Publication Date:
1998-03-21
Description:
The nocturnal increase in circulating melatonin in vertebrates is regulated by 10- to 100-fold increases in pineal serotonin N-acetyltransferase (AA-NAT) activity. Changes in the amount of AA-NAT protein were shown to parallel changes in AA-NAT activity. When neural stimulation was switched off by either light exposure or L-propranolol-induced beta-adrenergic blockade, both AA-NAT activity and protein decreased rapidly. Effects of L-propranolol were blocked in vitro by dibutyryl adenosine 3',5'-monophosphate (cAMP) or inhibitors of proteasomal proteolysis. This result indicates that adrenergic-cAMP regulation of AA-NAT is mediated by rapid reversible control of selective proteasomal proteolysis. Similar proteasome-based mechanisms may function widely as selective molecular switches in vertebrate neural systems.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Gastel, J A -- Roseboom, P H -- Rinaldi, P A -- Weller, J L -- Klein, D C -- New York, N.Y. -- Science. 1998 Feb 27;279(5355):1358-60.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Section on Neuroendocrinology, Laboratory of Developmental Neurobiology, National Institute of Child Health and Human Development (NICHD), National Institutes of Health, Bethesda, MD 20892-4480, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9478897" target="_blank"〉PubMed〈/a〉
Keywords:
Adrenergic beta-Agonists/pharmacology
;
Adrenergic beta-Antagonists/pharmacology
;
Animals
;
Arylamine N-Acetyltransferase/*metabolism
;
Bucladesine/pharmacology
;
Cyclic AMP/metabolism
;
Cysteine Endopeptidases/*metabolism
;
Cysteine Proteinase Inhibitors/pharmacology
;
Isoproterenol/pharmacology
;
Light
;
Melatonin/*biosynthesis
;
Multienzyme Complexes/*metabolism
;
Pineal Gland/cytology/drug effects/enzymology/*metabolism
;
Propranolol/pharmacology
;
Proteasome Endopeptidase Complex
;
Rats
;
Receptors, Adrenergic, beta/physiology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
