ALBERT

Description: Galectins (GAL) are β-galactoside binding proteins. The objective of this study was to assess the effect of PMA on the regulation of galectin gene expression in bovine blood and neutrophils. Blood was collected from 3 clinically healthy Holstein cows. Neutrophils were isolated by differential centrifugation. Blood and neutrophils were treated with 10ng/ml of PMA or maintained in phosphate-buffered saline for 30 minutes at 370 C. Pooled total RNA were transcribed to cDNA for real-time PCR. Specific primers for bovine LGALS -1, -2, -3, -4, -7, -8, -9, -11, -12, and their binding proteins (bp) LGALS3bp, T cell immunoglobulin and mucin domain 3 (TIM-3) were used. Housekeeping genes RPLP0 and UCHL5 served as internal controls. Fold changes were calculated using the Livak method. Total protein concentration was assessed using the Bicinchoninic acid assay. Secretion of GAL -1, -2, -3, -4, -8, -9 was assessed using bovine GAL specific ELISA. All tested LGALS were detected. Activation with PMA differentially modulated expression and secretion of GAL in blood and neutrophils. Expression levels of LGALS -1 and LGALS -3 was not affected. Expression of LGALS -7, -8, -9, -11, -12, and TIM-3 was differentially modulated. The secretion of GAL -3 was significantly increased in response to PMA. The most pronounced effect of PMA was observed on LGALS-9, TIM-3 expression, and the secretion of GAL -3. Thus, GAL may serve as biomarkers of cell activation with functional consequences and warrant further study.

Description: Background: Systemic inflammation is believed to have an important role in pathogenesis of Chronic Obstructive Pulmonary Disease (COPD) and its related factors should be considered in monitoring of the disease. In the current study, possible link between inflammatory status and various related factors in patients with COPD was assessed. Method: Sixty-one COPD patients according to the inclusion criteria participated in this study. For assessing nutritional status, SGA (subjective global assessment) and 24-hour dietary recall method were used and Health-related quality of life (HRQoL) was assessed by St. George’s respiratory questionnaire (SGRQ), instrumental activities of daily living scales (IADLs), and Katz Index. Moreover, Anthropometric and body composition measurements including weight, height, BMI, FFM, and FFMI were measured by standard methods and BIA. Additionally, muscle strength was assessed using a hydraulic hand dynamometer. Finally, blood samples were collected to assess biochemical factors including TNF-α, IL-6, MDA, vitamin C, magnesium, and Glutathione. Stepwise model was performed for evaluating the relationship between inflammatory markers (TNF-α and IL-6) and associated markers mentioned above. Characteristics of participants were expressed in percentage and mean ±SD and analyzed by SPSS software. Results: The results of the current study showed that the intake of PUFA and vegetables, plasma vitamin C and serum MDA could possibly affect inflammation according to IL-6 and TNF- α concentrations. On the other hand, systemic inflammation (IL-6 and TNF-) aggravated mean right and left handgrip strength, Katz index and nutritional status (SGA score) significantly (P

Description: Stuttering is a male-biased speech motor control disorder that lead to disruption in the rhythm of speech. The effect of sex on development of stuttering is well known; males are more susceptible to and less recovered from stuttering than female. Sex hormones have been studied as a main accused factor for this gender dependency of the disorder. The aim of this systematic review is to navigate the extent of previous research about the relationship of developmental stuttering and sex hormones. Toward these ends, a comprehensive, electronic review of past concepts regarding the relationship of stuttering with sex hormones and digit ratio as an indirect index for fetal testosterone exposure, in Scopus, Science Direct, PubMed, Medline, Embase, and Cochrane database was carried out to identify potential studies for the review. Inclusion criteria were original quantitative research, written in English, used human subjects and published from 2000 through 2020. Findings were mixed, although potential patterns were identified. There were methodological limitations such as small participant numbers, in the targeted population in this review research. The findings from this current study add to the growing body of evidence demonstrating that sex hormone have a significant association with stuttering.

Description: In this study natural carbohydrates such as glucose, galactose, xylose, fructose andlactose, are acetylated by acetic anhydride and sodium acetate catalyst. Anomeric configuration is deacetylated by (i-Pr)3Sn(OEt)as a catalyst, an easy synthetic regioselective deacetylation of full acetylated carbohydrates using (i--Pr)3Sn(OEt) is described. The acetylated carbohydrates reacted with HBr (solution in AcOH, 32 wt.%) for the bromination of anomeric position. The synthesis oxazaphosphorine, and bromo hexa alkyl Methylsulfonate derivatives from anomeric position of carbohydrates was reacted. FT IR, 1H, 13C NMR, 31PNMR spectroscopy techniques were employed to examine the synthesized compounds.

Description: Background and Objective: Inhalation of chemicals and toxins in cigarette and hookah smoke results in loss of integrity of oral cavity tissues. The objective of this study was to compare the periodontal health of hookah and cigarette smokers. Materials and Methods: In this study, 73 men at the age group of 20-35 years who smoked hookah for more than 5 years and 73 men at the same age who smoked cigarette for more than 5 years and 73 healthy men referred to the periodontal department of Mashhad dentistry school were selected and studied. Periodontal indices including pocket depth, GI, BOP, CAL were assessed in two groups. Also, using parallel periapical radiography, mesial and distal marginal bone level around the first molar tooth was measured. Finally, the data were assessed and compared using appropriate statistical analysis. Results: Pocket depth was 24.27% and 23.62%, respectively, in cigarette smokers and hookah smokers, and it was 0.96% in healthy subjects, clinical attachment level was 4.48 and 4.41 mm, respectively, in cigarette smokers and hookah smokers, and it was 0.77 mm in healthy subjects. Gingival index (GI) was 1.40 and 1.42, respectively, in cigarette smokers and hookah smokers and it was 0.52 in healthy subjects, BOP value ​​was 6.52 and 6.52%, respectively, in cigarette smokers and hookah smokers and it was 10.86% in healthy subjects. Mesial marginal bone level of first lower molar tooth was 2.27 and 2.32 mm, respectively, in cigarette smokers and hookah smokers and it was 1.74 mm in healthy subjects and distal marginal bone level of lower first molar tooth was 2.38 and 2.35 mm, respectively, in cigarette smokers and hookah smokers and it was 1.75 mm in healthy subjects. Comparison of results between the two groups of cigarette smokers and hookah smokers did not show any significant relationship in any of the variables, but there was a significant relationship between the two groups and the healthy group (P

Description: Background: Although many GTN patients can be treated with chemotherapy, a small proportion of them will relapse after complete recovery. To the best of our knowledge, there is not any information in respect of relapsed GTN cases in our region. In the current study we have aimed to evaluation of the recurrence risk of gestational trophoblastic neoplasia (GTN) after serum βhCG normalization Methods: This descriptive-analytical study was carried out on registered hospital data of patients with confirmed GTN diagnosis following molar pregnancy who admitted to the gynecology ward of Imam Khomeini Hospital between 2011 and 2017. Patients with diagnosis of postmolar GTN, based on at least five bhcg measurements was included. Patients information including initial level of serum BhcG, time to Bhcg resolution, types of molar pregnancy, treatment protocols, need to recuretage relapse, and finally, the period time between bhcg resolution to relapse were evaluated. Results: In the present study, 239 patients with GTN (including 180 complete and 59 partial moles) were evaluated. The mean age of the patients was 28.8 years, ranging from 16 to 47 years. The mean βhCG concentration was 170,000 IU/ml (ranged 760 to 850,000). The mean time of βhCG resolution was 8.19 months in the range of 4 to 12 months. Recurrence was observed in 9 patients (3.7%). The mean period time between βhCG resolution to relapse was 20.94 months. The mean initial level of βhCG was significantly lower in patients with recurrence (p

Description: Background. Malnutrition is a major health problem in India. It mostly affects children of school going age with serious health problems putting them at an increased risk for developing chronic diseases later in life. This study aims to study the prevalence of malnutrition (overweight and underweight) in children aged 10-13 years in schools in Manipal and Utopia, Karnataka. Materials and Methods. In this study, a total 400 students were studied. Their anthropometric measurements like height, weight, BMI and MUAC, dietary pattern, physical activity level, and child eating behavior. Questionnaire was used to note the student’s information. The data collected was subjected to statistical analysis using SPSS version 21, Diet Cal, and Anthroplus1.0.4. Results. It was observed that in the childhood category 10-13 years there were 222 (55.5%) male and 178(44.5%) female. According to the BMI for age 53.4% of them falls under the normal category; 22.3% falls under the mild wasting category; About 18.3% falls under the obese category and 8% falls under the moderate wasting category. There is no students falling under the severe wasting and overweight category according to the BAZ classification. When the height for age was done for all the children, about 36.3% falls under normal height; About 35.5% falls under mild stunting; 19.3% falls under the moderate stunting; 8.3% falls under the severe stunting category; and 1% falls under tall category according to the HAZ classification. Conclusions. In conclusion, mild stunting and wasting was observed among children. There are no students falling under the severe wasting and overweight category according to the BAZ classification.

Description: Background. Due to the significant and Irrefutable effects of parental style on children and the lack of a comprehensive studies on this topic, the present study aimed to study the relation between parenting style and attitude with physical activity, diet behavior and health of children (10-13 years). Materials and Methods. This study is a descriptive cross - sectional study and conducted in schools located in Manipal and Udupi (Karnataka, India) during May 2017 to November 2017. A well designed questionnaire was administrated to children which included general information, along with physical activity questionnaire. The questionnaire also comprised of various other anthropometric measurements like BMI, MUAC, and Waist hip Circumferences etc. The data collected was subjected to statistical analysis using SPSS version 21. BMI has been calculated using anthroplus 1.0.4. Results. The results of this study showed that there is a significant relationship between parenting style and diet behavior of the child (P〈 0.001). Parents with stylistic dimension in both authoritative and authoritarian have more influence on child for food avoidance and food approach. Studies shows an association between authoritarian and permissive parenting style with food approach behaviors and child weight status of children. parenting style is not affected on Childs physical activity. 74.8% of variation in weight is explained by the independent factors like parenting style, Nutrient intake, Food group intake, Physical activity level, Anthropometric measurement, and child eating behaviour questionnaire. There was a significant relationship between weight and these factors at 1 % level (F=32.889, P〈 0.001). Conclusions. In conclusion, parents with stylistic dimension in both authoritarian and permissive style of parenting and parents with stylistic dimensions in all type of parenting style influences child physical activity. Parents with stylistic dimension in both authoritative and authoritarian have more influence on child for food avoidance and food approach behavior.

Description: Introduction: Cystoscopy is one of the common procedure in diagnosis and treatment of problem related to urinary tract, pre-cystoscopy anxiety is a very common problem, despite evidences shows suitable intervention for post-cystoscopy pain but enough pain relief still remains as a problem, the aim of this study was to investigate the relationship between pre-cystoscopy anxiety and post-cystoscopy pain among patients who was hospitalized for cystoscopy. Methods: This was a cross sectional study and sampling of this study was done in April 2017 to April 2018 in surgical unit of amiralmoemenin hospital in zanjan, Iran, using non-probability convenience Sampling method, 61 patients aged 16-45 years old who was hospitalized for cystoscopy was recruited in this study. Pre-cystoscopy anxiety was assessed by spilberger (STAI) questionnaire and post-cystoscopy pain measured by pain visual analogs scale (VAS). Results: The mean (SD) age of men and females were 43.81 (14.68), 44.88 (13.61) years old, respectively The relationship between state anxiety and post cystoscopy pain was direct and significant (p

Description: Background: Gestational trophoblastic neoplasia (GTN), despite its widespread metastases, is a very common cancer in women that is curable. Although the GTN cases show a good response to chemotherapy, in an effort to reduce toxic drug exposure, the second curettage has been suggested for some patients. In the current study, we have aimed to compare the benefits of the second curettage in comparison with single-agent chemotherapy for low-risk GTN patients. Methods: This retrospective observational study was carried out on GTN patients admitted to the gynecology department of Imam Khomeini Hospital in Ahvaz. The demographic profile of all participants was extracted. Patients' hospitalization records were also extracted from the files. Patients with an endometrial thickness above 10 mm were treated with re-curettage. The β hCG clearance time was estimated by the Kaplan Meier plot. Results: In the present study, 148 patients with low-risk GTN stage 1 were studied. The time required for β-hCG clearance in patients undergoing re-curettage was significantly lower than the chemotherapy receiving group (7 months vs. 10 months, p