Publication Date:
1984-08-03
Description:
Explants of embryonic rat mesencephalon were grown in organotypic culture. Addition of 10 microM 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) to the culture medium for 4 to 7 days resulted in loss of dopamine cell bodies and fiber outgrowths, as observed by fluorescence histochemistry. At the same time, the cultures showed decreased uptake of tritium-labeled dopamine. However, no signs of generalized toxicity were evident when the explant cultures were viewed by light and phase-contrast microscopy. These results show that MPTP exerts a relatively selective destructive action in dopamine neurons in vitro, similar to the action observed in humans and monkeys in vivo. Pargyline (10 microM), a monoamine oxidase inhibitor, protected the dopamine neurons in the explants. Organotypic cultures provide an experimental model for the study of the properties of MPTP in vitro.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Mytilineou, C -- Cohen, G -- NS-11631/NS/NINDS NIH HHS/ -- New York, N.Y. -- Science. 1984 Aug 3;225(4661):529-31.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6610939" target="_blank"〉PubMed〈/a〉
Keywords:
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
;
Animals
;
Dopamine/*metabolism
;
Embryo, Mammalian
;
Histocytochemistry
;
Mesencephalon/*drug effects/physiology
;
Neurons/*drug effects/physiology
;
Organ Culture Techniques
;
Pyridines/*toxicity
;
Rats
;
Tritium
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
