Publication Date:
2005-06-18
Description:
Rhizobial bacteria enter a symbiotic interaction with legumes, activating diverse responses in roots through the lipochito oligosaccharide signaling molecule Nod factor. Here, we show that NSP2 from Medicago truncatula encodes a GRAS protein essential for Nod-factor signaling. NSP2 functions downstream of Nod-factor-induced calcium spiking and a calcium/calmodulin-dependent protein kinase. We show that NSP2-GFP expressed from a constitutive promoter is localized to the endoplasmic reticulum/nuclear envelope and relocalizes to the nucleus after Nod-factor elicitation. This work provides evidence that a GRAS protein transduces calcium signals in plants and provides a possible regulator of Nod-factor-inducible gene expression.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kalo, Peter -- Gleason, Cynthia -- Edwards, Anne -- Marsh, John -- Mitra, Raka M -- Hirsch, Sibylle -- Jakab, Julia -- Sims, Sarah -- Long, Sharon R -- Rogers, Jane -- Kiss, Gyorgy B -- Downie, J Allan -- Oldroyd, Giles E D -- New York, N.Y. -- Science. 2005 Jun 17;308(5729):1786-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Departments of Disease and Stress Biology and Molecular Microbiology, John Innes Centre, Norwich NR4 7UH, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15961668" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Motifs
;
Amino Acid Sequence
;
Calcium/metabolism
;
Calcium Signaling
;
Calcium-Calmodulin-Dependent Protein Kinases/genetics/metabolism
;
Cell Nucleus/metabolism
;
Cloning, Molecular
;
Gene Expression Regulation, Plant
;
Genes, Plant
;
Lipopolysaccharides/*metabolism
;
Medicago/genetics/*metabolism/*microbiology
;
Molecular Sequence Data
;
Mutation
;
Oligonucleotide Array Sequence Analysis
;
Peas/genetics/metabolism
;
Plant Proteins/chemistry/genetics/*metabolism
;
Protein Structure, Tertiary
;
Recombinant Fusion Proteins/metabolism
;
*Signal Transduction
;
Sinorhizobium meliloti/*physiology
;
Symbiosis
;
Transcription Factors/chemistry/genetics/*metabolism
;
Transcription, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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