Publication Date:
1994-08-26
Description:
Proteasomes degrade endogenous proteins. Two subunits, LMP-2 and LMP-7, are encoded in a region of the major histocompatibility complex (MHC) that is critical for class I-restricted antigen presentation. Mice with a targeted deletion of the gene encoding LMP-7 have reduced levels of MHC class I cell-surface expression and present the endogenous antigen HY inefficiently; addition of peptides to splenocytes deficient in LMP-7 restores wild-type class I expression levels. This demonstrates the involvement of LMP-7 in the MHC class I presentation pathway and suggests that LMP-7 functions as an integral part of the peptide supply machinery.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Fehling, H J -- Swat, W -- Laplace, C -- Kuhn, R -- Rajewsky, K -- Muller, U -- von Boehmer, H -- New York, N.Y. -- Science. 1994 Aug 26;265(5176):1234-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Basel Institute for Immunology, Switzerland.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8066463" target="_blank"〉PubMed〈/a〉
Keywords:
*ATP-Binding Cassette Transporters
;
Amino Acid Sequence
;
Animals
;
Antigen Presentation
;
Antigen-Presenting Cells/immunology
;
Base Sequence
;
Carrier Proteins/genetics
;
*Cysteine Endopeptidases
;
Female
;
Gene Deletion
;
H-2 Antigens/*biosynthesis/immunology
;
H-Y Antigen/immunology
;
Lymphocytes/immunology
;
Male
;
Mice
;
Mice, Knockout
;
Molecular Sequence Data
;
*Multienzyme Complexes
;
Proteasome Endopeptidase Complex
;
Proteins/genetics/*physiology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
