Publication Date:
1980-12-05
Description:
Flow cytometry of heated sperm nuclei revealed a significant decrease in resistance to in situ denaturation of spermatozoal DNA in samples from bulls, mice, and humans of low or questionable fertility when compared with others of high fertility. Since thermal denaturation of DNA in situ depends on chromatin structure, it is assumed that changes in sperm chromatin conformation may be related to the diminished fertility. Flow cytometry of heated sperm nuclei may provide a new and independent determinant of male fertility.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Evenson, D P -- Darzynkiewicz, Z -- Melamed, M R -- New York, N.Y. -- Science. 1980 Dec 5;210(4474):1131-3.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7444440" target="_blank"〉PubMed〈/a〉
Keywords:
Acridine Orange
;
Animals
;
Cattle
;
Cell Nucleus/ultrastructure
;
Chromatin/*ultrastructure
;
*Fertility
;
Hot Temperature
;
Humans
;
Infertility, Male/*pathology
;
Male
;
Mice
;
Nucleic Acid Denaturation
;
Sperm Head/*ultrastructure
;
Spermatozoa/*physiology/*ultrastructure
;
Zinc/deficiency
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
