Publication Date:
1993-01-29
Description:
Ras proteins undergo a series of posttranslational modifications that are critical for their cellular function. These modifications are necessary to anchor Ras proteins to the membrane. Yeast Ras2 proteins were purified with various degrees of modification and examined for their ability to activate their effector, adenylyl cyclase. The farnesylated intermediate form of Ras2 had more than 100 times higher affinity for adenylyl cyclase than for the unprocessed form. The subsequent palmitoylation reaction had little effect. In contrast, palmitoylation was required for efficient membrane localization of the Ras2 protein. These results indicate the importance of farnesylation in the interaction of Ras2 with its effector.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kuroda, Y -- Suzuki, N -- Kataoka, T -- New York, N.Y. -- Science. 1993 Jan 29;259(5095):683-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Physiology, Kobe University School of Medicine, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8430318" target="_blank"〉PubMed〈/a〉
Keywords:
Adenylyl Cyclases/genetics/isolation & purification/*metabolism
;
Amino Acid Sequence
;
Animals
;
Base Sequence
;
Cell Line
;
Cell Membrane/enzymology
;
Electrophoresis, Polyacrylamide Gel
;
Enzyme Activation
;
Fungal Proteins/genetics/isolation & purification/*metabolism
;
GTP-Binding Proteins/genetics/*metabolism
;
Genes, Fungal
;
Guanosine 5'-O-(3-Thiotriphosphate)/metabolism
;
Insects
;
Kinetics
;
Molecular Sequence Data
;
Molecular Weight
;
Oligodeoxyribonucleotides
;
Palmitic Acid
;
Palmitic Acids/metabolism
;
Protein Binding
;
*Protein Processing, Post-Translational
;
Recombinant Fusion Proteins/isolation & purification/metabolism
;
Saccharomyces cerevisiae/genetics/*metabolism
;
*Saccharomyces cerevisiae Proteins
;
Transfection
;
*ras Proteins
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
