Publication Date:
1994-09-16
Description:
To identify proteins that may participate in the activation of the protein kinase Raf, proteins that interact with Raf were selected in a two-hybrid screen. Two members of the 14-3-3 protein family were isolated that interacted with both the amino terminal regulatory regions of Raf and the kinase domain of Raf, but did not compete with the guanine nucleotide-binding protein Ras for binding to Raf. 14-3-3 proteins associated with Raf in mammalian cells and accompanied Raf to the membrane in the presence of activated Ras. In yeast cells expressing Raf and MEK, mammalian 14-3-3 beta or 14-3-3 zeta activated Raf to a similar extent as did expression of Ras. Therefore, 14-3-3 proteins may participate in or be required for the regulation of Raf function. These findings suggest a role for 14-3-3 proteins in Raf-mediated signal transduction.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Freed, E -- Symons, M -- Macdonald, S G -- McCormick, F -- Ruggieri, R -- New York, N.Y. -- Science. 1994 Sep 16;265(5179):1713-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Onyx Pharmaceuticals, Richmond, CA 94806-5206.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8085158" target="_blank"〉PubMed〈/a〉
Keywords:
14-3-3 Proteins
;
Amino Acid Sequence
;
Animals
;
Cell Line
;
Cell Membrane/enzymology
;
Cytosol/enzymology
;
Enzyme Activation
;
GTP-Binding Proteins/metabolism
;
HeLa Cells
;
Humans
;
MAP Kinase Kinase 1
;
*Mitogen-Activated Protein Kinase Kinases
;
Molecular Sequence Data
;
Nerve Tissue Proteins/*metabolism
;
Protein-Serine-Threonine Kinases/chemistry/genetics/*metabolism
;
Protein-Tyrosine Kinases/genetics/metabolism
;
Proto-Oncogene Proteins/chemistry/*metabolism
;
Proto-Oncogene Proteins c-raf
;
Recombinant Proteins/metabolism
;
Saccharomyces cerevisiae/genetics/growth & development
;
Signal Transduction
;
*Tyrosine 3-Monooxygenase
;
Zinc Fingers
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
