Publication Date:
1996-12-20
Description:
Genetic footprinting was used to assess the phenotypic effects of Ty1 transposon insertions in 268 predicted genes of chromosome V of Saccharomyces cerevisiae. When seven selection protocols were used, Ty1 insertions in more than half the genes tested (157 of 268) were found to result in a detectable reduction in fitness. Results could not be obtained for fewer than 3 percent of the genes tested (7 of 268). Previously known mutant phenotypes were confirmed, and, for about 30 percent of the genes, new mutant phenotypes were identified.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Smith, V -- Chou, K N -- Lashkari, D -- Botstein, D -- Brown, P O -- 1PO1 HG00205-05/HG/NHGRI NIH HHS/ -- New York, N.Y. -- Science. 1996 Dec 20;274(5295):2069-74.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA. Medicine, Stanford, CA 94305, USA. pbrown@cmgm.stanford.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8953036" target="_blank"〉PubMed〈/a〉
Keywords:
Chromosomes, Fungal/*genetics
;
Cloning, Molecular
;
Culture Media
;
DNA Footprinting
;
DNA Transposable Elements
;
DNA, Fungal/genetics
;
Gene Library
;
*Genes, Fungal
;
Mutagenesis, Insertional
;
Phenotype
;
Polymerase Chain Reaction
;
Saccharomyces cerevisiae/*genetics/growth & development/physiology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
