of 270-280, 125-140, 90-100, and 40 kd. The major insulin-labeled component (125-140 kd) comigrated with the major constituent (125-140 kd) selectively labeled with basic somatomedin. When digestion was performed prior to solubilization, chymotryptic and tryptic proteolysis of the membrane-localized selectively labeled insulin, and basic somatomedin receptors yielded quite similar gel electrophoretic maps. However, when digestion was done subsequent to solubilization, chymotryptic and tryptic proteolysis of selectively labeled insulin and basic somatomedin receptors solubilized in SDS yielded similar but not identical gel electrophoretic maps. We conclude that the receptors for basic somatomedin and insulin are highly homologous structures with respect to their disulfide crosslinked composition, and with respect to the size of the major components detected by selective affinity-labeling procedures. Nevertheless, the detection of electrophoretically distinct labeled receptor components upon analysis of specifically labeled intact or proteolytically digested receptors points to subtle differences between the polypeptide compositions of the two receptors.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240200308
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  • 10
    Electronic Resource
    Electronic Resource
    Altered cell cycle progression and aberrant mitosis in adenovirus-infected rodent cells (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 111 (1982), S. 89-96 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Actively growing mouse or rat embryo cells suffered structural chromosome damage, mitotic anomalies, and polyploidy after infection by human adenovirus type 5. Chromosome damage required expression of one or more early viral genes and showed regular periodicity in its frequency. The growth cycle time of some of the infected cells was reduced by about 5 hours due to a decrease in G1, and the interval between successive waves of chromosome damage corresponded to this reduced cycle time. After infection there was a decrease in cells with G1 DNA content and an increase in cells with G2 diploid, aneuploid, and polyploid DNA contents. We suggest these effects are due to the expression in semipermissive cells of early viral gene(s), whose function in productive infection in vivo is to alter cell cycle controls in order to maximize the number of cells able to replicate viral DNA and the time such cells spend in DNA replication.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041110114
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