Publication Date:
1998-04-16
Description:
The genomic regulatory network that controls gene expression ultimately determines form and function in each species. The operational nature of the regulatory programming specified in cis-regulatory DNA sequence was determined from a detailed functional analysis of a sea urchin control element that directs the expression of a gene in the endoderm during development. Spatial expression and repression, and the changing rate of transcription of this gene, are mediated by a complex and extended cis-regulatory system. The system may be typical of developmental cis-regulatory apparatus. All of its activities are integrated in the proximal element, which contains seven target sites for DNA binding proteins. A quantitative computational model of this regulatory element was constructed that explicitly reveals the logical interrelations hard-wired into the DNA.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Yuh, C H -- Bolouri, H -- Davidson, E H -- New York, N.Y. -- Science. 1998 Mar 20;279(5358):1896-902.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9506933" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Base Sequence
;
Binding Sites
;
Cell Adhesion Molecules/*genetics/physiology
;
Computer Simulation
;
DNA-Binding Proteins/metabolism
;
Embryo, Nonmammalian/metabolism
;
Endoderm/metabolism
;
Gastrula/metabolism
;
*Gene Expression Regulation, Developmental
;
Lithium Chloride/pharmacology
;
Models, Genetic
;
Molecular Sequence Data
;
Mutagenesis
;
Promoter Regions, Genetic/genetics/*physiology
;
Proteins/*genetics/physiology
;
Sea Urchins/embryology/*genetics/metabolism
;
*Transcription, Genetic/drug effects
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
