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  • 1
    ISSN: 1436-6215
    Keywords: purinreiche Nahrung ; Wachstum ; Metaboliten ; Harnsäuretransport ; Hund ; purine-rich diet ; growth ; metabolites ; uric acid transport ; dog
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Summary In a nutritional tolerance study 36 young dogs were fed over 52 weeks high or low purine diets at an average paired feeding intake of 0.6 and 80 mg purine-N/MJ per day. The high purine diet resulted in a significant decrease of growth rate by −23 % (Beagles) and −38 % (Dalmatians) and of feed efficiency (−29 and −42 %). The fasting levels of allantoin, uric acid and uracil in blood plasma were significantly increased. During the experiment a metabolic adaptation to the high purine diet decreased the plasma concentrations of uric acid, uracil and in part of allantoin. The high purine diet effected a significant increase of Km (2.5-fold) and of Vmax (1.6-fold) of uric transport through the erythrocyte membrane. The results documented disadvantageous effects of high purine nutrition during juvenile development.
    Notes: Zusammenfassung In einer Verträglichkeitsstudie erhielten 36 junge Hunde über 52 Wochen eine purinreiche oder purinarme Diät mit durchschnittlich 6 bzw. 80 mg Purin-N/MJ pro Tag bei gruppengleicher Aufnahme („paired feeding“). Die Hochpurin-Diät führte zu einer signifikanten Verminderung der Gewichtsentwicklung um −23% (Beagles) bzw. −38% (Dalmatiner) und der Futterverwertung (−29 bzw. −42%) sowie zu einer signifikanten Erhöhung der Nüchternwerte von Allantoin, Harnsäure und Uracil im Blutplasma. Während der Versuchsdauer machte sich eine metabolische Adaptation an die Hochpurin-Diät mit signifikanter Erniedrigung von Harnsäure, Uracil und teils auch Allantoin im Plasma bemerkbar. Die Hochpurin-Diät bewirkte eine signifikante Zunahme der Km (2,6fach) und Vmax (1,6fach) des Harnsäuretransports durch die Erythrozytenmembran. Die Ergebnisse belegen nachteilige Effekte purinreicher Ernährung während der Jugendentwicklung.
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  • 2
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    European journal of nutrition 36 (1997), S. 299-302 
    ISSN: 1436-6215
    Keywords: Energy retention ; protein retention ; fat retention ; growth ; body composition ; broilers ; Energieansatz ; Proteinansatz ; Fettansatz ; Wachstum ; Körperzusammensetzung ; Broiler
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Zusammenfassung Mit steigender Aufnahme erhöht sich der Körperfettgehalt und der Körperproteingehalt nimmt ab. Es wird oft angenommen, daß jede Zunahme im Ansatz mit mehr Fett und weniger Protein verbunden ist. Experimentelle Ergebnisse widerlegen jedoch diese Annahme. In zwei Experimenten mit männlichen Broilerhühnern wurden die Tiere auf einem Niveau von 60 % und 100 % der empfohlenen Energieaufnahme gefüttert. Die Körperzusammensetzung bei 1500 g zeigte, wie erwartet, daß sich bei steigenden Aufnahmen der Körperfettgchalt vergrößert und der Körperproteingehalt abnimmt. Fettund Proteinansatz waren linear mit der totalen Energieretention (ER) korreliert. Das bedeutet, daß jede Zunahme im Ansatz den gleichen Protein- und Fettgehalt besitzt. Wenn der Fettansatz gleich Null ist wird nur Protein, etwa 50 % des maximalen Ansatzes, retiniert. Wenn ER=O ist, wird Protein angesetzt und Fett mobilisiert. Energie-und N-Bilanzuntersuchungen bestätigen die konstante Zusammensetzung jeder Vergrößerung des Ansatzes. Die Ergebnisse beider Experimente zeigen, daß die ER aus zwei Komponenten besteht: einem basalen konstanten täglichen Proteinansatz und einer variablen zusätzlichen ER, die hauptsächlich aus Fett besteht. Der basale Proteinansatz beträgt etwa 50 % des maximalen Ansatzes. Mit steigenden Energieaufnahmen wird der basale Proteinansatz mit einer zusätzlichen Menge von Protein und Fett im konstanten Verhältnis ergänzt.
    Notes: Summary With increasing intakes the body fat content increases and that of protein decreases. It is most often assumed that this is brought about because each increment in retention contains more fat and less protein. Experimental results, however, showed that this explanation is not true. In two experiments male broiler chickens were fed at levels between 60 and 100 % of recommended energy intake. Body composition at 1500 g showed, as expected, that with increasing intakes body fat content increased and protein content decreased. Both fat and protein retention per day were linearly related to total energy retention (ER). This means that each increment in retention has the same protein and fat content. At zero fat retention only protein was retained, about 50 % of maximal retention. At zero ER protein was retained and fat mobilized. Energy and N balance experiments confirmed the constant composition of each increment in retention. The results of both experiments show that total ER consisted of two components: a basic constant daily protein retention and a variable additional ER, mainly consisting of fat. The basic protein retention is about half of maximal retention. With increasing energy intakes the basic protein retention is combined with an additional amount of protein and fat in a constant ratio.
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  • 3
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    European journal of nutrition 36 (1997), S. 332-335 
    ISSN: 1436-6215
    Keywords: Nutrient intake ; protein metabolism ; protein synthesis ; growth ; energy expenditures ; Nährstoffaufnahme ; Proteinstoffwechsel ; Proteinsynthese ; Wachstum ; Energieaufwand
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Zusammenfassung Eine Zunahme von fettfreiem Gewebe tritt auf, wenn die Proteinsynthese größer ist als der Proteinabbau. Obwohl während des Wachstums von der Geburt bis zur Reife die absoluten Proteinsynthese- und -abbauraten ansteigen, nehmen dagegen die fraktionellen Raten ab. Bcide Prozesse reagieren auf die Nährstoffaufnahme. Es gibt aber deutliche Unterschiede zwischen den verschiedenen Geweben. Protein, Kohlenhydrate und Fett können den Proteinansatz bei unreifen Tieren und Kindern stimulieren. Die zugrundeliegenden Mechanismen und die Energieaufwendungen scheinen jedoch unterschiedlich zu sein.
    Notes: Summary Lean tissue growth occurs when the rate of protein synthesis exceeds the rate of protein breakdown. Althoughabsolute rates of protein synthesis and breakdown rise during growth from birth to maturityfractional rates fall. Both these processes are sensitive to nutrient intake but responses to feeding vary greatly amongst different tissues. Protein, carbohydrate and fat can all stimulate body protein accretion in immature animals and in children but the mechanisms by which they do so, and the energy expenditures involved, seem to be different.
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  • 4
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    Empirical economics 24 (1999), S. 23-44 
    ISSN: 1435-8921
    Keywords: Key words: Cointegration ; convergence ; growth ; Kalman filter ; JEL classifications: C22 ; O47 ; O57
    Source: Springer Online Journal Archives 1860-2000
    Topics: Economics
    Notes: Abstract. Augmented Dickey Fuller (ADF) and Kalman filter convergence tests are applied to annual GDPs per head to 16 industrialised countries from 1890 to 1989. Results favour convergence towards the US with a structural break following the Second World War. Estimates suggest that steady-states were higher after the war and that speeds of convergence are different across countries. The Kalman filter method dismissed the no convergence hypothesis more often than its ADF counterpart. This could explain the apparent contradiction in earlier empirical work on similar data sets (cross-section methods tended to favour convergence while time series methods were unable to dismiss the no convergence hypothesis.)
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  • 5
    ISSN: 1570-7458
    Keywords: Vitis vinifera ; Empoasca vitis ; leafhopper ; photosynthesis ; transpiration ; stomatal conductance ; mesophyll conductance ; growth ; yield ; fruit quality ; starch ; carbohydrate reserves
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The impact of the grape leafhopper,Empoasca vitis, on leaf gas exchange, plant growth, yield, fruit quality and carbohydrate reserves of the grapevines,Vitis vinifera L., was studied. Gas exchange was measured on the discolored (red) and the green parts of infested main leaves and on leaves from uninfested vines. Photosynthesis and mesophyll conductance were severely reduced on main leaves showing leafhopper feeding symptoms. The stomatal conductance of the red leaf section of infested main leaves was lower than on undamaged control leaves. Additionally, the red leaf section of infested main leaves showed lower transpiration rates when compared to the green parts of the same leaves and to undamaged control leaves. Gas exchange processes of lateral leaves were not affected by leafhopper feeding. Leafhopperload on main leaves was correlated to visual damage symptoms. At 71.8 leafhopper-days per leaf up to 40% of the main leaf area of the infested plants was discolored from the borders towards the center. Lateral leaves showed no feeding symptoms. Shoot diameter, pruning weight and carbohydrate reserves in the wood were not affected by leafhoppers. Lateral leaf area growth was significantly stimulated on plants infested by leafhoppers. No decrease in yield and fruit quality with leafhopper-loads up to 71.8 leafhopper-days per leaf were observed.
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  • 6
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    Entomologia experimentalis et applicata 80 (1996), S. 141-144 
    ISSN: 1570-7458
    Keywords: Polygonia c-album ; Nymphalidae ; host-plant selection ; growth ; size
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 7
    ISSN: 1570-7458
    Keywords: amylase inhibitor ; red kidney bean ; hard red winter wheat ; growth ; insects ; beetles ; plant resistance ; stored products ; protease inhibitor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Insect α-amylase inhibiting and/or growth inhibiting activities of proteinaceous inhibitors from red kidney bean (Phaseolus vulgaris) and hard red winter wheat (Triticum aestivum) were examined. The bean inhibitor was most effectivein vitro against α-amylases from the red flour beetle (Tribolium castaneum) and the confused flour beetle (T. confusum), followed by those from the rice weevil (Sitophilus oryzae) and yellow mealworm (Tenebrio molitor). The insect enzymes were from two- to 50-fold more susceptible than human salivary α-amylase. When the inhibitors were added at a 1% level to a wheat flour plus germ diet, the growth of red flour beetle larvae was slowed relative to that of the control group of larvae, with the bean inhibitor being more effective than the wheat inhibitor. Development of both the red flour beetle and flat grain beetle (Cryptolestes pusillus) was delayed by 1% bean inhibitor, but development of the sawtoothed grain beetle (Oryzaephilus surinamensis) and lesser grain borer (Rhyzopertha dominica) was not affected by either the bean or wheat inhibitor at the 1% level. Rice weevil adults fed a diet containing 1% bean or wheat inhibitor exhibited more mortality than weevils fed the control diet. When the wheat amylase inhibitor was combined with a cysteine protease inhibitor, E-64, and fed to red flour beetle larvae, a reduction in the growth rate and an increase in the time required for adult eclosion occurred relative to larvae fed either of the inhibitors separately. The bean inhibitor was just as effective alone as when it was combined with the protease inhibitor. These results demonstrate that plant inhibitors of insect digestive enzymes act as growth inhibitors of insects and possibly as plant defense proteins, and open the way to the use of the genes of these inhibitors for genetically improving the resistance of cereals to storage pests.
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  • 8
    ISSN: 1570-7458
    Keywords: butterfly ; Formicidae ; food ; growth ; mutualism ; myrmecophily ; parasitism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Caterpillars of the lycaenid butterfly Maculinea rebeli Hirschke (Lepidoptera: Lycaenidae) live for 11–23 months as social parasites in Myrmica (Hymenoptera: Formicidae) red ant nests, a trait that is believed to have evolved from mutualistic myrmecophilous ancestry. Although Maculinea rebeli caterpillars harm Myrmica larvae, they simultaneously produce copious secretions which the adult worker ants imbibe, perhaps representing a vestige of the ancestral mutualism. We report the results of laboratory experiments designed to test alternative hypotheses: (i) Maculinea rebeli caterpillars provide a beneficial source of sugar in return for being tended by Myrmicaworkers; (ii) Maculinea rebeli harms its host by stressing the workers by competing for available sugar. Comparisons were made of Myrmica worker fitness after 90–450 days under all possible combinations of three experimental treatments: ± M. rebeli caterpillars, ± sucrose and ± ant brood. Caterpillars always reduced the survival of both ant workers and their larvae, even when sugar was not provided, suggesting that M. rebeli is wholly parasitic on all stages in its host colony. The results also confirmed the importance of sucrose in the diet of Myrmica, and showed that M. rebeli caterpillars which eat ant brood to supplement their normal trophallactic feeding by workers develop more quickly - but have the same survival and pupal weights – as caterpillars that are fed solely by worker ants.
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  • 9
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    Entomologia experimentalis et applicata 79 (1996), S. 335-344 
    ISSN: 1570-7458
    Keywords: alkaloid ; developmental time ; early instars ; growth ; Manduca sexta ; molting duration ; phenolics ; plant-herbivore interactions ; thermal regime
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To understand the mechanisms underlying plant-insect herbivore interactions, it is necessary to examine the simultaneous effects of temperature, food quality and larval age. We examined the simultaneous effects of three allelochemicals (tomatine, rutin and chlorogenic acid) on the performance of first and second instar Manduca sexta larvae under two representative thermal regimes 21 : 10°C and 26 : 15°C for spring and summer, respectively. Thermal regime and allelochemicals interacted to influence the time from egg hatch to ecdysis to the third instar. On average, it took about half as much time to reach the third instar at 26 : 15°C as it did at 21 : 10°C. Separately, tomatine and rutin had a negative effect on developmental time from egg hatch to the third instar, but their simutaneous effects were not additive. Chlorogenic acid significantly reduced the negative effect of tomatine. The magnitude of the allelochemical effect was larger at the cooler thermal regime compared to the warmer regime. For instance, chlorogenic acid by itself had no effect at the 26 : 15°C regime, but at the 21 : 10°C regime it significantly shortened total developmental time. The effect of chlorogenic acid on stadium duration was distinctly different for the two instars. Chlorogenic acid shortened stadium duration of first instar larvae. However, depending on thermal regime and the presence of tomatine, chlorogenic acid had a negative, positive or neutral effect on stadium duration of second instar larvae. Molting duration of second instar larvae was shortened by a half day at the warmer thermal regime but was not affected by the allelochemicals. Final larval weight was influenced by rutin and chlorogenic acid. Caterpillars fed diets containing 20 μmoles of rutin were on average 10% lighter than those fed plain diet, whereas those fed diets containing 20 μmoles of chlorogenic adic were on average 7% heavier. However, the effect of chlorogenic acid depended on thermal regime. Overall, our results indicated that: 1) temperature and food quality can interact to influence insect performance and 2) these effects are influenced by larval age.
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  • 10
    ISSN: 1570-7458
    Keywords: ecdysteroid agonists ; juvenile hormone analogue ; Spodoptera exigua ; growth ; moulting ; metamorphosis ; imaginal discs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Biological activity assays with RH 5849 and RH 5992 indicated that both compounds affected growth and development of last-instar larvae ofSpodoptera exigua (Hübner) (Lepidoptera: Noctuidae) in a dose-dependent manner. Within the first 24 h after treatment by continuously offering leaves dipped in a water solution of ≥50 mg/l RH 5849 and ≥0.5 mg/l RH 5992, symptoms of a prematurely induced larval moult and head capsule apolysis were visible. Intoxicated larvae died shortly afterwards, showing signs of unsuccessful ecdysis. LC50-values of RH 5849 and RH 5992 for fifth-instarS. exigua larvae were 110 and 2.5 mg/l, respectively. Pyriproxyfen alone affected the larval stage and disturbed normal metamorphosis. One supernumerary larval instar occurred occasionally. LC50-value for pyriproxyfen was 1.7 mg/l. Larvae simultaneously treated with RH 5849 or RH 5992 and pyriproxyfen, continued to grow until they attained a size and weight about 2–3 times that of the controls. This growth was accompanied by at least one and sometimes two supernumerary moults. Concerning thein vivo imaginal wing disc growth and development, only in larvae treated with 10 and 50 mg/l RH 5849 or 0.5 mg/l RH 5992, tracheole migration was observed earlier than in the controls. When applying 300 mg/l RH 5849 or 3–7 mg/l RH 5992, the discs remained small and no signs of tracheole migration were observed. In larvae simultaneously treated with RH 5849 or RH 5992 and pyriproxyfen, tracheole migration was not prematurely induced and a pupal cuticle was produced in the discs of larvae, undergoing a supernumerary moult. No clear signs of evagination were observed.
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  • 11
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    Entomologia experimentalis et applicata 80 (1996), S. 443-451 
    ISSN: 1570-7458
    Keywords: beta-carboline alkaloid ; Trichoplusia ni ; harmane ; harmaline ; amitriptyline ; imipramine ; monoamine oxidase inhibitor ; tricyclics ; growth ; feeding behavior
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In this study we investigated the effects of two naturally occurring beta-carboline alkaloids and two synthetic tricyclic antidepressants on the growth and food consumption of fifth instar larvae of the cabbage looper, Trichoplusia ni Hübner (Lepidoptera: Noctuidae). In artificial diets at high concentrations (3,000 ppm), harmane, amitriptyline, and imipramine reduce growth and feeding; harmane reduced feeding consistently at a lower concentration (200 ppm). In animals other than insects, beta-carboline alkaloids inhibit monoamine oxidase (MAO) activity and thus affect rates of disposition of serotonin and other monoamine neurotransmitters. Because brain serotonin levels are associated with variation in rates of carbohydrate and protein intake in insects, the effects of beta-carboline alkaloid ingestion on dietary self-selection behavior were examined. Choosing between diets lacking carbohydrate but containing protein and diets lacking protein but containing carbohydrate, larvae consumed a greater proportion of diet containing protein but lacking carbohydrate in the presence of harmane than in its absence. These results are consistent with beta-carboline alkaloid-mediated persistence of serotonin in the brain due to MAO inhibition. Alternatively, these results could reflect alkaloid-mediated peripheral inhibition of sucrose taste receptors influencing ingestive behaviors. That beta-carboline alkaloid ingestion is associated with changes in feeding behavior is consistent with a possible defensive role for these compounds in plant foliage.
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  • 12
    ISSN: 1570-7458
    Keywords: insect endocrinology ; ecdysone ; juvenile hormone ; reproduction ; growth ; inhibin ; oostatin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The first insect folliculostatic peptide was isolated from vitellogenic ovaries of the mosquitoAedes aegypti. This decapeptide directly inhibits trypsin biosynthesis in the gut, and indirectly ovarian development. The factor was named Trypsin Modulating Oostatic Factor or TMOF by its discoverers. From the fleshfly Neobellieria bullata 2 folliculostatins have been isolated, the hexapeptide Neb-TMOF and the 19-mer Neb-colloostatin. The available data suggest that at least 2 of the 3 folliculostatins originate from matrix (like) proteins present in the ovary, a hitherto unknown source of hormones. Furthermore, one of the folliculostatins (Neb-TMOF) is a potent inhibitor of ecdysone biosynthesis by larval ring glands of fleshflies. The discovery of the dipteran folliculostatins, which do not show any resemblance to inhibins of vertebrates, may significantly contribute to a better understanding of the hormonal control of growth in insects and perhaps, in other animals as well. None of the 3 folliculostatins is blocked at its N- or C-terminus. This, in combination with the pleiotropy of their effects and the narrow species specificity make such peptides prime candidates for, testing their potential in insect pest control by means of molecular biological methods.
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  • 13
    ISSN: 1570-7458
    Keywords: Chrysoperla carnea ; food consumption ; growth ; development ; efficiency of food utilization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Larvae of the common green lacewing,Chrysoperla carnea (Stephens)(Neuroptera: Chrysopidae), were fed either an optimal or a suboptimal number of eggs of the mothAnagasta kuehniella (Zeller) in the first and/or second larval instar, or in all three larval instars. Parameters for the duration, growth, and the efficiency of conversion of ingested food to body substance (ECI) of each instar were established for seven different dietary regimes. Larvae that had a suboptimal food supply in the first instar had a significantly longer developmental time, gained significantly less weight, and had a slightly lower ECI in that instar larvae. Suboptimally-fed second less, but remained only slightly longer in that instar and had a similar ECI to optimally-fed second instar larvae. The developmental time of suboptimally-fed third instar larvae was similar to that of optimally-fed larvae of that stage. Whereas the growth of the former was significantly less than that of larvae optimally fed in that instar, the ECI of the former was significantly higher. Despite the relatively smaller size of larvae fed suboptimally in the first and/or second instar, when such larvae were subsequently supplied with an overabundance of prey eggs, they consumed approximately the same number of eggs during the remainder of their larval life as did larvae whose food supply had not been restricted previously. When larvae were allowed to consume different numbers of eggs in their third instar, their gain in weight and therefore the weights attained by the resulting adults (based on the weights of 3-day-old cocoons) had a highly significant positive correlation with the number of eggs consumed in this instar.
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  • 14
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    Entomologia experimentalis et applicata 67 (1993), S. 135-142 
    ISSN: 1570-7458
    Keywords: nutrition ; utilization ; efficiency ; growth ; metabolism ; energy ; respirometry ; Pieris
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Conventional gravimetry and a combination of gravimetry and respirometry were compared for their precision in measuring respiration and metabolic efficiency of growth of final stadiumPieris brassicae L. (Pieridae, Lepidoptera) caterpillars. This was done both for caterpillars feeding on an artificial diet and for caterpillars feeding on excised leaf material of a host plant,Brassica oleracea L. Gravimetry produced significantly greater variation in the total amount of matter respired and the metabolic efficiency than indirect calorimetry for caterpillars feeding on plant material, while the two methods gave similar results for the caterpillars reared on a meridic artificial diet. Respirometry (indirect calorimetry) revealed that caterpillars feeding on the artificial diet were growing with a higher metabolic efficiency than caterpillars feeding on the host plant. This difference was not revealed by conventional gravimetry. It is argued that metabolic efficiencies as derived from gravimetric budget calculations are subject to a number of random errors that distort precise determination of metabolic efficiencies in studies involving plant food.
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  • 15
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    Entomologia experimentalis et applicata 63 (1992), S. 47-54 
    ISSN: 1570-7458
    Keywords: Azadirachtin ; Manduca sexta ; growth ; feeding efficiency ; nitrogen utilisation ; proteinase ; trypsin ; allelochemicals ; antifeedant ; herbivory
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract When given by injection to tobacco hornworm caterpillars, Manduca sexta, the allelochemical azadirachtin inhibits growth without reducing food intake. The growth reducing effect of azadirachtin is therefore in this case independent of the compound's well-known antifeedant effect. The cause of this reduced rate of growth is an increase in the costs associated with growth. These increased costs are largely a consequence of a decrease in the efficiency of utilisation of dietary nitrogen. This is associated with a drastic reduction in the activity of midgut trypsin. Azadirachtin has no effect on the activity of trypsin in vitro. Thus azadirachtin directly or indirectly inhibits the production of trypsin by the enzyme-secreting cells of the midgut wall; it is suggested that this is the cause of the increased costs and reduced rate of growth. The interesting parallel between this plant defence strategy and that of direct inhibition of herbivore proteinases by allelochemical proteinase inhibitors is discussed.
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  • 16
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    Entomologia experimentalis et applicata 53 (1989), S. 257-265 
    ISSN: 1570-7458
    Keywords: Antifeedant ; insecticide ; bisabolangelone ; analog ; feeding ; growth ; molting ; mortality ; sesquiterpenoid ; development ; phago-stimulant ; Pieris brassicae L. ; Lepidoptera ; Pieridae ; Angelica silvestris L. ; Umbelliferae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Bisabolangelone und drei Analoge wurden unter Laboratoriumsbedingungen auf ihre frasshemmende Wirkung in Raupen von Pieris brassicae L. (Lepidoptera: Pieridae) geprüft. Die Resultate von Zweifach-Wahlversuchen zeigten, dass sowohl die frasshemmende als auch die insektizide Wirkung von Bisabolangelone in den Analogen stark reduziert ist oder ganz verloren ging. Weitere Untersuchungen mit niedrigen Bisabolangelone-Dosen zeigten, dass das Fehlen von wohlschmeckender Nahrung zu einer drastischen Reduktion der Nahrungsaufnahme und des Wachstums der Raupen sowie zur Verhinderung der Häutung und zu hoher Mortalität führt. Deformationen, wie sie von bisabolangelone-behandelten Vorratsschädlingen gemeldet wurden, konnten bei P. brassicae nicht beobachtet werden.
    Notes: Abstract Bisabolangelone and three analogs were assayed, under laboratory conditions, for their antifeedant activity against Pieris brassicae L. (Lepidoptera: Pieridae) larvae. The results of dual-choice studies revealed that the potent antifeedant activity of bisabolangelone is greatly reduced or lost in the analogs. Insecticidal activity observed in the former was not detected either. Further studies using the lower rates of bisabolangelone showed that the absence of a preferable food resulted in a drastic reduction in feeding and growth, and produced high mortality and inhibition of molting. No developmental deformities reported in stored product insects treated with bisabolangelone were observed in P. brassicae during our studies.
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  • 17
    ISSN: 1572-879X
    Keywords: aluminum oxide ; hydroxyl groups ; rhodium ; growth ; scanning tunneling microscopy ; infrared spectroscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract In order to investigate how the presence of surface hydroxyl groups on oxide surfaces affects the interaction with the supported metal, we have modified a well-ordered alumina film on NiAl(110) by Al deposition and subsequent exposure to water. This procedure yields a hydroxylated alumina surface as revealed by infrared and high-resolution electron energy loss spectroscopy. By means of scanning tunneling microscopy, we have studied the growth of rhodium on the modified film at 300 K. Clear differences in the particle distribution and density are observed in comparison to the clean substrate. While, in the latter case, decoration of domain boundaries as typical defects of the oxide film governs the growth mode, a more isotropic island distribution and a drastically increased particle density is found on the hydroxylated surface. From infrared data, it can be deduced that the growth is connected with the consumption of the hydroxyl groups due to the interaction between the metal deposit and the hydroxylated areas. This finding is in line with photoemission results published earlier.
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  • 18
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    Acta applicandae mathematicae 9 (1987), S. 219-237 
    ISSN: 1572-9036
    Keywords: 34A34 ; 34D99 ; 90A16 ; Nonlinear differential equations ; stability ; growth ; economic dynamics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract This paper analyses the implications of persistent growth upon the stability properties of dynamic models. Besides the traditional concept of asymptotic stability, new stability criteria-strong/weak absolute, strong/weak relative, strong/weak logarithmic stability-are introduced, and global stability conditions for satisfying these criteria are stated for general first-order autonomous differential equations. The conflict between rapidity of growth and the degree of stability is demonstrated. Economic applications of the stability theorems are illustrated within the growth models of Harrod and Solow.
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  • 19
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    Cellular and molecular life sciences 41 (1985), S. 1554-1557 
    ISSN: 1420-9071
    Keywords: Immunocytochemistry ; neuropeptide Y ; radioimmunoassay ; rat pancreas
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Significant quantities of a newly discovered peptide, neuropeptide Y, were found in the rat pancreas, where they were localized to nerves in the exocrine parenchyma and around arterial and ductal structures. Although unaffected by surgical parasympathectomy, the periarterial and periductal nerves were abolished by chemical sympathectomy, suggesting that NPY is partially costored with sympathetic transmitters in nerve fibers.
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    Cellular and molecular life sciences 41 (1985), S. 1621-1621 
    ISSN: 1420-9071
    Keywords: Chlorella ; cadmium ; growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Growth of 14 strains from fiveChlorella species is rather insensitive towards cadmium. One strain (211-1a) ofC. saccharophila, however, was found to have a sensitivity towards this toxic heavy metal about 100 times higher than that of the other strains ofC. saccharophila.
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  • 21
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    Cellular and molecular life sciences 42 (1986), S. 1170-1173 
    ISSN: 1420-9071
    Keywords: Lampreys ; Geotria australis ; ammocoetes ; larval life ; growth ; metamorphosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The average duration of larval life in the anadromous lamprey,Geotria australis (the sole representative of the Geotriidae) is estimated as 41/4 years. Compared with other lampreys, the ammocoetes ofG. australis have a slow growth rate, increase in length during the year preceding metamorphosis and typically enter metamorphosis at a small mean length (〈100 mm) and weight (〈1.2 g).
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  • 22
    ISSN: 1570-7458
    Keywords: Alsophila pometaria ; Geometridae ; Anisota senatoria ; Citheroniidae ; Quercus ; nutritional ecology ; herbivory ; nutritional indices ; consumption ; growth ; utilization efficiency ; nitrogen ; water ; tannins ; phenols ; gut pH ; digestive enzymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Alimentées sur feuillage jeune de chêne, les chenilles d'Alsophila pometaria avaient un taux relatif de croissance (RGR) et un taux relatif d'accumulation d'azote (RNAR) plus élevés que les chenilles d'Anisota senatoria alimentées sur feuillage mûr de chêne. Bien que le jeune feuillage soit plus efficacement digéré par A. pometaria (AD plus élevé), il n'est pas assimilé et utilisé pour la croissance avec de meilleurs rendements (les ECI ne sont pas différents). Ainsi le taux de croissance plus élevé d'A. pometaria est dû entièrement à un taux de consommation plus important (RCR et RNCR). Le feuillage jeune est significativement plus riche en azote et en eau que le feuillage mûr, mais les niveaux de phénol et de tanins sont les mêmes. A pometaria consomme les feuilles de différentes espèces de chênes au même taux, indépendamment de la teneur en azote, tandis que A. senatoria accroît sa consommation en réponse à une diminution de la teneur en azote. Il en résulte que le taux de croissance d'A. pometaria dépend directement de la teneur en azote des feuilles, tandis que celui d'A. senatoria en est indépendant. Les systèmes digestifs des deux insectes sont biochimiquement semblables et sont efficaces pour la digestion des protéines. Les tanins et les phénols n'influent pas sur les indices nutritionnels de ces deux espèces. Nous estimons que le principal intérêt de l'alimentation printanière est la disponibilité en feuillage succulent, riche en azote, et non l'absence de feuilles à haute teneur en tanin. L'alimentation printanière semble correspondre à une strategie alimentaire qui favorise la croissance aux dépens de l'efficacité tandis que l'alimentation en fin d'été est une stratégie qui favorise l'efficacité sur la rapidité.
    Notes: Abstract The larvae of Alsophila pometaria (Harr.), feeding on the young foliage of oak, has a higher relative growth rate (RGR) and relative nitrogen accumulation rate (RNAR) than the larvae of Anisota senatoria (J. E. Smith), feeding on the mature foliage of oak. Although the young oak foliage is more efficiently digested by A. pometaria (higher AD's), it is not more efficiently assimilated and used for growth (no difference in ECI's). Thus, the higher growth rate of A. pometaria is due entirely to a higher consumption rate (RCR and RNCR). Young foliage is significantly higher in nitrogen and water than mature foliage, but phenol and tannin levels are comparable in young and old foliage. A. pometaria consumes the foliage of different oak species at the same rate, independent of nitrogen content, while A. senatoria increases its consumption rate in response to decreased nitrogen levels. As a result, the growth rate of A. pometaria is directly related to leaf nitrogen content, while the growth rate of A. senatoria is independent of leaf nitrogen. The two species of insects have digestive systems that are very similar biochemically, and that are well-designed for effective protein digestion. Tannins and phenols do not influence the nutrional indices of either species. We suggest that the major benefit of spring feeding is the availability of succulent, high-nitrogen foliage, and not the avoidance of high-tannin foliage. The spring feeder appears to have a feeding strategy that favors rapid growth at the expense of efficiency, while the late summer feeder has a strategy that favors efficiency over rate.
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  • 23
    ISSN: 1570-7458
    Keywords: Homoptera ; Aphididae ; Acyrthosiphon pisum ; Medicago sativa ; resistance ; amino acids ; sucrose ; phloem sap ; artificial diet ; ingestion ; growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Sur des milieux holidiques se différenciant par leur concentration en acides aminés ou en saccharose ou par leur spectre en acides aminés et copiant la sève de deux variétés de luzerne (sensible et résistante) nous avons étudié différents paramètres biologiques d'un biotype d'Acyrthosiphon pisum Harris afin de mieux comprendre les mécanismes de résistance à ce puceron. Que ce soit pour le reproduction et la survie des adultes ou pour la croissance et le développment larvaire, nous montrons l'influence spécifique de la concentration en saccharose et en acides aminés ainsi que celle du spectre en acides aminés des régimes artificiels. Ces différences de performances de A. pisum peuvent en partie être expliquées par les différences d'ingestion. Néanmoins pour des rapport saccharose/acides aminés identiques et pour des niveaux d'ingestion égaux, il existe selon les milieux de grandes différences de taux de reproduction et donc d'efficacités métaboliques. Les milieux copiant la sève de variétés sensible et résistante permettent jusqu'à présent d'expliquer une partie des observations faites sur végétal, mais démontrent que l'ignorance de certains facteurs phagostimulants présents dans la plante empêche une interprétation complète des effets observés.
    Notes: Abstract In order to understand the mechanisms of resistance of two lucerne cultivars (susceptible ‘Resistador’ and resistant ‘Lahontan’ clones) to a French biotype of Acyrthosiphon pisum Harris, several biological parameters of this aphid were investigated on sap-copied holidic diets differing either by their amino acid or sucrose concentrations or by the relative proportions of some amino acids. We recorded the quantitative influence of nutrient levels and amino acid profiles on adult survival and reproduction, as well as on larval growth and development. The differences in A. pisum performances on artificial diets may partly by explained by variable ingestion rates, but also by differences in metabolic efficiency of the ingested nutrients, which greatly depends on amino acid composition. Although holidic diet experiments partially confirm the biological assays of resistance on plants, they also point out a lack in our understanding of the factors present in the sap which are involved in phagostimulation in vivo, thus preventing a complete interpretation of observed resistance.
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    BioMetals 5 (1992), S. 149-156 
    ISSN: 1572-8773
    Keywords: Hg2+ toxicity ; cyanobacterium ; Nostoc calcicola ; growth ; photopigments ; nucleic acids ; photosynthesis ; membrane integrity ; nutrient uptake ; enzymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Toxicological responses of the filamentous N2-fixing cyanobacteriumNostoc calcicola Bréb. towards Hg2+ were studied to enumerate the decisive lethal events. In low-dose, long-term experiments (0.05–0.25 μm Hg2+, 10 days), photoautotrophic growth was severely inhibited with concurrent loss of photosynthetic pigments (phycocyanin〉chlorophyll α〉carotenoids) and nucleic acids. The termination of growth after a day 4 exposure to 0.25 μm Hg2+ has been attributed to the complete inhibition ofin vivo photosynthetic activity in the cyanobacterium (O2 evolution〉14CO2 incorporation). The elevated Hg2+ concentrations irreversibly damaged the cell membrance as observed under light microscopy, and as indicated by the leakage of intracellular electrolytes and phycocyanin. In high-dose, short-term experiments (0.5–20.0 μm Hg2+, up to 6 h), thein vivo activities of selected enzymes (glutamine synthetase 〉 nitrate reductase 〉 nitrogenase) were less inhibited by Hg2+ than the uptake of nutrient ions (NH 4 + 〉NO 3 − 〉PO 4 3− ).
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    Entomologia experimentalis et applicata 35 (1984), S. 75-81 
    ISSN: 1570-7458
    Keywords: Nezara viridula ; insect dietetics ; growth ; fecundity ; flight capacity ; Heteroptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Les effets du régime alimentaire pendant le 5ème stade sur les performances imaginales de N. viridula ont été déterminés en comparant la croissance, la fécondité, la durée du vol captif, et l'accumulation de corps gras chez des insectes élevés sur 4 régimes stérilisés. A partir de la formation des larves de 5ème stade, elles ont été alimentées sur haricots verts et arachides, ou transférées sur un régime formé exclusivement d'arachides, ou de haricots verts, ou de graines de soja ‘trempées’, cependant la durée du 5ème stade. Les insectes ont été transférés sur un régime de haricots verts et arachides à leur émergence. Les femelles élevées continuellement de haricots verts et arachides étaient plus lourdes (P〈0,01) à l'émergence (161,6 mg) que celles élevées sur haricots verts (146,3 mg) pendant le 5ème stade, et pondent plus d'oeufs (P〈0,08) pendant une durée de 34 jours (100,9 oeufs) que celles élevées sur arachides (54,7 oeufs). Plus de femelles élevées sur haricots verts et arachides (76,2%) pondent que celles élevées sur arachides (61,1%), soja ‘trempé’ (62,5%), ou haricots verts (60,9%). Les femelles élevées sur arachides volaient plus longtemps (P 〈 0,01) (42,9 mn) que celles élevées sur haricots verts (19,8 mn) pendant des vols captifs le 3ème jour après l'émergence. 78% de toutes les femelles élevées sur arachides volaient 〉-30 mn, contre 50% pour celles élevées sur haricots verts et arachides, ou soja ‘trempé’, et 35% pour celles élevées sur haricots verts. Seulement 7% des femelles élevées sur haricots verts volaient à la 60ème minute à la fin du test, contre 25% pour les femelles soja ‘trempé’, 33% pour les femelles haricots verts et arachides, et 47% pour celles élevées sur arachides. Les femelles ont été disséquées 34 jours après l'émergence pour déterminer l'importance du corps gras. Les indices de corps gras (Kiritani, 1963) montraient que les femelles élevées sur arachides (1,8) et graines de soja (1,7) ont apparemment un corps gras plus développé que celles élevées sur haricots verts et arachides (1,5), our haricots verts (1,2), pendant le 5ème stade. Ces résultats montrent que l'alimentation pendant le dernier stade larvaire de N. viridula affecte irréversiblement la croissance, la fécondité et l'aptitude au vol des femelles. En estimant que des résultats semblables auraient été obtenus avec des insectes élevés sur ces 4 aliments depuis l'éclosion, haricots verts et arachides constituent le meilleur aliment pour l'élevage de N. viridula. Ce régime fournit une graine mûre, vraisemblablement nécessaire pour l'accumulation d'un corps gras suffisant et une aptitude au vol migratoire, et une plante verte qui fournit les aliments apparement essentiels à l'ovogenèse.
    Notes: Abstract The effects of diet during the fifth stadium on performance of adult N. viridula were determined through comparing growth, fecundity, and duration of tethered flight of insects reared on four axenic diets: fresh green bean, raw-shelled peanut, a combination of the two and soaked soybean seed. Females reared on the combination diet were heavier than those reared on green beans and oviposited more than those reared on peanuts. Three-day-old adult females reared on peanuts flew longer than those reared on green beans. More females reared on diets containing mature seed showed a capacity for extended flight and had larger fat bodies at 34-days postemergence. Thus, inadequate diet during the fifth stadium irreversibly affected growth, development, reproduction and flight capacity.
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    Entomologia experimentalis et applicata 41 (1986), S. 121-133 
    ISSN: 1570-7458
    Keywords: Ips typographus ; bark beetle ; Scolytidae ; intra-specific larval competition ; growth ; simulation model ; breeding pattern
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Die Konkurrenz zwischen Larven des Borkenkäfers Ips typographus L. in Rundhölzern von Picea abies Karsten wurde im Laboratorium studiert. Um die Annahme zu testen, dass die Mortalität bei Borkenkäferlarven hauptsächlich von Futterkonkurrenz stammt, wurden die Parameter dieses Vorgangs geschätzt, die Zahl der Überlebenden vorausgesagt und verglichen mit der tatsächlichen Zahl Überlebender in jedem Rundholz. Die erforderlichen Voraussagen wurden mit dem Simulationsmodell von De Jong & Saarenmaa (1985) gemacht. In diesem Modell wird angenommen, dass die Larven Futter aufnehmen, in dem sie sich in der zweidimensionalen Rindenschicht bewegen und dass sie sterben oder sich vorzeitig in untergewichtige Käfer verwandeln, wenn sie in ein Gebiet gelangen, dessen Futter erschöpft ist. Es wurde geschlossen, dass der wichtigste Aspekt der Larvenbewegung im Hinblick aufs Überleben das gegenseitige Ausweichen ist. Die Muster der Larvengänge, wie sie aus einem Muttergang aufgrund gegenseitigen Ausweichens entstehen, wurden berechnet; sie stimmten sehr gut überein mit den beobachteten Mustern. Das Wachstum und das Überleben wurden in separaten Versuchen untersucht. Es wurde gezeigt, dass vorzeitiger Entwicklungsabschluss als Folge von Nahrungsknappheit eintritt. Das Überleben hängt davon ab, ob die Larve genug Futter zum Verpuppen erhalten hat. Dass die Larve eine bestimmte Grösse erreicht (Wachstum), war proportional zur Bewegung über eine bestimmte Distanz in der Rindenschicht. Unter den Versuchsbedingungen nahm das potentielle Trockengewicht der Puppen um 0,08 mg zu mit jedem mm Larvengang. Die minimale zur Verpuppung erforderliche Futtermenge war 20 mm Ganglänge, was proportional zu 1,6 mg Puppentrockengewicht ist. Aus diesen Resultaten wird geschlossen, dass Futterkonkurrenz die hauptsächliche Mortalitätsursache unter Larven von I. typographus in Rundhölzern im Laboratorium ist. Zudem wurde das Simulationsmodell erfolgreich überprüft; deshalb scheint es, dass Futterkonkurrenz entsprechend der vorigen Hypothese erfolgt. Die Annahme scheint vertretbar, dass dieser letzte Schluss auch für I. typographus unter natürlicheren Bedingungen zutrifft.
    Notes: Abstract A series of laboratory experiments were conducted to provide parameters for a simulation model for Ips typographus L. (Coleoptera, Scolytidae). This model illustrates larval competition and predicts brood survival in bolts. Brood survival depended upon larvae attaining a weight of at least 1.6 mg dry weight. The pattern of larval foraging in a bolt is hypothesized to be regulated by mutual avoidance among larvae. An analytical solution is presented for the construction of the pattern of larval paths originating from a single maternal gallery.
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  • 27
    ISSN: 1570-7458
    Keywords: Syrphidae ; Metasyrphus corollae ; cereal aphid ; Sitobion avenae ; quantification ; field populations ; biomass ; voracity ; growth ; development ; kill rate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Le taux de mortalité de S. avenae provoqué par les larves de M. corollae a été mesuré au laboratoire pour évaluer l'impact de ce prédateur sur les populations de ce puceron dans la nature. L'évolution du poids des larves et de la biomasse de pucerons tués a été suivie tous les jours dans des conditions de température et humidité constantes. Un taux moyen de mortalité a été déterminé pour chaque stade larvaire du prédateur, il a servi à déduire les niveaux de mortalité quotidienne dans les champs.
    Notes: Abstract The rate of kill of cereal aphids by larvae of Metasyrphus corollae (F.) (Diptera: Syrphidae) was measured in the laboratory with the aim of assessing the impact of this predator on field populations of cereal aphids. Daily measurements were taken of changes in both the larval weight and the biomass of aphids killed by M. corollae in conditions of constant temperature and humidity. A mean relative kill rate was defined and calculated for each larval instar of the predator from which estimates of field daily rates of kill can be made.
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  • 28
    ISSN: 1570-7458
    Keywords: Coccinellidae ; Harmonia axyridis ; Semiadalia undecimnotata ; Aphids ; Acyrthosiphon pisum ; Pyralidae ; Anagasta kuehniella ; growth ; reproduction ; breeding ; natural prey ; unnatural prey
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Summary Aphidophagous coccinellids Harmonia axyridis and Semiadalia undecimnotata were reared on eggs of the pyralid moth, Anagasta kuehniella during three generations previously killed by exposure to ultra violet radiation. Incidentally, these eggs are used for the mass production of Trichogramma. For H. axyridis larvae, this prey has the same nutritional value as the aphid Acyrthosiphon pisum (control). In adults, it causes an increase in reproductive activity which persisted during three generations. Decreased egg fertility is compensated for by stimulated oviposition. However, regardless of generations, the number of larvae produced per female per day remained equal to or became larger than the control. When the adults are transferred to aphid prey after 15 days of oviposition on the substitution food, a high level of fecundity is maintained and egg fertility improved, particulary during the last two generations. In S. undecimnotata, a specific aphid predator, preying on substitute prey is associated with prolonged pre-imaginal development and increased mortality, the latter affecting up to 50% of the larvae during the third generation. In adults, it induces a deterioration of the reproductive capacities (fecundity, fertility) which however seemed to improve during the third generation. Substitute prey has negative effect on these adults. At present, only H. axyridis might be considered for temporary mass production, as suggested by the results obtained at the end of the first generation.
    Notes: Résumé Les coccinelles aphidiphages Harmonia axyridis Pallas et Semiadalia undecimnotata Schneider (Coccinellidae) ont été élevées pendant trois générations à l'aide d'oeufs d'Anagasta kuehniella Zell. (Pyralidae) tués par une exposition à un rayonnement ultraviolet. Chez la première espèce, cette proie de substitution présente une valuer alimentaire comparable si ce n'est pas supérieure à celle du puceron Acyrthosiphon pisum (Harris). Ces résultats permettent d'envisager, dès à présent, une production massive dans ces conditions trophiques. La mise en place d'un élevage continu dépend de l'évolution sur un grand nombre de générations de certains paramètres biologiques notamment de la fertilité des femelles. Chez la seconde espèce, une telle application paraît impossible pour l'instant.
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    Entomologia experimentalis et applicata 29 (1981), S. 109-116 
    ISSN: 1570-7458
    Keywords: Heteronychus arator ; Scarabaeidae ; larvae ; consumption ; utilization ; growth ; feeding ; roots ; Paspalum dilatatum ; Lolium perenne ; Trifolium repens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Die Futteraufnahme, die Futterverwertung und das Wachstum von Drittstadiumlarven von H. arator beim Fressen von Wurzeln von Paspalum dilatatum, Raygras (Lolium perenne) und Weissklee (Trifolium repens) wurden gravimetrisch gemessen. Der durchschnittliche Futterverzehrindex auf Gräsern war 0.65 mg/mg Larve/Woche verglichen mit 0.18 auf Weissklee. Die relative Wachstumsrate auf Paspalum und Raygras was 0.158 und 0.124 mg/mg Larve/Woche verglichen mit 0.043 auf Weissklee, was den geringen Verzehr an Weisskleewurzel widerspiegelt. Die Verwertung der gefressenen Wurzel war 22%, 18% und 24% für Paspalum, Ryagras und Weissklee. Das Fehlen gesicherter Unterschiede in der Futterverwertung der drei Pflanzenarten deutet darauf hin, dass die geringe Wachstumsrate auf Weissklee bloss durch geringe Futteraufnahme verursacht ist. Es wurde gezeigt, dass in Weisskleewurzel Frasinhibitoren für H. arator vorhanden sind. Die erhaltenen Schätzungen für Futteraufnahme durch Scarabaeiden sind die besten Daten für wurzelfressende Arten und liefern eine Basis, um den Einfluss der Weidepflanzen auf H. arator Populationen zu verstehen.
    Notes: Abstract The consumotion, utilization and growth of third-instar larvae of black beetle, Heteronychus arator (F.) feeding on roots of ryegrass (Lolium perenne), paspalum (Paspalum dilatatum) and white clover (Trifolium repens) were studied. Consumption was greater on the grasses than on white clover when assessed by gravimetric methods. Larvae had a higher growth rate on the grasses. Utilization of the roots of the three species was similar indicating that poor growth on white clover is the result of low consumption probably induced by the presence of feeding deterrents in the roots.
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    Entomologia experimentalis et applicata 48 (1988), S. 225-232 
    ISSN: 1570-7458
    Keywords: Avena macrostachya ; Hordeum bogdani ; resistance ; Rhopalosiphum padi ; growth ; excretion ; amino acids ; leaf anatomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumè Lors d'examens systématiques antérieurs, Avena macrostachya (Bal. ex Coss. & Dur.) et Hordeum bogdani (Wil.) ont présenté une résistance élevée au puceron Rhopalosiphum padi (L.). Lorsqu'ils avaient un choix comprenant de l'avoine et de l'orge cultivés, les pucerons ailés ont atterri en nombres moins importants sur les espèces sauvages. Les résultats étaient cependant variables dans le complexe avoine. La production de nymphes et le poids des adultes étaient plus élevés sur espèces cultivées, ainsi que la durée du développement était plus longue sur les espèces sauvages. A partir du troisième stade, l'excrétion de miellat a été significativement plus faible sur les espèces résistantes. En général, le miellat y contenait moins de 1% d'acides aminés bien que sur H. vulgare il en contînt 3,5%. Les pourcentages d'acides aminés libres du miellat étaient semblables sur toutes les plantes (5,2–7,6%), à l'exception de H. vulgare sur lequel les pucerons excrétaient 22% des taux ingérés. Les acides aminés excrétés en fortes quantités sur les différentes plantes, comprenaient l'asparagine, l'acide γ-aminobutyrique, l'acide glutamique et la glycine. Des coupes de tissus n'ont révélé aucun obstacle mécanique clair à la pénétration des stylets. Les possibilités d'utiliser ces espèces sauvages comme source de résistance aux pucerons dans la sélection de l'avoine et de l'orge ont été examinées.
    Notes: Abstract In previous screening tests the two wild crop relatives Avena macrostachya (Bal., ex Coss. et Dur.) and Hordeum bogdani (Wil.) demonstrated a high degree of resistance to the aphid Rhopalosiphum padi (L.). In a choice situation using wild and cultivated oats and barley, alate aphids settled in lower numbers on the wild species. The results were, however, variable in the Avena combination. Nymph production was significantly higher, development time shorter and adult weight higher on the cultivated varieties. From the third instar and onwards the excretion of honeydew was significantly lower on the resistant plants. In general the honeydew contained less than 1% free amino acids although excreta from H. vulgare contained 3.5%. The percentage of free amino acids found in the honeydew was similar for all plant species (5.2–7.6%) except for H. vulgare, on which the aphids excreted 22% of the amounts ingested. Amino acids excreted in high proportions on all plants included asparagine, γ-aminobutyric acid, glutamic acid, and glycine. Tissue sectioning did not reveal any obvious mechanical barriers to stylet penetration. The potential use of these wild species as sources for aphid resistance breeding in oats and barley is considered.
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    Entomologia experimentalis et applicata 61 (1991), S. 59-72 
    ISSN: 1570-7458
    Keywords: Consumption ; food utilization efficiencies ; growth ; Lycopersicon esculentum ; Manduca sexta ; Nicotiana tabacum ; potassium ; tobacco hornworm ; water regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Third instar tobacco hornworms (Manduca sexta L.: Sphingidae) on low dietary potassium had a lower relative growth rate than individuals on diets with potassium concentrations reflecting those in host-plants, due to decreased consumption rate, lower efficiencies of conversion of ingested and digested food (ECI and ECD), and a prolonged growth/feeding phase. Furthermore, these larvae, when placed on a diet with a moderate potassium concentration through the fourth stadium, ended up being smaller due to lower ECI and less biomass gained, and had a prolonged growth phase, which suggest an irreversible cost of the previous low potassium diet. Third instar hornworms on high potassium diets had lower ECI and ECD, and they had a prolonged growth phase. These individuals, when placed on a moderate potassium diet in the fourth stadium, gained less biomass, than those previously offered hostplant-like-potassium diets. Body potassium concentrations (% dw) at the end of the third stadium were similar among treatment groups. With increasing potassium concentrations in the diet, utilization efficiencies of potassium decreased and potassium concentrations in the frass increased. Correspondingly, water content (% fw) of the newly-molted fourth instar larvae declined with increasing potassium, indicating a passive loss of water during potassium excretion. Low and high dietary potassium reduced survivorship of third instar larvae; fourth instar caterpillars previously fed the low potassium diet also had poor survivorship. We conclude that, within the normal range of potassium concentrations in the hostplants, caterpillar performance is largely unaffected by potassium concentration, but that potassium-poor and potassium-rich diets, such as those hornworms may sometimes experience, can reduce growth and survivorship.
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    Calcified tissue international 12 (1973), S. 313-321 
    ISSN: 1432-0827
    Keywords: Cathepsin D ; Extracellular ; Ossification ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé De la cathepsine D extracellulaire d'origine principalement ostéoblastique a été démontrée par immunohistochimie dans le cartilage en voide de calcification de cultures d'os des membres d'embryons de poulet. L'intérêt de ce fait pour l'ossification est discuté.
    Abstract: Zusammenfassung Es wurde mit einer immunohistochemischen Methode gezeigt, daß extrazelluläres Kathepsin D, welches hauptsächlich aus Osteoblasten gewonnen wurde, Knorpel von kultivierten Knochen von Kükenembryonen mineralisiert. Die Bedeutung dieser Feststellung für den Mechanismus der Knochenbildung wird diskutiert.
    Notes: Abstract Extracellular cathepsin D derived mainly from osteoblasts has been demonstrated immunohistochemically in ossifying cartilage of cultured embryonic chick limb bones. The relevance of this observation to the mechanism of ossification is discussed.
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  • 33
    ISSN: 1432-1351
    Keywords: Photoreception ; Extraretinal Photoreceptor ; Chromophore ; Opsin ; Reptile ; Immunocytochemistry ; HPLC
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Since the beginning of this century evidence has accumulated which demonstrates that non-mammalian vertebrates possess photoreceptors situated deep within the brain. While many attempts have been made to localize these sensory cells, studies have either failed or been inconclusive. In this report we have used several experimental approaches to localize the deep brain photoreceptors of the lizard Anolis carolinensis. Using 3 antibodies that bind vertebrate cone opsins, we have immunolabelled cerebrospinal fluid (CSF)-contacting neurons located at the ventricular border within the nucleus ventromedialis of the septum. Western blot analysis indicates that these antibodies recognized a single 40 kD protein in ocular, anterior brain, and pineal extracts. Immunoblots of rodent brain did not show a similar protein band. We have also identified specific retinoids associated with phototransduction (11-cis and all-trans-3,4-didehydroretinaldehyde) within anterior brain extracts. This combined data provides the most detailed analysis of deep brain photoreceptors in any vertebrate. Consequently, we feel Anolis provides an excellent model to study this unexplored sensory system of the vertebrates.
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  • 34
    ISSN: 1432-1351
    Keywords: Egg laying hormone ; Aplysia ; Binding protein ; Immunocytochemistry ; Reproductive system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A protein solubilized from a membrane preparation of the gonad of Aplysia californica has been isolated by affinity chromatography, using bag cell egg-laying hormone (ELH) as the bound ligand, and partially purified and characterized by gel electrophoresis. The protein has an apparent molecular weight of 52 kDa and consists of two disulfide-linked subunits of about 30 kDa each. The protein is glycosylated and has an acidic pI. Approximately 10–15 μg of this protein can be isolated from a single ovotestis, representing less than 1% of the total protein in the gonad; but the protein could not be detected in buccal mass or body wall, tissues which do not have apparent response to ELH. Antibodies generated against this ELH-binding protein (ELHBP) were used to localize sites in the ovotestis which might contain this molecule and thus represent targets for egg-laying hormone. Immunocytochemical results indicate that the oocytes are a rich source of this protein, since their cytoplasm was the only detectable site of immunoreactivity. Whether this binding protein represents an egg-laying hormone receptor is uncertain, but its prevalence in oocytes suggests that ELH plays a signaling role on these gametes.
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  • 35
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    European journal of nutrition 36 (1997), S. 205-213 
    ISSN: 1436-6215
    Keywords: Frying ; growth ; liver lipids ; lipaemia ; olive oil ; pregnancy ; rat ; Fritierung ; Wachstum ; Leberfett ; Olivenöl ; Schwangerschaft ; Ratte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Zusammenfassung Um Informartionen über den Zusammenhang zwischen der Aufnahme von Fett, das zum Fritieren benutzt wurde, und dem Fetthaushalt während Perioden starken Körperaufbaus zu haben, wurde der Einfluß der Aufnahme von frischem Olivenöl (Gehalt polarer Verbindungen, 2 %; Ölsäure 78,9 mg/100 mg Öl, und Linolsäure 7 mg/100 mg Öl) und von Olivenöl, das 15 mal in Folge für das Fritieren von Kartoffeln benutzt worden war (Gehalt polarer Verbindungen 9 %; Ölsäure 75,8 mg/100mg Öl und Linolsäure 6,2 mg/100mg Öl) während der Gravidität, untersucht. Dazu wurden trächtige Wistar Ratten in zwei Gruppen geteilt, die beide eine isokalorische Diät bekamen, deren Fettanteil 15 % von frischem (unbenutztem) (P1) bzw. fritiertem (benutztem) (P2) Olivenöl stammte mit nicht trächtigen Ratten verglichen. Die Gravidität erhöhte (p〈0,01) die Futteraufnahme, das Körpergewicht, die Gewichtszunahmen und die Futterverwertung. Die Ölqualität beeinflußte dagegen diese Parameter nicht. Während der Gravidität stiegen die Serumwerte der Triglyceride (TG) (p〈0,01) und des Cholesterins (TC) (p〈0,05) an, während die der Phosphatide (PH) sanken (p〈0,01). Ein signifikanter Effekt der Ölqualität und eine Wechselwirkung zwischen Gravidität und Öl wurde für TG und PH festgestellt. Das Gewicht und der Fettgehalt der Leber der trächtigen Ratten stiegen signifikant an (p.〈0.05), Leber TC, TG und PH stiegen während der Gravidität (ungefähr um das 3-fache der Ausgangswerte), aber es traten keine signifikanten Unterschiede zwischen der Aufnahme von benutztem und nicht benutztem Öl (P2 vs P1) auf. Die Ergebnisse zeigen, daß die Aufnahme von leicht verdorbenem Olivenöl als alleinige Fettquelle der Nahrung keine besonderen Folgen für die Gravidität hat, was die Gewichtszunahme der Mütter und der Feten, die Lipämie und die Zusammensetzung des Leberfetts betrifft.
    Notes: Summary The effect of the consumption of unused olive oil (polar content, 2 %; oleic acid, 78.9 mg/100 mg oil, and linoleic acid 7 mg/100 mg oil) and olive oil used discontinuously for frying potatoes 15 times (polar content, 9 %; oleic acid, 75.8 mg/100 mg oil and linoleic acid 6.2 mg/100 mg oil) was studied in pregnant rats with the aim of better understanding the relationship between the consumption of fat used in frying and lipid metabolism during periods of intense anabolism. Trials were performed in pregnant Wistar rats, divided into 2 groups and fed isocaloric diets in which the fat content (15 % wt/wt) consisted of unused olive oil (P1) or oil previously used for frying (P2), and the results were compared with those of nonpregnant rats fed unused olive oil (NP1) and olive oil used for frying (NP2). Pregnancy increased (p〈0.01) food intake, body weight, weight gain, and food efficiency ratio (P2 vs NP2 and P1 vs NP1, respectively), but the treatment of oil included in the diets did not alter these parameters. Gestation significantly increased the serum triglyceride (TG) (p〈0.01) and total cholesterol (TC) (p〈0.05) concentrations and diminished that of phospholipids (PH) (p〈0.01). A significant effect of the type of oil consumed and a pregnancy x oil interaction on Tg and PH levels was observed. The weight of the liver and its fat content increased significantly (p〈0.05) as a result of pregnancy. Liver TC, TG, and PH increased (approximately 3 times the original values) during gestation, but no significant differences due to the intake of used or unused oil (P2 vs P1) were observed. The results indicate that the consumption of moderately altered olive oil, as the sole source of fat, does not alter the effect of pregnancy on the mothers' weight gain, lipaemia, and hepatic fat composition to any important degree.
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  • 36
    ISSN: 1436-6215
    Keywords: Body composition ; fat ; growth ; somatotropin ; pig ; Körperzusammensetzung ; Fett ; Wachstum ; Somatotropin -Schwein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Zusammenfassung An 78 männlichen Kastraten und weiblichen Schweinen der Kreuzung Pietrain x (Deutsches Edelschwein x Deutsche Landrasse) wurden Untersuchungen zum Einfluß einer porcinen Somatotropin (pST)-Behandlung während des Wachstums auf die chemische Körperzusammensetzung der Tiere, das Adipozyten-Wachstum und das Fettsäure-Profil des Rückenspecks untersucht. Die intramuskulären Injektionen (1 oder 3 mg pST) wurden täglich ab durchschnittlich 65 kg Lebendgewicht bis zum Schlachten verabreicht. Nach der pST-Behandlung wurden bei den Kastraten in allen untersuchten Merkmalen signifikante Unterschiede festgestellt, während sich bei den weiblichen Tieren nur leichte Reaktionen zeigten. Das pST verursachte eine Zunahme des Wasserund Proteingehaltes bei gleichzeitiger Reduzierung des Lipidgehaltes besonders in den fettreichen Körperteilen der Tiere. Im Rückenspeck war der Gehalt an ungesättigten Fettsäuren erhöht und der Fettzelldurchmesser erniedrigt.
    Notes: Summary Seventy eight growing-finishing pigs (male castrates and females) of the cross-breed Pietrain x (Large White x German Landrace) were used to investigate the effects of pST treatment on the chemical composition of the body, the growth of adipocytes, and the fatty acid profile of the backfat. Intramuscular injections (1 or 3 mg pST) were administered daily from an average weight of 65 kg up to slaughter. After pST treatment significant changes in all studied characteristics were observed in barrows, whereas the females exhibited very small responses. The pST caused an increase of water and protein contents and a simultaneous decrease of lipid content especially in body parts rich in fat. Furthermore, the proportion of unsaturated fatty acids increased and the fat cell diameter decreased in the backfat.
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  • 37
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    Journal of productivity analysis 8 (1997), S. 293-310 
    ISSN: 1573-0441
    Keywords: growth ; USagriculture ; externalities ; spill-overs ; public R and D
    Source: Springer Online Journal Archives 1860-2000
    Topics: Economics
    Notes: Abstract Growth in U.S. agriculture is linked to the non-farm economy through domestic terms of trade and factor market adjustments. With almost stable input growth, the relatively large contributions from growth in Total Factor Productivity (TFP) are passed on to intermediate and final consumers in the form of declining real prices for primary farm products. The resulting net growth in the real value of farm output (GDP) is relatively low (0.25% per annum). The decomposition of TFP suggests that public agricultural stock of knowledge and infrastructure are “robustly” associated with TFP growth, while spill-overs from private agricultural and economy wide research and development (R and D) are positive but, relatively small.
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  • 38
    ISSN: 1573-0832
    Keywords: Candida albicans ; ammonium ; physiology ; medium ; growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Candida albicans strain B 311-10 with and without starvation was cultivated in the minimal synthetic medium of Shepherd et al. [18], modified without biotin, aminoacids, low glucose concentration [20] and with decreasing amounts of (NH4)2SO4, to determine the optimal growth requirement for this strain. All the experiments were carried out under sterile conditions at 25 °C in a thermostat with initial O.D.s (675 nm) of 0.500 and 0.100. Cell growth was generally monitored everyday for six days with a spectrophotometer by determining the absorbance of the cultures at 675 nm. All the experiments were repeated three times and a statistical analysis of the data with a probability of 99% and 1% of error was performed to confirm the validity of the results. Best growth was obtained with starved cells at an initial O.D. of 0.100 and with a 0.1 g/L concentration of (NH4)2SO4. At this concentration, the growth of C. albicans B 311-10 was best between the first and the fourth day with the maximum at the third day. With (NH4)2SO4 concentrations of 0.05 and 0.5 g/L, cell growth was the same.
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  • 39
    ISSN: 1573-0832
    Keywords: Epidermophyton floccosum ; E. stockdaleae ; growth ; incubation temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The ability of 17 strains of genus Epidermophyton (15 strains belonging to Epidermophyton floccosum, one to E. floccosum var. nigricans and one to E. stockdaleae) to grow at different temperatures (4 °C, 25 °C, 28 °C, 31 °C, 34 °C, 37 °C and 40 °C) was stated. The strains were inoculated on Sabouraud Dextrose Agar and regularly controled over a period of 14 days when the plates were incubated at 25 °C, 28 °C, 31 °C, 34 °C, 37 °C and 40 °C, and over a period of 70 days when the temperature was 4 °C. The optimal growth of E. floccosum was observed at 28 °C and 31 °C, and no signs of growth were recorded neither at 4 °C nor at 40 °C. The optimal development of E. stockdaleae was observed at 25 °C and 28 °C. This species grew from 4 °C to 31 °C.
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  • 40
    ISSN: 1573-0832
    Keywords: ELISA ; Endodermis ; H. annosum ; Immunocytochemistry ; Root rot ; Vascular tissues
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Vascular disintegration mainly of medulla rays of spruce roots is of major significance in root rot disease of spruce caused byH. annosum. Using seedling roots as an experimental model, the possible routes and initial host reactions preceding invasion of vascular tissues was investigated. Transmission electron microscopy showed that penetration through the endodermis was an obvious route but not without host resistance. Using antibodies againstH. annosum hyphal materials, some labelling of vascular tissues remote from sites of fungal colonization suggest the release of fungal secretory products partly active in tissue disintegration. Similarly, intense labelling was also observed in severely colonized host tissues at late stages of infection. Strong labelling recorded at 3 d p.i. mainly on fungal hyphae and scant gold particles on invaded host tissues could imply that induction of host antifungal metabolites may have been a late event. A correlation was found between total antigenic material in root homogenates measured by ELISA, density of tissue labelling by immunocytochemistry and severity of disease symptoms. The importance of this in relation to diagnosis of biotic root rot diseases in the field is discussed.
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  • 41
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    Nutrient cycling in agroecosystems 16 (1988), S. 217-226 
    ISSN: 1573-0867
    Keywords: Potassium fertilization ; grain sorghum ; savanna soil ; yield ; growth ; varietal difference ; Nigeria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Field trials were conducted at Samaru over a three-year period (1980–82) to study the yield, growth and nutrient concentration of three grain sorghum varieties (L. 187, SK5912 and FFBL) in relation to potassium fertilization in a savanna soil. Potassium application rates were 0, 25, 50 and 75 kg K ha−1. Year × potassium interactions were not significant although there were significant variety × K interactions. The highest grain yields for var. L.187, SK5912 and FFBL occurred from the application of 25, 50 and 75 kg K ha−1 respectively. Straw yield was generally increased by K application, which also promoted tillering and hastened flowering in grain sorghum. Although grain weight per head, head number per m2, grain number and 1000-grain weight were unaffected by this nutrient, weight per head was reduced by 22.8 per cent. K application enhanced N concentration of sorghum plants but caused decline in P concentration. The highest K rate gave the highest K concentration in each of the three varieties at 7 weeks after planting. Optimum K requirement of grain sorghum would seem to be between 25 and 50 kg K ha−1.
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  • 42
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    Nutrient cycling in agroecosystems 40 (1994), S. 243-250 
    ISSN: 1573-0867
    Keywords: Ailanthus excelsa ; biomass ; growth ; N and P fertilizers ; N content ; N uptake ; P content ; P update ; silvicultural efficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A field experiment was conducted on a coarse sand soil having pH 8.8 and organic matter 0.06% in Indian arid region, to study the influence of N and P fertilizers on growth, biomass and nutrient content ofAilanthus excelsa, which is an important fodder species of arid and semi arid regions. Three levels of nitrogen (0, 9 and 18 g N tree−1 as Urea) and of phosphorus (0, 3 and 6 g P2O5 tree−1 as Single Superphosphate) in factorial combinations were taken in triplicate and the experiment was laid in Randomised Block Design. Application of 9 g N plant−1 improved tree height by 15 to 25%, collar circumference by 30 to 37% and crown diameter by 18 to 26% in the initial 3 years. Total biomass increase due to 9 g N plant−1 was 76% and 59%, respectively, after 1 and 2 years of planting. Application of 3 g P2O5 tree−1 increased tree height by 8 to 18% and collar circumference by 17 to 24% during initial three years, and total biomass by 70% at 1 year and 30% at 2 years of age. Combined application of 18 g N and 3 g P2O5 tree−1 (N18P3) was the best treatment which increased tree height by 49%, 85% and 35% and collar circumference by 56%, 10% and 11% at 1, 2, and 3 years of age, respectively. N18P3 treatment increased the total biomass by 181% at 1 year and 185% at 2 years of age. N and P applications improved considerably the branching of roots and root length and enhanced root biomass by 2 to 3 folds. N18P3 treatment increased the nitrogen uptake by 304% (4.02 g tree−1) at 1 year and 211% (42.56 g tree−1) at 2 years of age. The P uptake was maximum (290.4 mg tree−1) due to N18P3 treatment in 1 year old and 11.37 g tree−1 due to N9P6 treatment in 2 year old plantation.
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  • 43
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    Small business economics 14 (2000), S. 195-210 
    ISSN: 1573-0913
    Keywords: growth ; manufacturing ; performance ; product innovation ; small firms
    Source: Springer Online Journal Archives 1860-2000
    Topics: Economics
    Notes: Abstract The paper considers the relative performance [along a number of parameters] of a sample of 228 small manufacturing firms categorised by level of innovation. Whilst innovators appear no more likely to have experienced some form of sales or employment growth, they are significantly more likely to have grown more. In other words, the innovators' growth rate distributions are highly negatively skewed. With regards to export intensities, profitability and productivity levels, the findings are less clear. On the whole, the results reported here are similar to those of other small firm studies, yet vary markedly from large firm equivalents; suggesting that the nature of the returns to innovation may be contingent, at least in part, upon firm size. Moreover, the high levels of variation in firm performance should caution us against proffering innovative imperatives. If we are to counsel firms to "innovate at all costs", we must be clear about, and clearly demonstrate, the nature of the returns they may reasonably expect and the processes through which these may be optimised.
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  • 44
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    Nutrient cycling in agroecosystems 11 (1987), S. 245-266 
    ISSN: 1573-0867
    Keywords: model ; growth ; root/shoot ratio ; nutrients ; light ; winter wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The mathematical analysis presented in this paper of plant growth is based on the assumption that the influence of deficiencies in the major root nutrients of water, N, P and K is to reduce the fraction of the plant mass devoted to photosynthesis by increasing the root fraction, thereby diminishing the rate of growth of the total plant. A reciprocal phenomenon occurs with deficiency of light. Using literature data on the influence of environmental conditions on plant growth and in particular on the root/shoot ratio, the relevant rate expressions are derived in a simple form and appear to be generally similar for a wide range of plants during their vegetative phase of growth.
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  • 45
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    Nutrient cycling in agroecosystems 11 (1987), S. 267-281 
    ISSN: 1573-0867
    Keywords: model ; growth ; root ; soil exploration ; grain fill ; winter wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In the first part of this paper a model is derived for the exploration of the soil by roots in a growing crop with particular reference to cereals. The distribution of root density with depth is related to the growth of the above-ground parts and to the varying demand these place on the roots modified by the changing texture and nutrient status of the soil with depth. In the second part of the paper the growth of cereal seeds is modelled by being related to the photosynthetic capacity of the plant and the duration of its life following anthesis. The death of the plant is considered to be a consequence of the parasitic action of the seeds.
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  • 46
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    Nutrient cycling in agroecosystems 1 (1980), S. 177-190 
    ISSN: 1573-0867
    Keywords: winter wheat ; nitrogen uptake ; autumn dressing ; split-dressing ; growth ; grain yield ; protein content ; harvest-index
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A field experiment with the winter wheat cultivar Donata was carried out on a fine-textured river clay soil in 1978. The rates of nitrogen dressing ranged from 0 to 160 kg N per ha and were split over from one up to three application times: autumn, early spring and late spring. Total above-ground dry matter and grain dry-weight yields ranged from 9.1 to 13.7 tons per ha and from 4.17 to 6.35 tons per ha, respectively. Late top-dressings increased the harvest-index, whereas an autumn dressing had the opposite effect. Number of culms per m2, grain weight (mg) and grain number per m2 increased from 350 to 430, from 35.5 to 36.8 and from 11 680 to 16 980, respectively, as the nitrogen dosage was raised from 0 to 160 kg N per ha. The linear rate of grain growth ranged from 111 to 172 kg dry matter per ha per day with nitrogen doses from 0 to 160 kg N per ha. Differences in rate of grain growth per unit area were mainly related to number of grains per m2. The association between grain number and grain yield was reflected by a correlation coefficient of 0.97 (n = 32). A higher level of nitrogen dressing enhanced the leaf area index and leaf area duration. However, we could not derive an effect of nitrogen on the duration of grain growth. Total nitrogen yield ranged from 71 to 166 kg N per ha and grain nitrogen yield from 54 to 122 kg N per ha with nitrogen dosages of 0 and 160 kg N per ha, respectively. The nitrogen concentration of the grains varied between 1.3 and 2.0 N. An autumn dressing of 40 kg N per ha generally showed only minor effects on yield and yield components. Top dressings during spring resulted in a higher recovery and efficiency of the applied nitrogen. Therefore, it may be concluded from this experiment and literature that on fertile soils an autumn dressing of nitrogen will not be economical, but split-dressings in spring are very beneficial. In particular, a late nitrogen application during the boot stage increased grain number, harvest-index and grain yield as well as protein concentration of the grain.
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  • 47
    ISSN: 1573-0867
    Keywords: Zea mays L. ; nitrogen fertilization ; plant density ; yield components ; growth ; micronutrient nutrition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Growth and yield components in field-grown maize (Zea mays L.) were enhanced by nitrogen fertilization ranging from 50 to 200 kg N ha−1. Ear diameter, kernel depth, grain: stover ratio, number of ears plant−1, plant height and dry matter production increased as N fertilization rate was increased up to 100 or 150 kg N ha−1. Tasselling in maize was hastened by N fertilization. Increasing plant density from 25000 to 75000 plants ha−1 increased plant height, dry matter production and delayed tasseling but reduced ear diameter, kernel depth, grain: stover ratio and number of ears plant−1. Increased N supply and plant density had no influence on the concentrations of Mn, Zn, Cu, and Fe in ear leaf; except that Mn concentration increased as N fertilization rate was increased up to 150 kg N ha−1. Nitrogen × plant density interactions on the concentrations of the micronutrients in maize ear leaf were not significant.
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  • 48
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    Nutrient cycling in agroecosystems 3 (1982), S. 25-36 
    ISSN: 1573-0867
    Keywords: iodine ; lucerne ; ryegrass ; growth ; chemical composition ; nutrient-solutions ; ammoniacal and nitrate nitrogen ; acidity ; volatilization ; fertilizers ; grazing animals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Growth of lucerne was inhibited by sodium iodide even more than that of ryegrass above a concentration equivalent to 1µg cm−3 of iodine in an applied solution. In sand-culture the extent to which the iodide depressed yield of dry matter depended on the type of nutrient-solution used to stimulate and maintain vigorous growth. In contrast, where the plants were grown in soil the depression was independent of the nutrient-solution used. This difference between solutions is a reflection of the extent to which growing plants can modify pH in the root-zone of a sandy unbuffered substrate and leads to a conclusion that acidity greatly enhances the toxic effect of the iodine. There are reasons for believing that in sunlight iodine may be lost by volatilization even from living plant-tissue. The implication of these findings is discussed in relation to using either iodized fertilizers or iodized salt-blocks in order to maintain iodine in pastoral systems at levels satisfactory for animal-health.
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    Mycopathologia 125 (1994), S. 107-117 
    ISSN: 1573-0832
    Keywords: Aflatoxin B1 ; Immunocytochemistry ; Regeneration ; Tissue culture ; Tobacco plantlets
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The effects of aflatoxin B1, (0.5–25 µg ml−1) on in vitro root and shoot development in young tobacco explants were investigated. Despite an initial apparent stimulatory effect on most measured parameters at 0.5 µg ml−1 AFB1, the number of leaves, root and leaf mass per plantlet were progressively inhibited with increasing AFB1 concentration. The number of explants developing roots was reduced to 34% at the highest (25 µg ml−1) AFB1 concentration, following 3 weeks exposure to the toxin. Leaf chlorophyll content at this toxin concentration was significantly lower than that measured for control plantlets. Thin layer chromatography confirmed the absorption of AFB1 by the plantlets. Using immunocytochemical techniques, AFB1 was immunolocated predominantly in the vacuoles, the nucleus and the cytoplasm (possibly intravesicularly). The results are discussed in terms of this immunolocation within the cell.
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  • 50
    ISSN: 1573-0603
    Keywords: growth ; differentiation ; mammary epithelium ; collagen gel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mammary glands are enzymatically dissociated and the resulting tissue digest enriched for epithelial cells by isopycnic banding on a density gradient of Percoll. The cells are embedded within a rat tail collagen gel matrix and fed with the appropriate medium. Growth and differentiation are superior in such a system when compared to culture on plastic, using identical media.
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  • 51
    ISSN: 1573-0832
    Keywords: mosquito pathogen ; growth ; sporulation ; temperature ; pH ; salinity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The New Zealand strain of Tolypocladium cylindrosporum was cultured on Sabouraud dextrose agar medium under varying regimes of growth conditions. The isolate exhibited good tolerances to temperature (4–35 °C), pH (3–10) and salinity (0–7% NaCl). Optimal vegetative growth and sporulation were recorded between a temperature range of 20–30° C, pH of 5–6 and a salinity level of 0–2% NaCl. The North American isolate of the fungus showed similar tolerances, while the European isolate was less tolerant.
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  • 52
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    Nutrient cycling in agroecosystems 39 (1994), S. 217-222 
    ISSN: 1573-0867
    Keywords: growth ; K accumulation ; K nutrition ; physiological efficiency ; soybean ; yield
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Field experiments were conducted during 1989 to 1991 on a loamy sand (Typic ustochrept) soil to study the K nutrition of soybean. Soybean responded significantly up to 50 kg K ha−1 when applied 1/2 of K at planting and 1/2 at flower initiation (two split) or applying 1/3 K at planting, 1/3 at flower initiation and 1/3 at pod development (three splits). Split application was more beneficial than applying full K at time of planting due to higher leaf area index, crop growth rate, chlorophyll content of fresh leaves, K accumulation in soybean and better agronomic and physiological efficiency of applied K. Agronomic efficiency, physiological efficiency and apparent recovery of K reduced as rate of applied K was increased from 50 to 75 kg ha−1. Highest K+ concentration (3.4 % of dry matter) was recorded in 30-day-old plants at 75 kg K ha−1 which depressed progressively with the age of the crop. At maturity, the K concentration of soybean seed varied from 1.5% (unfertilised K plants) to 2.1 % (when 75 kg K ha−1 was applied in three splits).
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  • 53
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    Journal of statistical physics 74 (1994), S. 91-109 
    ISSN: 1572-9613
    Keywords: Random process ; local interaction ; critical phenomena ; invariant distribution ; growth ; eroder ; convexity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Components which are placed in a finite or infinite space have integer numbers as possible states. They interact in a discrete time in a local deterministic way, in addition to which all the components' states are incremented at every time step by independent identically distributed random variables. We assume that the deterministic interaction function is translation-invariant and monotonic and that its values are between the minimum and the maximum of its arguments. Theorems 1 and 2 (based on propositions which we give in a separate Part II), give sufficient conditions for a system to have an invariant distribution or a bounded mean. Other statements, proved herein, provide background for them by giving conditions when a system has no invariant distribution or the mean of its components' states tends to infinity. All our main results use one and the same geometrical criterion.
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  • 54
    ISSN: 1572-977X
    Keywords: Avicennia alba ; growth ; internodal sequences ; mangrove age determination ; Rhizophora apiculata ; Sonneratia caseolaris ; Thailand
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The occurrence of periodic changes in the length of the internodes of the seedlings of SE Asian mangrove species (Rhizophora apiculata, Avicennia alba, and Sonneratia caseolaris) growing in the Pak Phanang estuary (Nakhon Si Thammarat, SE Thailand) was confirmed. The annual nature of these periodic changes was verified through comparisons with the observed average number of internodes produced by plants tagged for one year. The cycles in the sequence of internodal length of the seedlings had an average (± SE) period of 16.0 ± 0.78 internodes for Avicennia alba, 25.0 ± 1.2 internodes for Sonneratia caseolaris, and 8.29 ± 0.40 internodes for Rhizophora apiculata. These values are remarkably close to, and not significantly different (t‐test, P 〉 0.05) from the average (± SE) annual number of internodes of 17.6 ± 0.8 for Avicennia alba, 28.8 ± 2.1 for Sonneratia caseolaris, and 8.03 ± 0.36 for Rhizophora apiculata produced in a year by tagged plants. In addition, the average (± SE) growth of the tagged plants was 0.39 ± 0.018 cm day-1 for Avicennia alba, 0.24 ± 0.015 cm day-1 for Sonneratia caseolaris, and 0.091 ± 0.0036 cm day-1 for Rhizophora apiculata. These estimates were very close to those (0.45 ± 0.06 cm day-1 for Avicennia alba, 0.32 ± 0.01 cm day-1 for Sonneratia caseolaris, and 0.13 ± 0.005 cm day-1 for Rhizophora apiculata) derived as the product of the mean number of internodes produced per day, calculated from the inferred number of internodes produced per year, and the mean length of the internodes of the plants. These findings indicate that the use of regular, annual changes in the length of the internodes of mangrove seedlings can be used reliably to determine their age and to estimate their growth rate.
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  • 55
    ISSN: 1432-041X
    Keywords: Vitellin ; Yolk granule ; Yolk protein ; Silkworm ; Embryogenesis ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Vitellin was purified from eggs of the silkworm,Bombyx mori, by a new method in which vitellin was extracted from isolated yolk granules. The purified vitellin had a molecular weight of 540,000. An antibody against purified vitellin was prepared in rabbits. It reacted with the hemolymph vitellogenin as well as with purified vitellin, but not with other proteins in the hemolymph or in the extract from yolk granules. The anti-vitellin IgG was used to immunocytochemically locate vitellin in theBombyx non-diapause egg during early developmental stages. In the egg, just after oviposition, vitellin was located in internal yolk granules and in small yolk granules of the periplasm. During the early developmental stages studied, vitellin was not metabolized uniformly throughout the egg. The vitellin of the internal yolk granules located at the posterior-dorsal part and of the small peripheral yolk granules was utilized in 16 h and 2 days, respectively, after oviposition. A thin, very vitellin-poor layer was located between the periplasm and the vitellin-rich interior in the newly laid egg. it was always in close contact with the periphery where blastoderm and germ-band cells developed.
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  • 56
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    Cellular and molecular life sciences 41 (1985), S. 353-354 
    ISSN: 1420-9071
    Keywords: Teleost fish, freshwater ; muscle, white myotomal ; myotomal muscle ; muscle fibers ; fish size ; growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Dynamics of increase of white myotomal muscle fibers of four species of freshwater teleosts (Salmo gairdneri, Pimephales notatus, Esox masquinongy andE. americanus vermiculatus) from three families (Salmonidae, Cyprinidae, Esocidae) representing a variety of maximum attainable sizes and growth rates, have been investigated. There are at least three major differences in these dynamics, and there appears to be an association between the ability of a fish species to attain large size (and grow fast) and its ability to recruit new fibers into this predominant tissue of the myotomal mass.
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  • 57
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    Cellular and molecular life sciences 44 (1988), S. 776-777 
    ISSN: 1420-9071
    Keywords: Fourier analysis ; growth ; selection ; size ; shape
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Fourier analysis of videodigitised outlines of mouse vertebrae from two stocks, a pseudo-longitudinal series of mice aged 25–60 days and one selected for large or small body size over many generations shows that the shape changes due to normal growth are not similar to those produced by selection for body size.
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  • 58
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    Aquatic sciences 51 (1989), S. 296-305 
    ISSN: 1420-9055
    Keywords: Arctic char ; growth ; maturation ; reoligotrophication ; Lake Walenstadt
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Within one decade the yield in arctic char fishery of Lake Walenstadt (Switzerland) has dropped from 1.7 kg · ha−1 to 0.7 kg · ha−1. In the same period growth decreased by about 15%. The fish considered as “degenerated dwarfed chars” by fishermen are supposed to mature in very young ages. The change of the growth pattern can be related to reoligotrophication of the lake and impoverishments of the food source. Compared to other arctic char populations in Switzerland maturation takes place at least one year later.
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  • 59
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    Aquatic sciences 55 (1993), S. 76-86 
    ISSN: 1420-9055
    Keywords: Sa velinus alpinus ; growth ; Lake Geneva ; latitude ; eutrophication ; fish density
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The growth rate of the Arctic charr,Salvelinus alpinus (L.), is exceptionally high in Lake Geneva; one of the highest every recorded. This can be explained by three factors: (1) the Arctic charr of Lake Geneva is the most southerly indigenous population in the world, (2) the trophic resources are important because of the eutrophication of the lake, (3) the fish density is low.
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  • 60
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    Aquatic sciences 56 (1994), S. 70-79 
    ISSN: 1420-9055
    Keywords: Macrophytes ; growth ; assimilation ; macroelements
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Helophytes are often incorporated into biological wastewater treatment plants. In favourable situations, they can take up large amounts of nutrients. One helophyte, the reed canarygrass (Phalaris arundinacea L.), is fast growing when supplied with sufficient light and nutrients. Experiments were carried out under natural climatic conditions in small plastic tanks filled with sand with regular additions of a balanced and concentrated nutrient solution. In the growing season (May–October), plant production reached 10.5 kg m−2 of dry biomass, of which 66% was in the aerial parts. Maximum nutrient uptake capacity was reached just after flowering and before senescence (beginning of October): 49% N, 34% P, 52% K and 34% Mg of the input was fixed in the aerial parts, which are easily harvestable. The corresponding values for the below ground parts were 12%, 10%, 11% and 11% respectively. Excretion of K and Mg has been observed when nutrients are translocated to the storage organs.
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  • 61
    ISSN: 1432-041X
    Keywords: Major haemolymph proteins ; Development ; Cuticle ; Immunocytochemistry ; Ceratitis capitata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The developmental profile of the major haemolymph proteins (ceratitins) inCeratitis capitata was studied. Ceratitin concentration in the haemolymph decreases dramatically during the last days of pupal life, while the amounts of ceratitins in whole organism extracts remain unchanged. By electrophoretic, immunological and immunofluorescence techniques it was revealed that ceratitins are reabsorbed by the fat body and a fraction of them is deposited in the cuticle. The possible role of ceratitins is discussed.
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  • 62
    ISSN: 1432-041X
    Keywords: Sea urchin ; Jelly coat ; Accessory cell ; Oogenesis ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The immunocytochemical localization of the egg-jelly macromolecule, a fucose sulphate glycoconjugate (FSG) that induces the acrosome reaction in spermatozoa, was investigated in ovaries of the sea urchin Hemicentrotus pulcherrimus by use of a polyclonal antibody. The polyclonal antibody reacted with the accessory cells and oocytes in the ovarian lumen. In the accessory cells, evidence of an intense immunohistochemical reaction was observed in many globules of variable density. Products of the specific immunohistochemical reaction were frequently observed in the surface region of oocytes, at a distance from the ovarian wall. At the ultrastructural level, the polyclonal antibody was found to react with the material present in the vacuole-like structures of the globules in the accessory cells. Many gold particles, demonstrating specific immunolabelling, were associated with well-developed microvilli on the vitellogenic oocytes. In the mature oocytes, intense labelling was observed in the jelly coat but not in the vitelline coat. By contrast, oogonia and early oocytes were barely labelled. Quantitative data indicated that the extent of immunolabellings in the surface region of oocytes was very high in the vitellogenic and mature oocytes. In all cases, neither the oocyte cytoplasm nor the subcellular organelles were labelled. These results suggest that FSG is produced by the accessory cells and is deposited initially on the surface of vitellogenic oocytes for the formation of jelly. These findings may provide a new insight into the role of the accessory cells in the reproductive process of the sea urchin.
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  • 63
    ISSN: 1432-041X
    Keywords: Sea urchin ; Egg jelly ; Ovary ; Development ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In the sea urchin Hemicentrotus pulcherrimus, the egg-jelly macromolecule, a fucose sulphate glycoconjugate (FSG) that induces the acrosome reaction in spermatozoa, originates from the accessory cells in the ovary. In the present study we examined the seasonal variations in the distribution of FSG in the ovary by immunocytochemistry with a polyclonal antibody. An enzyme-linked immunosorbent assay indicated that FSG was present in supernatants of extracts of ovaries throughout the development of the ovary. However, the immunohistochemical study showed that there are marked seasonal changes in the distribution of FSG in ovaries. The polyclonal antibody reacted strongly with globules of accessory cells before the beginning of the breeding season (August to December). During the breeding season (February to April), the immunohistochemical reaction was found on the surface of oocytes but was weak in the accessory cells. At the ultrastructural level, the antibody reacted with globules of variable density in accessory cells. Intense immunolabelling was observed in the vacuole-like structures of the globules. Sometimes, products of the specific immunocytochemical reaction were found in the Golgi apparatus in these globules. Quantitative examination indicated that FSG was actively produced by the accessory cells from the late non-breeding season to the pre-breeding season. These results suggest that there are marked seasonal variations in the production of FSG by the accessory cells in the sea urchin ovary. These findings also provide new evidence that accessory cells exhibit dynamic changes during the reproductive process in the sea urchin.
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  • 64
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    Cellular and molecular life sciences 41 (1985), S. 1340-1342 
    ISSN: 1420-9071
    Keywords: Immunocytochemistry ; calmodulin ; secretory granules
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Calmodulin is a regulator of several calcium-dependent cellular processes. It has been suggested that it plays a role in the mechanism of secretion. Employing an indirect immunoperoxidase technique at the light microscope level, this study demonstrates the presence of calmodulin in several exocytotic cells (mast cells, thyroid follicular cells, neurohypophyseal neurosecretory terminals, pancreaticβ-cells and pancreatic acinus cells) in rat and man. The positive staining reaction for calmodulin was granular and at least in the case of rat mast cells it appeared to be associated with the granule membrane.
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  • 65
    ISSN: 1420-9071
    Keywords: Serratia marcescens ; growth ; prodigiosin ; prodigiosin condensing enzyme ; secondary metabolites
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Prodigiosin condensing enzyme (PCE) activities were present inSerratia marcescens wild type 08, mutants OF, WF and 9-3-3. Their specific activities exhibited different maxima and at different times during the late log phase or the early stationary phase of cell growth. The levels of prodigiosin and its precursors also showed a significant increase at this period. The results support that prodigiosin and/or its precursors are secondary metabolites. The ubiquity of the PCE activity in mutants deficient in prodigiosin biosynthesis suggest that this particular enzyme may also be present in non-pigmented clinical isolates.
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  • 66
    ISSN: 1420-9071
    Keywords: Alternating high magnetic fields ; eddy current ; magnetic flux concentration ; Caenorhabditis elegans ; growth ; reproduction ; behavior
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Here we describe a device for testing possible influences of high magnetic fields on biological processes, by which alternating-current magnetic stimuli as high as 1.7 T can be administered. Experiments with a simple multicellular organism, the nematodeCaenorhabditis elegans, revealed that intermittent exposure to the magnetic fields modestly inhibited the animal's reproduction as well as its post-embryonic development, and caused a marked but transient derangement in its locomotory behavior. Available evidence indicates that alternating high magnetic fields can elicit both chronic and acute biological effects, but that the effects may be well tolerated or compensated for by the living organism.
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  • 67
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    Trees 13 (1999), S. 138-151 
    ISSN: 0931-1890
    Keywords: Key words Cytoskeleton ; Immunocytochemistry ; Model systems ; Populus ; Secondary vascular system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  Trees represent a, probably the, major component of the biosphere and have a unique place in the history of Mankind. One of their most fascinating features is the process of secondary growth which is effected principally by the secondary vascular system, the developmental continuum of secondary phloem, vascular cambium, and secondary xylem. However, for too long assumptions about the developmental biology of trees have had to be based upon studies of primary growth systems within annual, herbaceous species because study of the secondary vascular system had been largely ignored. Even when attempts are made to understand some of the most fundamental features of the secondary vascular system, such as xylogenesis, the current model system, isolated Zinnia mesophyll cells, is not entirely appropriate to the situation in the intact tree. Some deficiencies of the Zinnia system are discussed, and the advantages of the genus Populus as a model for study of the hardwood secondary vascular system are considered. Some of the new approaches which are poised to lead to significant advances in our knowledge of the cell bio-logy of the secondary vascular system of trees – spe-cifically of the cell wall, the plasmalemma, and the cytoskeleton – are discussed. The value of one of these new techniques – immunocytochemistry – is demonstrated by a consideration of recent work on the role of the cytoskeleton in the hardwood secondary vascular system.
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  • 68
    ISSN: 1432-0827
    Keywords: Androgen Receptor ; Osteoclast ; Mouse ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Expression of androgen receptor (AR) in mouse osteoclast-like multi-nucleated cells (OCs) was examined with immunocytochemical techniques. Murine OCs were obtained by co-culturing mouse osteoblastic cells and bone marrow cells. Three preparations of polyclonal anti-AR antibody which were raised in rabbit against different parts of the human AR were employed for the experiments. Specific staining for AR was demonstrated in the nuclei and the perinuclear area of mouse OCs. This is the first report demonstrating the presence of AR in osteoclast-like cells.
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  • 69
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    Calcified tissue international 24 (1977), S. 223-229 
    ISSN: 1432-0827
    Keywords: Enamel-cementum-morphology ; Immunocytochemistry ; Biochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The continuously erupting rabbit incisor tooth is normally thought of as having an enamel covered “crown” on its labial surface and a cementum covered “root” on its lingual surface. We have examined both surfaces of continuously erupting rabbit incisor teeth taken from near term embryos by a variety of means, including transmission and scanning electron microscopy, biochemical fractionation, and immunohistochemistry. In all cases, we could detect no qualitative difference in the early extracellular matrices taken from the labial and lingual surfaces of the teeth. Both matrices were shown to be composed of dentin and enamel, although the thickness and geometry of the enamel matrix on the lingual surface was somewhat different from that on the labial surface.
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  • 70
    ISSN: 1432-0827
    Keywords: Growth hormone ; Growth hormone receptor ; Odontogenesis ; Bone remodeling ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Growth hormone (GH) may regulate tooth formation and bone remodeling associated with tooth eruption. This study reports the distribution of growth hormone receptor/binding protein in developing rat molars and adjacent alveolar bone by immunocytochemistry using well-characterized anti-growth hormone receptor monoclonal antibodies. These tissues represent an excellent model for studying the ontogenic changes that occur in odontogenic and osteogenic cells, as these cells are found in linear arrays displaying the various stages of morphological and functional differention, and differentiated function. Immunoreactivity was first seen in precementoblasts in contact with the epithelial root sheath, and preodontoblasts. However, growth hormone receptor immunoreactivity was associated primarily with the cytoplasm of odontogenic and osteogenic cells forming their respective matrices. Thus, cementoblasts and odontoblasts at sites of new matrix formation showed intense immunoreactivity whereas cementocytes and mature odontoblasts at later stages of tooth development were nonreactive. Osteoblasts engaged in intramembranous ossification in the alveolar bone were positive, although osteocytes and endosteal cells were immunonegative. Osteoclasts at sites of alveolar bone remodeling resorption were also immunopositive. These patterns of receptor expression parallel the ontogenic sequences of odontogenic and osteogenic cells and suggest that GH promotes the functional state of these cells. Our results also imply that GH may influence differentiation or differentiated functions associated with odontogenesis, osteogenesis, and bone remodeling independent of systemic insulin-like GF-I.
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  • 71
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    Histochemistry and cell biology 106 (1996), S. 9-17 
    ISSN: 1432-119X
    Keywords: Silver enhancement ; Immunogold-silver staining ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The immunogold silver staining method (IGSS) is widely used as a sensitive and specific immunohistochemical visualisation technique. IGSS involves the specific deposition of metallic silver at the site of immunogold labelling and provides a means of visualisation at low magnification by light or electron microscopy. Silver developers for IGSS rapidly deposit metallic silver only at the site of heavy metals, including gold and silver, because of their catalytic activity. The developing solution contains the silver ions and reducing agent necessary for this reaction. Using different silver salts as ion donors and by selecting an appropriate temperature and pH, visible amounts of silver can be deposited in a few minutes at the site of colloidal gold labelling while little non-specific background deposition occurs. Inclusion of protective colloids in the solution can also be used to control the reaction. Although studies of the chemical basis of silver deposition around unlabelled colloidal gold date back to 1939, immunogold enhancement by silver was established in 1983. The IGSS method evolved from the combination of disparate photographic, histochemical and immunogold techniques which have been effectively combined and optimised over the last 10 years to provide a visualisation system which is well suited to many immunohistochemical studies.
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    Histochemistry and cell biology 106 (1996), S. 9-17 
    ISSN: 1432-119X
    Keywords: Key words Silver enhancement ; Immunogold-silver staining ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  The immunogold silver staining method (IGSS) is widely used as a sensitive and specific immunohistochemical visualisation technique. IGSS involves the specific deposition of metallic silver at the site of immunogold labelling and provides a means of visualisation at low magnification by light or electron microscopy. Silver developers for IGSS rapidly deposit metallic silver only at the site of heavy metals, including gold and silver, because of their catalytic activity. The developing solution contains the silver ions and reducing agent necessary for this reaction. Using different silver salts as ion donors and by selecting an appropriate temperature and pH, visible amounts of silver can be deposited in a few minutes at the site of colloidal gold labelling while little non-specific background deposition occurs. Inclusion of protective colloids in the solution can also be used to control the reaction. Although studies of the chemical basis of silver deposition around unlabelled colloidal gold date back to 1939, immunogold enhancement by silver was established in 1983. The IGSS method evolved from the combination of disparate photographic, histochemical and immunogold techniques which have been effectively combined and optimised over the last 10 years to provide a visualisation system which is well suited to many immunohistochemical studies.
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  • 73
    ISSN: 1432-1939
    Keywords: Key words Agrostis canina ; CO2 vents ; photosynthesis ; lignification ; growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The aim of this study was to characterise growth and photosynthetic capacity in plants adapted to long-term contrasting atmospheric CO2 concentrations (C a). Seeds of Agrostis canina L. ssp. monteluccii were collected from a natural CO2 transect in central-western Italy and plants grown in controlled environment chambers at both ambient and elevated CO2 (350 and 700 μmol mol−1) in nutrient-rich soil. Seasonal mean C a at the source of the plant material ranged from 610 to 451 μmol CO2 mol−1, derived from C4 leaf stable carbon isotope discrimination (δ13C). Under chamber conditions, CO2 enrichment stimulated the growth of all populations. However, plants originating from elevated C a exhibited higher initial relative growth rates (RGRs) irrespective of chamber CO2 concentrations and a positive relationship was found between RGR and C a at the seed source. Seed weight was positively correlated with C a, but differences in seed weight were found to explain no more than 34% of the variation in RGRs at elevated CO2. Longer-term experiments (over 98 days) on two populations originating from the extremes of the transect (451 and 610 μmol CO2 mol−1) indicated that differences in growth between populations were maintained when plants were grown at both 350 and 700 μmol CO2 mol−1. Analysis of leaf material revealed an increase in the cell wall fraction (CWF) in plants grown at elevated CO2, with plants originating from high C a exhibiting constitutively lower levels but a variable response in terms of the degree of lignification. In vivo gas exchange measurements revealed no significant differences in light and CO2 saturated rates of photosynthesis and carboxylation efficiency between populations or with CO2 treatment. Moreover, SDS-PAGE/ LISA quantification of leaf ribulose bisphosphate carboxylase/oxygenase (Rubisco) showed no difference in Rubisco content between populations or CO2 treatments. These findings suggest that long-term adaptation to growth at elevated CO2 may be associated with a potential for increased growth, but this does not appear to be linked with differences in the intrinsic capacity for photosynthesis.
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  • 74
    ISSN: 1432-2048
    Keywords: Immunocytochemistry ; Lectin (localization) ; Phaseolus (lectin) ; Phytohemagglutinin ; Seed (lectin)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have examined the properties and subcellular localization of phytohemagglutinin (PHA), the major lectin of the common bean (Phaseolus vulgaris.), in the axis cells of nearly mature and imbibed mature seeds. On a protein basis the axis contained about 15% as much PHA as the cotyledons. Localization of PHA was done with an indirect immunolabeling method (rabbit antibodies against PHA, followed by colloidal gold particles coated with goat antibodies against rabbit immunoglobulins) on ultra-thin cryosections which were embedded in plastic on the grids after the immunolabeling procedure. The embedding greatly improved the visualization of the subcellular structures. The small (4 nm) collodial gold particles, localized with the electron microscope, were found exclusively over small vacuoles or protein bodies in all the cell types examined (cortical parenchyma cells, vascular-bundle cells, epidermal cells). The matrix of these vacuoles-protein bodies appears considerably less dense than that of the protein bodies in the cotyledons, but the results confirm that in all parts of the embryo PHA is localized in similar structures.
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  • 75
    ISSN: 1432-203X
    Keywords: wheat ; rye ; embryogenesis ; growth ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The influence of the short arm of rye chromosome 1 (1RS) from Secale cereale var. Imperial on the growth and differentiation of callus cultures from wheat Triticum aestivum var. Chinese Spring immature embryos was analysed. This chromosome arm was found to stimulate both embryogenesis and the rate of growth of calli. Recombinant lines carrying segments of 1RS were used to delineate the regions of 1RS responsible for the tissue culture effects. The enhancement of embryogenesis and the stimulation of growth were shown to be associated with two distinct genetic regions of the chromosome arm; the former is located between the centromere and the Sec 1 locus, while the latter is situated in the immediate vicinity of the Sec 1 locus.
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  • 76
    ISSN: 1432-2048
    Keywords: Glycine (xanthine dehydrogenase) ; Immunocytochemistry ; Polyclonal antibody ; Root nodule ; Xanthine dehydrogenase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Xanthine dehydrogenase (XDH, EC 1.2.1.37) was purified from root nodules of soybean (Glycine max) and used to prepare a polyclonal rabbit antiserum. Monospecificity of this antiserum was ascertained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the immunoprecipate. During root nodule development of soybean, only one form of XDH was detected on an immunological basis. Titration of XDH by immunoelectrophoresis showed that a remarkable increase in the amount of XDH occurred between two and four weeks after inoculation, in parallel with the increase in enzyme activity. Localization of XDH by immunofluorescence indicated that the enzyme was present exclusively in uninfected cells where it appeared to be associated with discrete organellels
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  • 77
    ISSN: 1432-2048
    Keywords: Avena ; Immunocytochemistry ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phytochrome of oat (Avena sativa L., cv. Garry) coleoptile cells in the red-light-absorbing form, Pr, is diffusely distributed while after conversion to the far-red-light-absorbing form, Pfr, it is observed only in very small areas within the cell. Comparison of phytochrome photoversibility measurements to the distribution of the pigment within the cell indicates that the spectral assay is not influenced by the observed compartmentalization of the chromoprotein. However, the observed compartmentalization of phytochrome is correlated with a loss in spectrophotometrically detectable Pr.
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  • 78
    ISSN: 1432-2048
    Keywords: Cotyledons ; Endoplasmic reticulum ; Ferritin labeling ; Immunocytochemistry ; Phaseolus ; Protein (reserve) ; Reserve protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The ultrastructure of the storage parenchyma cells of the cotyledons of developing bean (Phaseolus vulgaris L.) seeds was examined in ultrathin frozen sections of specimens fixed in a mixture of glutaraldehyde, formaldehyde and acrolein, infused with 1 M sucrose, and sectioned at-80° C. Ultrastructural preservation was excellent and the various subcellular organelles could readily be identified in sections which had been stained with uranyl acetate and embedded in Carbowax and methylcellulose. The cells contained large protein bodies, numerous long endoplasmic reticulum cisternae, mitochondria, dictyosomes, and electron-dense vesicles ranging in size from 0.2 to 1.0 μm. Indirect immunolabelling using rabbit immunoglobulin G against purified phaseolin (7S reserve protein), and ferritin-conjugated goat immunoglobulin G against rabbit immunoglobulin G was used to localize phaseolin. With a concentration of 0.1 mg/ml of anti-phaseolin immunoglobin G, heavy labeling with ferritin particles was observed ober the protein bodies, the cisternae of the endoplasmic reticulum, and the vesicles. The same structures were lightly labeled when the concentration of the primary antigen was 0.02 mg/ml. Ferritin particles were also found over the Golgi bodies. The absence of ferritin particles from other organelles such as mitochondria and from areas of cytoplasm devoid of organelles indicated the specificity of the staining, especially at the lower concentration of anti-phaseolin immunoglobulin G.
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  • 79
    ISSN: 1432-2048
    Keywords: Immunocytochemistry ; (PEP carboxylase) ; PEP carboxylase ; Sorghum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The localization of phosphoenol pyruvate carboxylase (EC 4.1.1.3.1.) in the leaf cells of Sorghum vulgare was investigated by using three techniques: the conventional aqueous and non aqueous methods gave conflicting results; the immunocytochemical techniques clearly showed that the enzyme is predominantly located in the cytoplasm of mesophyll cells.
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  • 80
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    Oecologia 71 (1987), S. 486-490 
    ISSN: 1432-1939
    Keywords: Helianthemum ; growth ; flowering ; age ; drought response
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The hills of the Negev highlands, west of Sede Boqer, are typically covered by various half-shrub communities, including the deciduous species of Helianthemum vesicarium Boiss., an Irano-Turanian element that grows only on north facing slopes, and H. ventosum Boiss., a Saharo-Arabian element which grows on both slopes but mainly on those facing south. Upon irrigation, plants of H. vesicarium preserve their natural rhythm of activity during winter and remain deciduous throughout the summer. On the other hand, under a similar irrigation treatment, the growth and flowering season of H. ventosum is modified and is extended well into the summer. Thus, H. ventosum shows a high phenological plasticity, while H. vesicarium seems to be very conservative. The different response of the two species to an improved water regime may partly explain the differences in their natural habitats: H. vesicarium on the more humid north facing slopes and H. ventosum mostly on the more arid and extreme south facing ones. Plants of both species which grow near boulders attain larger sizes and denser stands than those on the slopes. Age analysis based on xylem ring counts of 859 plants of the two natural populations showed that the plants did not exceed 14 years and most of them reached the age of some 5 years only. In general more seedlings are established during rainy year-clusters than during dry ones. However, no direct correlation between seedling establishment and the annual rainfall of specific years could be found. The rapid turnover of plants in the stands of both species of Helianthemum resembles the behavior of desert annuals rather than that of most desert shrubs.
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  • 81
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    Sexual plant reproduction 5 (1992), S. 89-100 
    ISSN: 1432-2145
    Keywords: Actin ; Cytoskeleton ; Generative cell ; Immunocytochemistry ; Microtubule ; Mitosis ; Phragmoplast ; Pollen ; Rhodamine phalloidin ; Sperm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Disagreement has arisen over the presence of actin-containing microfilaments (Mfs) in angiosperm generative cells and sperm (GSP). In order to address this issue, we subjected GSP of Tradescantia virginiana, Nicotiana tabacum and Rhododendron laetum to a series of localizations using different antiactins, rhodamine phalloidin and antimyosin. Coordinate staining with antitubulin and Hoechst 33258 defined the status of the microtubule (Mt) cytoskeleton and stages of generative cell division. Additional experiments utilized cytochalasin D (CD). In no instance could Mfs be detected in GSP of the three species. Instead, Mfs seen at the periphery of GSP appear to be continuous with vegetative Mfs and thus are in the vegetative cytoplasm. Mfs are not seen in the constriction zone of dividing T. virginiana generative cells, nor are they indicated in the phragmoplast of N. tabacum and R. laetum. Myosin localizations reveal punctate staining in the vegetative cytoplasm and a thin line of fluorescence around the the outside of the generative cell. While CD seems to delay generative cell division, cytokinesis still takes place. CD-induced Mf fragments are evident in the vegetative cytoplasm but not in GSP. The weight of evidence therefore indicates that GSP do not contain Mfs. The implications of this conclusion for the behavior of GSP and the mechanism of cytokinesis in dividing generative cells are considerable.
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  • 82
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    Journal of comparative physiology 169 (1991), S. 39-50 
    ISSN: 1432-1351
    Keywords: Photoreception ; Retinally degenerate ; Mouse ; Circadian ; Rods ; Cones ; 11-cis retinaldehyde ; Immunocytochemistry ; HPLC
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary We have examined the effects of light on circadian locomotor rhythms in retinally degenerate mice (C57BL/6J mice homozygous for the rd allele: rd/rd). The sensitivity of circadian photoreception in these mice was determined by varying the irradiance of a 15 min light pulse (515 nm) given at circadian time 16 and meauring the magnitude of the phase shift of the locomotor rhythm. Experiments were performed on animals 80 days of age. Despite the loss of visual photoreceptors in the rd/rd retina, animals showed circadian responses to light that were indistinguishable from mice with normal retinas (rd/+ and +/+). While no photoreceptor outersegments were identified in the retina of rd/rd animals (80–100 days of age), we did identify a small number of perikarya that were immunoreactive for cone opsins, and even fewer cells that contained rod opsin. Using HPLC, we demonstrated the presence and photoisomerization of the rhodopsin chromophore 11-cis retinaldehyde. The rd/rd retinas contained about 2% of 11-cis retinaldehyde found in +/+ retinas. We have yet to determine whether the opsin immunoreactive perikarya or some other unidentified cell type mediate circadian light detection in the rd/rd retina.
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  • 83
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    Journal of population economics 13 (2000), S. 403-424 
    ISSN: 1432-1475
    Keywords: JEL classification: O41 ; F22 ; Key words: Altruism ; education ; growth ; convergence ; capital mobility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Sociology , Economics
    Notes: Abstract. The aim of this paper is to discuss the process of regional convergence within the framework of an overlapping generations model in which the engine of growth is the accumulation of human capital. In particular, we consider different education funding systems and compare their performance in terms of growth rates and pace of convergence between two heterogeneous regions. The analysis suggests that the choice of a particular education system incorporates a possible trade-off between long run growth rate and short run convergence. In such choice, the initial capital stock and the extent of regional human capital discrepancy appear as central variables.
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  • 84
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    Journal of population economics 9 (1996), S. 415-428 
    ISSN: 1432-1475
    Keywords: H42 ; J 13 ; O 11 ; Fertility ; growth ; public education and health
    Source: Springer Online Journal Archives 1860-2000
    Topics: Sociology , Economics
    Notes: Abstract This paper considers the implications of the financing of government services to children when fertility decisions are endogenously determined. In particular, it is shown that when the services are financed by taxation, the equilibrium outcome is biased away from the socially preferred result. The bias results in higher fertility rates and lower economic growth rates than the efficient social optimum. This arises because each household internalizes the benefits, but not the costs of the tax-financed services. We consider alternative methods of financing the public provision of services and find that a combination of taxation and vouchers can eliminate the bias in the equilibrium outcome.
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  • 85
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    Journal of population economics 11 (1998), S. 273-291 
    ISSN: 1432-1475
    Keywords: JEL classification: F22 ; O3 ; J61 ; Key words: Immigration ; assimilation ; growth ; diversity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Sociology , Economics
    Notes: Abstract. This paper analyzes the welfare effects of immigration and its subsequent effect on ethnic diversity in a model featuring human capital spillovers which depend on the degree of ethnic heterogeneity, variation rates of time preference across individuals and endogenous levels of immigration and assimilation. In the model, an increase in ethnic diversity reduces the spillovers effect for the majority. Nonetheless, immigration can be welfare improving for the majority ethnic group even if it increases the degree of diversity as long as it raises the average human capital level and/or growth rate by increasing the proportion of people with low rates of time preference. However, if an economy is too homogenous, it will not be able to attract immigrants. Finally, if the level of immigration is not too high, then immigration also raises the net benefits to assimilation which leads to a more homogenous economy.
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  • 86
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    Journal of population economics 11 (1998), S. 517-534 
    ISSN: 1432-1475
    Keywords: Key words: Fertility ; mortality ; growth ; JEL classification: J13 ; O41
    Source: Springer Online Journal Archives 1860-2000
    Topics: Sociology , Economics
    Notes: Abstract. Economic and demographic outcomes are determined jointly in a choice-theoretic model of fertility, mortality and capital accumulation. There is an endogenous population of reproductive agents who belong to dynastic families of overlapping generations connected through altruism. In addition to choosing savings and births, parents may reduce (infant) deaths by incurring expenditures on health-care which is also provided by the government. A generalised production technology accounts for long-run endogenous growth with short-run transitional dynamics. The analysis yields testable time series and cross-section implications which accord with the empirical evidence on the relationship between demography and development.
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  • 87
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    Journal of population economics 9 (1996), S. 415-428 
    ISSN: 1432-1475
    Keywords: Key words: Fertility ; growth ; public education and health
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    Topics: Sociology , Economics
    Notes: Abstract. This paper considers the implications of the financing of government services to children when fertility decisions are endogenously determined. In particular, it is shown that when the services are financed by taxation, the equilibrium outcome is biased away from the socially preferred result. The bias results in higher fertility rates and lower economic growth rates than the efficient social optimum. This arises because each household internalizes the benefits, but not the costs of the tax-financed services. We consider alternative methods of financing the public provision of services and find that a combination of taxation and vouchers can eliminate the bias in the equilibrium outcome. JEL classification: H42, J13, O11
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  • 88
    ISSN: 1432-072X
    Keywords: Extracellular proteins ; Surface fibrils ; Algae-fungi-Chrysochromulina ; Immunocytochemistry ; Agglutination ; Fimoriae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An extensive network of extracellular fibrils was revealed by negative staining in the greenish gold algal flagellate, Chrysochromulina breviturrita. These fibrils were of uniform diameter (4–5 nm), sometimes exceeding 5 μm in length. In addition there were short, narrower fibrils (2–3 nm) on the surface of the flagella. Six protein bands were isolated from spent culture medium by SDS-PAGE and one of 80,000 Da was found to polymerize after dialysis into 4–5 nm fibrils identical to those found on the cell surface. Two other proteins of 58,000 Da and 65,000 Da also formed 4–5 nm fibrils but these were either rare or of a shorter length and different appearance. An antiserum directed against the surface 7 nm fibrils (fimbriae) of fungi agglutinated cells of C. breviturrita and some other Prymnesiophyceae and Chrysophyceae, but did not agglutinate cells of algal species in other groups. Immunofluorescence and protein A gold labelling confirmed that antigens related to fungal fimbriae were present on the surface of cells of C. breviturrita. Only the 80,000 and 58,000 Da proteins labelled heavily following protein A gold labelling. Some individual 4–5 nm fibrils labelled with gold were observed in the material prepared from the 80,000 Da band. These results therefore establish that C. breviturrita produces a surface network of fibrils that are serologically related to the fimbriae of fungi, and suggest a previously unrecognized relationship between members of the Prymnesiophyceae, Chrysophyceae and fungal groups.
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  • 89
    ISSN: 1432-072X
    Keywords: Methanobacterium thermoautotrophicum ; Methyl-CoM reductase ; Immunocytochemistry ; Colloidal gold ; Energy conservation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cells of Methanobacterium thermoautotrophicum were fixed with glutaraldehyde, sectioned and labeled with antibodies against the β subunit of component C (=methyl-CoM reductase) of methyl-CoM reductase system and with colloidal gold-labeled protein A. It was found that the gold particles were located predominantly in the vicinity of the cytoplasmic membrane, when the cells were grown under conditions where methyl-CoM reductase was not overproduced. This finding confirms the recent data obtained with Methanococcus voltae showing via the same immunocytochemical localization technique that in this organism methyl-CoM reductase is membrane associated.
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  • 90
    ISSN: 1432-072X
    Keywords: Acidaminococcus fermentans ; Glutamate fermentation ; Electron microscopy ; Immunocytochemistry ; Post-embedding labelling ; Antibody-gold complexes ; Protein A-gold complexes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have investigated the in situ location of glutaconyl-CoA decarboxylase and 2-htdroxyglutaryl-CoA dehydratase in Acidaminococcus fermentans using the antibody-gold and protein A-gold techniques carried out as a post-embedding immunoelectron microscopic procedure. Polyclonal antisera were raised in rabbits against homogeneous fractions of the enzymes. Anaerobically grown cells of A. fermentans of the late exponential growth phase were fixed with 0.2% glutaraldehyde and 0.3% formaldehyde (final concentrations) in the growth medium. Dehydration of the cells was achieved with methanol. The cells were embedded in the low temperature embedding resin Lowicryl K4M. The markers indicative for antigenic sites of the two enzymes unequivocally demonstrate that the sodium pump glutaconyl-CoA decarboxylase is located at the cell periphery being a membrane-bound enzyme as expected whereas 2-hydroxyglutaryl-CoA dehydratase is a soluble cytoplasmic enzyme.
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  • 91
    ISSN: 1432-072X
    Keywords: Key words Protein phosphorylation ; ATP-Pi exchange ; Light-harvesting complexes ; Reaction center ; Photosynthesis ; Photomorphogenesis ; Membrane ; growth ; Polypeptide insertion ; Phospho-amino esters
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Membranes of Rhodobacter capsulatus strain U43 (pTX35) showed qualitatively very similar phosphorylation patterns under in vitro and in vivo conditions. In vitro, it was irrelevant whether the phosphate source was orthophosphate or ATP. Inhibitors of electron transport did not inhibit light-harvesting complex I (LHIα) (B870) polypeptide phosphorylation, except for o-phenanthroline, which was strongly inhibitory. Redox conditions regulated the amount of protein phosphorylated; external redox potentials between +200 and +300 mV promoted the reaction. Phosphorylation was inhibited by uncouplers such as carbonyl cyanide m-chlorophenyl hydrazone and nigericin plus valinomycin plus potassium ions. Inhibitors of the H+-ATPase were also inhibitory when the phosphate source was [32P]Pi or [γ-32P]ATP. From these results, it was concluded that an operative reaction center, a coupled membrane, and external redox potentials higher than +200 mV are required for optimum LHIα phosphorylation. We also demonstrated that phosphorylation of LHIα polypeptide occurs before insertion into the membrane and that phosphate is preferentially incorporated into specific domains within the cytoplasmic membrane. Intracytoplasmic membranes, identified here as light membranes, were found to contain a dephosphorylated LHIα polypeptide.
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  • 92
    ISSN: 1432-072X
    Keywords: Hansenula polymorpha ; Peroxisomes ; Methanol ; Dihydroxyacetone synthase ; Cell fractionation ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The subcellular localization of dihydroxyacetone synthase (DHAS) in the methylotrophic yeast Hansenula polymorpha was studied by various biochemical and immunocytochemical methods. After cell fractionation involving differential and sucrose gradient centrifugation of protoplast homogenates prepared from methanol-grown cells, DHAS cosedimented with the peroxisomal enzymes alcohol oxidase and catalase. Electron microscopy of this fraction showed that it contained mainly intact peroxisomes, whereas SDS-polyacrylamide gel electrophoresis revealed two major protein bands (75 and 78 kDa) which were identified as alcohol oxidase and DHAS, respectively. The localization of DHAS in peroxisomes was further established by immunocytochemistry. After immuno-gold staining carried out on ultrathin sections of methanol-grown H. polymorpha using DHAS-specific antibodies, labelling was confined to the peroxisomal matrix.
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  • 93
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    Archives of microbiology 146 (1987), S. 327-331 
    ISSN: 1432-072X
    Keywords: Actinomycetes ; Nitrogen fixation ; Symbiosis ; Immunocytochemistry ; Ultracryotomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Immunogoldlabelling on ultrathin cryosections of Frankia sp. Cc1.17 showed specific labelling of nitrogenase in the spherical cells called vesicles. No label was found in the hyphae in any cells grown on a medium with combined nitrogen, nor in those to which no specific antiserum was added. Similar results were obtained with cultures grown under high (20%) and low (2%) oxygen tension in the gas phase.
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  • 94
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    Archives of microbiology 157 (1992), S. 218-222 
    ISSN: 1432-072X
    Keywords: Denitrification ; N2O reductase ; Nitrite reductase ; Immunocytochemistry ; Localization ; Two-dimensional electrophoresis ; Cell fractionation ; Pseudomonas stutzeri
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Immunogold labelling techniques on ultrathin sections of low temperature embedded cells yielded evidence for the periplasmic location of the respiratory enzymes N2O reductase and nitrite reductase (cytochrome cd 1) in Pseudomonas stutzeri strain ZoBell. Cell fractionation by spheroplast preparation and two-dimensional electrophoresis showed the absence of a membrane association of these enzymes. Immunocytochemical localization of N2O reductase in a mutant strain deficient in the chromophore of N2O reductase showed the gold label at the cell periphery, indicating that the copper chromophore processing takes place after export of this protein's apoform.
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  • 95
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    Invertebrate neuroscience 1 (1995), S. 199-206 
    ISSN: 1439-1104
    Keywords: phospholipase C ; inositol phosphate ; invertebrate vision ; olfaction ; growth ; differentiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: abstract Phosphatidylinositol-specific phospholipase C (PLC) is a family of enzymes that occupy a pivotal role in one of the largest classes of cellular signaling pathways known. Mammalian PLC enzymes have been divided into four major classes and a variety of subclasses based on their structural characteristics and immunological differences. There have been five invertebrate PLC-encoding genes cloned thus far and these fall within three of the four major classes used in categorizing mammalian PLC. Four of these invertebrate genes have been cloned fromDrosophila melanogaster and one is fromArtemia, a brine shrimp. Structural characteristics of the invertebrate enzymes include the presence of highly conserved Box X and Box Y domains found in major types of mammalian PLC as well as novel features. Two of the invertebrate PLC genes encode multiple splice-variant subtypes which is a newly emerging level of diversity observed in mammalian enzymes. Studies of the invertebrate PLCs have contributed to the identification of the physiological functions of individual isozymes. These identified roles include cellular processes such as phototransduction, olfaction, cell growth and differentiation.
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  • 96
    ISSN: 1440-1703
    Keywords: aquatic macrophyte ; biomass ; growth ; leaf life span ; shoot density ; spatial distribution ; Zizania latifolia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The population and production ecology of aZizania latifolia stand at a sheltered shore of the Hitachi-Tone River were investigated. Shoot emergence was observed twice a year; the fist was a synchronized shoot emergence in April and the second was from August to October. Aboveground biomass was mostly occupied by leaves and peaked at 1500 g dry weight m−2 in August. The belowground biomass also reached its peak, 750 g dry weight m−2, in August. The secondary shoots were small in spite of their high density. Leaves were produced continuously throughout the season. The leaf life span was as short as 55.6 days for cohorts that emerged from May through to September. Total annual net production ofZ. latifolia could be more than 3400 g dry weight m−2. Shoot clusters of several centimeters were observed in April. The following self-thinning caused a regular distribution of the remaining shoots in August. Most shoots produced in August to October were found near a shoot persisting since April. They showed more concentrated distribution than shoots in April. A large biomass allocation to leaves and the ability to produce many clump shoots during the late growing period may facilitate dominance ofZ. latifolia in relatively sheltered sites.
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    Ecological research 15 (2000), S. 101-106 
    ISSN: 1440-1703
    Keywords: comparative ecology ; growth ; marine fish ; patterns ; reproduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: A number of strong regularities characterize certain very basic biological parameters in marine fishes. For example, the ovulated eggs of fish usually measure approximately 1 mm in diameter. The small, relatively uniform size of the eggs means that almost all fish larvae experience environmental variability at very similar scales, which itself establishes strong constraints for, and links between reproduction and recruitment. Additional constraints emerge from seawater being a poor medium for respiration, which establishes further linkages between growth and mortality. These constraints have produced strongly convergent features, and thence the patterns in reproduction and growth of marine fishes that are presented.
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    Plasma chemistry and plasma processing 11 (1991), S. 371-386 
    ISSN: 1572-8986
    Keywords: Plasmas ; aerosol ; modelling ; growth ; reactor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Technology
    Notes: Abstract A model is proposed for the analysis of the production of ultrafine particles in thermal plasma reactors. The model initially solves the fluid flow, temperature, and concentration fields using a classical control volume approach. The nucleation and growth of ultra fine particles are then solved along each streamline. The evolution of the particle distribution is described by a statistical approach, using the first moments of the distribution as the dependent variables. Brownian coalescence is considered in the free molecular regime. In the discussion, the model is used to demonstrate the effects of some important parameters, such as the initial concentration of metal vapor, its radial distribution, and the radial injection of a cooling gas, on the particle size distribution.
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    Journal of thermal analysis and calorimetry 52 (1998), S. 537-542 
    ISSN: 1572-8943
    Keywords: fractals ; growth ; nucleation
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    Topics: Chemistry and Pharmacology
    Notes: Abstract The author presents a model based on multi-step nucleation for the heterogeneous decompositions described by nucleation and growth phenomena, taking into account the fractal nature of the nuclei.
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    Aquaculture international 6 (1998), S. 1-17 
    ISSN: 1573-143X
    Keywords: Atlantic salmon (Salmo salar) ; Condition factor ; feeding regimes ; growth ; proximate composition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effects of different feeding methods on growth and slaughter traits of Atlantic salmon, Salmo salar, have been examined. Swimming activity associated with feeding was greatest amongst fish fed using automatic feeders, intermediate amongst those fed predetermined rations by hand, and least in groups of fish fed to satiation twice per day. Fish fed to satiation ate more and grew faster than did those provided with rations predicted to support high rates of growth, but the fish fed to satiation had higher feed:gain ratios than those given predetermined rations as two meals per day. The muscle tissues of the fish fed to satiation had higher lipid (%) and lower moisture (%) contents than did those of the fish fed the predetermined rations, but differences in muscle composition could be accounted for by differences in the size of the fish. Lipid was positively, and moisture negatively, correlated with fish size, whereas per cent protein and dress-out losses were little influenced by fish size and feeding regime. The fish fed to satiation were slightly heavier for a given length than those in the other groups. There was, however, no evidence that the largest fish had the highest condition factor, nor could condition factor be used to predict per cent lipid in the muscle tissue. Size variation increased in all groups of fish, and this was, in part, due to the cessation of growth of maturing individuals. Size variation was, however, most marked in the groups fed using automatic feeders, suggesting that interindividual competition for food was greatest amongst these fish.
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