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  • 1
    Electronic Resource
    Electronic Resource
    DAPI fluorescence of plant chromosomes prestained with actinomycin D
    Amsterdam : Elsevier
    Experimental Cell Research 102 (1976), S. 408-413 
    Details
    ISSN: 0014-4827
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0014-4827(76)90057-4
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  • 2
    Electronic Resource
    Electronic Resource
    Modification of DAPI banding on human chromosomes by prestaining with a DNA-binding oligopeptide antibiotic, distamycin A
    Amsterdam : Elsevier
    Experimental Cell Research 111 (1978), S. 327-332 
    Details
    ISSN: 0014-4827
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0014-4827(78)90177-5
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  • 3
    Electronic Resource
    Electronic Resource
    Heterochromatin diversity in Cymbidium, and its relationship to differential DNA replication
    Amsterdam : Elsevier
    Experimental Cell Research 98 (1976), S. 411-423 
    Details
    ISSN: 0014-4827
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0014-4827(76)90450-X
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  • 4
    Electronic Resource
    Electronic Resource
    Repetitious DNA in some Anemone Species (1974)
    Springer
    Chromosoma 44 (1974), S. 417-421 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The DNA from several Anemone species, which contain different amounts of heterochromatin as revealed by Giemsa staining, was analysed by ultra-centrifugation and renaturation. No satellite band was observed in any of the samples centrifuged in cesium chloride gradients. Renaturation studies showed the presence of repetitive sequences. The proportion of repetitive DNA per genome varied from 53% to 67% and did not correlate with either the DNA content per cell or the relative amount of heterochromatin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00284901
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  • 5
    Electronic Resource
    Electronic Resource
    Giemsa banding: karyotype differences in some species of Anemone and in Hepatica nobilis (1974)
    Springer
    Chromosoma 44 (1974), S. 405-416 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Banding patterns were revealed in the karyotypes of six species of Anemone and in Hepatica nobilis using a Giemsa staining technique. — There was considerable inter-specific variation both regarding the amount and distribution of bands. Small but significant intra-specific differences in banding patterns were also found. The results are discussed both as they relate to the use of Giemsa banding in karyotype analysis and to understanding the nature of the banding phenomenon itself.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00284900
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  • 6
    Electronic Resource
    Electronic Resource
    Heterochromatin and nucleolus-organizer-region behaviour at male pachytene of Sus scrofa domestica (1984)
    Springer
    Chromosoma 91 (1984), S. 12-19 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract In the domestic pig (2n=38) two types of constitutive heterochromatin can be differentiated by fluorescence counterstaining techniques. All 24 biarmed autosomes and the X chromosome have chromomycin A3-positive centromeric C-bands, whereas all 12 acrocentric chromosomes exhibit DA-DAPI-positive centromeric heterochromatin. Fluorescence analysis of male pachytene nuclei revealed that the DA-DAPI-positive C-bands form one or two large chromocentres per cell, while the chromomycin A3-bright C-material is well scattered. Hence, the bivalents formed by the acrocentric chromosome pairs are centromerically associated, whilst the submetacentric bivalents are not. —Counce-Meyer spreading techniques were used to study the structure of synaptonemal complexes (SCs) both by light and electron microscopy. In general, the SCs of the domestic pig resemble those described for other mammals. The SC formed by the X and the Y may include up to 94.5% of the Y chromosome. In silver-stained microspreads each of the bivalents (nos. 8 and 10) bearing the nucleolus-organizer-regions (NORs) is connected to a pair of nucleoli, indicating that all four NORs are active during early meiotic stages. By contrast, in the majority of mitotic metaphases of phytohaemagglutinin-stimulated lymphocytes only one pair (no. 10) exhibited Ag-NOR staining. — The significance of the chromosome disposition in the pachytene nucleus is discussed with regard to heterochromatin composition and karyotype evolution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00286480
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  • 7
    Electronic Resource
    Electronic Resource
    Cytogenetics of the parthenogenetic grasshopper Warramaba virgo and its bisexual relatives (1983)
    Springer
    Chromosoma 88 (1983), S. 227-236 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Combinations of DNA-binding fluorescent dyes and counterstains that enhance selectivity and contrast in primary stain fluorescence were used to differentiate types of C-bands in the genus Warramaba. Chromomycin A3 (in conjunction with two A-T binding counterstains), which identifies chromosome segments enriched in G-C base pair clusters, stains only a minority of the C-bands in Warramaba species, but these include all those known to contain 18S + 26S rRNA cistrons and most of those containing 5S rRNA genes. DAPI/actinomycin D fluorescent staining is positive for a very few bands, including two (in the Standard phylad of W. virgo) that are at or adjacent to sites containing 5S rRNA cistrons. One of the latter regions is also positively stained by DAPI/distamycin A which, in addition, highlights some centromeric bands. The fluorescent staining patterns of the Standard and Boulder-Zanthus phylads of W. virgo are significantly different, confirming their independent origin by hybridization between different races of the ancestral species “P169” and “P196”.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00285625
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  • 8
    Electronic Resource
    Electronic Resource
    Errata (1978)
    Springer
    Chromosoma 65 (1978), S. 391-391 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00286418
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  • 9
    Electronic Resource
    Electronic Resource
    A 41–42 bp tandemly repeated sequence isolated from nuclear envelopes of chicken erythrocytes is located predominantly on microchromosomes (1990)
    Springer
    Chromosoma 99 (1990), S. 131-137 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract To study whether specific DNA sequences are associated with nuclear membranes, residual DNA was extracted from DNase-treated nuclear envelopes prepared from erythrocytes of adult chickens (Gallus domesticus). This DNA was then blunt-end ligated into a bacterial plasmid vector. DNA blot analysis and nucleotide sequence determination revealed that approximately 30% of the cloned fragments consisted of different multiples of a 41–42 bp tandemly repeated, partially symmetrical sequence. In situ hybridization to chicken chromosomes demonstrated that the sequence was located primarily on microchromosomes, although some hybridization was also observed to macrochromosomes 7 and 8. Digestion of chicken DNA with any of a number of restriction enzymes did not completely reduce the intensity of a high molecular weight band to which the repeated sequence hybridized. These results, along with those obtained from in situ hybridization, suggested that many copies of this sequence are organized into large tandem arrays, and are not dispersed in many shorter repetitive blocks throughout the chicken genome. Although the repetitive sequence constituted approximately 10% of the chicken genome, it did not hybridize to quail or turkey DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01735329
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  • 10
    Electronic Resource
    Electronic Resource
    Meiotic chromosome behaviour reflects levels of sequence divergence inSus scrofa domestica satellite DNA (1990)
    Springer
    Chromosoma 99 (1990), S. 330-335 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We present a general model for the evolution of chromosome-specific satellite DNA subfamilies.Sus scrofa domestica has a bimodal karyotype with two autosomal subsets of 12 meta-/submetacentric (Mc) and 6 acrocentric (Ac) chromosome types (Mc and Ac “subgenomes”). We show that the centromeric heterochromatin is characterised by two distinct satellite DNA families designed Mc1 and Ac2. Mc1 is a diverse satellite family of the Mc subgenome of which certain members with a 100 bp repeat unit are found to occur at the pericentromeric regions of each Mc autosome, while others are chromosome-specific, e.g. clone Mc pAv1.5, a higher order repeat variant, which hybridises specifically to chromosome 1. Ac2 is a homogeneous satellite occurring at the subterminal pericentromeric regions of all Ac autosomes. DNA sequence analyses showed that all clones investigated are built up from a 14 bp repeat unit which is highly conserved. In situ hybridisation to meiotic pachytene nuclei revealed a distinct spatial arrangement of the Ac2 centromeric satellite.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01731720
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