Publication Date:
1998-12-16
Description:
After synthesis and processing in the nucleus, mature transfer RNAs (tRNAs) are exported to the cytoplasm in a Ran.guanosine triphosphate-dependent manner. Export of defective or immature tRNAs is avoided by monitoring both structure and function of tRNAs in the nucleus, and only tRNAs with mature 5' and 3' ends are exported. All tRNAs examined can be aminoacylated in nuclei of Xenopus oocytes, thereby providing a possible mechanism for functional proofreading of newly made tRNAs. Inhibition of aminoacylation of a specific tRNA retards its appearance in the cytoplasm, indicating that nuclear aminoacylation promotes efficient export.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lund, E -- Dahlberg, J E -- GM30220/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1998 Dec 11;282(5396):2082-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biomolecular Chemistry, University of Wisconsin-Madison, 1300 University Avenue, Madison, WI 53-706, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9851929" target="_blank"〉PubMed〈/a〉
Keywords:
Acylation
;
Animals
;
Biological Transport
;
Cell Nucleus/*metabolism
;
Cytoplasm/metabolism
;
Introns
;
Nucleic Acid Conformation
;
Oocytes
;
RNA Precursors/chemistry/*metabolism
;
RNA Processing, Post-Transcriptional
;
RNA Splicing
;
RNA, Transfer/chemistry/*metabolism
;
RNA, Transfer, Amino Acid-Specific
;
RNA, Transfer, Amino Acyl/chemistry/*metabolism
;
RNA, Transfer, Met/chemistry/metabolism
;
RNA, Transfer, Tyr/chemistry/metabolism
;
Templates, Genetic
;
Xenopus laevis
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
