Publication Date:
1995-09-15
Description:
The protein tyrosine kinase c-Src is transiently activated at the transition from the G2 phase to mitosis in the cell cycle of mammalian fibroblasts. Fyn and Yes, the other members of the Src family present in fibroblasts, were also found to be activated at mitosis. In cells microinjected with a neutralizing antibody specific for Src, Fyn, and Yes (anti-cst.1) during G2, cell division was inhibited by 75 percent. The block occurred before nuclear envelope breakdown. Antibodies specific for phosphatidylinositol-3 kinase alpha and phospholipase C-gamma 1 had no effect. Microinjection of the Src homology 2 (SH2) domain of Fyn was also inhibitory. Functional redundancy between members of the Src family was observed; a Src-specific antibody had no effect in NIH 3T3 cells but inhibited cell division in fibroblasts in which the only functional Src family kinase was Src itself. Thus, Src family kinases and proteins associating with their SH2 domains are required for entry into mitosis.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Roche, S -- Fumagalli, S -- Courtneidge, S A -- New York, N.Y. -- Science. 1995 Sep 15;269(5230):1567-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institut National de la Sante et de la Recherche Medicale (INSERM) faculte de Pharmacie, Montpellier, France.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7545311" target="_blank"〉PubMed〈/a〉
Keywords:
3T3 Cells
;
Amino Acid Sequence
;
Animals
;
Antibodies, Monoclonal
;
Enzyme Activation
;
*G2 Phase
;
Isoenzymes/immunology/metabolism
;
Mice
;
Microinjections
;
*Mitosis
;
Molecular Sequence Data
;
Nocodazole/pharmacology
;
Phospholipase C gamma
;
Protein-Tyrosine Kinases/immunology/*metabolism
;
Proto-Oncogene Proteins/immunology/*metabolism
;
Proto-Oncogene Proteins c-fyn
;
Proto-Oncogene Proteins c-yes
;
Proto-Oncogene Proteins pp60(c-src)/immunology/metabolism
;
Type C Phospholipases/immunology/metabolism
;
*src-Family Kinases
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
