Publication Date:
1994-11-04
Description:
An activity in human cell extracts is described that repairs DNA with loops of five or more unpaired bases. Repair is strand-specific and is directed by a nick located 5' or 3' to the loop. This repair is observed in a colorectal cancer cell line that is devoid of a wild-type hMLH1 gene and is deficient in repair of mismatches. However, a cell line with deletions in both hMSH2 alleles is deficient in repair of both loops and mismatches. Defects in loop repair may be relevant to the repetitive-sequence instability observed in cancers and other hereditary diseases.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Umar, A -- Boyer, J C -- Kunkel, T A -- New York, N.Y. -- Science. 1994 Nov 4;266(5186):814-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7973637" target="_blank"〉PubMed〈/a〉
Keywords:
Adaptor Proteins, Signal Transducing
;
Base Composition
;
Base Sequence
;
Carrier Proteins
;
Cell Extracts
;
Cell Line
;
Colorectal Neoplasms/*genetics
;
*DNA Repair
;
DNA, Satellite/genetics/metabolism
;
*DNA-Binding Proteins
;
HeLa Cells
;
Humans
;
Molecular Sequence Data
;
MutS Homolog 2 Protein
;
Neoplasm Proteins/*genetics/physiology
;
Nuclear Proteins
;
Nucleic Acid Heteroduplexes/*metabolism
;
Proto-Oncogene Proteins/*genetics/physiology
;
Repetitive Sequences, Nucleic Acid
;
Tumor Cells, Cultured
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
