Publication Date:
1994-10-14
Description:
An activity that severs stable microtubules is thought to be involved in microtubule reorganization during the cell cycle. Here, a 48-kilodalton microtubule-severing protein was purified from Xenopus eggs and identified as translational elongation factor 1 alpha (EF-1 alpha). Bacterially expressed human EF-1 alpha also displayed microtubule-severing activity in vitro and, when microinjected into fibroblasts, induced rapid and transient fragmentation of cytoplasmic microtubule arrays. Thus, EF-1 alpha, an essential component of the eukaryotic translational apparatus, appears to have a second role as a regulator of cytoskeletal rearrangements.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Shiina, N -- Gotoh, Y -- Kubomura, N -- Iwamatsu, A -- Nishida, E -- New York, N.Y. -- Science. 1994 Oct 14;266(5183):282-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Genetics and Molecular Biology, Kyoto University, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7939665" target="_blank"〉PubMed〈/a〉
Keywords:
Adenosine Triphosphate/pharmacology
;
Amino Acid Sequence
;
Animals
;
Base Sequence
;
Cell Line
;
Guanosine Triphosphate/analogs & derivatives/metabolism
;
Humans
;
Microtubules/drug effects/*metabolism
;
Molecular Sequence Data
;
Molecular Weight
;
Oocytes
;
Peptide Elongation Factor 1
;
Peptide Elongation Factors/chemistry/isolation & purification/*physiology
;
Rats
;
Recombinant Proteins/pharmacology
;
Ribonucleoproteins/chemistry/isolation & purification/*physiology
;
Sepharose/analogs & derivatives/metabolism
;
Xenopus laevis
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
