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  • Immunocytochemistry  (856)
  • Drosophila  (737)
  • growth  (678)
  • Springer  (2.271)
  • 1
    ISSN: 1436-6215
    Schlagwort(e): purinreiche Nahrung ; Wachstum ; Metaboliten ; Harnsäuretransport ; Hund ; purine-rich diet ; growth ; metabolites ; uric acid transport ; dog
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft , Medizin
    Beschreibung / Inhaltsverzeichnis: Summary In a nutritional tolerance study 36 young dogs were fed over 52 weeks high or low purine diets at an average paired feeding intake of 0.6 and 80 mg purine-N/MJ per day. The high purine diet resulted in a significant decrease of growth rate by −23 % (Beagles) and −38 % (Dalmatians) and of feed efficiency (−29 and −42 %). The fasting levels of allantoin, uric acid and uracil in blood plasma were significantly increased. During the experiment a metabolic adaptation to the high purine diet decreased the plasma concentrations of uric acid, uracil and in part of allantoin. The high purine diet effected a significant increase of Km (2.5-fold) and of Vmax (1.6-fold) of uric transport through the erythrocyte membrane. The results documented disadvantageous effects of high purine nutrition during juvenile development.
    Notizen: Zusammenfassung In einer Verträglichkeitsstudie erhielten 36 junge Hunde über 52 Wochen eine purinreiche oder purinarme Diät mit durchschnittlich 6 bzw. 80 mg Purin-N/MJ pro Tag bei gruppengleicher Aufnahme („paired feeding“). Die Hochpurin-Diät führte zu einer signifikanten Verminderung der Gewichtsentwicklung um −23% (Beagles) bzw. −38% (Dalmatiner) und der Futterverwertung (−29 bzw. −42%) sowie zu einer signifikanten Erhöhung der Nüchternwerte von Allantoin, Harnsäure und Uracil im Blutplasma. Während der Versuchsdauer machte sich eine metabolische Adaptation an die Hochpurin-Diät mit signifikanter Erniedrigung von Harnsäure, Uracil und teils auch Allantoin im Plasma bemerkbar. Die Hochpurin-Diät bewirkte eine signifikante Zunahme der Km (2,6fach) und Vmax (1,6fach) des Harnsäuretransports durch die Erythrozytenmembran. Die Ergebnisse belegen nachteilige Effekte purinreicher Ernährung während der Jugendentwicklung.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    European journal of nutrition 36 (1997), S. 299-302 
    ISSN: 1436-6215
    Schlagwort(e): Energy retention ; protein retention ; fat retention ; growth ; body composition ; broilers ; Energieansatz ; Proteinansatz ; Fettansatz ; Wachstum ; Körperzusammensetzung ; Broiler
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft , Medizin
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Mit steigender Aufnahme erhöht sich der Körperfettgehalt und der Körperproteingehalt nimmt ab. Es wird oft angenommen, daß jede Zunahme im Ansatz mit mehr Fett und weniger Protein verbunden ist. Experimentelle Ergebnisse widerlegen jedoch diese Annahme. In zwei Experimenten mit männlichen Broilerhühnern wurden die Tiere auf einem Niveau von 60 % und 100 % der empfohlenen Energieaufnahme gefüttert. Die Körperzusammensetzung bei 1500 g zeigte, wie erwartet, daß sich bei steigenden Aufnahmen der Körperfettgchalt vergrößert und der Körperproteingehalt abnimmt. Fettund Proteinansatz waren linear mit der totalen Energieretention (ER) korreliert. Das bedeutet, daß jede Zunahme im Ansatz den gleichen Protein- und Fettgehalt besitzt. Wenn der Fettansatz gleich Null ist wird nur Protein, etwa 50 % des maximalen Ansatzes, retiniert. Wenn ER=O ist, wird Protein angesetzt und Fett mobilisiert. Energie-und N-Bilanzuntersuchungen bestätigen die konstante Zusammensetzung jeder Vergrößerung des Ansatzes. Die Ergebnisse beider Experimente zeigen, daß die ER aus zwei Komponenten besteht: einem basalen konstanten täglichen Proteinansatz und einer variablen zusätzlichen ER, die hauptsächlich aus Fett besteht. Der basale Proteinansatz beträgt etwa 50 % des maximalen Ansatzes. Mit steigenden Energieaufnahmen wird der basale Proteinansatz mit einer zusätzlichen Menge von Protein und Fett im konstanten Verhältnis ergänzt.
    Notizen: Summary With increasing intakes the body fat content increases and that of protein decreases. It is most often assumed that this is brought about because each increment in retention contains more fat and less protein. Experimental results, however, showed that this explanation is not true. In two experiments male broiler chickens were fed at levels between 60 and 100 % of recommended energy intake. Body composition at 1500 g showed, as expected, that with increasing intakes body fat content increased and protein content decreased. Both fat and protein retention per day were linearly related to total energy retention (ER). This means that each increment in retention has the same protein and fat content. At zero fat retention only protein was retained, about 50 % of maximal retention. At zero ER protein was retained and fat mobilized. Energy and N balance experiments confirmed the constant composition of each increment in retention. The results of both experiments show that total ER consisted of two components: a basic constant daily protein retention and a variable additional ER, mainly consisting of fat. The basic protein retention is about half of maximal retention. With increasing energy intakes the basic protein retention is combined with an additional amount of protein and fat in a constant ratio.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    European journal of nutrition 36 (1997), S. 332-335 
    ISSN: 1436-6215
    Schlagwort(e): Nutrient intake ; protein metabolism ; protein synthesis ; growth ; energy expenditures ; Nährstoffaufnahme ; Proteinstoffwechsel ; Proteinsynthese ; Wachstum ; Energieaufwand
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft , Medizin
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Eine Zunahme von fettfreiem Gewebe tritt auf, wenn die Proteinsynthese größer ist als der Proteinabbau. Obwohl während des Wachstums von der Geburt bis zur Reife die absoluten Proteinsynthese- und -abbauraten ansteigen, nehmen dagegen die fraktionellen Raten ab. Bcide Prozesse reagieren auf die Nährstoffaufnahme. Es gibt aber deutliche Unterschiede zwischen den verschiedenen Geweben. Protein, Kohlenhydrate und Fett können den Proteinansatz bei unreifen Tieren und Kindern stimulieren. Die zugrundeliegenden Mechanismen und die Energieaufwendungen scheinen jedoch unterschiedlich zu sein.
    Notizen: Summary Lean tissue growth occurs when the rate of protein synthesis exceeds the rate of protein breakdown. Althoughabsolute rates of protein synthesis and breakdown rise during growth from birth to maturityfractional rates fall. Both these processes are sensitive to nutrient intake but responses to feeding vary greatly amongst different tissues. Protein, carbohydrate and fat can all stimulate body protein accretion in immature animals and in children but the mechanisms by which they do so, and the energy expenditures involved, seem to be different.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    Empirical economics 24 (1999), S. 23-44 
    ISSN: 1435-8921
    Schlagwort(e): Key words: Cointegration ; convergence ; growth ; Kalman filter ; JEL classifications: C22 ; O47 ; O57
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Wirtschaftswissenschaften
    Notizen: Abstract. Augmented Dickey Fuller (ADF) and Kalman filter convergence tests are applied to annual GDPs per head to 16 industrialised countries from 1890 to 1989. Results favour convergence towards the US with a structural break following the Second World War. Estimates suggest that steady-states were higher after the war and that speeds of convergence are different across countries. The Kalman filter method dismissed the no convergence hypothesis more often than its ADF counterpart. This could explain the apparent contradiction in earlier empirical work on similar data sets (cross-section methods tended to favour convergence while time series methods were unable to dismiss the no convergence hypothesis.)
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    ISSN: 1570-7458
    Schlagwort(e): Vitis vinifera ; Empoasca vitis ; leafhopper ; photosynthesis ; transpiration ; stomatal conductance ; mesophyll conductance ; growth ; yield ; fruit quality ; starch ; carbohydrate reserves
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The impact of the grape leafhopper,Empoasca vitis, on leaf gas exchange, plant growth, yield, fruit quality and carbohydrate reserves of the grapevines,Vitis vinifera L., was studied. Gas exchange was measured on the discolored (red) and the green parts of infested main leaves and on leaves from uninfested vines. Photosynthesis and mesophyll conductance were severely reduced on main leaves showing leafhopper feeding symptoms. The stomatal conductance of the red leaf section of infested main leaves was lower than on undamaged control leaves. Additionally, the red leaf section of infested main leaves showed lower transpiration rates when compared to the green parts of the same leaves and to undamaged control leaves. Gas exchange processes of lateral leaves were not affected by leafhopper feeding. Leafhopperload on main leaves was correlated to visual damage symptoms. At 71.8 leafhopper-days per leaf up to 40% of the main leaf area of the infested plants was discolored from the borders towards the center. Lateral leaves showed no feeding symptoms. Shoot diameter, pruning weight and carbohydrate reserves in the wood were not affected by leafhoppers. Lateral leaf area growth was significantly stimulated on plants infested by leafhoppers. No decrease in yield and fruit quality with leafhopper-loads up to 71.8 leafhopper-days per leaf were observed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    Digitale Medien
    Digitale Medien
    Springer
    Entomologia experimentalis et applicata 80 (1996), S. 141-144 
    ISSN: 1570-7458
    Schlagwort(e): Polygonia c-album ; Nymphalidae ; host-plant selection ; growth ; size
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    ISSN: 1570-7458
    Schlagwort(e): amylase inhibitor ; red kidney bean ; hard red winter wheat ; growth ; insects ; beetles ; plant resistance ; stored products ; protease inhibitor
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Insect α-amylase inhibiting and/or growth inhibiting activities of proteinaceous inhibitors from red kidney bean (Phaseolus vulgaris) and hard red winter wheat (Triticum aestivum) were examined. The bean inhibitor was most effectivein vitro against α-amylases from the red flour beetle (Tribolium castaneum) and the confused flour beetle (T. confusum), followed by those from the rice weevil (Sitophilus oryzae) and yellow mealworm (Tenebrio molitor). The insect enzymes were from two- to 50-fold more susceptible than human salivary α-amylase. When the inhibitors were added at a 1% level to a wheat flour plus germ diet, the growth of red flour beetle larvae was slowed relative to that of the control group of larvae, with the bean inhibitor being more effective than the wheat inhibitor. Development of both the red flour beetle and flat grain beetle (Cryptolestes pusillus) was delayed by 1% bean inhibitor, but development of the sawtoothed grain beetle (Oryzaephilus surinamensis) and lesser grain borer (Rhyzopertha dominica) was not affected by either the bean or wheat inhibitor at the 1% level. Rice weevil adults fed a diet containing 1% bean or wheat inhibitor exhibited more mortality than weevils fed the control diet. When the wheat amylase inhibitor was combined with a cysteine protease inhibitor, E-64, and fed to red flour beetle larvae, a reduction in the growth rate and an increase in the time required for adult eclosion occurred relative to larvae fed either of the inhibitors separately. The bean inhibitor was just as effective alone as when it was combined with the protease inhibitor. These results demonstrate that plant inhibitors of insect digestive enzymes act as growth inhibitors of insects and possibly as plant defense proteins, and open the way to the use of the genes of these inhibitors for genetically improving the resistance of cereals to storage pests.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    Digitale Medien
    Digitale Medien
    Springer
    Entomologia experimentalis et applicata 76 (1995), S. 25-35 
    ISSN: 1570-7458
    Schlagwort(e): dispersal ; flight duration ; cactophilic ; Drosophila ; age effects ; body size
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The flight ability ofDrosophila aldrichi (Patterson & Crow) andD. buzzatii (Patterson & Wheeler) using tethered flights, was measured with respect to age-related changes, genetic variation and adult body size variation induced by rearing at different larval densities.Drosophila buzzatii flew for much longer thanD. aldrichi, especially females, but age-related changes in flight duration were significant only forD. aldrichi. Effects of body size on flight ability were significant inD. buzzatii, but not inD. aldrichi. InD. buzzatii, there was a significant genotype-environment interaction (larval density × line) for flight duration, with short and average flight duration isofemale lines showing longer flights, but a long flight duration line shorter flights as body size decreased (i.e., as larval density increased). Heritability estimates for flight duration were similar in the two species, but flight duration showed no significant genetic correlations with developmental time, body size or wing dimensions (except for one wing dimension inD. buzzatii). Although not significantly different between the species, heritabilities for life-history traits (adult size and developmental time) showed contrasting patterns — with higher heritability for body size (body weight and thorax length) inD. buzzatii, and higher for developmental time inD. aldrichi. In agreement with limited previous field evidence,D. buzzatii is better adapted for colonization than isD. aldrichi.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 9
    Digitale Medien
    Digitale Medien
    Springer
    Entomologia experimentalis et applicata 90 (1999), S. 175-181 
    ISSN: 1570-7458
    Schlagwort(e): Olfactory response ; Drosophila ; menthol ; bioassay ; trap assay
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A modification of the trap assay (Woodard et al., 1989) was used to evaluate the response of Drosophila melanogaster (Meigen) to food media containing menthol. Dose-response curves for flies to mentholic foods were produced for flies that had been pre-exposed to menthol, during development and adult life, and flies that had not been exposed to menthol before the assay. Mentholic food media were less attractive to Drosophila than plain food medium. Rearing flies on a medium containing menthol reduced their aversion to some concentrations of menthol. The rearing effect was not simply due to lowered general activity levels resulting from developing in a medium containing menthol. There was a threshold concentration of menthol in the rearing medium below which we found no induced behavioural change.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 10
    Digitale Medien
    Digitale Medien
    Springer
    Entomologia experimentalis et applicata 95 (2000), S. 97-103 
    ISSN: 1570-7458
    Schlagwort(e): butterfly ; Formicidae ; food ; growth ; mutualism ; myrmecophily ; parasitism
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Caterpillars of the lycaenid butterfly Maculinea rebeli Hirschke (Lepidoptera: Lycaenidae) live for 11–23 months as social parasites in Myrmica (Hymenoptera: Formicidae) red ant nests, a trait that is believed to have evolved from mutualistic myrmecophilous ancestry. Although Maculinea rebeli caterpillars harm Myrmica larvae, they simultaneously produce copious secretions which the adult worker ants imbibe, perhaps representing a vestige of the ancestral mutualism. We report the results of laboratory experiments designed to test alternative hypotheses: (i) Maculinea rebeli caterpillars provide a beneficial source of sugar in return for being tended by Myrmicaworkers; (ii) Maculinea rebeli harms its host by stressing the workers by competing for available sugar. Comparisons were made of Myrmica worker fitness after 90–450 days under all possible combinations of three experimental treatments: ± M. rebeli caterpillars, ± sucrose and ± ant brood. Caterpillars always reduced the survival of both ant workers and their larvae, even when sugar was not provided, suggesting that M. rebeli is wholly parasitic on all stages in its host colony. The results also confirmed the importance of sucrose in the diet of Myrmica, and showed that M. rebeli caterpillars which eat ant brood to supplement their normal trophallactic feeding by workers develop more quickly - but have the same survival and pupal weights – as caterpillars that are fed solely by worker ants.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 11
    Digitale Medien
    Digitale Medien
    Springer
    Entomologia experimentalis et applicata 79 (1996), S. 335-344 
    ISSN: 1570-7458
    Schlagwort(e): alkaloid ; developmental time ; early instars ; growth ; Manduca sexta ; molting duration ; phenolics ; plant-herbivore interactions ; thermal regime
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract To understand the mechanisms underlying plant-insect herbivore interactions, it is necessary to examine the simultaneous effects of temperature, food quality and larval age. We examined the simultaneous effects of three allelochemicals (tomatine, rutin and chlorogenic acid) on the performance of first and second instar Manduca sexta larvae under two representative thermal regimes 21 : 10°C and 26 : 15°C for spring and summer, respectively. Thermal regime and allelochemicals interacted to influence the time from egg hatch to ecdysis to the third instar. On average, it took about half as much time to reach the third instar at 26 : 15°C as it did at 21 : 10°C. Separately, tomatine and rutin had a negative effect on developmental time from egg hatch to the third instar, but their simutaneous effects were not additive. Chlorogenic acid significantly reduced the negative effect of tomatine. The magnitude of the allelochemical effect was larger at the cooler thermal regime compared to the warmer regime. For instance, chlorogenic acid by itself had no effect at the 26 : 15°C regime, but at the 21 : 10°C regime it significantly shortened total developmental time. The effect of chlorogenic acid on stadium duration was distinctly different for the two instars. Chlorogenic acid shortened stadium duration of first instar larvae. However, depending on thermal regime and the presence of tomatine, chlorogenic acid had a negative, positive or neutral effect on stadium duration of second instar larvae. Molting duration of second instar larvae was shortened by a half day at the warmer thermal regime but was not affected by the allelochemicals. Final larval weight was influenced by rutin and chlorogenic acid. Caterpillars fed diets containing 20 μmoles of rutin were on average 10% lighter than those fed plain diet, whereas those fed diets containing 20 μmoles of chlorogenic adic were on average 7% heavier. However, the effect of chlorogenic acid depended on thermal regime. Overall, our results indicated that: 1) temperature and food quality can interact to influence insect performance and 2) these effects are influenced by larval age.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 12
    Digitale Medien
    Digitale Medien
    Springer
    Entomologia experimentalis et applicata 86 (1998), S. 13-24 
    ISSN: 1570-7458
    Schlagwort(e): Drosophila ; cytoplasmic incompatibility ; Wolbachia ; temperature ; antibiotics ; density
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The effects of high temperatures, antibiotics, nutrition and larval density on cytoplasmic incompatibility caused by a Wolbachia infection were investigated in Drosophila simulans. Exposure of larvae from an infected stock to moderate doses of tetracycline led to complete incompatibility when treated females were crossed to infected males; the same doses only caused a partial restoration of compatibility when treated males were crossed to uninfected females. In crosses with treated females, there was a strong correlation between dose effects on hatch rates and infection levels in embryos produced by these females. Ageing and rearing males at a high temperature led to increased compatibility. However, exposing infected females to a high temperature did not influence their compatibility with infected males. Male temperature effects depended on conditions experienced at the larval stage but not the pupal stage. Exposure to 25 °C reduced the density of Wolbachia in embryos compared with a 19 °C treatment. Low levels of nutrition led to increased compatibility, but no effect of larval crowding was detected. These findings show the ways environmental factors can influence the expression of cytoplasmic incompatibility and suggest that environmental effects may be mediated by bacterial density.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 13
    Digitale Medien
    Digitale Medien
    Springer
    Entomologia experimentalis et applicata 72 (1994), S. 115-123 
    ISSN: 1570-7458
    Schlagwort(e): ecdysteroid agonists ; juvenile hormone analogue ; Spodoptera exigua ; growth ; moulting ; metamorphosis ; imaginal discs
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Biological activity assays with RH 5849 and RH 5992 indicated that both compounds affected growth and development of last-instar larvae ofSpodoptera exigua (Hübner) (Lepidoptera: Noctuidae) in a dose-dependent manner. Within the first 24 h after treatment by continuously offering leaves dipped in a water solution of ≥50 mg/l RH 5849 and ≥0.5 mg/l RH 5992, symptoms of a prematurely induced larval moult and head capsule apolysis were visible. Intoxicated larvae died shortly afterwards, showing signs of unsuccessful ecdysis. LC50-values of RH 5849 and RH 5992 for fifth-instarS. exigua larvae were 110 and 2.5 mg/l, respectively. Pyriproxyfen alone affected the larval stage and disturbed normal metamorphosis. One supernumerary larval instar occurred occasionally. LC50-value for pyriproxyfen was 1.7 mg/l. Larvae simultaneously treated with RH 5849 or RH 5992 and pyriproxyfen, continued to grow until they attained a size and weight about 2–3 times that of the controls. This growth was accompanied by at least one and sometimes two supernumerary moults. Concerning thein vivo imaginal wing disc growth and development, only in larvae treated with 10 and 50 mg/l RH 5849 or 0.5 mg/l RH 5992, tracheole migration was observed earlier than in the controls. When applying 300 mg/l RH 5849 or 3–7 mg/l RH 5992, the discs remained small and no signs of tracheole migration were observed. In larvae simultaneously treated with RH 5849 or RH 5992 and pyriproxyfen, tracheole migration was not prematurely induced and a pupal cuticle was produced in the discs of larvae, undergoing a supernumerary moult. No clear signs of evagination were observed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 14
    Digitale Medien
    Digitale Medien
    Springer
    Entomologia experimentalis et applicata 80 (1996), S. 443-451 
    ISSN: 1570-7458
    Schlagwort(e): beta-carboline alkaloid ; Trichoplusia ni ; harmane ; harmaline ; amitriptyline ; imipramine ; monoamine oxidase inhibitor ; tricyclics ; growth ; feeding behavior
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract In this study we investigated the effects of two naturally occurring beta-carboline alkaloids and two synthetic tricyclic antidepressants on the growth and food consumption of fifth instar larvae of the cabbage looper, Trichoplusia ni Hübner (Lepidoptera: Noctuidae). In artificial diets at high concentrations (3,000 ppm), harmane, amitriptyline, and imipramine reduce growth and feeding; harmane reduced feeding consistently at a lower concentration (200 ppm). In animals other than insects, beta-carboline alkaloids inhibit monoamine oxidase (MAO) activity and thus affect rates of disposition of serotonin and other monoamine neurotransmitters. Because brain serotonin levels are associated with variation in rates of carbohydrate and protein intake in insects, the effects of beta-carboline alkaloid ingestion on dietary self-selection behavior were examined. Choosing between diets lacking carbohydrate but containing protein and diets lacking protein but containing carbohydrate, larvae consumed a greater proportion of diet containing protein but lacking carbohydrate in the presence of harmane than in its absence. These results are consistent with beta-carboline alkaloid-mediated persistence of serotonin in the brain due to MAO inhibition. Alternatively, these results could reflect alkaloid-mediated peripheral inhibition of sucrose taste receptors influencing ingestive behaviors. That beta-carboline alkaloid ingestion is associated with changes in feeding behavior is consistent with a possible defensive role for these compounds in plant foliage.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 15
    ISSN: 1570-7458
    Schlagwort(e): insect endocrinology ; ecdysone ; juvenile hormone ; reproduction ; growth ; inhibin ; oostatin
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The first insect folliculostatic peptide was isolated from vitellogenic ovaries of the mosquitoAedes aegypti. This decapeptide directly inhibits trypsin biosynthesis in the gut, and indirectly ovarian development. The factor was named Trypsin Modulating Oostatic Factor or TMOF by its discoverers. From the fleshfly Neobellieria bullata 2 folliculostatins have been isolated, the hexapeptide Neb-TMOF and the 19-mer Neb-colloostatin. The available data suggest that at least 2 of the 3 folliculostatins originate from matrix (like) proteins present in the ovary, a hitherto unknown source of hormones. Furthermore, one of the folliculostatins (Neb-TMOF) is a potent inhibitor of ecdysone biosynthesis by larval ring glands of fleshflies. The discovery of the dipteran folliculostatins, which do not show any resemblance to inhibins of vertebrates, may significantly contribute to a better understanding of the hormonal control of growth in insects and perhaps, in other animals as well. None of the 3 folliculostatins is blocked at its N- or C-terminus. This, in combination with the pleiotropy of their effects and the narrow species specificity make such peptides prime candidates for, testing their potential in insect pest control by means of molecular biological methods.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 16
    ISSN: 1570-7458
    Schlagwort(e): Chrysoperla carnea ; food consumption ; growth ; development ; efficiency of food utilization
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Larvae of the common green lacewing,Chrysoperla carnea (Stephens)(Neuroptera: Chrysopidae), were fed either an optimal or a suboptimal number of eggs of the mothAnagasta kuehniella (Zeller) in the first and/or second larval instar, or in all three larval instars. Parameters for the duration, growth, and the efficiency of conversion of ingested food to body substance (ECI) of each instar were established for seven different dietary regimes. Larvae that had a suboptimal food supply in the first instar had a significantly longer developmental time, gained significantly less weight, and had a slightly lower ECI in that instar larvae. Suboptimally-fed second less, but remained only slightly longer in that instar and had a similar ECI to optimally-fed second instar larvae. The developmental time of suboptimally-fed third instar larvae was similar to that of optimally-fed larvae of that stage. Whereas the growth of the former was significantly less than that of larvae optimally fed in that instar, the ECI of the former was significantly higher. Despite the relatively smaller size of larvae fed suboptimally in the first and/or second instar, when such larvae were subsequently supplied with an overabundance of prey eggs, they consumed approximately the same number of eggs during the remainder of their larval life as did larvae whose food supply had not been restricted previously. When larvae were allowed to consume different numbers of eggs in their third instar, their gain in weight and therefore the weights attained by the resulting adults (based on the weights of 3-day-old cocoons) had a highly significant positive correlation with the number of eggs consumed in this instar.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 17
    Digitale Medien
    Digitale Medien
    Springer
    Entomologia experimentalis et applicata 67 (1993), S. 135-142 
    ISSN: 1570-7458
    Schlagwort(e): nutrition ; utilization ; efficiency ; growth ; metabolism ; energy ; respirometry ; Pieris
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Conventional gravimetry and a combination of gravimetry and respirometry were compared for their precision in measuring respiration and metabolic efficiency of growth of final stadiumPieris brassicae L. (Pieridae, Lepidoptera) caterpillars. This was done both for caterpillars feeding on an artificial diet and for caterpillars feeding on excised leaf material of a host plant,Brassica oleracea L. Gravimetry produced significantly greater variation in the total amount of matter respired and the metabolic efficiency than indirect calorimetry for caterpillars feeding on plant material, while the two methods gave similar results for the caterpillars reared on a meridic artificial diet. Respirometry (indirect calorimetry) revealed that caterpillars feeding on the artificial diet were growing with a higher metabolic efficiency than caterpillars feeding on the host plant. This difference was not revealed by conventional gravimetry. It is argued that metabolic efficiencies as derived from gravimetric budget calculations are subject to a number of random errors that distort precise determination of metabolic efficiencies in studies involving plant food.
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  • 18
    Digitale Medien
    Digitale Medien
    Springer
    Entomologia experimentalis et applicata 67 (1993), S. 233-239 
    ISSN: 1570-7458
    Schlagwort(e): inbreeding ; colonization ; isofemale line ; Drosophila ; Diptera ; Leptopilina boulardi ; Cynipidae ; Hymenoptera
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Beschreibung / Inhaltsverzeichnis: Résumé D. melanogaster (Meigen) a été utilisé pour tester la capacité des lignées isofemelles à conserver la variabilité génétique d'une population naturelle. Deux types d'expériences ont été réalisées. L'une a consisté à déterminer la variabilité génétique de 3 locus enzymatiques pour 32 lignées isofemelles à la première et à la 23ème génération d'élevage au laboratoire. L'autre a consisté à tester la capacité des larves à éliminer un parasitoïde par le processus d'encapsulation après 8 années d'élevage au laboratoire. D'une façon générale, certaines lignées isofemelles perdent de la variabilité durant les 23 générations de l'étude. Mais la fréquence globale des allèles reste inchangée si l'on considère l'ensemble des 32 lignées. Le seul allèle rare observé a également été conservé. Les modifications des fréquences allèliques à chacun des locus ont lieu de façon indépendante les unes des autres. La variabilité génétique d'un caractère biologique, la capacité des larves à encapsuler le parasitoïde, a également varié, mais elle a pu être restaurée à un niveau proche de la population initiale en rassemblant plusieurs individus de chacune des lignées.
    Notizen: Abstract Drosophila melanogaster (Meigen) was used to test the power of isofemale lines in preserving genetic variability. We performed experiments in two ways. One series consisted of measuring the genetic variability for three enzymatic loci in 32 isofemale lines, in the first and 23rd generations of culture. In the second series, we tested the capacity of the larvae to eliminate a parasitoid by encapsulation after eight years of laboratory breeding. In general, individual isofemale lines appeared to change during the 23 generations of the study, but the global frequency of these alleles among the 32 isofemale lines stayed relatively unchanged. The only rare allele observed was also conserved. Changes in allozyme frequencies at any one locus were independent of those at other loci. Genetic variation of a biological trait, the capacity of the larvae to encapsulate a parasitoid, also changed, but it could be restored to a level close to that of the starting population by mass hybridizing together individuals of each line.
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  • 19
    Digitale Medien
    Digitale Medien
    Springer
    Entomologia experimentalis et applicata 63 (1992), S. 47-54 
    ISSN: 1570-7458
    Schlagwort(e): Azadirachtin ; Manduca sexta ; growth ; feeding efficiency ; nitrogen utilisation ; proteinase ; trypsin ; allelochemicals ; antifeedant ; herbivory
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract When given by injection to tobacco hornworm caterpillars, Manduca sexta, the allelochemical azadirachtin inhibits growth without reducing food intake. The growth reducing effect of azadirachtin is therefore in this case independent of the compound's well-known antifeedant effect. The cause of this reduced rate of growth is an increase in the costs associated with growth. These increased costs are largely a consequence of a decrease in the efficiency of utilisation of dietary nitrogen. This is associated with a drastic reduction in the activity of midgut trypsin. Azadirachtin has no effect on the activity of trypsin in vitro. Thus azadirachtin directly or indirectly inhibits the production of trypsin by the enzyme-secreting cells of the midgut wall; it is suggested that this is the cause of the increased costs and reduced rate of growth. The interesting parallel between this plant defence strategy and that of direct inhibition of herbivore proteinases by allelochemical proteinase inhibitors is discussed.
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  • 20
    Digitale Medien
    Digitale Medien
    Springer
    Entomologia experimentalis et applicata 53 (1989), S. 257-265 
    ISSN: 1570-7458
    Schlagwort(e): Antifeedant ; insecticide ; bisabolangelone ; analog ; feeding ; growth ; molting ; mortality ; sesquiterpenoid ; development ; phago-stimulant ; Pieris brassicae L. ; Lepidoptera ; Pieridae ; Angelica silvestris L. ; Umbelliferae
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Bisabolangelone und drei Analoge wurden unter Laboratoriumsbedingungen auf ihre frasshemmende Wirkung in Raupen von Pieris brassicae L. (Lepidoptera: Pieridae) geprüft. Die Resultate von Zweifach-Wahlversuchen zeigten, dass sowohl die frasshemmende als auch die insektizide Wirkung von Bisabolangelone in den Analogen stark reduziert ist oder ganz verloren ging. Weitere Untersuchungen mit niedrigen Bisabolangelone-Dosen zeigten, dass das Fehlen von wohlschmeckender Nahrung zu einer drastischen Reduktion der Nahrungsaufnahme und des Wachstums der Raupen sowie zur Verhinderung der Häutung und zu hoher Mortalität führt. Deformationen, wie sie von bisabolangelone-behandelten Vorratsschädlingen gemeldet wurden, konnten bei P. brassicae nicht beobachtet werden.
    Notizen: Abstract Bisabolangelone and three analogs were assayed, under laboratory conditions, for their antifeedant activity against Pieris brassicae L. (Lepidoptera: Pieridae) larvae. The results of dual-choice studies revealed that the potent antifeedant activity of bisabolangelone is greatly reduced or lost in the analogs. Insecticidal activity observed in the former was not detected either. Further studies using the lower rates of bisabolangelone showed that the absence of a preferable food resulted in a drastic reduction in feeding and growth, and produced high mortality and inhibition of molting. No developmental deformities reported in stored product insects treated with bisabolangelone were observed in P. brassicae during our studies.
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  • 21
    Digitale Medien
    Digitale Medien
    Springer
    Entomologia experimentalis et applicata 94 (2000), S. 159-171 
    ISSN: 1570-7458
    Schlagwort(e): Drosophila ; induction ; habituation ; associative learning ; T-maze olfactometer
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Experiments reported in this paper investigate the properties of a change in the responsiveness of adult Drosophila melanogaster induced by exposure to different rearing media. This effect has previously been described as habituation or associative learning. Exposure to food medium containing 0.08% menthol induced a positive response to menthol odour in a T-maze olfactometer. A brief (one hour) exposure to mentholic food just before testing was sufficient to induce a change in responsiveness. The effect did not persist through periods of more than an hour of separation from mentholic medium. Effects induced by exposure to a single compound were not specific to that compound alone. Menthol-reared flies (MRFs) differed from plain reared flies (PRFs) in their responsiveness to the odours of benzaldehyde and ethyl acetate, as well as menthol, and exposure to ethyl acetate induced a change in response to menthol odour. That there was an induced positive response to menthol in MRFs suggests that conventional habituation is insufficient to explain the induced change in responsiveness, but the generalised nature of this behavioural induction in MRFs is hard to explain in terms of associative learning. The mechanism underlying the induction remains elusive.
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  • 22
    Digitale Medien
    Digitale Medien
    Springer
    Journal of insect behavior 11 (1998), S. 691-712 
    ISSN: 1572-8889
    Schlagwort(e): cactus ; Drosophila ; geographic variation ; host preference behavior ; Sonoran Desert ; volatiles
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Previous studies have suggested that all populations of cactophilic Drosophila mojavensis prefer pitaya agria cactus, Stenocereus gummosus, over all other potential hosts for feeding and breeding, including populations that inhabit areas where no agria grows. We sampled five geographically isolated populations of D. mojavensis from nature to assess host choice within and between populations. Host choice tests were performed in a laboratory “olfactometer” by allowing adult D. mojavensis to choose between plumes of synthetic volatile cocktails of two widespread host cacti. Overall, each population showed significant preference for agria volatiles with one exception: a mainland Sonora population that uses organ pipe cactus in nature exhibited preference for organ pipe volatiles, suggesting a possible shift in host preference. The degree of preference for agria volatiles was greatest in a population from southern California that use California barrel cactus as a host. Since southern Californian populations of D. mojavensis are thought to be derived from those in Baja California, preference for agria volatiles is considered a retained ancestral trait. Three populations from Baja California and mainland Mexico that use agria in the wild expressed lower, but similar preferences for agria volatiles. Because populations of D. mojavensis are ancestral to those in mainland Mexico, Arizona, and California, the shift from agria to alternate hosts has not been accompanied by strong changes in host preference behavior.
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  • 23
    ISSN: 1572-8889
    Schlagwort(e): Hymenoptera ; Leptopilina ; Drosophila ; semiochemicals ; kairomones ; parasitoid ; generalist ; specialist ; foraging behavior
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Foraging parasitoids are thought to need more specific information than generalists on the presence, identity, availability, and suitability of their insect host species. In the present paper, we compare responses to host kairomones by two phylogenetically related parasitoid species that attack Drosophilidae and that differ in the width of their host range. As predicted, the behavioral response of the parasitoids to host kairomones reflected their difference in host range. The response of the specialist parasitoid Leptopilina boulardiwas restricted to contact kairomones from its natural hosts and one closely related species. In contrast, the generalist parasitoid Leptopilina heterotomaresponded to contact kairomones of a variety of Drosophilidae species.
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  • 24
    ISSN: 1572-8889
    Schlagwort(e): courtship song ; wingbeat ; sexual isolation ; Drosophila
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 25
    ISSN: 1572-8889
    Schlagwort(e): courtship behavior ; songs ; sexual isolation ; Drosophila
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Four species of the Drosophila virilis group, D. montana, D. littoralis, D. lummei, and D. ezoana, occur sympatrically in several locations in northern Europe. Courtship interactions between the flies of the three first-mentioned species were observed at malt baits in Kemi, northern Finland, to find out how the flies of different species recognize conspecific individuals and how interspecific courtships differ from intraspecific ones in the wild. Intraspecific courtships (including females of different reproductive stages) and interspecific courtships were also videotaped and analyzed in laboratory. In the wild the males courted both conspecific and allospecific females, even though the species varied in how much the males were attracted to females of different species. Interspecific courtships usually broke off when the male touched the female or when the male and/or the female vibrated his/her wings, producing acoustic cues. In the laboratory males courted conspecific females irrespective of the reproductive stage of the female, even though the courtships directed toward immature and fertilized females usually included only orienting and touching (no licking and singing). D. littoralis, and very rarely D. montana and D. lummei, males courted also allospecific females. In the few interspecific courtships between these three species, where the male proceeded to singing, females responded to male singing by vibrating their wings. This ended the courtship. It is suggested that both the chemical cues affecting female attractivity and the acoustic signals of males and females, which are produced by wing vibration, function in maintaining sexual isolation between these three species.
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  • 26
    Digitale Medien
    Digitale Medien
    Springer
    Journal of insect behavior 13 (2000), S. 361-373 
    ISSN: 1572-8889
    Schlagwort(e): Drosophila ; copulatory courtship ; mate choice ; cryptic female choice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Two endemic Australian Drosophila species, D. birchii and D. serrata, have a copulatory courtship, i.e., the males court the female mainly during copulation. In the present study we found the males of both species to mount their prospective mating partners selectively, exhibiting both sex and species recognition. The males began to sing after mounting the female, and they often exhibited also postcopulatory displays typical to copulatory courtship. D. birchii and D. serrata females discriminated against males which did not sing during mounting/copulation, which suggests that the females utilize cryptic female choice. Our findings raise the question of how widespread a phenomenon cryptic female choice is in Drosophila species.
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  • 27
    ISSN: 1572-8889
    Schlagwort(e): host selection ; experience ; learning ; extinction ; reinforcement ; parasitoids ; Drosophila ; Leptopilina heterotoma ; Hymenoptera ; Eucoilidae
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The host-foraging behavior of female entomophagous parasitoids is commonly modified by positive associative learning. Typically, a rewarding experience (e.g., successful oviposition in a host) increases a female's foraging effort in a host microhabitat of the type associated with that experience. Less well understood are the effects of unrewarding experiences (i.e., unsuccessful foraging). The influence of unrewarding experience on microhabitat choice and residence time within a microhabitat was examined for the eucoilid parasitoid,Leptopilina heterotoma, in laboratory and greenhouse assays. As determined previously, females which oviposited successfully in either of two microhabitat types (fermenting apple or decaying mushroom) strongly preferred to forage subsequently on that microhabitat type. However, failure to find hosts in the formerly rewarding microhabitat caused females to reverse their preference in favor of a novel microhabitat type. The effect, though striking, was transient: within 1–2 h, the original learned preference was nearly fully restored. Similar effects of unrewarding experiences were observed with respect to the length of time spent foraging in a microhabitat. As determined previously, oviposition experience in a particular microhabitat type increased the time spent foraging in a patch of that microhabitat type. However, failure to find hosts in the patch caused the time a wasp spent in the next unoccupied patch of that type to decrease to almost nothing. In addition, there was a tendency for an unrewarding experience on a formerly rewarding microhabitat type to extend the time spent in a patch of a novel type. The function of the observed effects of unrewarding experiences is discussed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 28
    Digitale Medien
    Digitale Medien
    Springer
    Journal of insect behavior 1 (1988), S. 3-15 
    ISSN: 1572-8889
    Schlagwort(e): host preference ; habitat selection ; experience ; learning ; Drosophila ; host races ; population genetics
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A field experiment with Drosophila melanogasterrevealed that when flies encounter a particular food type soon after emergence, the probability of their subsequently being attracted to such a resource is increased. In this experiment, the length of time flies experienced their postemergence environments was under the control of the flies themselves. The experiment thus realistically mimicked one form of experiential effect that may be important in nature. A theoretical model is developed which shows that enhanced adult preferences for the types of resources fed on as larvae can substantially increase the degree of host-based genetic subdivision within a polyphagous population.
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  • 29
    ISSN: 1572-8889
    Schlagwort(e): Drosophila ; females ; sex appeal ; sexual receptivity ; fecundity
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The postcopulatory behavior of Drosophila biarmipes and Drosophila melanogaster females was analyzed and compared. Females from both species were shown to undergo a series of behavioral changes following mating, including significant reductions in both sexual attractiveness and receptivity. However, while both attractiveness and receptivity returned to “virgin-like” levels within a few days in D. melanogaster, D. biarmipes females, which regained their sexual attractiveness within a few days, remained unreceptive to copulation for at least 2 weeks. With respect to fecundity, D. melanogaster females produced more offspring when given opportunities to remate, while D. biarmipes females did not benefit from remating opportunities. These observations suggest that D. biarmipes females may have the ability to store sperm and produce offspring from a single mating over longer periods of time than other drosophilids.
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  • 30
    Digitale Medien
    Digitale Medien
    Springer
    Journal of insect behavior 8 (1994), S. 231-239 
    ISSN: 1572-8889
    Schlagwort(e): Drosophila ; sex ratio ; life history ; optimality model
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Based on both previously published literature and results reported here, it appears thatDrosophila melanogaster meet the explicit assumptions of the Trivers and Willard offspring sex allocation model. However, contrary to the model's predictions, offspring sex ratio was not significantly affected when we manipulated factors that influence offspring quality. We suggest that contrary to implicit predictions of offspring sex ratio models,Drosophila may lack the genetic plasticity to readily alter sex ratio.
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  • 31
    Digitale Medien
    Digitale Medien
    Springer
    Journal of insect behavior 9 (1996), S. 329-338 
    ISSN: 1572-8889
    Schlagwort(e): Drosophila ; size-related sexual selection ; yeast diet
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Size-related sexual selection (SRSS) was examined on four traits (thorax and wing length and head and face width) inDrosophila buzzatii, by scoring male copulatory status in two mass-mating experiments. Using axenic females, experiment 1 was carried out with axenic males, and experiment 2 with yeast-supplemented males. While there was no indication of SRSS in experiment 1, such selection was substantial in yeast-supplemented males, which transmitted yeasts to mating females. Multivariate analyses of selection indicated that face width is the measured trait on which directional SRSS essentially acted in yeast-supplemented males, resulting in indirect selection on body size. Because this selection was affected by yeast diet in males, its possible interaction with the yeast transmission from males to females during the courtship is discussed.
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  • 32
    Digitale Medien
    Digitale Medien
    Springer
    Journal of insect behavior 9 (1996), S. 505-516 
    ISSN: 1572-8889
    Schlagwort(e): courtship ; female behavior ; signals ; Drosophila
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Females of manyDrosophila species spread apart their wings prior to copulation. In the present study we found female wing spreading to provoke male copulation attempts inDrosophila virilis-group species, helping the males to attempt copulation when the female is ready to mate. The males of most species, however, rarely responded to female wing spreading by copulation attempt without licking the female genitalia before and/or after female wing spreading bout. Blocking the female genitalia (D. virilis, D. novamexicana) reduces males' tendency to attempt copulation after female wing spreading. In these, and most other species of the group, female wing spreading seems to be an efficient signal only when combined with stimuli from female genitalia.
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  • 33
    ISSN: 1572-8889
    Schlagwort(e): parasitoid ; superparasitism ; learning ; motivation ; egg load ; Drosophila
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The influence of egg-laying experience on the response of females of the eucoilid parasitoid,Leptopilina heterotoma, to parasitized and unparasitizedDrosophila melanogaster host larvae was examined under more controlled conditions than those used in past studies. In laboratory assays, we precisely manipulated both the number of eggs laid by females and the kind of larvae (parasitized versus unparasitized) in which the eggs were laid. We found that the tendency to avoid laying eggs in parasitized hosts depended markedly on whether or not eggs had been laid previously, but depended little on whether those eggs had been laid in parasitized or unparasitized hosts. The observed effect of general egg-laying experience on avoidance of parasitized hosts may reflect responses to either changes in the wasp's internal state (perhaps, changes in egg load) or changes in the wasp's neural representation of the external environment (such as those presumed to occur during learning). In light of these results, we offer a tentative reinterpretation of several earlier studies.
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  • 34
    Digitale Medien
    Digitale Medien
    Springer
    Journal of insect behavior 9 (1996), S. 477-491 
    ISSN: 1572-8889
    Schlagwort(e): Drosophila ; behavior ; polymorphism ; parasitoid wasp ; host defense
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The selection response of the polymorphic hostD. melanogaster (Meigen) to the braconid waspA. tabida (Nees) is addressed. Cages of flies with and without wasps were initiated with a population ofD. melanogaster that exhibited variation both in larval foraging behavior and in encapsulation ability. Encapsulation ability was measured as the proportion of parasitized larvae that produce a hardened capsule which encapsulates the wasp egg and ultimately kills the wasp larva. We determined whether the host population changed its encapsulation ability and/or its foraging behavior in response to the wasp. Both species were collected from a local orchard whereA. tabida is the only wasp known to parasitizeD. melanogaster larvae. The naturally occurring genetic polymorphism for rover and sitter larval foraging behavior inD. melanogaster is also found in this field population.A. tabida's vibrotactic search behavior enables it to detect rover more frequently than sitter larvae. Rover larvae move significantly more while feeding than do sitter larvae. In this field population, rover larvae also show higher encapsulation abilities than do sitter larvae. Six cage populations, three without wasps and three with wasps, each containing an equal mixture of rover and sitter flies, were established in the laboratory and maintained for 19 fly generations. Selection pressure in the laboratory was similar to that found in the field population from which the flies and wasps were derived. We found that larvae from cages with wasps developed a significantly higher frequency of encapsulation than those reared without wasps. We were, however, unable to detect a change in larval movement (rover or sitter behavior) in larvae from cages subject to selection from wasps compared to larvae from cages containing no wasps. This may have resulted from a balance between two selective forces, selection against rovers by the wasps' use of vibrotaxis, and selection for rovers resulting from their increased encapsulation abilities
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  • 35
    ISSN: 1572-8889
    Schlagwort(e): Drosophila ; sexual selection gradients ; courtship success
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Using wild-reared flies, we examined sexual selection on five phenotypic traits (thorax length, wing length, wing width, head width, and face width) inDrosophila buzzatii, by scoring copulatory status in nine mass mating cages. Only male face width was identified as a direct target of sexual selection in an analysis of selection gradient, while indirect selection was present on all other studied traits, as expected from their correlations with face width. In contrast to males, there was no indication of selection in females. Nor was there evidence of assortative mating. The suggested direct selection on face width seems to take place during licking behavior of the courtship and might be related to courtship feeding. This study suggests that courtship success gives rise to indirect selection on body size.
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  • 36
    Digitale Medien
    Digitale Medien
    Springer
    BioMetals 11 (1998), S. 359-372 
    ISSN: 1572-8773
    Schlagwort(e): calcium ; EGF-domains ; cadherins ; integrins ; calmodulin ; cytoskeleton ; Drosophila
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The known roles for calcium-binding proteins in developmental signaling pathways are reviewed. Current information on the calcium-binding characteristics of three classes of cell-surface developmental signaling proteins (EGF-domain proteins, cadherins and integrins) is presented together with an overview of the intra-cellular pathways downstream of these surface receptors. The developmental roles delineated to date for the universal intracellular calcium sensor, calmodulin, and its targets, and for calcium-binding regulators of the cytoskeleton are also reviewed.© Kluwer Academic Publishers
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  • 37
    ISSN: 1572-8773
    Schlagwort(e): metallothionein ; development ; metal induction ; Drosophila
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Expression of the two Drosophila melanogaster metallothionein genes, Mtn and Mto, has been analyzed by in situ hybridization during post-embryonic development. Mtn and Mto transcripts were detected exclusively in the digestive tract of larvae, pupae and adults reared on standard medium. Mtn and Mto expression domains overlap, but each gene is also expressed at unique sites. Mtn mRNA levels are approximately 10 and 20 times higher than those of Mto in larvae and adults, respectively. Copper and cadmium ions strongly induce Mtn and Mto mRNA accumulation in the midgut. Zinc is a weaker inducer, acting only at high concentrations. Mtn gene expression is induced by these three metals in Malpighian tubules, while Mto gene expression in this organ is induced only by zinc. Iron is a poor inducer of metallothionein mRNA accumulation. Functions of MTN and MTO proteins in metal homeostasis and detoxification are considered.
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  • 38
    ISSN: 1572-879X
    Schlagwort(e): aluminum oxide ; hydroxyl groups ; rhodium ; growth ; scanning tunneling microscopy ; infrared spectroscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Abstract In order to investigate how the presence of surface hydroxyl groups on oxide surfaces affects the interaction with the supported metal, we have modified a well-ordered alumina film on NiAl(110) by Al deposition and subsequent exposure to water. This procedure yields a hydroxylated alumina surface as revealed by infrared and high-resolution electron energy loss spectroscopy. By means of scanning tunneling microscopy, we have studied the growth of rhodium on the modified film at 300 K. Clear differences in the particle distribution and density are observed in comparison to the clean substrate. While, in the latter case, decoration of domain boundaries as typical defects of the oxide film governs the growth mode, a more isotropic island distribution and a drastically increased particle density is found on the hydroxylated surface. From infrared data, it can be deduced that the growth is connected with the consumption of the hydroxyl groups due to the interaction between the metal deposit and the hydroxylated areas. This finding is in line with photoemission results published earlier.
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  • 39
    ISSN: 1572-8889
    Schlagwort(e): Drosophila ; courtship song ; behavior ; female choice ; sexual isolation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The courtship behavior and the effects of courtship song in inter- and intraspecific crosses were studied in the four sympatric species of the Drosophila auraria complex: D. auraria, D. biauraria, D. subauraria, and D. triauraria. Orientation, tapping, and vibration (the repertoires of male courtship) were observed in both inter- and intraspecific crosses, suggesting that signals from heterospecific females were enough to elicit such male behaviors. The crossability tests with wingless or winged heterospecific males (tests for wing effects) revealed that winged heterospecific males copulated less than wingless ones in all four species but not all the pairwise cases. Since the crossability tests with aristaless females (deaf) or normal females showed essentially the same results as the tests for wing effects, we concluded that the sound produced by wing vibration plays an important role and that the wing movement itself is less important. These findings suggest that courtship songs are of great importance in mate discrimination and the sexual isolation between the species of this complex.
    Materialart: Digitale Medien
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  • 40
    ISSN: 1572-8889
    Schlagwort(e): Drosophila ; parasitoid wasp ; behavior ; genetics
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 41
    ISSN: 1572-8889
    Schlagwort(e): temperature preference ; Drosophila ; acclimation ; compensation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The effects of rearing and acclimation on the response of adultDrosophila to temperature were investigated in a gradient.D. melanogaster flies preferred a higher mean temperature and were distributed over a wider range of temperatures thanD. simulans flies. Acclimating adults at different temperatures for a week did not influence the response of either species. Adults reared at 28°C as immatures had a lower mean preference than those reared at cooler temperatures, suggesting that flies compensated for the effects of rearing conditions. Adults from tropical and temperate populations ofD. melanogaster andD. simulans did not differ in the mean temperature they preferred in a gradient, suggesting little genetic divergence for this trait within species. The species differences and environmental responses may be related to changes in optimal physiological conditions for the flies.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 42
    ISSN: 1572-8889
    Schlagwort(e): review ; Drosophila ; larva ; phototaxis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract In this paper we examine theDrosophila melanogaster larval response to light. We survey the morphology of the larval visual and motor systems in relation to larval locomotory behavior and phototaxis. In addition, this paper proposes a model of sensorimotor transformation and examines the reversal in taxis occurring at theD. melanogaster larval wnadering stage.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 43
    Digitale Medien
    Digitale Medien
    Springer
    Acta applicandae mathematicae 9 (1987), S. 219-237 
    ISSN: 1572-9036
    Schlagwort(e): 34A34 ; 34D99 ; 90A16 ; Nonlinear differential equations ; stability ; growth ; economic dynamics
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Mathematik
    Notizen: Abstract This paper analyses the implications of persistent growth upon the stability properties of dynamic models. Besides the traditional concept of asymptotic stability, new stability criteria-strong/weak absolute, strong/weak relative, strong/weak logarithmic stability-are introduced, and global stability conditions for satisfying these criteria are stated for general first-order autonomous differential equations. The conflict between rapidity of growth and the degree of stability is demonstrated. Economic applications of the stability theorems are illustrated within the growth models of Harrod and Solow.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 44
    Digitale Medien
    Digitale Medien
    Springer
    Cellular and molecular life sciences 41 (1985), S. 1554-1557 
    ISSN: 1420-9071
    Schlagwort(e): Immunocytochemistry ; neuropeptide Y ; radioimmunoassay ; rat pancreas
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Significant quantities of a newly discovered peptide, neuropeptide Y, were found in the rat pancreas, where they were localized to nerves in the exocrine parenchyma and around arterial and ductal structures. Although unaffected by surgical parasympathectomy, the periarterial and periductal nerves were abolished by chemical sympathectomy, suggesting that NPY is partially costored with sympathetic transmitters in nerve fibers.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 45
    Digitale Medien
    Digitale Medien
    Springer
    Cellular and molecular life sciences 41 (1985), S. 1621-1621 
    ISSN: 1420-9071
    Schlagwort(e): Chlorella ; cadmium ; growth
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Growth of 14 strains from fiveChlorella species is rather insensitive towards cadmium. One strain (211-1a) ofC. saccharophila, however, was found to have a sensitivity towards this toxic heavy metal about 100 times higher than that of the other strains ofC. saccharophila.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 46
    Digitale Medien
    Digitale Medien
    Springer
    Cellular and molecular life sciences 42 (1986), S. 1170-1173 
    ISSN: 1420-9071
    Schlagwort(e): Lampreys ; Geotria australis ; ammocoetes ; larval life ; growth ; metamorphosis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The average duration of larval life in the anadromous lamprey,Geotria australis (the sole representative of the Geotriidae) is estimated as 41/4 years. Compared with other lampreys, the ammocoetes ofG. australis have a slow growth rate, increase in length during the year preceding metamorphosis and typically enter metamorphosis at a small mean length (〈100 mm) and weight (〈1.2 g).
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 47
    Digitale Medien
    Digitale Medien
    Springer
    Cellular and molecular life sciences 47 (1991), S. 111-114 
    ISSN: 1420-9071
    Schlagwort(e): Drosophila ; repeat matings ; polyandrous pattern diversity ; sperm length
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary In order to test the validity of the prediction of the mating pattern of females from the sperm length distribution in males, three species ofDrosophila were analysed. Males in the three species are equally polygynous but females differ in the level of polyandry. A ‘low recurrence polyandry’ is observed in the sperm dimorphic speciesD. affinis while a ‘high recurrence polyandry’ is observed in the sperm monomorphic speciesD. latifasciaeformis andD. littoralis. These results are consistent with the hypothesis proposed previously that sperm dimorphism in males can only be maintained by a selective alternative in females (i.e. facultative female polygamy), whereas a stricter mating system (e.g., ‘obligatory’ polyandry) should only result in sperm monomorphism irrespective of the absolute value of sperm length.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 48
    Digitale Medien
    Digitale Medien
    Springer
    Cellular and molecular life sciences 49 (1993), S. 596-598 
    ISSN: 1420-9071
    Schlagwort(e): Drosophila ; hybridization ; male vigour ; male mating speed
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract Genetic variation has been found in males of aD. simulans population for their eagerness to hybridize withD. melanogaster females. In a search for traits involved in this hybridization, males ofD. simulans were tested for mating speed and sexual vigour. Between-male differences were detected in both sexual traits, but no relationship was noticed between them, nor with the frequency of hybridization. Thus male mating propensities appear to be unrelated to the breakdown of sexual isolation between these sibling species.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 49
    Digitale Medien
    Digitale Medien
    Springer
    Cellular and molecular life sciences 52 (1996), S. 503-510 
    ISSN: 1420-9071
    Schlagwort(e): Drosophila ; accessory gland ; reproduction ; sexual behavior ; sperm displacement ; evolution
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract Recent results from biochemical and molecular genetic studies of the accessory gland proteins in maleDrosophila are reviewed. The most prominent feature is the species-specific variability. However, the analysis of the sex peptide inD. melanogaster shows that there is a strong homology in the molecular structure to the closely related sibling species, and that divergence increases with increasing phylogenetic distance. For this reason the sex peptide, after being transferred to the female genital tract during copulation, reduces receptivity and increases oviposition only in virgin females belonging to the same species group and subgroup. Even though studies were hitherto limited to a small number of the secretory components, it is evident that the accessory gland proteins play a key role in reproductive success of the fruit fly by changing female sexual behavior, supporting sperm transfer, storage and displacement. Thus, genes encoding the accessory gland proteins are apparently under strong evolutionary selection.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 50
    Digitale Medien
    Digitale Medien
    Springer
    Cellular and molecular life sciences 52 (1996), S. 643-646 
    ISSN: 1420-9071
    Schlagwort(e): Aging ; development time ; stress ; energy cost ; oxidative stress ; Drosophila ; homeostasis ; life span
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract Life span and development time are considered in the context of the abiotic stresses to which free-living organisms are normally exposed. Under these circumstances, long life span depends upon metabolically efficient stress-resistance genes, which tend to be heterozygous. Similarly, rapid development time tends to be a feature of heterozygous stress-resistant individuals. Therefore, individuals who have high inherited stress resistance should develop fastest and live longest; in addition, they should show high homeostasis in the face of the energy costs of stress. In this way, the stress theory of aging can incorporate the developmental stage, based upon oxidative stress as an important major direct challenge.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 51
    Digitale Medien
    Digitale Medien
    Springer
    Cellular and molecular life sciences 52 (1996), S. 751-756 
    ISSN: 1420-9071
    Schlagwort(e): Chaperonin ; Drosophila ; groEL ; heat shock ; heat shock proteins ; HSP60 ; Malpighian tubules ; TCP-1
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract A homologue of the chaperonin protein of the HSP60 family has not been shown so far inDrosophila. Using an antibody specific to HSP60 family protein in Western blotting and immunocytochemistry, we showed that a 64-kDa polypeptide, homologous to the HSP60, is constitutively present in all tissues ofDrosophila melanogaster throughout the life cycle from the freshly laid egg to all embryonic, larval and adult stages. A 64-kDa polypeptide reacting with the same antibody in Western blots is present in all species ofDrosophila examined. Using Western blotting in conjunction with35S-methionine labeling of newly synthesized proteins and immuno-precipitation of the labeled proteins with HSP60-specific antibody, it was shown that synthesis of the 64-kDa homologue of HSP60 is appreciably increased by heat shock only in the Malpighian tubules, which are already known to lack the common HSPs.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 52
    ISSN: 1570-7458
    Schlagwort(e): Alsophila pometaria ; Geometridae ; Anisota senatoria ; Citheroniidae ; Quercus ; nutritional ecology ; herbivory ; nutritional indices ; consumption ; growth ; utilization efficiency ; nitrogen ; water ; tannins ; phenols ; gut pH ; digestive enzymes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Beschreibung / Inhaltsverzeichnis: Résumé Alimentées sur feuillage jeune de chêne, les chenilles d'Alsophila pometaria avaient un taux relatif de croissance (RGR) et un taux relatif d'accumulation d'azote (RNAR) plus élevés que les chenilles d'Anisota senatoria alimentées sur feuillage mûr de chêne. Bien que le jeune feuillage soit plus efficacement digéré par A. pometaria (AD plus élevé), il n'est pas assimilé et utilisé pour la croissance avec de meilleurs rendements (les ECI ne sont pas différents). Ainsi le taux de croissance plus élevé d'A. pometaria est dû entièrement à un taux de consommation plus important (RCR et RNCR). Le feuillage jeune est significativement plus riche en azote et en eau que le feuillage mûr, mais les niveaux de phénol et de tanins sont les mêmes. A pometaria consomme les feuilles de différentes espèces de chênes au même taux, indépendamment de la teneur en azote, tandis que A. senatoria accroît sa consommation en réponse à une diminution de la teneur en azote. Il en résulte que le taux de croissance d'A. pometaria dépend directement de la teneur en azote des feuilles, tandis que celui d'A. senatoria en est indépendant. Les systèmes digestifs des deux insectes sont biochimiquement semblables et sont efficaces pour la digestion des protéines. Les tanins et les phénols n'influent pas sur les indices nutritionnels de ces deux espèces. Nous estimons que le principal intérêt de l'alimentation printanière est la disponibilité en feuillage succulent, riche en azote, et non l'absence de feuilles à haute teneur en tanin. L'alimentation printanière semble correspondre à une strategie alimentaire qui favorise la croissance aux dépens de l'efficacité tandis que l'alimentation en fin d'été est une stratégie qui favorise l'efficacité sur la rapidité.
    Notizen: Abstract The larvae of Alsophila pometaria (Harr.), feeding on the young foliage of oak, has a higher relative growth rate (RGR) and relative nitrogen accumulation rate (RNAR) than the larvae of Anisota senatoria (J. E. Smith), feeding on the mature foliage of oak. Although the young oak foliage is more efficiently digested by A. pometaria (higher AD's), it is not more efficiently assimilated and used for growth (no difference in ECI's). Thus, the higher growth rate of A. pometaria is due entirely to a higher consumption rate (RCR and RNCR). Young foliage is significantly higher in nitrogen and water than mature foliage, but phenol and tannin levels are comparable in young and old foliage. A. pometaria consumes the foliage of different oak species at the same rate, independent of nitrogen content, while A. senatoria increases its consumption rate in response to decreased nitrogen levels. As a result, the growth rate of A. pometaria is directly related to leaf nitrogen content, while the growth rate of A. senatoria is independent of leaf nitrogen. The two species of insects have digestive systems that are very similar biochemically, and that are well-designed for effective protein digestion. Tannins and phenols do not influence the nutrional indices of either species. We suggest that the major benefit of spring feeding is the availability of succulent, high-nitrogen foliage, and not the avoidance of high-tannin foliage. The spring feeder appears to have a feeding strategy that favors rapid growth at the expense of efficiency, while the late summer feeder has a strategy that favors efficiency over rate.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 53
    Digitale Medien
    Digitale Medien
    Springer
    Entomologia experimentalis et applicata 39 (1985), S. 143-147 
    ISSN: 1570-7458
    Schlagwort(e): Hymenoptera ; Drosophila ; parasitoid ; habitat toxicity
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Beschreibung / Inhaltsverzeichnis: Résumé L'étude a porté sur la tolérance et l'utilisation de vapeurs d'éthanol, d'acide acétique et d'acétaldéhyde par Asobara persimilis (Hym. Braconidae), parasitoïde de Drosophila récemment découvert en Australie. Aux faibles concentrations, il n'y a pas d'utilisation significative d'éthanol et d'acide (respectivement moins de 1,5% et 0,1%), cependant la longévité des mâles et des femelles ont augmenté avec les concentrations d'acide acétique de 1,0 et 1,5%. Toutes ces substances sont toxiques à plus forte concentrations, bien qu'il y ait un dimorphisme sexuel et que les femelles survivent significativement plus longtemps que les mâles. La tolérance des braconides est inférieure à celle de leurs hôtes, les Drosophiles cosmopolites et endémiques à l'Australie. Ceci peut faire que ces parasites limitent l'exploitation de leurs hôtes aux habitats avec une faible concentration de produits de fermentation.
    Notizen: Abstract The tolerance and utilization of ethanol, acetic acid and acetaldehyde vapour was investigated in Asobara persimilis (Hymenoptera: Braconidae), a parasitoid of Drosophila. No significant utilization of ethanol or acetaldehyde occurred at low concentrations (〈 1.5% and 0.1% respectively), however both female and male longevity was increased at concentrations of 1.0 and 1.5% acetic acid. All substances were toxic at higher concentrations, but there was sexual dimorphism in that females survived significantly longer than males.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 54
    ISSN: 1570-7458
    Schlagwort(e): Homoptera ; Aphididae ; Acyrthosiphon pisum ; Medicago sativa ; resistance ; amino acids ; sucrose ; phloem sap ; artificial diet ; ingestion ; growth
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Beschreibung / Inhaltsverzeichnis: Résumé Sur des milieux holidiques se différenciant par leur concentration en acides aminés ou en saccharose ou par leur spectre en acides aminés et copiant la sève de deux variétés de luzerne (sensible et résistante) nous avons étudié différents paramètres biologiques d'un biotype d'Acyrthosiphon pisum Harris afin de mieux comprendre les mécanismes de résistance à ce puceron. Que ce soit pour le reproduction et la survie des adultes ou pour la croissance et le développment larvaire, nous montrons l'influence spécifique de la concentration en saccharose et en acides aminés ainsi que celle du spectre en acides aminés des régimes artificiels. Ces différences de performances de A. pisum peuvent en partie être expliquées par les différences d'ingestion. Néanmoins pour des rapport saccharose/acides aminés identiques et pour des niveaux d'ingestion égaux, il existe selon les milieux de grandes différences de taux de reproduction et donc d'efficacités métaboliques. Les milieux copiant la sève de variétés sensible et résistante permettent jusqu'à présent d'expliquer une partie des observations faites sur végétal, mais démontrent que l'ignorance de certains facteurs phagostimulants présents dans la plante empêche une interprétation complète des effets observés.
    Notizen: Abstract In order to understand the mechanisms of resistance of two lucerne cultivars (susceptible ‘Resistador’ and resistant ‘Lahontan’ clones) to a French biotype of Acyrthosiphon pisum Harris, several biological parameters of this aphid were investigated on sap-copied holidic diets differing either by their amino acid or sucrose concentrations or by the relative proportions of some amino acids. We recorded the quantitative influence of nutrient levels and amino acid profiles on adult survival and reproduction, as well as on larval growth and development. The differences in A. pisum performances on artificial diets may partly by explained by variable ingestion rates, but also by differences in metabolic efficiency of the ingested nutrients, which greatly depends on amino acid composition. Although holidic diet experiments partially confirm the biological assays of resistance on plants, they also point out a lack in our understanding of the factors present in the sap which are involved in phagostimulation in vivo, thus preventing a complete interpretation of observed resistance.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 55
    Digitale Medien
    Digitale Medien
    Springer
    BioMetals 5 (1992), S. 149-156 
    ISSN: 1572-8773
    Schlagwort(e): Hg2+ toxicity ; cyanobacterium ; Nostoc calcicola ; growth ; photopigments ; nucleic acids ; photosynthesis ; membrane integrity ; nutrient uptake ; enzymes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Toxicological responses of the filamentous N2-fixing cyanobacteriumNostoc calcicola Bréb. towards Hg2+ were studied to enumerate the decisive lethal events. In low-dose, long-term experiments (0.05–0.25 μm Hg2+, 10 days), photoautotrophic growth was severely inhibited with concurrent loss of photosynthetic pigments (phycocyanin〉chlorophyll α〉carotenoids) and nucleic acids. The termination of growth after a day 4 exposure to 0.25 μm Hg2+ has been attributed to the complete inhibition ofin vivo photosynthetic activity in the cyanobacterium (O2 evolution〉14CO2 incorporation). The elevated Hg2+ concentrations irreversibly damaged the cell membrance as observed under light microscopy, and as indicated by the leakage of intracellular electrolytes and phycocyanin. In high-dose, short-term experiments (0.5–20.0 μm Hg2+, up to 6 h), thein vivo activities of selected enzymes (glutamine synthetase 〉 nitrate reductase 〉 nitrogenase) were less inhibited by Hg2+ than the uptake of nutrient ions (NH 4 + 〉NO 3 − 〉PO 4 3− ).
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 56
    Digitale Medien
    Digitale Medien
    Springer
    Entomologia experimentalis et applicata 35 (1984), S. 75-81 
    ISSN: 1570-7458
    Schlagwort(e): Nezara viridula ; insect dietetics ; growth ; fecundity ; flight capacity ; Heteroptera
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Beschreibung / Inhaltsverzeichnis: Résumé Les effets du régime alimentaire pendant le 5ème stade sur les performances imaginales de N. viridula ont été déterminés en comparant la croissance, la fécondité, la durée du vol captif, et l'accumulation de corps gras chez des insectes élevés sur 4 régimes stérilisés. A partir de la formation des larves de 5ème stade, elles ont été alimentées sur haricots verts et arachides, ou transférées sur un régime formé exclusivement d'arachides, ou de haricots verts, ou de graines de soja ‘trempées’, cependant la durée du 5ème stade. Les insectes ont été transférés sur un régime de haricots verts et arachides à leur émergence. Les femelles élevées continuellement de haricots verts et arachides étaient plus lourdes (P〈0,01) à l'émergence (161,6 mg) que celles élevées sur haricots verts (146,3 mg) pendant le 5ème stade, et pondent plus d'oeufs (P〈0,08) pendant une durée de 34 jours (100,9 oeufs) que celles élevées sur arachides (54,7 oeufs). Plus de femelles élevées sur haricots verts et arachides (76,2%) pondent que celles élevées sur arachides (61,1%), soja ‘trempé’ (62,5%), ou haricots verts (60,9%). Les femelles élevées sur arachides volaient plus longtemps (P 〈 0,01) (42,9 mn) que celles élevées sur haricots verts (19,8 mn) pendant des vols captifs le 3ème jour après l'émergence. 78% de toutes les femelles élevées sur arachides volaient 〉-30 mn, contre 50% pour celles élevées sur haricots verts et arachides, ou soja ‘trempé’, et 35% pour celles élevées sur haricots verts. Seulement 7% des femelles élevées sur haricots verts volaient à la 60ème minute à la fin du test, contre 25% pour les femelles soja ‘trempé’, 33% pour les femelles haricots verts et arachides, et 47% pour celles élevées sur arachides. Les femelles ont été disséquées 34 jours après l'émergence pour déterminer l'importance du corps gras. Les indices de corps gras (Kiritani, 1963) montraient que les femelles élevées sur arachides (1,8) et graines de soja (1,7) ont apparemment un corps gras plus développé que celles élevées sur haricots verts et arachides (1,5), our haricots verts (1,2), pendant le 5ème stade. Ces résultats montrent que l'alimentation pendant le dernier stade larvaire de N. viridula affecte irréversiblement la croissance, la fécondité et l'aptitude au vol des femelles. En estimant que des résultats semblables auraient été obtenus avec des insectes élevés sur ces 4 aliments depuis l'éclosion, haricots verts et arachides constituent le meilleur aliment pour l'élevage de N. viridula. Ce régime fournit une graine mûre, vraisemblablement nécessaire pour l'accumulation d'un corps gras suffisant et une aptitude au vol migratoire, et une plante verte qui fournit les aliments apparement essentiels à l'ovogenèse.
    Notizen: Abstract The effects of diet during the fifth stadium on performance of adult N. viridula were determined through comparing growth, fecundity, and duration of tethered flight of insects reared on four axenic diets: fresh green bean, raw-shelled peanut, a combination of the two and soaked soybean seed. Females reared on the combination diet were heavier than those reared on green beans and oviposited more than those reared on peanuts. Three-day-old adult females reared on peanuts flew longer than those reared on green beans. More females reared on diets containing mature seed showed a capacity for extended flight and had larger fat bodies at 34-days postemergence. Thus, inadequate diet during the fifth stadium irreversibly affected growth, development, reproduction and flight capacity.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 57
    Digitale Medien
    Digitale Medien
    Springer
    Entomologia experimentalis et applicata 41 (1986), S. 121-133 
    ISSN: 1570-7458
    Schlagwort(e): Ips typographus ; bark beetle ; Scolytidae ; intra-specific larval competition ; growth ; simulation model ; breeding pattern
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Die Konkurrenz zwischen Larven des Borkenkäfers Ips typographus L. in Rundhölzern von Picea abies Karsten wurde im Laboratorium studiert. Um die Annahme zu testen, dass die Mortalität bei Borkenkäferlarven hauptsächlich von Futterkonkurrenz stammt, wurden die Parameter dieses Vorgangs geschätzt, die Zahl der Überlebenden vorausgesagt und verglichen mit der tatsächlichen Zahl Überlebender in jedem Rundholz. Die erforderlichen Voraussagen wurden mit dem Simulationsmodell von De Jong & Saarenmaa (1985) gemacht. In diesem Modell wird angenommen, dass die Larven Futter aufnehmen, in dem sie sich in der zweidimensionalen Rindenschicht bewegen und dass sie sterben oder sich vorzeitig in untergewichtige Käfer verwandeln, wenn sie in ein Gebiet gelangen, dessen Futter erschöpft ist. Es wurde geschlossen, dass der wichtigste Aspekt der Larvenbewegung im Hinblick aufs Überleben das gegenseitige Ausweichen ist. Die Muster der Larvengänge, wie sie aus einem Muttergang aufgrund gegenseitigen Ausweichens entstehen, wurden berechnet; sie stimmten sehr gut überein mit den beobachteten Mustern. Das Wachstum und das Überleben wurden in separaten Versuchen untersucht. Es wurde gezeigt, dass vorzeitiger Entwicklungsabschluss als Folge von Nahrungsknappheit eintritt. Das Überleben hängt davon ab, ob die Larve genug Futter zum Verpuppen erhalten hat. Dass die Larve eine bestimmte Grösse erreicht (Wachstum), war proportional zur Bewegung über eine bestimmte Distanz in der Rindenschicht. Unter den Versuchsbedingungen nahm das potentielle Trockengewicht der Puppen um 0,08 mg zu mit jedem mm Larvengang. Die minimale zur Verpuppung erforderliche Futtermenge war 20 mm Ganglänge, was proportional zu 1,6 mg Puppentrockengewicht ist. Aus diesen Resultaten wird geschlossen, dass Futterkonkurrenz die hauptsächliche Mortalitätsursache unter Larven von I. typographus in Rundhölzern im Laboratorium ist. Zudem wurde das Simulationsmodell erfolgreich überprüft; deshalb scheint es, dass Futterkonkurrenz entsprechend der vorigen Hypothese erfolgt. Die Annahme scheint vertretbar, dass dieser letzte Schluss auch für I. typographus unter natürlicheren Bedingungen zutrifft.
    Notizen: Abstract A series of laboratory experiments were conducted to provide parameters for a simulation model for Ips typographus L. (Coleoptera, Scolytidae). This model illustrates larval competition and predicts brood survival in bolts. Brood survival depended upon larvae attaining a weight of at least 1.6 mg dry weight. The pattern of larval foraging in a bolt is hypothesized to be regulated by mutual avoidance among larvae. An analytical solution is presented for the construction of the pattern of larval paths originating from a single maternal gallery.
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  • 58
    ISSN: 1570-7458
    Schlagwort(e): Syrphidae ; Metasyrphus corollae ; cereal aphid ; Sitobion avenae ; quantification ; field populations ; biomass ; voracity ; growth ; development ; kill rate
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Beschreibung / Inhaltsverzeichnis: Résumé Le taux de mortalité de S. avenae provoqué par les larves de M. corollae a été mesuré au laboratoire pour évaluer l'impact de ce prédateur sur les populations de ce puceron dans la nature. L'évolution du poids des larves et de la biomasse de pucerons tués a été suivie tous les jours dans des conditions de température et humidité constantes. Un taux moyen de mortalité a été déterminé pour chaque stade larvaire du prédateur, il a servi à déduire les niveaux de mortalité quotidienne dans les champs.
    Notizen: Abstract The rate of kill of cereal aphids by larvae of Metasyrphus corollae (F.) (Diptera: Syrphidae) was measured in the laboratory with the aim of assessing the impact of this predator on field populations of cereal aphids. Daily measurements were taken of changes in both the larval weight and the biomass of aphids killed by M. corollae in conditions of constant temperature and humidity. A mean relative kill rate was defined and calculated for each larval instar of the predator from which estimates of field daily rates of kill can be made.
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  • 59
    Digitale Medien
    Digitale Medien
    Springer
    Entomologia experimentalis et applicata 43 (1987), S. 193-201 
    ISSN: 1570-7458
    Schlagwort(e): Leptopilina boulardi ; Cynipidae ; Hymenoptera ; parasitoid ; Drosophila ; Diptera ; field egg laying strategy ; functional response ; switching
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Beschreibung / Inhaltsverzeichnis: Résumé Le concept de réponse optimale d'un parasite vis-à-vis de l'hôte le plus favorable pour son développement demeure surtout théorique et n'a pu être vérifié que dans les conditions de laboratoire. Nous avons montré que Drosophila melanogaster s'avère être, par rapport à D. simulans, l'hôte le plus favorable pour le développement du cynipide parasite Leptopilina boulardi. Une étude sur le terrain a démontré que ce parasite présente une réponse fonctionnelle densité dépendante vis-à-vis de D. melanogaster et non vis-à-vis de D. simulans, avec un effet de bascule. D'autre part, il s'avère que ce parasite exploite beaucoup mieux son hôte, en évitant le superparasitisme, ceci étant démontré au laboratoire et dans la nature. Enfin, il apparaît qu'il est capable d'allonger sa période de ponte lorsque cet hôte est rare, ce qui ne se produit pas avec D. simulans.
    Notizen: Abstract The hypothesis of optimal host species selection predicts that when a parasitoid has the choice between two host species, it will choose the species thay gives the best survival chances for its progeny. We confirmed this hypothesis by laboratory experiments with Leptopilina boulardi Barb. et al., a cynipid parasitoid which prefers Drosophila melanogaster Meigen (the host species most suitable for parasitoid survival) above D. simulans Sturt. As far as fitness parameters are concerned, the fertility of L. boulardi is higher with D. melanogaster; the egg laying can be spread out over a long period when this host is relatively scarce. This does not occur with D. simulans in which parasitic oviposition stops soon when this host is not abundant. Investigations of this foraging strategy were done under more complex natural conditions. We found that L. boulardi has a type III functional response with D. melanogaster only; furthermore, it seems that a switching effect may exist with this host. Parasitoid females appear to distribute their eggs more regularly on D. melanogaster, thus avoiding superparasitism. This seems to be independent of the relative frequency of this host. However, superparasitism of D. simulans did occur more frequently when this host was scarce.
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  • 60
    Digitale Medien
    Digitale Medien
    Springer
    Entomologia experimentalis et applicata 49 (1988), S. 235-244 
    ISSN: 1570-7458
    Schlagwort(e): Coccinellidae ; Harmonia axyridis ; Semiadalia undecimnotata ; Aphids ; Acyrthosiphon pisum ; Pyralidae ; Anagasta kuehniella ; growth ; reproduction ; breeding ; natural prey ; unnatural prey
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Beschreibung / Inhaltsverzeichnis: Summary Aphidophagous coccinellids Harmonia axyridis and Semiadalia undecimnotata were reared on eggs of the pyralid moth, Anagasta kuehniella during three generations previously killed by exposure to ultra violet radiation. Incidentally, these eggs are used for the mass production of Trichogramma. For H. axyridis larvae, this prey has the same nutritional value as the aphid Acyrthosiphon pisum (control). In adults, it causes an increase in reproductive activity which persisted during three generations. Decreased egg fertility is compensated for by stimulated oviposition. However, regardless of generations, the number of larvae produced per female per day remained equal to or became larger than the control. When the adults are transferred to aphid prey after 15 days of oviposition on the substitution food, a high level of fecundity is maintained and egg fertility improved, particulary during the last two generations. In S. undecimnotata, a specific aphid predator, preying on substitute prey is associated with prolonged pre-imaginal development and increased mortality, the latter affecting up to 50% of the larvae during the third generation. In adults, it induces a deterioration of the reproductive capacities (fecundity, fertility) which however seemed to improve during the third generation. Substitute prey has negative effect on these adults. At present, only H. axyridis might be considered for temporary mass production, as suggested by the results obtained at the end of the first generation.
    Notizen: Résumé Les coccinelles aphidiphages Harmonia axyridis Pallas et Semiadalia undecimnotata Schneider (Coccinellidae) ont été élevées pendant trois générations à l'aide d'oeufs d'Anagasta kuehniella Zell. (Pyralidae) tués par une exposition à un rayonnement ultraviolet. Chez la première espèce, cette proie de substitution présente une valuer alimentaire comparable si ce n'est pas supérieure à celle du puceron Acyrthosiphon pisum (Harris). Ces résultats permettent d'envisager, dès à présent, une production massive dans ces conditions trophiques. La mise en place d'un élevage continu dépend de l'évolution sur un grand nombre de générations de certains paramètres biologiques notamment de la fertilité des femelles. Chez la seconde espèce, une telle application paraît impossible pour l'instant.
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  • 61
    Digitale Medien
    Digitale Medien
    Springer
    Entomologia experimentalis et applicata 47 (1988), S. 81-88 
    ISSN: 1570-7458
    Schlagwort(e): imaginal diapause ; male mating activity ; genetics ; Drosophila
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Beschreibung / Inhaltsverzeichnis: Résumé Les femelles de D. triauraria Bock & Wheeler (Dipt. Drosophilidae) sont connues pour présenter une diapause reproductrice aux photophases courtes. Les mâles eux aussi ont révélé une activité sexuelle réduite aux photophases courtes, c'est-à-dire qu'ils sont entrés comme les femelles en diapause reproductive. Les photophases critiques pour l'induction de la diapause des mâles et des femelles n'ont pas présenté de différences. Les diapause des mâles et des femelles s'achèvent même sous courtes photophases, mais la diapause mâle était quelque peu plus faible que la diapause femelle. La photophase critique et le taux de diapause ont varié en fonction de l'origine géographique dans l'espèce actuelle. Lors de croisements entre lignées diapausantes et non-diapausantes, la photophase critique et la durée de la diapause ont été héritées quantitativement. A partir de ces expériences et d'expériences précédentes de croisements (Kimura, 1983), quelques modèles de méchnisme d'induction de la diapause de cette espèce sont proposés.
    Notizen: Abstract In Drosophila triauraria Bock & Wheeler (Diptera: Drosophilidae) of which females were known to enter reproductive diapause at short daylengths, males also showed reduced mating activity at short daylengths, i.e., males as well as females entered reproductive diapause. The critical daylength for diapause induction did not differ between females and males. Both male and female diapause ended even under short daylengths, but the male diapause was somewhat weaker than the female diapause. The critical daylength and the diapause rate varied geographically in this species. In the cross between diapausing and non-diapausing strains, the critical daylength and the diapause duration inherited in a quantitative manner. On the basis of the present and previous crossing experiments, some models are proposed on the mechanism of diapause induction of this species.
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  • 62
    Digitale Medien
    Digitale Medien
    Springer
    Entomologia experimentalis et applicata 29 (1981), S. 109-116 
    ISSN: 1570-7458
    Schlagwort(e): Heteronychus arator ; Scarabaeidae ; larvae ; consumption ; utilization ; growth ; feeding ; roots ; Paspalum dilatatum ; Lolium perenne ; Trifolium repens
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Die Futteraufnahme, die Futterverwertung und das Wachstum von Drittstadiumlarven von H. arator beim Fressen von Wurzeln von Paspalum dilatatum, Raygras (Lolium perenne) und Weissklee (Trifolium repens) wurden gravimetrisch gemessen. Der durchschnittliche Futterverzehrindex auf Gräsern war 0.65 mg/mg Larve/Woche verglichen mit 0.18 auf Weissklee. Die relative Wachstumsrate auf Paspalum und Raygras was 0.158 und 0.124 mg/mg Larve/Woche verglichen mit 0.043 auf Weissklee, was den geringen Verzehr an Weisskleewurzel widerspiegelt. Die Verwertung der gefressenen Wurzel war 22%, 18% und 24% für Paspalum, Ryagras und Weissklee. Das Fehlen gesicherter Unterschiede in der Futterverwertung der drei Pflanzenarten deutet darauf hin, dass die geringe Wachstumsrate auf Weissklee bloss durch geringe Futteraufnahme verursacht ist. Es wurde gezeigt, dass in Weisskleewurzel Frasinhibitoren für H. arator vorhanden sind. Die erhaltenen Schätzungen für Futteraufnahme durch Scarabaeiden sind die besten Daten für wurzelfressende Arten und liefern eine Basis, um den Einfluss der Weidepflanzen auf H. arator Populationen zu verstehen.
    Notizen: Abstract The consumotion, utilization and growth of third-instar larvae of black beetle, Heteronychus arator (F.) feeding on roots of ryegrass (Lolium perenne), paspalum (Paspalum dilatatum) and white clover (Trifolium repens) were studied. Consumption was greater on the grasses than on white clover when assessed by gravimetric methods. Larvae had a higher growth rate on the grasses. Utilization of the roots of the three species was similar indicating that poor growth on white clover is the result of low consumption probably induced by the presence of feeding deterrents in the roots.
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  • 63
    Digitale Medien
    Digitale Medien
    Springer
    Entomologia experimentalis et applicata 48 (1988), S. 225-232 
    ISSN: 1570-7458
    Schlagwort(e): Avena macrostachya ; Hordeum bogdani ; resistance ; Rhopalosiphum padi ; growth ; excretion ; amino acids ; leaf anatomy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Beschreibung / Inhaltsverzeichnis: Résumè Lors d'examens systématiques antérieurs, Avena macrostachya (Bal. ex Coss. & Dur.) et Hordeum bogdani (Wil.) ont présenté une résistance élevée au puceron Rhopalosiphum padi (L.). Lorsqu'ils avaient un choix comprenant de l'avoine et de l'orge cultivés, les pucerons ailés ont atterri en nombres moins importants sur les espèces sauvages. Les résultats étaient cependant variables dans le complexe avoine. La production de nymphes et le poids des adultes étaient plus élevés sur espèces cultivées, ainsi que la durée du développement était plus longue sur les espèces sauvages. A partir du troisième stade, l'excrétion de miellat a été significativement plus faible sur les espèces résistantes. En général, le miellat y contenait moins de 1% d'acides aminés bien que sur H. vulgare il en contînt 3,5%. Les pourcentages d'acides aminés libres du miellat étaient semblables sur toutes les plantes (5,2–7,6%), à l'exception de H. vulgare sur lequel les pucerons excrétaient 22% des taux ingérés. Les acides aminés excrétés en fortes quantités sur les différentes plantes, comprenaient l'asparagine, l'acide γ-aminobutyrique, l'acide glutamique et la glycine. Des coupes de tissus n'ont révélé aucun obstacle mécanique clair à la pénétration des stylets. Les possibilités d'utiliser ces espèces sauvages comme source de résistance aux pucerons dans la sélection de l'avoine et de l'orge ont été examinées.
    Notizen: Abstract In previous screening tests the two wild crop relatives Avena macrostachya (Bal., ex Coss. et Dur.) and Hordeum bogdani (Wil.) demonstrated a high degree of resistance to the aphid Rhopalosiphum padi (L.). In a choice situation using wild and cultivated oats and barley, alate aphids settled in lower numbers on the wild species. The results were, however, variable in the Avena combination. Nymph production was significantly higher, development time shorter and adult weight higher on the cultivated varieties. From the third instar and onwards the excretion of honeydew was significantly lower on the resistant plants. In general the honeydew contained less than 1% free amino acids although excreta from H. vulgare contained 3.5%. The percentage of free amino acids found in the honeydew was similar for all plant species (5.2–7.6%) except for H. vulgare, on which the aphids excreted 22% of the amounts ingested. Amino acids excreted in high proportions on all plants included asparagine, γ-aminobutyric acid, glutamic acid, and glycine. Tissue sectioning did not reveal any obvious mechanical barriers to stylet penetration. The potential use of these wild species as sources for aphid resistance breeding in oats and barley is considered.
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  • 64
    Digitale Medien
    Digitale Medien
    Springer
    Entomologia experimentalis et applicata 61 (1991), S. 59-72 
    ISSN: 1570-7458
    Schlagwort(e): Consumption ; food utilization efficiencies ; growth ; Lycopersicon esculentum ; Manduca sexta ; Nicotiana tabacum ; potassium ; tobacco hornworm ; water regulation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Third instar tobacco hornworms (Manduca sexta L.: Sphingidae) on low dietary potassium had a lower relative growth rate than individuals on diets with potassium concentrations reflecting those in host-plants, due to decreased consumption rate, lower efficiencies of conversion of ingested and digested food (ECI and ECD), and a prolonged growth/feeding phase. Furthermore, these larvae, when placed on a diet with a moderate potassium concentration through the fourth stadium, ended up being smaller due to lower ECI and less biomass gained, and had a prolonged growth phase, which suggest an irreversible cost of the previous low potassium diet. Third instar hornworms on high potassium diets had lower ECI and ECD, and they had a prolonged growth phase. These individuals, when placed on a moderate potassium diet in the fourth stadium, gained less biomass, than those previously offered hostplant-like-potassium diets. Body potassium concentrations (% dw) at the end of the third stadium were similar among treatment groups. With increasing potassium concentrations in the diet, utilization efficiencies of potassium decreased and potassium concentrations in the frass increased. Correspondingly, water content (% fw) of the newly-molted fourth instar larvae declined with increasing potassium, indicating a passive loss of water during potassium excretion. Low and high dietary potassium reduced survivorship of third instar larvae; fourth instar caterpillars previously fed the low potassium diet also had poor survivorship. We conclude that, within the normal range of potassium concentrations in the hostplants, caterpillar performance is largely unaffected by potassium concentration, but that potassium-poor and potassium-rich diets, such as those hornworms may sometimes experience, can reduce growth and survivorship.
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  • 65
    Digitale Medien
    Digitale Medien
    Springer
    Calcified tissue international 12 (1973), S. 313-321 
    ISSN: 1432-0827
    Schlagwort(e): Cathepsin D ; Extracellular ; Ossification ; Immunocytochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin , Physik
    Beschreibung / Inhaltsverzeichnis: Résumé De la cathepsine D extracellulaire d'origine principalement ostéoblastique a été démontrée par immunohistochimie dans le cartilage en voide de calcification de cultures d'os des membres d'embryons de poulet. L'intérêt de ce fait pour l'ossification est discuté.
    Kurzfassung: Zusammenfassung Es wurde mit einer immunohistochemischen Methode gezeigt, daß extrazelluläres Kathepsin D, welches hauptsächlich aus Osteoblasten gewonnen wurde, Knorpel von kultivierten Knochen von Kükenembryonen mineralisiert. Die Bedeutung dieser Feststellung für den Mechanismus der Knochenbildung wird diskutiert.
    Notizen: Abstract Extracellular cathepsin D derived mainly from osteoblasts has been demonstrated immunohistochemically in ossifying cartilage of cultured embryonic chick limb bones. The relevance of this observation to the mechanism of ossification is discussed.
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  • 66
    Digitale Medien
    Digitale Medien
    Springer
    Journal of molecular evolution 41 (1995), S. 1152-1159 
    ISSN: 1432-1432
    Schlagwort(e): Synonymous substitutions ; Nonsynonymous substitutions ; Estimation methods ; Confidence intervals ; Drosophila
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A method for estimating the numbers of synonymous (Ks) and nonsynonymous (Ka) substitutions per site is proposed. The method is based on the Li's (J Mol. Evol. 36:96–99, 1993) and Pamilo and Bianchi's (Mol. Biol. Evol. 10:271–281, 1993) method, but a putative source of bias is solved. It is proposed that the number of synonymous substitutions that are actually transitions or transversions should be computed by separating the twofold degenerate sites into two types of sites, 2S-fold and 2V-fold, where only transitional and transversional substitutions are synonymous, respectively. Kimura's (J. Mol. Evol. 16:111–120, 1980) two-parameter correcting method for multiple substitutions at a site is then applied using the overall observed synonymous transversion frequency to estimate both the numbers of synonymous transversional (Bs) and transitional (As) substitutions per site. This approach, therefore, also minimizes stochastic errors. Computer simulations indicate that the method presented gives more accurate Ks and Ka estimates than the aforementioned methods. Furthermore, the obtention of confidence intervals for divergence estimates by computer simulation is proposed.
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  • 67
    ISSN: 1432-1432
    Schlagwort(e): Evolution ; Expression patterns ; α-Amylase ; Glucose repression ; Starch induction ; Intra- and interspecific variation ; Drosophila ; Gene expression ; Regulatory genes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Intraspecific variation of α-amylase activity in D. melanogaster and D. immigrans, which is distantly related to D. melanogaster, and interspecific variation of α-amylase activity in 18 Drosophila species were examined. The amount of intraspecific variation of α-amylase activities measured in terms of coefficient of variation in D. melanogaster and D. immigrans was one-half and one-tenth or less, respectively, of the interspecific variation in 18 Drosophila species. We also surveyed the response patterns of α-amylase activity to dietary carbohydrates at the larval and adult stages. The levels of α-amylase activity depended on both repression by dietary glucose (glucose repression) and induction by dietary starch (starch induction). In general, our data suggest that glucose repression was conserved among species at both stages while starch induction was mainly observed in larvae, although the degree of the response depended on species. In D. lebanonensis lebanonensis and D. serrata, larvae expressed electrophoretically different α-amylase variants (isozymes) from those of adult flies. These results may suggest that the regulatory systems responsible both for the response to environment and developmental expression are different among species in Drosophila.
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  • 68
    Digitale Medien
    Digitale Medien
    Springer
    Journal of molecular evolution 28 (1988), S. 145-150 
    ISSN: 1432-1432
    Schlagwort(e): Alcohol dehydrogenase ; Drosophila ; Enzyme kinetics ; Product inhibition ; Microevolution
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Because natural populations ofDrosophila melanogaster are polymorphic for different allozymes of alcohol dehydrogenase (ADH) and becauseD. melanogaster is more tolerant to the toxic effects of ethanol than its sibling speciesD. simulans, information regarding the sensitivities of the different forms of ADH to the products of ethanol degradation are of ecological importance. ADH-F, ADH-S, ADH-71k ofD. melanogaster and the ADH ofD. simulans were inhibited by NADH, but the inhibition was relieved by NAD+. The order of sensitivity of NADH was ADH-F〈ADH-71k, ADH-S〈ADH-simulans with ADH-F being about four times less sensitive than theD. melanogaster enzymes and 12 times less sensitive than theD. simulans enzyme. Acetaldehyde inhibited the ethanolto-acetaldehyde activity of the ADHs, but at low acetaldehyde concentrations ethanol and NAD+ reduced the inhibition. ADH-71k and ADH-F were more subject to the inhibitory action of acetaldehyde than ADH-S and ADH-simulans, with ADH-71k being seven times more sensitive than ADH-S. The pattern of product inhibition of ADH-71k suggests a rapid equilibrium random mechanism for ethanol oxidation. Thus, although the ADH variants only differ by a few amino acids, these differences exert a far larger impact on their intrinsic properties than previously thought. How differences in product inhibition may be of significance in the evolution of the ADHs is discussed.
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  • 69
    Digitale Medien
    Digitale Medien
    Springer
    Journal of molecular evolution 28 (1989), S. 220-224 
    ISSN: 1432-1432
    Schlagwort(e): Drosophila ; Foldback element ; Transposable element
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Foldback elements are a family of transposable elements described inDrosophila melanogaster. The members of this dispersed repetitive family have terminal inverted repeats that sometimes flank a central region. The inverted repeats of all the family members are homologous. The study of the distribution and conservation of the foldback elements in differentDrosophila species shows that this distribution is different from that of the hybrid dysgenesis systems (PM and IR). Sequences homologous to foldback elements were observed by Southern blots and in situ hybridization in all species of themelanogaster subgroup and in some species of themontium andtakahashii subgroups. The element was probably already present before the radiation of these subgroups. No evidence of horizontal transmission of the foldback element could be observed.
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  • 70
    Digitale Medien
    Digitale Medien
    Springer
    Journal of molecular evolution 33 (1991), S. 156-162 
    ISSN: 1432-1432
    Schlagwort(e): Drosophila ; Mitochondrial DNA ; Nucleotide sequence ; Nonsynonymous substitutions ; Phylogeny ; A+T content
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The nucleotide sequence of a segment of the mitochondrial DNA from threeDrosophila species (D. erecta, D. eugracilis, andD. takahashii), belonging to different subgroups of themelanogaster group has been determined. The segment encompasses three complete tRNA genes (tRNAtrp, tRNAcys, and tRNAtyr) and portions of two protein-coding genes: the subunit 2 of the NADH dehydrogenase (ND2) and the subunit 1 of the cytochrome oxidase (COI). Comparisons also involve homologous sequences already known for four otherDrosophila species of themelanogaster group. Length differences were confined in the intergenic region where a long stretch of AT repeats was observed in one of the species analyzed. The three tRNA genes exhibit very different evolutionary rates, the most slowly evolving one, tRNAtyr, is adjacent to the 5′ end of COI; tRNAs in similar positions have been previously shown to evolve slowly because they are probably involved in transcript processing. Although the rate of synonymous substitutions was very similar between ND2 and COI genes there were strong discrepancies between them in terms of the number of nonsynonymous substitutions. Differences have also been found in G+C content of the genes, which are likely to be linked to different selective pressures. There is a reduction in G+C content in the region where selective constraints are reduced. This suggests the existence of different levels of constraints along the sequenced segment. An overall analysis of the types of substitutions showed a decrease in A+T content during the course of evolution of the species.
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  • 71
    Digitale Medien
    Digitale Medien
    Springer
    Journal of molecular evolution 22 (1985), S. 252-271 
    ISSN: 1432-1432
    Schlagwort(e): Drosophila ; Mitochondrial DNA ; Mitochondrial genes ; Nucleotide sequence ; Gene arrangement ; Genetic code ; Codon-anticodon interaction ; Ribosomal RNA genes ; Transfer RNA genes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The sequence of the 16,019 nucleotide-pair mitochondrial DNA (mtDNA) molecule ofDrosophila yakuba is presented. This molecule contains the genes for two rRNAs, 22 tRNAs, six identified proteins [cytochrome b, cytochrome c oxidase subunits I, II, and III (COI-III), and ATPase subunits 6 and 8] and seven presumptive proteins (URF1-6 and URF4L). Replication originates within a region of 1077 nucleotides that is 92.8% A+T and lacks any open reading frame larger than 123 nucleotides. An equivalent to the sequence found in all mammalian mtDNAs that is associated with initiation of second-strand DNA synthesis is not present inD. yakuba mtDNA. Introns are absent fromD. yakuba mitochondrial genes and there are few (0–31) intergenic nucleotides. The genes found inD. yakuba and mammalian mtDNAs are the same, but there are differences in their arrangement and in the relative proportions of the complementary strands of the molecule that serve as templates for transcription. Although theD. yakuba small and large mitochondrial rRNA genes are exceptionally low in G and C and are shorter than any other metazoan rRNA genes reported, they can be folded into secondary structures remarkably similar to the secondary structures proposed for mammalian mitochondrial rRNAs.D. yakuba mitochondrial tRNA genes, like their mammalian counterparts, are more variable in sequence than nonorganelle tRNAs. In mitochrondrial protein genes ATG, ATT, ATA, and in one case (COI) ATAA appear to be used as translation initiation codons. The only termination codon found in these genes is TAA. In theD. yakuba mitochondrial genetic code, AGA, ATA, and TGA specify serine, isoleucine, and tryptophan, respectively. Fifty-nine types of sense codon are used in theD. yakuba mitochondrial protein genes, but 93.8% of all codons end in A or T. Codon-anticodon interactions may include both G-A and C-A pairing in the wobble position. Evidence is summarized that supports the hypothesis that A and T nucleotides are favored at all locations in theD. yakuba mtDNA molecule where these nucleotides are compatible with function.
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  • 72
    Digitale Medien
    Digitale Medien
    Springer
    Journal of molecular evolution 35 (1992), S. 51-59 
    ISSN: 1432-1432
    Schlagwort(e): Drosophila ; Gart locus ; Chironomus tentans ; Purine nucleotide biosynthesis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The Drosophila Gart locus consists of two genes. One gene encodes three enzymes in the de novo purine nucleotide biosynthesis pathway [glycinamide ribonucleotide synthetase (GARS), aminoimidazole ribonucleotide synthetase (AIRS), and glycinamide ribonucleotide transformylase (GART)]. The second gene lies within an intron of the purine gene and encodes a cuticle protein. To investigate the evolution of the Gart locus, the Chironomus tentans homolog was cloned by screening a genomic DNA library with a polymerase chain reaction product. This study shows that the interesting structural features of this locus conserved in two distant Drosophila species are not found in the Chironomus homolog. These features include the cuticle protein gene nested within an intron and the existence of an alternative transcript to yield a monofunctional enzyme. In addition, the extremely rapid divergence of coding sequence seen for members of the tandemly duplicated AIRS domain in Drosophila is found to be much less rapid in Chironomus.
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  • 73
    Digitale Medien
    Digitale Medien
    Springer
    Journal of molecular evolution 37 (1993), S. 483-495 
    ISSN: 1432-1432
    Schlagwort(e): Drosophila ; mastermind ; Gene comparison ; Triplet repeat ; Homopolymer ; Protein evolution ; Repeat length variation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Runs of identical amino acids encoded by triplet repeats (homopolymers) are components of numerous proteins, yet their role is poorly understood. Large numbers of homopolymers are present in the Drosophila melanogaster mastermind (mam) protein surrounding several unique charged amino acid clusters. Comparison of mam sequences from D. virilis and D. melanogaster reveals a high level of amino acid conservation in the charged clusters. In contrast, significant divergence is found in repetitive regions resulting from numerous amino acid replacements and large insertions and deletions. It appears that repetitive regions are under less selective pressure than unique regions, consistent with the idea that homopolymers act as flexible spacers separating functional domains in proteins. Notwithstanding extensive length variation in intervening homopolymers, there is extreme conservation of the amino acid spacing of specific charge clusters. The results support a model where homopolymer length variability is constrained by natural selection.
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  • 74
    ISSN: 1432-1432
    Schlagwort(e): Drosophila ; per gene ; Threonine-Glycine ; repeat sequence ; melanogaster subgroup phylogeny
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The Threonine-Glycine (Thr-Gly) region of the period gene (per) in Drosophila was compared in the eight species of the D. melanogaster subgroup. This region can be divided into a diverged variable-length segment which is flanked by more conserved sequences. The number of amino acids encoded in the variable-length region ranges from 40 in D. teissieri to 69 in D. mauritiana. This is similar to the range found within natural populations of D. melanogaster. It was possible to derive a Thr-Gly “allele” of one species from that of another by invoking hypothetical Thr-Gly intermediates. A phylogeny based on the more conserved flanking sequences was produced. The results highlighted some of the problems which are encountered when highly polymorphic genes are used to infer phylogenies of closely related species.
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  • 75
    Digitale Medien
    Digitale Medien
    Springer
    Journal of molecular evolution 32 (1991), S. 421-428 
    ISSN: 1432-1432
    Schlagwort(e): Alcohol dehydrogenase ; Amino acid sequence ; Phylogenetic relationships ; Drosophila
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Increasing data onDrosophila alcohol dehydrogenase (ADH) sequences have made it possible to calculate the rate of amino acid replacement per year, which is 1.7×10−9. This value makes this protein suitable for reconstructing phylogenetic relationships within the genus for those species for which no molecular data are available such asScaptodrosophila. The amino acid sequence ofDrosophila lebanonensis is compared to all of the already knownDrosophila ADHs, stressing the unique characteristic features of this protein such as the conservation of an initiating methionine at the N-terminus, the unique replacement of a glycine by an alanine at a very conserved position in the NAD domain of all dehydrogenases, the lack of a slowmigrating peptide, and the total conservation of the maximally hydrophilic peptide. The functional significance of these features is discussed. Although the percent amino acid identity of the ADH molecule inDrosophila decreases as the number of sequences compared increases, the conservation of residue type in terms of size and hydrophobocity for the ADH molecule is shown to be very high throughout the genusDrosophila. The distance matrix and parsimony methods used to establish the phylogenetic relationships ofD. lebanonensis show that the three subgenera,Scaptodrosophila, Drosophila, andSophophora separated at approximately the same time.
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  • 76
    ISSN: 1432-1432
    Schlagwort(e): Drosophila ; dec-1 locus ; Restriction map conservation ; Sequence comparison ; Melanogaster species subgroup ; Phylogeny
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary We have analyzed ∼18 kb of DNA in and upstream of thedefective chorion-1 (dec-1) locus of the eight known species of themelanogaster species subgroup ofDrosophila. The restriction maps ofD. simulans, D. mauritiana, D. sechellia, D. erecta, andD. orena are shown to have basically the restriction map ofD. melanogaster, whereas the maps ofD. teissieri andD. yakuba were more difficult to align. However, the basic amount of DNA and sequence arrangement appear to have been conserved in these species. A small deletion of varying length (65–200 bp) is found in a repeated sequence of the central transcribed region ofD. melanogaster, D. simulans, andD. erecta. Restriction site mapping indicated that thedec-1 gene is highly conserved in themelanogaster species subgroup. However, sequence comparison revealed that the amount of nucleotide and amino acid substitution in the repeated region is much larger than in the 5′ translated region. The 5′ flanking region showed noticeable restriction site polymorphisms between species. Based on calculations from the restriction maps a dendrogram was derived that supports earlier published phylogenetic relationships within themelanogaster species subgroup except that theerecta-orena pair is placed closer to themelanogaster complex than toD. teissieri andD. yakuba.
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  • 77
    Digitale Medien
    Digitale Medien
    Springer
    Journal of molecular evolution 33 (1991), S. 450-456 
    ISSN: 1432-1432
    Schlagwort(e): Drosophila ; Mariner ; Transposable elements
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The abundance of the transposable elementmariner differs dramatically in the genomes of the closely related speciesDrosophila simulans, D. mauritiana, D. sechellia, andD. melanogaster. Natural populations ofD. simulans andD. mauritiana have 1–10 and 20–30 copies per diploid genome, respectively, and the insertion sites are polymorphic. The element has not been found inD. melanogaster. In this paper we show thatD. sechellia, a species endemic to the Seychelles Islands, contains only twomariner elements that are at fixed sites in the genome. One element, inserted in chromosome 2R at 51A1–2, contains three deletions and is missing much of the 3′ end. The other element, inserted in chromosome 3L at 64A10–11, is the full length of 1286 bp. Although the sequence of the full-length element is polymorphic in populations ofD. sechellia, at least some of the sequences are closely related to elements fromD. simulans andD. mauritiana that are known to be active. However, judging from the progeny of crosses betweenD. sechellia andD. simulans, the biological activity of the full-lengthD. sechellia element appears to be low, either because of the nucleotide sequence of the element or because of its position in the genome, or both.
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  • 78
    Digitale Medien
    Digitale Medien
    Springer
    Journal of molecular evolution 37 (1993), S. 525-543 
    ISSN: 1432-1432
    Schlagwort(e): Drosophila ; Zaprionus ; Phylogeny ; Ribosomal RNA sequences
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Nucleotide sequences of 72 species of Drosophilidae were determined for divergent D1 and D2 domains (representing 200 and 341 nucleotides respectively in D. melanogaster) of large ribosomal RNA, using the rRNA direct sequencing method. Molecular phylogenetic trees were reconstructed using both distance and parsimony methods and the robustness of the nodes was evaluated by the bootstrap procedure. The trees obtained by these methods revealed four main lineages or clades which do not correspond to the taxonomical hierarchy. In our results, the genus Chymomyza is associated with the subgenus Scaptodrosophila of the genus Drosophila and their cluster constitutes the most ancient clade. The two other clades are constituted of groups belonging to the subgenus Sophophora of the genus Drosophila: the so-called Neotropical clade including the willistoni and saltans groups and the obscura-melanogaster clade itself split into three lineages: (1) obscura group + ananassae subgroup, (2) montium subgroup, and (3) melanogaster + Oriental subgroups. The fourth clade, the Drosophila one, contains three lineages. D. polychaeta, D. iri, and D. fraburu are branched together and constitute the most ancient lineage; the second lineage includes the annulimana, bromeliae, dreyfusi, melanica, mesophragmatica, repleta, robusta, and virilis groups. The third lineage is composed of the immigrans and the cardini, funebris, guaramunu, guarani, histrio, pallidipennis, quinaria, and tripunctata groups. The genera Samoaia, Scaptomyza, and Zaprionus are branched within the Drosophila clade. Although these four clades appear regularly in almost all tree calculations, additional sequencing will be necessary to determine their precise relationships.
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  • 79
    Digitale Medien
    Digitale Medien
    Springer
    Journal of molecular evolution 34 (1992), S. 130-140 
    ISSN: 1432-1432
    Schlagwort(e): Drosophila ; Sophophora ; cDNA-DNA hybridization ; Phylogenetics
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary We have performed DNA-DNA hybridization experiments among several species of Drosophila using the evolutionarily conserved portion of the genome representing sequences coding for amino acids of proteins. This was done by using as tracer, radioactively labeled complementary DNA that was reverse transcribed from adult mRNA. We show that this procedure extends phylogenetically the distance over which the technique can be applied to fast-evolving groups such as Drosophila. The major phylogenetic conclusions are (1) the subgenus Sophophora is a monophyletic lineage; (2) within Sophophora the melanogaster subgroup is closer to the obscura group than either group is to the willistoni group; (3) the subgenus Drosophila is complex with most major lineages originating deep in the phylogeny; the subgenus may not be monophyletic; (4) as with most groups classically placed in Drosophila, the Hawaiian Drosophila originate early, supporting the notion that this lineage is older than the extant islands; and (5) the virilis/repleta lineage is monophyletic within Drosophila.
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  • 80
    ISSN: 1432-1432
    Schlagwort(e): Drosophila ; dec-1 eggshell gene ; Wild-type variants ; Repeated region ; DNA sequencing
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Thedec-1 eggshell gene inDrosophila melanogaster encodes follicle cell proteins required for proper eggshell assembly. As shown by Southern and Northern analyses thedec-1 gene occurs in four alleles (Fcl-4) among wild-type strains. Its second exon has a distinct feature in the form of 12 repeats with 78–91 nucleotides; the first five show nearly 100% homology. DNA sequence comparison of the repeated region of the alleles revealed that the length polymorphisms are caused by changes in the numbers of the first five repeats. The results suggest that the alleles have been generated by unequal intragenic crossing-over and/or slippage during DNA replication and that the allelic length variants have arisen independently. The possiblilty that the most common allele,FC1, has a selective advantage over the other alleles is discussed.
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  • 81
    Digitale Medien
    Digitale Medien
    Springer
    Journal of molecular evolution 39 (1994), S. 478-488 
    ISSN: 1432-1432
    Schlagwort(e): Mitochondrial DNA ; Nucleotide sequences ; Drosophila ; Rapid phyletic radiation ; Molecular phylogeny
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Approximately 2 kb corresponding to different regions of the mtDNA of 14 different species of the obscura group of Drosophila have been sequenced. In spite of the uncertainties arising in the phylogenetic reconstruction due to a restrictive selection toward a high mtDNA A+T content, all the phylogenetic analysis carried out clearly indicate that the obscura group is formed by, at least, four well-defined lineages that would have appeared as the consequence of a rapid phyletic radiation. Two of the lineages correspond to monophyletic subgroups (i.e., afftnis and pseudoobscura), whereas the obscura subgroup remains heterogeneous assemblage that could be reasonably subdivided into at least two complexes (i.e., subobscura and obscura).
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  • 82
    ISSN: 1432-1432
    Schlagwort(e): Gene structure ; Heat shock ; hsp70 ; Antiparallel ORFs ; Drosophila
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A clone isolated from a Drosophila auraria heat-shock cDNA library presents two long, antiparallel, coupled (LAC) open reading frames (ORFs). One strand ORF is 1,929 nucleotides long and exhibits great identity (87.5% at the nucleotide level and 94% at the amino acid level) with the hsp70 gene copies of D. melanogaster, while the second strand ORF, in antiparallel in-frame register arrangement, is 1,839 nucleotides long and exhibits 32% identity with a putative, recently identified, NAD+-dependent glutamate dehydrogenase (NAD+-GDH). The overlap of the two ORFs is 1,824 nucleotides long. Computational analysis shows that this LAC ORF arrangement is conserved in other hsp70 loci in a wide range of organisms, raising questions about possible evolutionary benefits of such a peculiar genomic organization.
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  • 83
    ISSN: 1432-1432
    Schlagwort(e): Concerted evolution ; Molecular drive ; Drosophila ; rDNA spacers ; PCR length polymorphism ; MVR-PCR mapping
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Polymerase chain reaction (PCR)-amplified, sequenced, and digitally typed intergenic spacers (IGSs) of the ribosomal (r)DNA in D. melanogaster reveal unexpected features of the mechanisms of turnover involved with the concerted evolution of the gene family. Characterization of the structure of three isolated IGS length variants reveals breakage “hot spots” within the 330-base-pair (bp) subrepeat array found in the spacers. Internal mapping of variant repeats within the 240-bp subrepeat array using a novel digital DNA typing procedure (minisatellite variant repeat [MVR]-PCR) shows an unexpected pattern of clustering of variant repeats. Each 240-bp subrepeat array consists of essentially two halves with the repeats in each half identified by specific mutations. This bipartite structure, observed in a cloned IGS unit, in the majority of genomic DNA of laboratory and wild flies and in PCR-amplified products, has been widely homogenized yet is not predicted by a model of unequal crossing over with randomly placed recombination breakpoints. Furthermore, wild populations contain large numbers of length variants in contrast to uniformly shared length variants in laboratory stocks. High numbers of length variants coupled to the observation of a homogenized bipartite structure of the 240-bp subrepeat array suggest that the unit of turnover and homogenization is smaller than the IGS and might involve gene conversion. The use of PCR for the structural analysis of members of the rDNA gene family coupled to digital DNA typing provides powerful new inroads into the mechanisms of DNA turnover affecting the course of molecular evolution in this family.
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  • 84
    ISSN: 1432-1432
    Schlagwort(e): Drosophila ; Lcp gene family ; Gene homogenization ; CAAT-box motifs ; Gene phylogeny ; Sex chromosomes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The larval cuticle protein genes (Lcps) represent a multigene family located at the right arm of the metacentric autosome 2 (2R) inDrosophila melanogaster. Due to a chromosome fusion theLcp locus ofDrosophila miranda is situated on a pair of secondary sex chromosomes, theX2 andneo-Y chromosome. Comparing the DNA sequences fromD. miranda andD. melanogaster organization and the gene arrangement ofLcp1-Lcp4 are similar, although the intergene distances vary considerably. The greatest difference betweenLcp1 andLcp2 is due to the occurrence of a pseudogene inD. melanogaster which is not present inD. miranda. Thus the cluster of the fourLcp genes existed already before the separation of themelanogaster andobscura group. Intraspecific homogenizations of different cluster units must have occurred repeatedly between theLcp1/Lcp2 andLcp3/Lcp4 sequence types. The most obvious example is exon 2 of theLcp3 gene inD. miranda, which has been substituted by the corresponding section of theLcp4 gene rather recently. The homogenization must have occurred before the translocation which generated theneo-Y chromosome.Lcp3 ofD. melanogaster has therefore no orthologous partner inD. miranda. Rearrangements in the promoter regions of theD. miranda Lcp genes have generated new, potentially functional CAAT-box motifs. Since three of theLcp alleles on theneo-Y are not expressed andLcp3 is expressed only at a reduced level, it is suggestive to speculate that the rearrangements might be involved ascis-regulatory elements in the up-regulation of theX2-chromosomalLcp alleles, inDrosophila an essential process for dosage compensation. TheLcp genes on theneo-Y chromosome have accumulated more base substitutions than the corresponding alleles on theX2.
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  • 85
    ISSN: 1432-1432
    Schlagwort(e): Drosophila ; Cuticle proteins (LCP) ; Conserved protein motifs ; Gene phylogeny ; Sex chromosomes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The larval cuticle proteins (LCPs) are encoded by a multigene family,Lcp1-4, located at the right arm of the metacentric autosome 2 (2R) inDrosophila melanogaster. Due to a chromosome fusion theLcp locus ofDrosophila miranda is situated on a pair of secondary sex chromosomes, theX2 andneo-Y chromosomes. Comparing the deduced amino acid sequences of the autosomalD. melanogaster loci with the sex-chromosomal loci ofD. miranda, we were able to trace the evolution of theLcp loci with respect to their different chromosomal inheritance. The length of the signal peptide is conserved in all four LCPs, while the size of the mature LCPs varies. Conserved protein motifs became obvious from the alignment, indicating regions of structural and functional importance. Analyzing intra- and interspecific sequence similarities of theLcp gene families allowed us to reconstruct the phylogeny of the gene cluster. Alignment with cuticle amino acid sequences originating from divergent insect species reveals motifs already present in the primordial insect LCPs. These motifs indicate different levels of constraint acting during the evolution of the LCPs.
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  • 86
    Digitale Medien
    Digitale Medien
    Springer
    Journal of molecular evolution 9 (1977), S. 279-298 
    ISSN: 1432-1432
    Schlagwort(e): 5S RNA ; Drosophila ; Evolution ; Secondary structure ; Development
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The nucleotide sequence ofDrosophila melanogaster 5S RNA has been determined and appears to be homogeneous both in the KC cell line and in the insect at different developmental stages. Experimental evidence on the conformation of this molecule is in agreement with a general class of 5S RNA models.
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  • 87
    ISSN: 1432-1432
    Schlagwort(e): Histone H2A ; Histone variant ; Intron position ; Drosophila ; Tetrahymena
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Genomic clones ofDrosophila andTetrahymena histone H2A variants were isolated using the corresponding cDNA clones, (van Daal et al. 1988; White et al. 1988). The site corresponding to the initiation of transcription was defined by primer extension for bothDrosophila andTetrahymena genomic sequences. The sequences of the genomic clones revealed the presence of introns in each of the genes. TheDrosophila gene has three introns: one immediately following the initiation codon, one between amino acids 26 and 27 (gln and phe), and one between amino acids 64 and 65 (glu and val). TheTetrahymena gene has two introns, the positions of which are identical to the first two introns of theDrosophila gene. The chicken H2A.F variant gene has been recently sequenced and it contains four introns (Dalton et al. 1989). The first three of these are in the same positions as the introns in theDrosophila gene. The fourth intron interrupts amino acid 108 (gly). In all cases the sizes and the sequences of the introns are divergent. However, the fact that they are in conserved positions suggests that at least two of the introns were present in the ancestral gene. A phylogenetic tree constructed from the sequences of the variant and major cell cycle-regulated histone H2A proteins from several species indicates that the H2A variant proteins are evolutionarily separate and distinct from the major cell cycle-regulated histone H2A proteins. The ancestral H2A gene must have duplicated and diverged before fungi and ciliates diverged from the rest of the eukaryote lineage. In addition, it appears that the variant histone H2A proteins analyzed here are more conserved than the major histone H2A proteins.
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  • 88
    Digitale Medien
    Digitale Medien
    Springer
    Journal of molecular evolution 32 (1991), S. 454-462 
    ISSN: 1432-1432
    Schlagwort(e): Alcohol dehydrogenase gene ; Drosophila ; Gene structure ; Evolutionary trends ; Nucleotide substitution rate
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The study of individual genes is essential to a comprehensive understanding of genome evolution. The wealth of information on alcohol dehydrogenase (Adh) inDrosophila makes this gene particularly suitable for such analysis. We have characterized more than 4 kb of the genomicAdh region inDrosophila ambigua and compared this region toDrosophila mauritiana andDrosophila pseudoobscura. The presence of two genes,Adh and 3′ORF (open reading frame), has been confirmed and some of their essential features have been inferred from primary structural analysis. Inter- and intraspecific comparisons have led us to support that both genes may have diverged from an ancient precursor. They appear to be evolving independently, and show a species-specific pattern. TheAdh in theobscura group species lacks amino acids three and four when compared to the species of themelanogaster group and has accumulated most of its amino acid replacements in the third exon. Neither characteristic is observed when any other group species are compared, which suggests that these may be particular features of the evolution of theobscura group. The 3′ORF is highly conserved among the three species analyzed, although variability in the length of the third exon and the nucleotide substitution rate, which is much higher than inAdh, are worth noting. According to our data, both mutation/fixation rates and the distribution of mutations vary over time, which makes it difficult to predict the evolutionary dynamics of specific genome regions.
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  • 89
    Digitale Medien
    Digitale Medien
    Springer
    Journal of molecular evolution 33 (1991), S. 514-524 
    ISSN: 1432-1432
    Schlagwort(e): Drosophila ; Zaprionus ; Mariner ; Transposable elements ; Horizontal gene transfer ; Alcohol dehydrogenase (Adh) ; Sequence divergence
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The transposable element mariner occurs widely in themelanogaster species group ofDrosophila. However, in drosophilids outside of themelanogaster species group, sequences showing strong DNA hybridization with mariner are found only in the genusZaprionus. the mariner sequence obtained fromZaprionus tuberculatus is 97% identical with that fromDrosophila mauritiana, a member of themelanogaster species subgroup, whereas a mariner sequence isolated fromDrosophila tsacasi is only 92% identical with that fromD. mauritiana. BecauseD. tsacasi is much more closely related toD. mauritiana than isZaprionus, the presence of mariner inZaprionus may result from horizontal transfer. In order to confirm lack of a close phylogenetic relationship between the genusZaprionus and themelanogaster species group, we compared the alcohol dehydrogenase (Adh) sequences among these species. The results show that the coding region of Adh is only 82% identical betweenZ. tuberculatus andD. mauritiana, as compared with 90% identical betweenD. tsacasi andD. mauritiana. Furthermore, the mariner gene phylogeny obtained by maximum likelihood and maximum parsimony analyses is discordant with the species phylogeny estimated by using the Adh genes. The only inconsistency in the mariner gene phylogeny is in the placement of theZaprionus mariner sequence, which clusters with mariner fromDrosophila teissieri andDrosophila yakuba in themelanogaster species subgroup. These results strongly suggest horizontal transfer.
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  • 90
    Digitale Medien
    Digitale Medien
    Springer
    Journal of molecular evolution 36 (1993), S. 315-326 
    ISSN: 1432-1432
    Schlagwort(e): Drosophila ; Fushi tarazu ; Functional constraints ; Regulatory elements
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary We have studied the evolutionary changes occurring in the noncoding regions around the developmentally important fushi tarazu (ftz) gene in a total of 11 species in the genus Drosophila. Previous molecular developmental studies have identified DNA elements both 3′ and 5′ to the coding region which are important in proper regulation of expression of the Drosophila melanogaster ftz gene. We show here that these same elements are the most evolutionarily conserved regions in the vicinity of the gene homologs. Parts of some control elements are more conserved than exonic sequences. Not only is there sequence conservation, but the relative position, orientation, and distances among the control elements remain conserved. One quite significant difference does exist between the two major subgenera studied, Sophophora and Drosophila: namely, an inversion of the ftz unit with respect to other genes in the Antennapedia complex, ANT-C. As a comparison, we applied similar analysis to a “housekeeping” gene-rosy (ry), or Xdh. In contrast, DNA sequences 5′ to the ry coding region revealed little evolutionary conservation. These studies bear out the proposition that functionally important DNA sequences remain more conserved through evolutionary time than do less functionally important sequences. This proposition could be tested in the present case because we could predict a priori from the developmental studies which DNA regions should be most conserved.
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  • 91
    Digitale Medien
    Digitale Medien
    Springer
    Journal of molecular evolution 39 (1994), S. 452-465 
    ISSN: 1432-1432
    Schlagwort(e): Drosophila ; Y chromosome ; Male fertility genes ; Lampbrush loops ; Germ line ; Transposable elements ; Gypsy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract During the evolution of the Y chromosome of Drosophila hydei, retrotransposons became incorporated into the lampbrush loop pairs formed by several of the male fertility genes on this chromosome. Although insertions of retrotransposons are involved in many spontaneous mutations, they do not affect the functions of these genes. We have sequenced gypsy elements that are expressed as constituents of male fertility gene Q in the lampbrush loop pair Nooses. We find that these gypsy elements are all truncated and specifically lost those sequences that may interfere with the continuity of lampbrush loop transcription. Only defective coding regions are found within the loop. Gypsy is not transcribed in loops of many other Drosophila species harboring the family. These results suggest that any contribution of gypsy to the function of male fertility gene Q does not depend on a conserved DNA sequence.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 92
    ISSN: 1432-1432
    Schlagwort(e): Drosophila ; Two-dimensional electrophoresis ; Gonadal protein divergence ; Postzygotic reproductive isolation ; Speciation ; Hybrid sterility
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The possible association between gonadal protein divergence and postzygotic reproductive isolation was investigated among species of the Drosophila melanogaster and D. virilis groups. Protein divergence was scored by high-resolution two-dimensional electrophoresis (2DE). Close to 500 protein spots from gonadal tissues (testis and ovary) and nongonadal tissues (malpighian tubules and brain) were analyzed and protein divergence was calculated based on presence vs absence. Both testis and ovary proteins showed higher divergence than nongonadal proteins, and also a highly significant positive correlation with postzygotic reproductive isolation but a weaker correlation with prezygotic reproductive isolation. Particularly, a positive and significant correlation was found between proteins expressed in the testis and postzygotic reproductive isolation among closely related species such as the within-phylad species in the D. virilis group. The high levels of male-reproductive-tract protein divergence between species might be associated with F1 hybrid male sterility among closely related species. If so, a lower level of ovary protein divergence should be expected on the basis that F1 female hybrids are fully fertile. However, this is not necessarily true if relatively few genes are responsible for the reproductive isolation observed between closely related species, as recent studies seem to suggest. We suggest that the faster rate of evolution of gonadal proteins in comparison to nongonadal proteins and the association of that rate with postzygotic reproductive isolation may be the result of episodic and/or sexual selection on male and female molecular traits.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 93
    Digitale Medien
    Digitale Medien
    Springer
    Journal of molecular evolution 41 (1995), S. 615-621 
    ISSN: 1432-1432
    Schlagwort(e): Drosophila ; Ribosomal genes ; Sequence data
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract We compare the 5S gene structure from nine Drosophila species. New sequence data (5S genes of D. melanogaster, D. mauritiana, D. sechellia, D. yakuba, D. erecta, D. orena, and D. takahashii) and already-published data (5S genes of D. melanogaster, D. simulans, and D. teissieri) are used in these comparisons. We show that four regions within the Drosophila 5S genes display distinct rates of evolution: the coding region (120 bp), the 5′-flanking region (54–55 bp), the 3′-flanking region (21–22 bp), and the internal spacer (149–206 bp). Intra- and interspecific heterogeneity is due mainly to insertions and deletions of 6–17-bp oligomers. These small rearrangements could be generated by fork slippages during replication and could produce rapid sequence divergence in a limited number of steps.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 94
    ISSN: 1432-1351
    Schlagwort(e): Photoreception ; Extraretinal Photoreceptor ; Chromophore ; Opsin ; Reptile ; Immunocytochemistry ; HPLC
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract Since the beginning of this century evidence has accumulated which demonstrates that non-mammalian vertebrates possess photoreceptors situated deep within the brain. While many attempts have been made to localize these sensory cells, studies have either failed or been inconclusive. In this report we have used several experimental approaches to localize the deep brain photoreceptors of the lizard Anolis carolinensis. Using 3 antibodies that bind vertebrate cone opsins, we have immunolabelled cerebrospinal fluid (CSF)-contacting neurons located at the ventricular border within the nucleus ventromedialis of the septum. Western blot analysis indicates that these antibodies recognized a single 40 kD protein in ocular, anterior brain, and pineal extracts. Immunoblots of rodent brain did not show a similar protein band. We have also identified specific retinoids associated with phototransduction (11-cis and all-trans-3,4-didehydroretinaldehyde) within anterior brain extracts. This combined data provides the most detailed analysis of deep brain photoreceptors in any vertebrate. Consequently, we feel Anolis provides an excellent model to study this unexplored sensory system of the vertebrates.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 95
    Digitale Medien
    Digitale Medien
    Springer
    Journal of comparative physiology 175 (1994), S. 587-596 
    ISSN: 1432-1351
    Schlagwort(e): Wing beat frequency ; Optomotor responses ; Landing response ; Drosophila
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract The present study shows that the wing beat frequency of Drosophila is visually controlled and modulated in response to different optomotor stimuli. Whereas rotational large field stimuli do not appear to modulate wing beat frequency, single rotating vertical stripes increase or decrease wing beat frequency when moving back-to-front or front-to-back, respectively. Maximal modulations occur at lateral stripe positions. Expansion stimuli eliciting the landing response cause a marked increase in wing beat frequency. Parameters of this frequency response depend in a graded fashion on certain stimulus properties, and the frequency response co-habituates with the landing response. Several results indicate that the frequency response is an integral component of the landing response, although it can also occur when the characteristic front leg extension is not observed. The complex spatial input integration underlying the frequency response and other motor components of the landing response cannot easily be explained by a system of large field integration units, but might indicate the existence of local expansion detectors.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 96
    Digitale Medien
    Digitale Medien
    Springer
    Journal of comparative physiology 176 (1995), S. 355-364 
    ISSN: 1432-1351
    Schlagwort(e): Drosophila ; Giant fiber ; Escape Olfactory ; Flight
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract We have monitored the patterns of activation of five muscles during flight initiation of Drosophila melanogaster: the tergotrochanteral muscle (a mesothoracic leg extensor), dorsal longitudinal muscles #3, #4 and #6 (wing depressors), and dorsal ventral muscle #Ic (a wing elevator). Stimulation of a pair of large descending interneurons, the giant fibers, activates these muscles in a stereotypic pattern and is thought to evoke escape flight initiation. To investigate the role of the giant fibers in coordinating flight initiation, we have compared the patterns of muscle activation evoked by giant fiber stimulation with those during flight initiations executed voluntarily and evoked by visual and olfactory stimuli. Visually elicited flight initiations exhibit patterns of muscle activation indistinguishable from those evoked by giant fiber stimulation. Olfactory-induced flight initiations exhibit patterns of muscle activation similar to those during voluntary flight initiations. Yet only some benzaldehyde-induced and voluntary flight initiations exhibit patterns of muscle activation similar to those evoked by giant fiber stimulation. These results indicate that visually elicited flight initiations are coordinated by the giant fiber circuit. By contrast, the giant fiber circuit alone cannot account for the patterns of muscle activation observed during the majority of olfactory-induced and voluntary flight initiations.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 97
    Digitale Medien
    Digitale Medien
    Springer
    Journal of comparative physiology 176 (1995), S. 611-623 
    ISSN: 1432-1351
    Schlagwort(e): Ligand-gated channel ; Chloride channel ; Histamine ; Drosophila ; Visual ecology
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract The large monopolar cells (LMCs) of the first optic neuropil (lamina) in insects respond to the photoreceptor neurotransmitter histamine with an increase in chloride conductance. We have compared the properties of this conductance from a range of diptera from different visual environments: Tipula paludosa (slow flying, crepuscular), Drosophila melanogaster (slow-flying diurnal), and 3 fast-flying diurnal species Musca domestica, Calliphora vicina and Lucilia sericata. In whole-cell recordings of dissociated LMCs, histamine-induced currents were elicited using a multichannel parallel perfusion device, allowing rapid determination of the dose-response function, characterised by affinity (K d) and Hill coefficient (n). Calliphora, Lucilia and Musca had the steepest dose response curves (n = 2.8) and the lowest affinity for histamine (K d 35–50 μM); the crepuscular Tipula had a significantly higher affinity (K d = 16 μM) and lower Hill coefficient (n = 1.8). Drosophila had a high affinity (K d 24 μM), and a high Hill coefficient (n = 2.5). In excised inside-out patch recordings all species showed similar single channel properties (conductance 40–60 pS, mean open time 〈 1 ms). The low Hill coefficient in Tipula would be expected to result in lower synaptic gain. We suggest this may be an adaptation to prevent the LMC's response bandwidth being filled with the high levels of photon noise typical of photoreceptors adapted for low light levels. The lower affinity for histamine found in the more photopic species suggests that the concentration of histamine (and therefore presumably number of synaptic vesicles released from the photoreceptors) should be higher. This might improve signal-to-noise ratio by decreasing the contribution of the shot event noise introduced by stochastic release of synaptic vesicles.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 98
    Digitale Medien
    Digitale Medien
    Springer
    Journal of comparative physiology 178 (1996), S. 513-522 
    ISSN: 1432-1351
    Schlagwort(e): Potassium channel ; Photoreceptor ; Calliphora ; Drosophila ; Shaker
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract Single electrode current and voltage clamp recordings in Calliphora, and whole-cell voltage clamp recordings in Drosophila were used to characterise the voltage-gated K channels in both major classes of photoreceptors, R7/8 (long visual fibres, LVFs) and R1-6 (short visual fibres, SVFs). R7/8 were identified by their unique spectral properties, ca. 3–4 fold higher input resistances and 3–4 fold lower cell capacitance. In Calliphora SVFs possess both fast and slow activating delayed rectifier potassium conductances. Drosophila SVFs possess a slowly inactivating delayed rectifier (IKs), a very rapidly inactivating A channel encoded by the Shaker gene (IA), and, in a minority of cells, a third K conductance with intermediate kinetics (IKf). In both specs the LVFs lack the slowest component, but exhibit the faster K conductance(s) with properties indistinguishable from those in the SVFs. These findings add to established evidence demonstrating the significant role played by potassium channels in tuning the photoreceptor membrane. The results also suggest that R1-6 photoreceptors and R7/8 form inputs to visual subsystems tuned to different temporal frequencies.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 99
    Digitale Medien
    Digitale Medien
    Springer
    Journal of comparative physiology 172 (1993), S. 303-308 
    ISSN: 1432-1351
    Schlagwort(e): Drosophila ; Photoreception ; Magnetoreception ; Magnetic compass orientation ; Geomagnetic field
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract 1. Wildtype Oregon-R Drosophila melanogaster were trained in the ambient magnetic field to a horizontal gradient of 365 nm light emanating from one of the 4 cardinal compass directions and were subsequently tested in a visually-symmetrical, radial 8-arm maze in which the magnetic field alignment could be varied. When tested under 365 nm light, flies exhibited consistent magnetic compass orientation in the direction from which light had emanated in training. 2. When the data were analyzed by sex, males exhibited a strong and consistent magnetic compass response while females were randomly oriented with respect to the magnetic field. 3. When tested under 500 nm light of the same quantal flux, females were again randomly oriented with respect to the magnetic field, while males exhibited a 90° clockwise shift in magnetic compass orientation relative to the trained direction. 4. This wavelength-dependent shift in the direction of magnetic compass orientation suggests that Drosophila may utilize a light-dependent magnetic compass similar to that demonstrated previously in an amphibian. However, the data do not exclude the alternative hypothesis that a change in the wavelength of light has a non-specific effect on the flies' behavior, i.e., causing the flies to exhibit a different form of magnetic orientation behavior.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 100
    Digitale Medien
    Digitale Medien
    Springer
    Journal of comparative physiology 175 (1994), S. 179-191 
    ISSN: 1432-1351
    Schlagwort(e): Drosophila ; Neuromuscular ; Haemolymph ; Membrane potential ; Synaptic potential
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract Neuromuscular preparations from third instar larvae of Drosophila are not well-maintained in commonly used physiological solutions: vacuoles form in the muscle fibers, and membrane potential declines. These problems may result from the Na∶K ratio and total divalent cation content of these physiological solutions being quite different from those of haemolymph. Accordingly haemolymph-like solutions, based upon ion measurements of major cations, were developed and tested. Haemolymph-like solutions maintained the membrane potential at a relatively constant level, and prolonged the physiological life of the preparations. Synaptic transmission was well-maintained in haemolymph-like solutions, but the excitatory synaptic potentials had a slower time course and summated more effectively with repetitive stimulation, than in standard Drosophila solutions. Voltage-clamp experiments suggest that these effects are linked to more pronounced activation of muscle fiber membrane conductances in standard solutions, rather than to differences in passive muscle membrane properties or changes in postsynaptic receptor channel kinetics. Calcium dependence of transmitter release was steep in both standard and haemolymph-like solutions, but higher external calcium concentrations were required for a given level of release in haemolymph-like solutions. Thus, haemolymph-like solutions allow for prolonged, stable recording of synaptic transmission.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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