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  • 1
    Electronic Resource
    Electronic Resource
    Alteration of Industrial Food and Beverage Yeasts by Recombinant DNA Technology (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 646 (1991), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1991.tb18574.x
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  • 2
    Electronic Resource
    Electronic Resource
    Human interleukin-13 activates the interleukin-4-dependent transcription factor NF-IL4 sharing a DNA binding motif with an interferon-γ-induced nuclear binding factor
    Amsterdam : Elsevier
    FEBS Letters 345 (1994), S. 187-192 
    Details
    ISSN: 0014-5793
    Keywords: Interferon-γ ; Interleukin-13 ; Interleukin-4 ; Nuclear binding factor ; Responsive element ; Signal transduction
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0014-5793(94)00438-2
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  • 3
    Electronic Resource
    Electronic Resource
    FLP-FRT mediated intrachromosomal recombination on a tandemly duplicated YEp integrant at the ILV2 locus of chromosome XIII in Saccharomyces cerevisiae (1989)
    Springer
    Current genetics 15 (1989), S. 107-112 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; 2μm FRT duplication ; Intrachromosomal recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A YEp chimaeric plasmid carrying SMR1 and URA3 genetic markers was integrated into chromosome XIII at the ilv2-Δ1 locus in a [cir°] background. The 1.5 kb BglII deletion of ilv2-Δ1 allowed the clear identification of an integrant structure which consisted of a direct tandem duplication (TD) of the chimaeric plasmid. Within the integrant structure, a single copy of the plasmid sequence was flanked by a direct duplication of the 2μm site-specific recombinase (FLP) recognition target (FRT). Isogenic [cir°] and [cir +] diploids formed by crossing the [cir°] TD strain to complementary haploids were analyzed for plasmid marker loss and chromosomal DNA alterations in the presence and absence of selection pressure for the URA3 and SMR1 plasmid borne markers. [cir°] diploids showed no plasmid marker loss and maintained the TD structure. In the absence of selection pressure, the [cir +] diploid underwent FLP-FRT mediated unequal interchromatid recombination, resulting in the breakage-fusion-bridge cycle and homozygotization of chromosome XIII (Rank et al. 1988). Maintenance of selection pressure for the centromere distal plasmid URA3 marker selected against FLP-FRT interchromatid recombinants so that the effects of site specific recombinase on intrachromatid recombination could be evaluated. Intrachromatid recombination at the directly duplicated FRT sites of the TD structure resulted in the loss of a diagnostic internal fragment. These results show that in the presence of FLP, FRT sites separated by up to 13.3 kb of chromosomal DNA function as substrates for intra and interchromatid recombination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00435456
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  • 4
    Electronic Resource
    Electronic Resource
    High viscosity vesicles of yeast separated at p H 4 have surface glycoprotein (1978)
    [s.l.] : Nature Publishing Group
    Nature 273 (1978), S. 682-684 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] We used the procedure described by Fuhrmann et al.2 for the isolation of plasma membrane vesicles capable of galactose transport. A 5,000g pellet from mechanically disrupted cells was resuspended at 1 mg protein ml"1 in ice cold osmotic stabiliser (400mMKCl, lmMMgCl2, 20 mM tri-ethanolamine). The ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/273682a0
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  • 5
    Electronic Resource
    Electronic Resource
    FLP recombinase induction of the breakage-fusion-bridge cycle and gene conversion in Saccharomyces cerevisiae (1988)
    Springer
    Current genetics 13 (1988), S. 273-281 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; FLP-FRT ; BFBC ; Gene conversion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A YEp chimaeric plasmid containing URA3 and SMR1 [sulfometuron methyl resistant (SMR) allele of ILV2] as selectable markers, and the 2 μm site-specific recombination FLP recognition target (FRT), was integrated at the ilv2-Δ1 site in chromosome XIII in a cir°] haploid. Southern analysis defined two integrant structures. Structure I had URA3 distal and SMR1 proximal to FRT whereas in structure II both markers were distal to FRT. Selectable markers were stably inherited in [cir°] haploids and [cir°] diploids heterozygous for the integrant and ILV2. Approximately 14% of heterozygous [cir +] diploid cells exhibited homozygotization for the distal (500 kb) ade4 marker in trans. In [cir +] diploids FLP-FRT recombination resulted in the simultaneous loss of both structure II markers, whereas the structure I distal URA3 marker loss always preceded the variable loss of the proximal SMR1 marker. URA− cells continued to segregate for loss of SMR1 until stable URA− SMR or URA−SMS cells were produced. Gene conversion was identified in stable URA−SMR cells that were homozygous SMR1/SMR1 but contained wild type ILV2 restriction endonuclease sites. These observations support a model based on concerted FLP-FRT action resulting from the secondary integration of native 2 μm DNA followed by unequal sister chromatid exchange (USCE) within inverted FRTs. The resultant chromatid bridge resulted in a double-stand break. Fusion of the broken ends of sister chromatids generated a breakage-fusion-bridge cycle (BFBC). Repeated rounds of the BFBC resulted in proximal marker loss and the generation of additional double-strand breaks. Recombinogenic properties of the double-strand break initiated events leading to homozygotization and gene conversion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00424420
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  • 6
    Electronic Resource
    Electronic Resource
    Polymorphism within the nuclear and 2μm genomes of Saccharomyces cerevisiae (1991)
    Springer
    Current genetics 20 (1991), S. 189-194 
    Details
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Bakers' and lager yeast ; Chromosomal and 2 μm DNA polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Seven strains of bakers' yeast were obtained as a representative sample of the Spanish baking industry. The nuclear genome was monitored for polymorphism by transverse alternating field electrophoresis (TAFE) and restriction maps of 2 μm DNA were produced. All seven strains were uniquely different when evaluated by their total chromosomal lengths whereas only two 2 μm variants were defined. There was no apparent correlation between chromosomal and plasmid polymorphism. The extensive chromosomal polymorphism within one 2 μm DNA type indicates the rapid and relatively recent evolution of the nuclear genome. The hybrid origin (S. cerevisiae-S.monacensis) of lager yeast was critically evaluated by TAFE analysis of S. cerevisiae and S. carlsbergensis chromosomes. The absence of corresponding S. cerevisiae chromosomes III and XIII in S. carlsbergensis argued against the hybrid origin of lager strains. We discuss limitations of the hybrid origin hypothesis of industrial yeasts and propose that the molecular coevolution observed in 2 μm DNA serves as a useful additional mechanism for rationalization of some of the structural polymorphism of the nuclear genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00326231
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  • 7
    Electronic Resource
    Electronic Resource
    Antisense RNA regulation of the ILV2 gene in yeast: a correction (1994)
    Springer
    Current genetics 25 (1994), S. 289-289 
    Details
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Inducible antisense gene ; Acetolactate synthase ; Bradytrophic phenocopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A previous report of the use of antisense RNA to regulate gene expression in yeast is incorrect.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00357175
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  • 8
    Electronic Resource
    Electronic Resource
    A transient duplication of the acetolactate synthase gene in a cell culture of Datura innoxia (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 417-422 
    Details
    ISSN: 1432-2242
    Keywords: Gene amplification ; Datura ; Sulfonylurea resistance ; Plant cell culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A 2.0 kb fragment of the yeast ILV2 gene, which codes for the target enzyme acetolactate synthase (ALS) of the herbicide chlorsulfuron, was shown to hybridize to the nuclear DNA of a haploid cell culture of Datura innoxia P. Mill. Nuclear DNA of a chlorsulfuron resistant line of D. innoxia, CSR6, gave a prominent 2.65 kb band when cleaved by either EcoRI or HindIII. The 2.65 kb band has been shown to hybridize with the yeast ILV2 probe. A herbicide resistant line descended from CSR6 by continuous culture resulted in the loss of the 2.65 kb restriction fragment. These observations suggest that CSR6 resulted from a large tandem duplication of the ALS gene and that a point mutation for herbicide resistance in an ALS gene repeat unit of the duplication was selected during subsequent growth of the resistant line.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00289814
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  • 9
    Electronic Resource
    Electronic Resource
    Single nuclear gene inherited cross resistance and collateral sensitivity to 17 inhibitors of mitochondrial function in S. cerevisiae (1973)
    Springer
    Molecular genetics and genomics 126 (1973), S. 93-102 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Previous tetrad analyses defined a yeast strain (332-7c) as containing a single nuclear gene (11.8 map units from the centromere) conferring resistance to oligomycin. Resistance to 18 additional inhibitors of mitochondrial function (Table 1) was determined on (i) ascospore isolates from tetrads segregating 2 resistant: 2 sensitive for oligomycin (Table 2) and (ii), spontaneously derived sensitive isolates of the oligomycin resistant strain (Tables 3 and 4). The observed pattern of resistance suggests that the gene for resistance to oligomycin also results in (i) cross resistance to rutamycin, venturicidin, triethyltin bromide, antimycin A, carbonylcyanide m-chlorophenylhydrazone, tetra-N-butylammonium bromide, dibenzyl-dimethylammonium chlorop and tetracycline and (ii), collateral sensitivity to paromomycin, neomycin, dequalinium chloride, ethidium bromide and acriflavin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330986
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  • 10
    Electronic Resource
    Electronic Resource
    The bivious suppressiveness of cytoplasmic petites of S. cerevisiae lacking in mitochondrial DNA (1973)
    Springer
    Molecular genetics and genomics 120 (1973), S. 115-124 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary When crossed to strain GR25 [rho+], petites lacking in mtDNA were neutral, but when crossed to a related [rho+] strain (GR25a) they were found to be suppressive (Table 1). Likewise, crosses of GR25 [rho+] and GR25a [rho+] to a common [rho+] parent were, respectively, neutral and suppressive (Table 1). The suppressive phenotype observed in these crosses was attributed to a factor in the [rho+] strain GR25a. Strain GR25a also differed from strain GR25 in having a decreased [rho+] stability (Table 2) and a decreased transmission of its cytoplasmically-inherited erythromycin-resistance marker to zygote progeny (Table 4). These three phenotypes of GR25a are, discussed in terms of a nuclear mutation in a gene responsible for the maintenance of the [rho+] state.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267239
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