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  • 1960  (15)
  • 1
    ISSN: 1573-4986
    Keywords: influenza virus ; hemagglutinin ; glycoprotein glycans ; oligosaccharide structure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Fowl plague virus, strain Dutch, was metabolically labeled withd-[2-3H]mannose, or withd-[6-3H]glucosamine, and the small subunit (HA2; 0.8 mg in total) of the viral hemagglutinin was isolated by preparative sodium dodecylsulfate-polyacrylamide gel electrophoresis. After proteolytic digestion, the radioactive oligosaccharides were sequentially liberated from the glycopeptides by treatment with different endo-β-N-acetylglucosaminidases and with peptide:N-glycosidase or, finally, by hydrazinolysis. In this manner, four groups of glycans could be obtained by consecutive gel filtrations and were subfractionated by HPLC. The structures of the individual oligosaccharides were analyzed by micromethylation, by acetolysis or by digestion with exoglycosidases. The major species amongst the high mannose glycans at Ans-406 of the viral glycopolypeptide were found to be Manα1-2Manα1-3(Manα1-2Manα1-6)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNacβ1-4GlcNAc and Manα1-3(Manα1-2Manα1-6)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc, while the complex glycans at Asn-478 are predominantly GlcNAcβ1-2Manα1-3(GlcNAcβ1-2Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAc (lacking, in part, one of the outerN-acetylglucosamine residues) and GlcNAcβ1-2Manα1-3(Galβ1-4GlcNAcβ1-2Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAc.
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  • 2
  • 3
    Electronic Resource
    Electronic Resource
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 86 (1987), S. 545-551 
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: The low frequency (500–80 cm−1) Raman spectra of gaseous methylcyclobutane, c-C4H7CH3, and methyl-d3-cyclobutane have been recorded. A series of Q branches beginning at 161 cm−1 for the light molecule and 154 cm−1 for the d3 compound with successive transitions falling to lower frequencies have been assigned to the ring puckering vibrations of both the low energy equatorial and high energy axial conformers. These data have been fitted to an asymmetric potential function of the form: V(cm−1)=(4.78±0.10)×105X4−(3.08±0.04)×104X2 +(2.18±0.1)×104X3 with an assumed reduced mass of 160 amu for the light compound. Utilizing this potential the difference between the puckering angles for the two conformers was calculated to be 4.3° with the equatorial conformer having the larger value of 20.7°. A similar potential was obtained for the d3 molecule. The energy difference between the equatorial and axial forms was found to be 247±20 cm−1 (706 cal/mol) and a barrier of 641±20 cm−1 (1.83 kcal/mol) was found for the interconversion. Experimental values for the ΔH of both the liquid (354±179 cm−1) and gas (295±75 cm−1) from relative intensities of different Raman band pairs over 85 and 37 °C temperature ranges, respectively, were found to be consistent with the value obtained from the potential function. These results are compared to the corresponding quantities for similar molecules.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Astrophysics and space science 131 (1987), S. 595-599 
    ISSN: 1572-946X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The discovery of magnetic field strengths in the range of 500 million Gauss in the objects Grw+70°8247 and PG1031+224 has given enormous impetus to investigations of magnetic white dwarf stars. We have determined intensities of Balmer transitions for B〉108 Gauss as a function of field and find strong oscillations of the transitions strengths, which are of importance to the quantitative analysis of the observed spectra and the interpretation of polarimetric measurements in these objects.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Czechoslovak journal of physics 37 (1987), S. 516-520 
    ISSN: 1572-9486
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The application of a special version of the nonlocal light-cone expansion to hadron processes with large momentum transfer is reviewed. The light-cone operators introduced in this approach may be considered as the relevant hadron operators: Their matrix elements between the vacuum and a one-hadron state coincide with the quark distribution amplitude, and their anomalous dimensions are unique extensions of the well known Brodsky-Lepage evolution kernels having also a simple relation to the Altarelli-Parisi kernels.
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  • 6
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 327 (1987), S. 321-324 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] For this study we used mixtures of 1-10% by weight each of surfactant and co-surfactant dissolved in salt solutions. The phase behaviour of such systems has recently been described by Benton and Miller4, who found that progressive addition of salt to an aqueous micellar dispersion (I) results in ...
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Chromosoma 11 (1960), S. 499-513 
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The first spermatocyte division has been investigated in Rhabdophaga batatas Walsh and Wachtliella persicariae L. It was shown that the course of this division cannot be described as mitosis, either “unipolar” or “modified bipolar”. Its essential features are: complete absence of pairing and the segregation of the chromosomes into two groups composed of 4 and 30-odd chromosomes. The segregation is accomplished by a peculiar expulsion of the group of 4 chromosomes from the main nucleus followed by an unequal cytokinesis. The spindle is absent and the nuclear membrane remains present during the entire course of the division. The second spermatocyte division occurs only in the smaller cell, which contains 4 chromosomes and bears all features of normal mitosis. For the description of the course of the first spermatocyte division and chromosome distribution, which occurs in this division, the terms “segregating division” and “monocentric configuration” were proposed. The available descriptions of the first spermatocyte division in other species of Cecidomyiidae have been analyzed. The pattern of this division in all so far investigated cecidomyiid species is very uniform and the terms “unipolar” or “modified bipolar” mitoses are here also inadequate. The cases were discussed in which the peculiar chromosome arrangements arise merely as a result of positive or negative interrelations between the centriole and the chromosome parts. With this as background, the monocentric configurations in Cecidomyiidae were explained in terms of an attraction between the centriole and centromeres, operating in one group, and a repulsion between the centriole and the chromosome ends acting in the second. The other so-called unipolar mitoses have been analyzed. It was shown that under this term at least three intrinsically different configurations have been described. For the cases of Sciara, Micromalthus debilis and Cecidomyiidae, the common denominator can be found: the chromosome movements in Sciara and M. debilis can also be explained as set forth above for Cecidomyiidae, i.e. in terms of two kinds of forces operating between the centriole and the chromosomes. The several spindle fibers present in Sciara and Micromalthus do not seem to be crucial for the chromosome movements.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Chromosoma 12 (1961), S. 233-247 
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary 1. Currently available data on chromosome elimination in Cecidomyiidae are still inadequate to support any definite conclusions regarding its immediate causes. It seems that some evidence on the mechanism of elimination could be obtained from observation of the behavior of E chromosomes in complete absence of spindle. To bring about such absence of spindle, ultraviolet microbeams would appear promising, since they have already been used to destroy spindles in cells of other organisms. 2. For irradiation the embryos of Rhabdophaga batatas Walsh were chosen. It was established that in this species 2 S+E=40 and 2 S (for female)=8. Elimination of 30-odd chromosomes regularly occurs in the fifth cleavage. Embryos were irradiated with 8μ and 16μ heterochromatic ultraviolet microbeams not only in this fifth cleavage but also in the fourth, in which elimination was never observed. The irradiated regions were small parts of somatic cytoplasm or small parts of germ cells. 3. The effects of irradiation were multifold: complete degeneration of nuclei in the vicinity of irradiated cytoplasm; disappearance of spindle and formation of quasi-rosettes; multipolar mitosis. The polar substance present in germ cells was often severely damaged and fragmented. All these effects are evidently indirect. 4. In mitoses thus deprived of spindles, the separation of chromosomes into daughter chromatids was suppressed but some of their active movements persisted. All chromosomes were gathered into a quasirosette, which afterwards divided into two quasi-rosettes. The daughter quasi-rosettes moved apart and formed telophase nuclei. In both irradiated divisions (fourth and fifth) E chromosomes behaved in exactly the same manner as S chromosomes; they showed the same kind of peculiar movement and were incorporated into telophase nuclei. Suppression of elimination of E chromosomes in the fifth division can be regarded as one more indirect effect of irradiation to be added to the list above. 5. These results show that elimination of E chromosomes does not result from failures in chromosomal spindle fibers or from intrinsic morphological defects in the centromeres. The fact that elimination can be suppressed by indirect action of ultraviolet light strongly supports the assumption that elimination occurs because of chemical changes in adjacent cytoplasm that are injurious to the centromeres of E chromosomes. Most probably the indirect action of ultraviolet interferes with the production of some substance that is normally injurious to the centromeres in the fifth division. 6. Since in Cecidomyiidae the general features of the metabolism are known to be stable up to the mid-blastoderm stage, it would appear that the chemical changes involved in elimination cleavage are probably quite specific.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Naturwissenschaften 47 (1960), S. 446-447 
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Naturwissenschaften 48 (1961), S. 623-623 
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
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